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Abstract

The volatiles chemical composition and biological attributes of coriander (Coriandrum sativum L.) leaves essential oil obtained by two extraction techniques namely supercritical fluid extraction and hydro-distillation is appraised. The coriander essential oil yield (.12%) by hydro-distillation was slightly higher than that of supercritical fluid extraction (.09%). The physico-chemical variables of the essential oil obtained from both the techniques varied in significantly (P < .05). GC-MS analysis identified 23 different components in supercritical fluid extracted oil and 18 components in hydro-distilled essential oil having linalool as major component (51.32% and 61.78%, respectively) followed by phytol (12.71%). The oil recovered by supercritical fluid extraction exhibited greater DPPH radical scavenging activity as well as reducing power as compared to the essential oil obtained by hydro-distillation technique along with a stronger biofilm inhibition and least hemolysis. The results of antimicrobial activity revealed that super critical fluid extracted essential oil has potent antifungal and antibacterial activity against P. multocida and A alternata, whereas hydro-distilled essential oil displayed better antimicrobial potential against E coli and A niger. Overall, these results depict that supercritical fluid extraction is superior than hydro-distillation with regard to isolation of better-quality coriander essential oil for nutra-pharmaceutical developments.

Keywords

hydro-distilled coriander oil supercritical fluid extraction linalool gas chromatography-mass spectrometry natural antioxidant antimicrobials agents

Introduction

Coriander (Coriandrum sativum L.), belonging to the family Apiaceae (Umbelliferae), has been described as one of the oldest herbs used for culinary and medicinal purposes.^1,2 A native to Italy, this species is now broadly cultivated in different zones of the world such as North Africa, Russia, Central and Eastern Europe, Asia (Pakistan, India, Bangladesh, and China), and Mediterranean regions (Egypt, Morocco, and Malta).^3,4 It can be grown throughout the year, but the best growth is observed between the months of October and February and flowers are produced between June and July.⁵ Because of its uses as a flavoring agent in foods and cosmetics, this annual herb has a greater economic value. The fruit and seeds of coriander are aromatic, having a mild bittersweet and spicy taste.⁶ The dried coriander seeds are employed as a component of curry powder and these are also used in stews and minced meat dishes. The fresh leaves of coriander have often been utilized in salads, making sauces and to garnish the food due to their characteristic aroma and attractive green color.^7,8

The main producers of coriander are Czech Republic, Poland, Romania, Mexico, Argentina, India, and Guatemala.⁵ Countries like Ukraine and India have the major control over the global market and trade of coriander oil.⁹ The oils obtained from coriander are also used in body care products, perfumes, cosmetics, and cosmeceuticals.¹⁰ The coriander seed and fresh leaves were studied to check the occurrence of different biologically actives ingredients which impart various biological and health promoting attributes.^2,11,12 Conventionally, different parts and products of coriander plant have been used to treat gastrointestinal disorder like dyspepsia, diarrhea, pain, anorexia, flatulence, and vomiting.²

A variety of medicinal herbs have been tested for essential oil (EO) production and commercialization.^13,14 In particular, various EOs can be produced from different parts, that is, aerial (flower, buds, stem, leaves, fruits, peels, bark, wood, and seed) and underground (roots) of aromatic plants.^15,16 The majority of odoriferous plants have been investigated to contain EO which can be obtained from dried, partially dehydrated or fresh parts of plants.¹⁶ Chemically, plants EOs mainly contain terpenoids (volatile constituents) which are of great biological importance due to their multiple pharmaceutical applications.¹⁶ The main applications of EOs include their uses as preservatives and flavoring agents, as well as potent bioactive agents.^17,18 Besides the traditional use of EOs as health and healing agents in aromatherapy, these have also been documented as potential ingredient in preparation of flavored foods, perfumery products, cosme-nutraceuticals, and natural therapeutics.^17,19

The processing methods used for the handling and storage of plant materials as may affect the quality and quantity of EOs isolated.²⁰ The plant’s genetic, environmental and physiological factors as well as well as extraction techniques employed can alter the volatiles composition and ultimately the yield and quality of EO extracted from different sources.²¹ Among different techniques, SCFE (supercritical fluid extraction) is employed for the extraction of EOs from different plant materials including basil,¹⁷ sweet gale,²² and jasmine.²³ In this green extraction method, the aromas from the plants are extracted/isolated using supercritical fluid such as liquid CO₂. Carbon dioxide is an inert gas having mild values of critical temperature (31.1°C) and critical pressure (7.38 MPa) and is considered to be non-hazardous and non-toxic. The supercritical fluid extraction method is more selective and safer as compared to conventional methods (hydro-distillation) and the extracted product contains no residual solvent but have value added benefits.²⁴

The Soon valley, situated in the Punjab province of Pakistan, with Sakesar as the highest peak in the Salt Range, covers around 300-square-mile area. The valley has impressive scenic beauty due to presence of several waterfalls, natural lakes and ponds, and lush green mountains. The agro-ecological and agro-climatic characteristics of the Soon valley, in terms of mild temperature, distinct topography, and low rainfall, are unique as compared with the neighboring regions resulting in unique phytochemical composition of the medicinal flora in this specific area.²⁵

In one of our recent studies, we appraised and compared the volatiles composition and biological principles of basil (Ocimum basilicum L.) essential oil obtained by different techniques.¹⁷ As a part of our ongoing research activities, in the present research work, we planned to appraise the unexplored biochemical profile of coriander leaves harvested from unique Soon valley of Punjab, Pakistan. So, this research project is specifically framed to appraise the biological attributes and composition of essential oil obtained from coriander plants grown in this region. As per our best understanding, this report is the first one which compares the yield, volatile bioactives profiling and biological attributes of coriander leaves essential oil produced by two different techniques, that is, hydro-distillation and SCFE.

Experimental

Sample Collection

The plant parts (stem and leaves) of coriander (Coriandrum sativum L.) were obtained during spring season from Soon Valley, District Khushab, Punjab, Pakistan (Coordinates: 32^o58^/N 72^o15^/E). A taxonomist from Botany Department, University of Sargodha, Sargodha, Pakistan, further authenticated the specimen. The collected samples were washed and dried (moisture content 6.50%) at room temperature prior to extraction of EO.

Chemical and Reagents

Free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH), trichloro-acetic acid, ammonium thio-cyanate, ascorbic acid (vitamin C), aluminum chloride (AlCl₃), ferrous chloride, potassium ferri-cyanate, sodium nitrite, ferric chloride, butylatedhydroxytoluene (BHT) (99.0 %), butylatedhydroxyanisole (BHA), dimethyl sulfoxide, 3-(4,5-dimethylthiazol-2,5-diphenyl tetrazolium bromide) (MTT), penicillin/ streptomycin solution, and fluconazole were supplied by Sigma Chemical Corporation, USA. Sterile resazurin tablets for the purpose of sterilization were obtained from BDH Laboratories. All other chemicals such as chloroform, ammonium thiocyanate, ferrous chloride, methanol, and sodium carbonate (anhydrous) used in the research were acquired from Merck, Germany, unless stated otherwise. Analytical grade reagents and chemical were used for this research work.

Recovery of EO

The pulverized coriander leaves samples were processed for the extraction of EO using two different techniques for comparative study: conventional hydro-distillation (HD) technique and modern supercritical fluid extraction (SCFE) technique.

Hydro-Distillation

EO was recovered using Clevenger-type apparatus. For this purpose, plant sample (3 kg) was hydro-distilled for 3 hours. The recovered oil was dehydrated over sodium sulfate (anhydrous). After filtration, recovered oil was preserved at −4°C until analyzed for different experiments.^16,17

Supercritical Fluid Extraction (SCFE)

A commercial SCFE instrument (Deven’ supercritical private, Ltd., India) was used for the recovery of EO from coriander plant material. The plant sample (5 kg) was placed in the extractor and heated for 60 minutes at 45°C before the starting the process. Flow rate of supercritical CO₂ was adjusted as 10 mL/minute, under 100 bar pressure at 45°C. The extraction was done for 90 minutes under static condition and then for 30 minutes under dynamic conditions. The EO was collected at outlet and preserved at −4°C for experimental uses.²⁶

Analysis of Essential Oils

Yield and Physical Analyses

The yield (%) of the isolated EO was determined by weight of initially used plant. The physical analyses of the EOs include color, solubility (in 70% alcohol), density (25°C), and refractive index (25°C). These analyses were conducted following Abbas et al,¹⁷ Density and refractive index of EO were measured by densimeter (Anton Paar, model DMA 602, Austria), digital refractometer RX-7000a (Atago Co. Ltd., Japan), respectively.

Gas Chromatography (GC)

The bioactives chemical profiling of the extracted coriander EO was studied using gas chromatography (PerkinElmer, Model 8700, USA) coupled with FID detector. Capillary column (HP-5MS, 30 m length, .25 mm diameter) was used in which thickness of stationary phase was .25 μm. The temperature of the column was initially adjusted at 80°C (for 3 min), followed by a gradual increase in temperature by 4°C per minute till 220°C, and finally maintained at 220°C for 10 minutes. The mobile phase used was an inert noble gas (He) which was allowed to flow at the rate of 1.5 mL per minute. The sample of EO (1.0 μL) was introduced in the injector (220°C) in a split mode, while detector temperature was maintained at 290°C.

GC-MS Analysis

The volatile bioactive components were further authenticated by using another advanced technique gas chromatography coupled with mass spectrometer (GCMS) (Agilent, model 6890N, MS-5975). For this analysis, the type of column and its temperate programming was the same as previously used in GC-FID method along with the same carrier gas with same flow rate, but here the detection of the components was performed using mass spectrometer with electron ionization mode (70 eV). Identification of separated components was carried out by comparing their mass spectra with those of standard spectra published in NIST mass spectral library and finding out the retention indices (RI) relative to the normal alkane (C₉–C₂₄) and compared with the RI values of authentic compounds.^17,27

Antioxidant Potential

The antioxidant potential of the extracted coriander EO was estimated by performing different assays including reducing potential and free radical scavenging capacity.

DPPH^. Scavenging Assay

A stable free radical, DPPH, was employed to evaluate antioxidant ability of recovered EOs.¹⁷ Various concentration of sample (.5–100 μg/mL) were mixed with 1.0 mL dilute solution of DPPH free radical (90 μmolar) and rectified methanol (95%) to make the total volume of mixture up to 4 mL. Positive control (butylated hydroxy toluene) was processed for comparing with the activity of the sample essential oil. The final solution was incubated and OD was recorded at 515 nm followed by construction of a plot between inhibition (%) and concentration to calculate IC₅₀ value (concentration that scavenged 50% of the free radical).

Estimation of Reducing Power

The tested EOs were assessed for their reducing ability using following the method used by Abbas et al.¹⁷ Different amounts of EOs ranging from 2.5 mg to 10 mg were reacted with Na phosphate buffer (.2 M, pH = 6.6, 5.0 mL) and potassium ferricyanide (1.0%, 5 mL). After incubating at 50°C, this mixture was mixed with trichloroacetic acid (10%, 5.0 mL) followed by centrifuging for 10 minutes to separate upper layer which was diluted with water (5.0 mL). The resultant solution was added to iron (III) chloride (.1%, 1.0 mL) to record its optical density (OD) at 700 nm.

Antimicrobial Potential of EOs

Antimicrobial activity of tested EOs was evaluated against different pathogenic strains of bacteria (S. aureus, P. multocida, E coli, and B subtilis) and fungus (A. flavus, A niger, A alternate, and G lucidum,) were used. Nutrient agar (NA) at temperature of 37°C was used as culture medium to grow bacteria while potato dextrose agar (PDA) at 30°C was used for fungal growth. The growth medium was purchased from Oxoid (Hampshire, UK). Streptomycin was used as standard drug for bacterial strains while fluconazol was selected as standard drug for fungus.

Inhibition zone of the tested EO was measured by the disc diffusion method²⁸ by considering 6 mm of sample-soaked filter paper discs and placed on the already incubated culture of bacteria and fungus. The corresponding standard drugs were named as positive control; however, filter paper disc without sample was named as negative control. The inhibition zone was calculated after 24 hours of incubation for bacterial strains and 3 days of incubation for fungal strains.

Furthermore, to calculate the minimum inhibitory concentration of EO that inhibited the growth of the microorganism, micro dilution broth method previously used by Abbas et al¹⁷ was selected. The MIC (minimum inhibitory concentration) value (mg/mL) gives a good knowledge regarding the antimicrobial activity of the tested EO, smaller the MIC value greater is the antimicrobial activity.

Biofilm Inhibition

The biofilm inhibition of EOs was checked against E coli and S aureus following a modified method reported by Regev-Shoshani et al.²⁹ The cell suspension of microorganism (100 μL) was added into 96-well micro titre plate along with the EO sample. After incubation for 3 days at 37°C, the liquid was removed and 100 μL of crystal violet solution (1%) was added. After staining for 30 minutes, dye was removed, and wells were washed. It was incubated again for 15 minutes after adding rectified ethanol (95%) and then the absorbance was noted at 570 nm. Positive control (rifamacin) was also processed under similar experimental conditions.

Hemolytic Activity

The assay of hemolytic activity was performed in vitro on human erythrocytes of O blood group reported by Malagoli.³⁰ Blood sample was obtained from volunteers, centrifuged for 5 minutes at 5000 rpm, and 2.0% suspension of erythrocyte was made in phosphate buffer saline. In this process, .85% of NaCl solution was added into various concentration of the EO solutions (50–500 μg/mL), followed by the addition of already prepared erythrocyte suspension, which was kept at room temperature for 30 minute, upper layer of the mixture was collected to record the OD at 540 nm. Positive control (triton X-100) and negative control (phosphate buffer saline) were processed under similar experimental conditions. Hemolytic activity (%) was estimated by comparing with the absorbance value of positive control.³¹

Statistical Analysis

Three different samples of coriander plant leaves/EOs were used in each extraction technique to perform replicate experiments. To evaluate significant difference (P < .05), ANOVA (analysis of variance) was conducted by STATISTICA v5.5 (Computer software). The data were computed as mean ± standard deviation.³²

Results and Discussion

Yield and Physico-chemical Analysis

The results for percentage yield and physical parameters of the tested coriander EO are given in Table 1. The EO yield (w/w) (.12%) by hydro-distillation is slightly higher than that of essential oil yield by SCFE technique (.09%), though this difference is not significant. This low yield of coriander essential oil in our research work is quite comparable to that of a previous study³⁷ in which the yield was noted to be .15%. Furthermore, the color of EO obtained by SCFE was light yellow while by hydro-distillation was just colorless. The test of solubility revealed that the oil obtained by hydro-distillation has slightly higher solubility (2.5 volume in 70% alcohol) than its counterpart (2.1 volume in 70% alcohol). Similarly, the oil extracted by SCFE has slightly higher density (.87 g/mL at 25°C) and refractive index (1.3800) than the oil obtained by hydro-distillation, which offered showed a density of .84 g/mL at 25°C and refractive index of 1.3300. It is clear from the results of physical analysis that the oils obtained by both the techniques are not significantly different from each other, except the oil obtained by SCFE is slightly concentrated. The minute difference in the physico-chemical parameters of the EOs in relation to the techniques employed can be connected with variation in the EO’s composition.³³ In the previous studies, it is also appraised that the EO obtained through multiple methods have a little difference in the physico-chemical properties.^34,35

Table 1.

Yield (%) and physico-chemical parameters of coriander EOs.

Technique	Yield (w/w) (%)	Color	Solubility	Density g/mL (25^oC)	Refractive index (25^oC)
SCFE*	.09 ± .01^a	Very light yellow	2.1 part in alcohol (70%)	.87 ± .03^a	1.38 ± .04^a
Hydro-distillation	.12 ± .02^a	Just colorless	2.5 part in alcohol (70%)	.84 ± .02^a	1.33 ± .05^a

Note: Data is reported as mean ± SD, superscript (a) within the same column show significant (P < .05) differences of means between the two essential oil extraction methods.

*SCFE = Supercritical fluid extraction.

Composition of Coriander Essential Oil

The chemical compositional data of coriander EO produced by SCFE and HD and scrutinized by GC-MS is presented in Table 2. In the EO extracted by SCFE, a total 23 different components were identified including the major component linalool (51.34%) and others such as phytol (12.71%), α-pinene (9.91%), methyl linolenate (6.19%), geranyl acetate (4.23%), and camphor (3.45%) (Figure 1). However, in the hydro-distilled essential oil, only 18 components were identified including the principal component such as linalool (61.78%) followed by α-pinene (8.89%), camphor (7.16%), geranyl acetate (5.87%), and nerol (3.15%). The contents of the major components of the tested C. sativum EOs in the present work were quite comparable with those reported earlier.³⁶ Anwar et al.³⁷ found that coriander EO contained linalool (69.60%) as a major component with considerable amount of geranyl acetate (4.99%), γ-terpinene (4.17%), and α-pinene (1.63%). In another study, Singh et al.³ identified total of 52 components from coriander seed EO grown in India. The results revealed that the composition of major components in this study was quite comparable to our present compositional data. In a report by Zoubiri and Baaliouame,³⁸ a total of 17 constituents were recognized in the coriander seed EO from Algeria and linalool was found to be the principal component with the percentage of 73.1%. According to the reports of Bhuiyan et al,³⁹ 53 components were identified from the EO of coriander seeds, harvested in Bangladesh. Again, linalool was found as the chief component followed by geranyl acetate and gamma-terpinene as the second and third larger compounds. Such variation in the chemicals profiling of C. sativum EO may be mainly caused due to different agro-climatic conditions of the harvesting regions which determine and shape the morphological features of the crop.

Table 2.

Percentage composition comparison of some major component in coriander EOs produced by SCFE and HD techniques.

Sr. #	Compound name	IUPAC name	RI*	SCFE**	Hydro-distillation	Method of identification
1	α-Pinene	2,6,6-trimethylbicyclo[3.1.1]hept-2-ene	941	9.91 ± .62^a	8.89 ± .45^b	^a, ^b, ^c
2	Camphene	2,2-dimethyl-3-methylidenebicyclo[2.2.1]heptane	950	—	1.42 ± .07	^a, ^b, ^c
3	o-Cymene	1-methyl-2-(propan-2-yl)benzene	1025	—	2.12 ± .09	^a, ^b, ^c
4	Limonene	1-methyl-4-(1-methylethenyl)-cyclohexene	1035	1.43 ± .12^b	2.49 ± .12^a	^a, ^b, ^c
5	γ-Terpinen	1-methyl-4-propan-2-ylcyclohexa-1,4-diene	1058	—	3.95 ± .21	^a, ^b, ^c
6	Linalool	3,7-dimethylocta-1,6-dien-3-ol	1096	51.34 ± 1.91^b	61.78 ± 3.21^a	^a, ^b, ^c
7	Camphor	1,7,7-trimethylbicyclo[2.2.1]heptan-2-one	1147	3.45 ± .22^b	7.16 ± .34^a	^a, ^b, ^c
8	Nerol	(2Z)-3,7-dimethylocta-2,6-dien-1-ol	1325	—	3.15 ± .19	^a, ^b, ^c
9	Geranyl acetate	[(2E)-3,7-dimethylocta-2,6-dienyl] acetate	1382	4.23 ± .31^b	5.87 ± .31^a	^a, ^b, ^c
10	Methyl Linolenate	methyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate	2040	6.19 ± .62	—	^a, ^b, ^c
11	Linoliec acid	(9Z,12Z)-octadeca-9,12-dienoic acid	2104	1.42 ± .21	—	^a, ^b, ^c
12	Phytol	(E,7R,11 R)-3,7,11,15-tetramethylhexadec-2-en-1-ol	2128	12.71 ± .85	—	^a, ^b, ^c
	Total Components Identified		23		18

Note: Data is reported as mean ± SD, superscript within the same row displays a significant (P< .05) differences of means between SCFE and HD.

*RI = Retention index; **SCFE = Supercritical fluid extraction.

^a = Identification by retention index.

^b = identification by comparing with authentic compounds.

^c = identification by comparing of mass spectra.

Figure 1.

Structure of some major components of coriander essential oil

Antioxidant Potential

Antioxidant potential of the tested coriander EOs obtained by both the techniques was appraised by reducing capacity and free radical scavenging ability (Table 3). The results showed significant difference in the DPPH radical scavenging activity of the EO for both the techniques. The DPPH radical scavenging activity was presented in terms of IC₅₀ value in μg/mL, which showed that the SCF extracted EO has good DPPH radical scavenging activity with least IC₅₀ value (9.11 μg/mL) as compared to hydro-distilled essential oil, which gave IC₅₀ of 10.34 μg/mL. Interestingly, SCF extracted EO gave even better result as compared to the standard compound (BHT) used in the experiment.

Table 3.

Antioxidant potential of coriander EOs.

ExtractionTechnique	DPPH* Radical Scavenging Assay		Reducing Power (Absorbance values)
	DPPH* Radical Scavenging Assay		Concentration (mg/mL)
	Inhibition (%)	IC₅₀, μg/mL	2.5	5.0	7.5	10.0
SCFE**	42 ± 2^b	9.11 ± .21^b	.57 ± .04^b	.83 ± .07^a	1.01 ± .09^a	1.32 ± .12^b
Hydro-distillation	49 ± 2^a	10.34 ± .32^a	.68 ± .08^a	.79 ± .04^a	.98 ± .08^a	1.20 ± .14^b
BHT***	—	10.21 ± .29^a	—	—	—	1.91 ± .21^a

Note: Data is reported as mean ± SD, superscript (a, b) within the same column show significant (P < .05) difference in values for SCFE and HD.

*DPPH = 2,2-diphenyl-1-picrylhydrazyl. **SCFE = Supercritical fluid extraction. ***BHT = Butylated hydroxy toluene.

In reducing power assay, the yellow color of ferric ions is changed into bluish green color of ferrous ions after reduction. The reducing potential of EO depends upon the color intensity of the final reaction mixture, which shows that there is a direct relationship between the color intensity and the antioxidant powder.¹⁷ A gradual increment in the absorbance was recorded with increasing concentration of sample. At the concentration of 10.0 mg/mL, SCF-extracted EO showed better reducing potential (1.32) as compared to hydro-distilled EO (1.20) but less than standard synthetic antioxidant compound, which showed the absorbance of 1.91 at concentration of 10.0 mg/mL.

Foudah et al⁴⁰ reported in his research work that coriander essential oil is a promising reservoir of natural antioxidants. In another study,⁴¹ the antioxidant potential was determined in oil and extracts from seeds and leaves of coriander. Overall, from the results of antioxidant activity it can be concluded that EO obtained from SCFE has better antioxidant and nutra-pharmaceutical potential as compared to EO obtained by hydro-distillation, which reveals the superiority of SCFE techniques over traditionally used hydro-distillation technique.

Antimicrobial Activity

Antimicrobial activity of coriander EO was determined and the related results are presented in Table 4. The coriander EO extracted by SCFE showed maximum antibacterial activity (12 mm inhibition zone) against P. multocida with MIC (115 μg/mL) while the hydro-distilled EO had potent activity (21 mm inhibition zone) against E coli with MIC = 72 μg/mL. On the other hand, SCF extracted EO also showed stronger antifungal activity (20 mm inhibition zone) against A alternata with MIC = 82 μg/mL while the hydro-distilled EO was more resistant against A niger with inhibition zone and MIC values of 19 mm and 74 μg/mL, respectively. However, in comparison with standard drugs, antimicrobial potency of EOs extracted either by SCFE or hydro-distillation possessed less antimicrobial activity than the standard compounds such as streptomycin and fluconazole that were used as antibacterial and antifungal drugs in the present experiments, respectively. Interestingly, in contrast to the observations of Cantore et al,⁴² the tested EOs were more resistant to gram negative bacteria such as E coli then Gram positive bacteria. Such types of trends in antimicrobial potential might be ascribed to chemical composition, agro-ecological, agro-climatic conditions, and analytical control of the experimental assays.

Table 4.

Antimicrobial potential of coriander EOs.

Microorganism	Inhibition zone (mm)			MIC (μg/mL)
Microorganism	SCFE*	Hydro distillation	Standard Drug ^**	SCFE	Hydro distillation	Standard Drug ^**
Bacteria
S. aureus	9.00 ± .25 ^c	16.00 ± .42^b	28.00 ± 1.12^a	132 ± 5^a	129 ± 5^a	62 ± 2^b
B. subtilis	9.00 ± .21^c	17.00 ± .37^b	31.00 ± 1.18^a	126 ± 4^a	103 ± 3^b	73 ± 3^c
P. multocida	12.00 ± .27^c	18.00 ± .51^b	30.00 ± 1.12^a	115 ± 3^a	86 ± 2^b	54 ± 2^c
E. coli	11.00 ± .22^c	21.00 ± .53^b	29.00 ± 1.21^a	127 ± 4^a	72 ± 2^b	63 ± 3^c
Fungus
G. lucidum	15.00 ± .52 ^c	17.00 ± .61^b	26.00 ± 1.08^a	93 ± 2^a	82 ± 2^b	73 ± 2^c
A. flavus	18.00 ± .68^b	15.00 ± .58^c	28.00 ± 1.15^a	86 ± 3^b	102 ± 4^a	78 ± 2^c
A. niger	14.00 ± .47^c	19.00 ± .57^b	30.00 ± 1.23^a	82 ± 2^a	74 ± 2^b	53 ± 2^c
A. alternata	20.00 ± .72^b	17.00 ± .67^c	24.00 ± 1.02^a	82 ± 2^b	92 ± 3^a	83 ± 3^b

Note: Data is reported as mean ± SD, superscript (a, b, c) within the same row show significant (P < .05) differences of means between the two essential oil extraction methods. ** Standard drug Streptomycin for bacteria while Fluconazole for fungus.

*SCFE = Supercritical fluid extraction.

Several essential oils, which are mainly composed of terpenoids, were also studied for the occurrence of phenolic antimicrobial components.^15,17,43-45 The phenolic compounds present in the plant essential oils show higher antimicrobial effect as compared to aldehyde, alcohol, and ketones.^46,47

Other Bioactivities (Biofilm Inhibition and Hemolytic Activity)

To further elucidate biological attributes of tested coriander EOs, some other biological activities like biofilm inhibition and hemolytic activity was also measured. Biofilm can preserve the bacteria by hindering the effects of antibiotics and antioxidants and host immune responses.⁴⁸ Due to the presence of bacterial biofilm and its relative permeability some antibiotics and antioxidants would become ineffective,^49-54 so a good antioxidant agent must also possess biofilm inhibition activity. Data regarding the biofilm inhibition is given in Table 5, which shows that the maximum biofilm inhibition was given by SCF extracted EO against S aureus (78.61%) but less than Rifamacin (87.43%), standard drug used in the experiment. The result of our research work is in line with those of Duarte et al,⁵⁵ who described that coriander EO displayed significant anti-biofilm potential.

Table 5.

Bioactivities of coriander EOs.

Bioactivity	Microbe	Coriander EO		Rifamacin	Triton-x-100
Bioactivity	Microbe	SCFE*	Hydro-distillation	Rifamacin	Triton-x-100
Biofilm Inhibition %	S. aureus	78.61 ± 2.62^b	52.92 ± 1.82^c	87.43 ± 3.89^a	—
Biofilm Inhibition %	E. coli	37.96 ± 1.26^c	64.75 ± 1.92^b	88.92 ± 3.45^a	—
Hemolytic Assay %		4.86 ± .19^b	31.58 ± 1.92^a	—	100

Note: Data is reported as mean ± SD, superscript (a, b, c) within the same row show significant (P < .05) differences of means between the two essential oil extraction methods.

*SCFE = Supercritical fluid extraction.

It is observed that some antioxidant compounds may have good antioxidant activity but also possess hemolytic effect, due to this reason they may not be able to use in pharmaceutical preparation. Hence for a good and safer antioxidant, it must have least hemolytic attributes. Therefore, in this project, hemolytic activity of tested EO was assessed (Table 5). The results clearly showed that the EO obtained by SCFE method has far less hemolytic activity (4.86%) as compared to the EO extracted by hydro-distillation (31.58%). These findings also support that the SCFE technique is better than conventional hydro-distillation technique toward isolation of nutra-pharmaceutical grade EOs. There are no previous literature reports available with which we can compare our results of the hemolytic activity. Hence, the present investigation revealed that the coriander EO is safer since no toxicity was observed, which have potential application for the development of nutra-pharmaceutical agents. The plants produced bioactive agents and these bioactive agents can be used for different fields for practical applications.^56-58

Conclusion

Although the yield and physico-chemical attributes of the coriander EOs tested were significantly varied for SCFE and HD techniques. However, the results revealed that the coriander EO obtained from both the techniques have significant variations with regard to the biological activities. The EO obtained by SCFE technique possesses better biological potential due to presence of higher quantity of different potent bioactive components in comparison to EO recovered by hydro-distillation. Thus, in terms of their nutra-pharmaceutical applications, SCFE can be considered to be superior extraction techniques for the recovery of biologically active volatile components. These research findings might motivate more researchers to explore the essential oil and phytochemicals profiling and biological potential of other medicinal plants from Soon Valley, especially meeting the demand of local modern food and pharmaceutical industry. Such data might also encourage the local growers and community to cultivate and harvest such medicinal herbs on large scale and install on farm SCFE plants for isolation of valuable essential oils with multiple food and nutra-pharmaceutical applications.

Footnotes

Acknowledgments

The authors express their gratitude to Princess Nourah bint Abdulrahman University Researchers Supporting Project (Grant No. PNURSP2022R158), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: Princess Nourah bint Abdulrahman University Researchers Supporting Project number (PNURSP2022R158) Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.

ORCID iDs

Naveed Ahmad

Munawar Iqbal

References

Weisany

Tahir

NAR

Schenk

. Coriander/soybean intercropping and mycorrhizae application lead to overyielding and changes in essential oil profiles. Eur J Agron. 2021;126:126283. DOI: 10.1016/j.eja.2021.126283.

Sahib

Anwar

HGilani

Hamid

Saari

Alkharfy

. Coriander (Coriandrum sativum L.): A potential source of high-value components for functional foods and nutraceuticals. A review. Phytother. Res. 2013;27(10):1439-1456. DOI: 10.1002/ptr.4897.

Singh

Maurya

De Lampasona

Catalan

. Studies on essential oils, Part 41. Chemical composition, antifungal, antioxidant and sprout suppressant activities of coriander (Coriandrum sativum) essential oil and its oleoresin. Flavour Fragrance J. 2006;21(3):472-479. DOI: 10.1002/ffj.1608.

Sriti

Wannes

Talou

Mhamdi

Cerny

Marzouk

. Lipid profiles of Tunisian coriander (Coriandrum sativum) seed. JAOCS (J Am Oil Chem Soc). 2010;87(4):395-400. DOI: 10.1007/s11746-009-1505-1.

Peter

Babu

. Introduction to herbs and spices: medicinal uses and sustainable production. In: Handbook of herbs and spices. Woodhead Publishing; 2012:1-16. DOI: 10.1533/9780857095688.1.

Ceska

Chaudhary

Warrington

Ashwood-Smith

Bushnell

Poultont

. Coriandrin, a novel highly photoactive compound isolated from Coriandrum sativum. Phytochemistry (Oxf). 1988;27(7):2083-2087. DOI: 10.1016/0031-9422(88)80101-8.

Pellegrini

Rossi

Palmieri

, et al. Salmonella enterica control in stick carrots through incorporation of coriander seeds essential oil in sustainable washing treatments. Front Sustain Food Syst. 2020;4:14. DOI: 10.3389/fsufs.2020.00014.

Kamat

Pingulkar

Bhushan

Gholap

Thomas

. Potential application of low dose gamma irradiation to improve the microbiological safety of fresh coriander leaves. Food Control. 2003;14(8):529-537. DOI: 10.1016/S0956-7135(02)00116-0.

Burdock

Carabin

. Safety assessment of coriander (Coriandrum sativum L.) essential oil as a food ingredient. Food Chem Toxicol. 2009;47(1):22-34. DOI: 10.1016/j.fct.2008.11.006.

10.

Prachayasittikul

Ruchirawat

Prachayasittikul

. Coriander (Coriandrum sativum): A promising functional food toward the well-being. Food Res Int. 2018;105:305-323. DOI: 10.1016/j.foodres.2017.11.019.

11.

Kačániová

Galovičová

Ivanišová

Vukovic

Štefániková

Valková

, et al. Antioxidant, antimicrobial and antibiofilm activity of coriander (Coriandrum sativum L.) essential oil for its application in foods. Foods. 2020;9(3):282. DOI: 10.3390/foods9030282.

12.

Begnami

Duarte

MCT

Furletti

Rehder

VLG

. Antimicrobial potential of Coriandrum sativum L. against different Candida species in vitro. Food Chem. 2010;118(1):74-77. DOI: 10.1016/j.foodchem.2009.04.089.

13.

Maksimovic

Stojanović

Šoštarić

Dajić

Ristić

. Composition and radical‐scavenging activity of Thymus glabrescens Willd (Lamiaceae) essential oil. J Sci Food Agric. 2008;88(11):2036-2041. DOI: 10.1002/jsfa.3311.

14.

Busatta

Vidal

Popiolski

, et al. Application of Origanum majorana L. essential oil as an antimicrobial agent in sausage. Food Microbiol. 2008;25(1):207-211. DOI: 10.1016/j.fm.2007.07.003.

15.

Anwar

Ali

Hussain

Shahid

. Antioxidant and antimicrobial activities of EO and extracts of fennel (Foeniculum vulgare Mill.) seeds from Pakistan. Flavour Fragrance J. 2009;24(4):170-176. DOI: 10.1002/ffj.1929.

16.

Hussain

Anwar

Sherazi

STH

Przybylski

. Chemical composition, antioxidant and antimicrobial activities of basil (Ocimum basilicum) essential oils depends on seasonal variations. Food Chem. 2008;108(3):986-995. DOI: 10.1016/j.foodchem.2007.12.010.

17.

Abbas

Anwar

Ahmad

. Variation in physico-chemical composition and biological attributes of common basil essential oils produced by hydro-distillation and super critical fluid extraction. J. Essent. Oil-Bear. Plants. 2017;20(1):95-109. DOI: 10.1080/0972060X.2017.1280418.

18.

Hussain

Anwar

Chatha

, et al. Chemical composition and bioactivity studies of the essential oils from two Thymus species from the Pakistani flora. LWT--Food Sci Technol. 2013;50(1):185-192. DOI: 10.1016/j.lwt.2012.06.003.

19.

Kapustová

Granata

Napoli

, et al. Nanoencapsulated Essential Oils with Enhanced Antifungal Activity for Potential Application on Agri-Food, Material and Environmental Fields. Antibiotics. 2021;10(1):31. DOI: 10.3390/antibiotics10010031.

20.

Van Vuuren

Viljoen

Őzek

Demirci

Başer

KHC

. Seasonal and geographical variation of Heteropyxis natalensis essential oil and the effect thereof on the antimicrobial activity. South Afr J Bot. 2007;73(3):441-448. DOI: 10.1016/j.sajb.2007.03.010.

21.

Masotti

Juteau

Bessière

Viano

. Seasonal and phenological variations of the essential oil from the narrow endemic species Artemisia molinieri and its biological activities. J Agric Food Chem. 2003;51(24):7115-7121. DOI: 10.1021/jf034621y.

22.

Sokolova

Orav

Koel

Kailas

Müürisepp

. Composition of the oil and supercritical fluid CO2 extract of sweet gale (Myrica gale L.) fruits. J Essent Oil Res. 2005;17(2):188-191. DOI: 10.1080/10412905.2005.9698871.

23.

Younis

Mehdi

Riaz

. Supercritical carbon dioxide extraction and gas chromatography analysis of Jasminum sambac essential oil. Pakistan J Bot. 2011;43:163-168. http://pakbs.org/pjbot/PDFs/43(SI)/24.pdf

24.

Wood

Bernards

Wan

Charpentier

. Extraction of ginsenosides from North American ginseng using modified supercritical carbon dioxide. J Supercrit Fluids. 2006;39(1):40-47. DOI: 10.1016/j.supflu.2006.01.016.

25.

Ahmad

Zuo

Anwar

Hameed

. Characterization of free and conjugated phenolic compounds in fruits of selected wild plants. Food Chem. 2016;190:80-89. DOI: 10.1016/j.foodchem.2015.05.077.

26.

Rezazadeh

Baha-Aldini

BZBF

Vatanara

, et al. Comparison of super critical fluid extraction and hydrodistillation methods on lavander’s essential oil composition and yield. J. Med. Plants. 2008;7(25):63-68. http://dorl.net/dor/20.1001.1.2717204.2008.7.25.23.9

27.

Graikou

KVK

Ngassapa

Runyoro

Chinou

. Composition and antimicrobial activity of the essential oils of three Satureja species growing in Tanzania. Food Chem. 2007;103(2):319-324. DOI: 10.1016/j.foodchem.2006.07.051.

28.

Wayne

. NCCLS: National Committee for Clinical Laboratory Standards. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; 1999.

29.

Regev-Shoshani

Miller

Av-Gay

. Slow release of nitric oxide from charged catheters and its effect on biofilm formation by. Escherichia coliAntimicrob. Agents Chemother. 2010;54(1):273-279. DOI: 10.1128/AAC.00511-09.

30.

Malagoli

. A full-length protocol to test hemolytic activity of palytoxin on human erythrocytes. Invertebr Surviv J. 2007;4(2):92-94. http://hdl.handle.net/11380/421488

31.

Gould

Lansley

Brown

Forbes

Martin

. Mitigation of surfactant erythrocyte toxicity by egg phosphatidylcholine. J Pharm Pharmacol. 2000;52(10):1203-1209. DOI: 10.1211/0022357001777333.

32.

Steel

Torrie

. Principles and Procedures of Statistics. New York, NY: McGraw-Hill Book Co. Inc.; 1980:481.

33.

Vijayalakshmi

Tripathi

Ravichandiran

. Characterization and evaluation of antidermatophytic activity of the essential oil from Artemisia nilagirica leaves growing wild in nilgiris. Int J Pharm Pharmaceut Sci. 2010;2:93-97. https://innovareacademics.in/journal/ijpps/Vol2Suppl4/803.pdf

34.

Hassanein

El-Gendy

AENG

Saleh

Hendawy

Elmissiry

Omer

. Profiling of essential oil chemical composition of some Lamiaceae species extracted using conventional and microwave‐assisted hydrodistillation extraction methods via chemometrics tools. Flavour Fragrance J. 2020;35(3):329-340. DOI: 10.1002/ffj.3566.

35.

Turek

Stintzing

. Stability of essential oils: a review. Compr Rev Food Sci Food Saf. 2013;12(1):40-53. DOI: 10.1111/1541-4337.12006.

36.

Wahba

Abd Rabbu

Ibrahim

. Evaluation of essential oil isolated from dry coriander seeds and recycling of the plant waste under different storage conditions. Bull Natl Res Cent. 2020;44(1):1-7. DOI: 10.1186/s42269-020-00448-z.

37.

Anwar

Sulman

Hussain

Saari

Iqbal

Rashid

. Physicochemical composition of hydro-distilled essential oil from coriander (Coriandrum sativum L.) seeds cultivated in Pakistan. J Med Plants Res. 2011;5(15):3537-3544. DOI: 10.5897/JMPR.9000978.

38.

Zoubiri

Baaliouamer

. Essential oil composition of Coriandrum sativum seed cultivated in Algeria as food grains protectant. Food Chem. 2010;122(4):1226-1228. DOI: 10.1016/j.foodchem.2010.03.119.

39.

Bhuiyan

MNI

Begum

Sultana

. Chemical composition of leaf and seed essential oil of Coriandrum sativum L. from Bangladesh. Bangladesh J Pharmacol. 2009;4(2):150-153. doi:10.3329/bjp.v4i2.2800.

40.

Foudah

Alqarni

Alam

Salkini

Ahmed

EOI

Yusufoglu

. Evaluation of the composition and in vitro antimicrobial, antioxidant, and anti-inflammatory activities of Cilantro (Coriandrum sativum L. leaves) cultivated in Saudi Arabia (Al-Kharj). Saudi J Biol Sci. 2021;28(6):3461-3468. DOI: 10.1016/j.sjbs.2021.03.011.

41.

Wangensteen

Samuelsen

Malterud

. Antioxidant activity in extracts from coriander. Food Chem. 2004;88(2):293-297. DOI: 10.1016/j.foodchem.2004.01.047.

42.

Lo Cantore

Iacobellis

De Marco

Capasso

Senatore

. Antibacterial activity of Coriandrum sativum L. and Foeniculum vulgare Miller var. vulgare (Miller) essential oils. J Agric Food Chem. 2004;52(26):7862-7866. DOI: 10.1021/jf0493122.

43.

Abebe

Mekonnen

. Assessment of antioxidant and antibacterial activities of crude extracts of Verbena officinalis linn root or atuch (Amharic). Chem Int. 2017;3:172-184.

44.

Rauf

Hassan

Rafique

. Nutra-pharmaceutical efficacy appraisal of Lavandula stoechas leaves extracts in different solvents. Chem Int. 2021;8(4):242-248.

45.

Friday

Akwada

Igwe

. Phytochemical screening and antimicrobial studies of Afzelia africana and Detarium microcarpum seeds. Chem Int. 2018;4(3):170-176.

46.

Tao

Sedman

Ismail

. Antimicrobial activity of various essential oils and their application in active packaging of frozen vegetable products. Food Chem. 2021;360:129956. DOI: 10.1016/j.foodchem.2021.129956.

47.

Tepe

Daferera

Sokmen

Polissiou

. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chem. 2005;90(3):333-340. DOI: 10.1016/j.foodchem.2003.09.013.

48.

Darouiche

Raad

Heard

, et al. A comparison of two antimicrobial-impregnated central venous catheters. NEJM. 1999;340(1):1-8. doi:10.1056/NEJM199901073400101.

49.

Nuță

Limban

Chiriță

, et al. Contribution of Essential Oils to the Fight against Microbial Biofilms—A Review. Processes. 2021;9(3):537. DOI: 10.3390/pr9030537.

50.

Dolatabadi

Świergosz

Ahmadzadeh

. Electro-Fenton approach in oxidative degradation of dimethyl phthalate-The treatment of aqueous leachate from landfills. Sci Total Environ. 2021;772:145323.

51.

Dolatabadi

Ghaneian

Wang

Ahmadzadeh

. Electro-Fenton approach for highly efficient degradation of the herbicide 2, 4-dichlorophenoxyacetic acid from agricultural wastewater: Process optimization, kinetic and mechanism. J. Mol. Liquids. 2021;334:116116.

52.

Ahmadzadeh

Kassim

Abdollahi

. Conductometric Study of Complexation Reaction Between Meso-octamethylcalix [4] pyrrole with Titanium Cation in. Int J Electrochem Sci. 2011;6:4749-4759.

53.

Ahmadzadeh

Rezayi

Karimi-Maleh

Alias

. Conductometric measurements of complexation study between 4-Isopropylcalix [4] arene and Cr3+ cation in THF–DMSO binary solvents. Measurement. 2015;70:214-224.

54.

Rezayi

Heng

Kassim

Ahmadzadeh

Abdollahi

Jahangirian

. Immobilization of tris (2 pyridyl) methylamine in a PVC-Membrane Sensor and Characterization of the Membrane Properties. Chem Cent J. 2012;6(1):1-6.

55.

Duarte

Ferreira

Oliveira

Domingues

. Effect of coriander oil (Coriandrum sativum) on planktonic and biofilm cells of Acinetobacter baumannii. Nat Prod Commun. 2013;8(5):1934578X1300800532.

56.

Almessiere

Slimani

Rehman

Khan

Sertkol

Baykal

. Green synthesis of Nd substituted Co-Ni nanospinel ferrites: a structural, magnetic, and antibacterial/anticancer investigation. J Phys Appl Phys. 2021;55:055002.

57.

Al-Jameel

Rehman

Almessiere

, et al. Anti-microbial and anti-cancer activities of Mn0.5Zn0.5DyxFe2-xO4 (x ≤ 0.1) nanoparticles. Artif Cell Nanomed Biotechnol;49(2021):493-499.

58.

Almessiere

Slimani

Auwal

, et al. Biosynthesis effect of Moringa oleifera leaf extract on structural and magnetic properties of Zn doped Ca-Mg nano-spinel ferrites. Arab J Chem. 2021;14:103261.

Characterization of Bioactives and Nutra-Pharmaceutical Potential of Supercritical Fluid and Hydro-Distilled Extracted Coriander Leaves Essential Oil

Abstract

Keywords

Introduction

Experimental

Sample Collection

Chemical and Reagents

Recovery of EO

Hydro-Distillation

Supercritical Fluid Extraction (SCFE)

Analysis of Essential Oils

Yield and Physical Analyses

Gas Chromatography (GC)

GC-MS Analysis

Antioxidant Potential

DPPH. Scavenging Assay

Estimation of Reducing Power

Antimicrobial Potential of EOs

Biofilm Inhibition

Hemolytic Activity

Statistical Analysis

Results and Discussion

Yield and Physico-chemical Analysis

Composition of Coriander Essential Oil

Antioxidant Potential

Antimicrobial Activity

Other Bioactivities (Biofilm Inhibition and Hemolytic Activity)

Conclusion

Footnotes

Acknowledgments

Declaration of Conflicting Interests

Funding

ORCID iDs

References

DPPH^. Scavenging Assay