The Best Approach for Laparoscopic Fluorescence Cholangiography: Overview of the Literature and Optimization of Dose and Dosing Time

Abstract

Background: Fluorescence cholangiography using indocyanine green (ICG) can enhance orientation of bile duct anatomy during laparoscopic cholecystectomy. To ensure clear discrimination between bile ducts and liver, the fluorescence ratio between both should be sufficient. This ratio is influenced by the ICG dose and timing of fluorescence imaging. We first systematically identified all strategies for fluorescence cholangiography. Second, we aimed to optimize the dose of ICG and dosing time in a prospective clinical trial. Methods: PubMed was searched for clinical trials studying fluorescence cholangiography. Furthermore, 28 patients planned to undergo laparoscopic cholecystectomy were divided into 7 groups, receiving different intravenous doses (5 or 10 mg ICG) at different time points (0.5, 2, 4, 6, or 24 hours prior to surgery). Results: The systematic review revealed 27 trials including 1057 patients. The majority of studies used 2.5 mg administered within 1 hour before imaging. Imaging 3 to 24 hours after ICG administration was never studied. The clinical trial demonstrated that the highest bile duct-to-liver ratio was achieved 3 to 7 hours after administration of 5 mg and 5 to 25 hours after administration of 10 mg ICG. Up to 3 hours after administration of 5 mg and up to 5 hours after administration of 10 mg ICG, the liver was equally or more fluorescent than the cystic duct, resulting in a ratio ≤1.0. Conclusion: This study shows for the first time that the interval between ICG administration and intraoperative fluorescence cholangiography should be extended. Administering 5 mg ICG at least 3 hours before imaging is easy to implement in everyday clinical practice and results in bile duct-to-liver ratios >1.0.

Keywords

fluorescence cholangiography intraoperative bile duct imaging indocyanine green dose and time optimization fluorescence imaging bile duct anatomy

Introduction

Iatrogenic bile duct injury after laparoscopic cholecystectomy is a rare but serious complication with an incidence ranging from 0.08% to 1.5%.^1-3 Bile duct injuries are mostly the result of misidentification of the common bile duct (CBD) or the common hepatic duct (CHD) for the cystic duct (CD). Transection of the CBD or CHD leads to severe complications, long-term mortality rates up to 21%, and significant costs.⁴ Important contributing factors are the learning curve associated with laparoscopic surgery and the presence of (misinterpreted) aberrant bile duct anatomy. Because laparoscopic cholecystectomy is currently one of the most performed surgeries worldwide, safer approaches are desirable. Use of the critical view of safety—a method of intraoperative identification of the CD and cystic artery—has been developed to minimize the risk of bile duct injury.⁵ Although this strategy is now implemented worldwide, bile duct injuries still occur.

Identification of bile ducts during laparoscopic cholecystectomies can be enhanced by intraoperative cholangiography (IOC). This imaging technique requires injection of radio-opaque contrast into the CD, whereupon a series of X-rays are taken. IOC can accurately depict biliary anatomy and some advocate that it should be performed routinely.^6,7 However, this technique is expensive, time-consuming, and exposes patients and health care personnel to radiation. Moreover, bile duct cannulation may cause bile duct injury itself and images can be difficult to interpret.^8,9

An alternative intraoperative imaging technique that is less invasive, does not require radiation, and is not time consuming is near-infrared (NIR) fluorescence cholangiography. Feasibility of real-time NIR fluorescence imaging of bile ducts using indocyanine green (ICG) was first described in 2008.¹⁰ After intravenous administration, ICG is cleared via the liver, enabling visualization of liver tumors and bile duct anatomy.^11-13 ICG has a favorable safety profile; side effects are reported in less than 1 out of 40 000 patients.¹⁴

Several studies have described fluorescence cholangiography during open, laparoscopic, and robotic cholecystectomy.^15-19 However, only few tried to optimize the dose and timing of administration.^15,20 This is important, because if fluorescence imaging is performed directly after administration of ICG, the liver will be highly fluorescent while bile ducts do not yet contain ICG. Optimal would be a high fluorescence signal in bile ducts, while liver tissue in the background stains dark. Excretion of ICG into the bile reaches a peak approximately 2 hours after dosing and remains detectable long after injection.²¹ Yet most studies inject ICG within the first hour prior to imaging. It is already shown that discrimination between bile ducts and liver is enhanced when the time interval between ICG injection and fluorescence imaging is prolonged to 24 hours instead of 30 minutes.¹⁵ Additionally, a recent study showed that a dose of 0.25 mg/kg, administered at least 45 minutes prior to fluorescence imaging, facilitates accurate bile duct identification.²⁰ Although several ICG doses (up to 0.25 mg/kg) were tested in this study, fluorescence cholangiography was performed only up to 180 minutes after ICG administration. The optimal dose for laparoscopic fluorescence cholangiography after an extended interval between dosing and imaging as determined by Verbeek et al,¹⁵ i.e. 10 mg ICG, was not studied. Moreover, dosing times between 3 and 6 hours prior to surgery have never been studied, while these are clinically relevant.

In the current study, we first performed a systematic literature research to identify all used strategies for fluorescence cholangiography in clinical studies. Second, we optimized the dose of ICG and dosing time, ranging from 0.5 to 24 hours prior to surgery, to distinguish bile ducts from adjacent liver tissue.

Materials and Methods

Systematic Review of the Literature

We systematically searched PubMed for clinical trials about fluorescence cholangiography performed during open, laparoscopic, or robotic surgery since 2008. Only articles written in English and describing 3 or more patients were included. Selection of relevant titles was performed independently by 2 reviewers. Subsequently, all included articles were scanned on the content of the abstract, again by 2 independent reviewers.

Clinical Trial

This study was approved by the Medical Ethics Committee of the Leiden University Medical Center and performed in accordance with the ethical standards of the Helsinki Declaration of 1975 (Netherlands Trial Registry: NTR5623). Twenty-eight patients suffering from cholecystolithiasis and/or cholecystitis, and planned for laparoscopic cholecystectomy, were included. Exclusion criteria were based on contraindications for ICG: renal impairment (estimated glomerular filtration rate <55); allergy for iodine, shellfish, or ICG; hyperthyroidism and pregnancy. All patients provided informed consent.

Patients were first assigned into 2 dose groups receiving either 5 mg or 10 mg ICG. Patients assigned to the 5 mg group were planned to receive their ICG dose 30 minutes, 2, 4, or 6 hours prior to surgery (4 patients per time interval). Patients assigned to the 10 mg group were planned to receive their ICG dose 4, 6, or 24 hours prior to surgery. Patients were distributed over the different groups based on their order in the surgical program. Patients scheduled early in the morning were allocated in the brief interval groups, while patients late in the afternoon were allocated into the longer interval groups. Patients were selected for the 24hour interval group if their procedure was scheduled on Tuesday until Friday. Standard-of-care laparoscopic cholecystectomy was performed. When the surgeon considered it to be necessary, IOC was performed. Fluorescence imaging of bile ducts and adjacent structures was performed at least 3 times before the critical view of safety was assessed.

Intraoperative Near-Infrared Fluorescence Imaging System

Laparoscopic imaging was performed using a high-definition (HD) fluorescence laparoscope (Karl Storz Endoscopes, Germany) through a standard 12 mm subumbilical trocar port. The system was equipped with a visible and 760 nm (ICG modus) light source. No overlay of visible and ICG images was possible, but anatomical orientation could be maintained due to easy and fast switching between both channels using a foot pedal.

Preparation and Administration of Indocyanine Green

Twenty-five mg ICG (Pulsion Medical Systems, Munich, Germany) was resuspended in 10 cc sterile water to yield a concentration of 2.5 mg/mL. To obtain doses of 5 mg and 10 mg ICG, respectively, 2 and 4 mL of the solution was intravenously administered.

Postoperative Questionnaire for Surgeons

After surgery, the surgeon completed a questionnaire to score the following statements about fluorescence imaging: (a) it could decrease the number of bile duct complications; (b) it led to better detection of bile ducts; (c) the technique was of added value for this procedure; (d) it needs to be standard-of-care during laparoscopic cholecystectomy; and (e) it delays surgery. Surgeons scored these statements in the following range: totally disagree, somewhat disagree, neutral, somewhat agree, and totally agree.

Data Analysis

Data were analyzed using GraphPad Prism Software (Version 5.01, La Jolla, CA). Bile duct-to-liver ratios (BLR) were calculated by dividing the fluorescence signal of the CD by the fluorescence signal of the liver. Intraoperative fluorescence images of 3 different time points were analyzed using Image J (version 1.49b, National Institutes of Health, Bethesda, MD).

Results

Systematic Review

A total of 242 articles were identified (Figure 1). After reviewing all titles and subsequently, content of the abstracts, 27 eligible clinical studies were reviewed (Table 1).^{10,15-20,22-42} Used ICG doses ranged from 0.025 mg in total to 0.25 mg/kg. Most studies (17/27) used 2.5 mg administered within 1 hour prior to fluorescence imaging. Timing of ICG administration differed from 0 (intraoperatively) up to 24 hours. No clinical trial studied fluorescence cholangiography 3 to 24 hours after ICG administration. The majority of studies (24/27) performed ICG injection 30 to 60 minutes prior to surgery. Successful identification of the CD by fluorescence imaging was achieved in 98% (range: 48% to 100%) of all included patients (n = 1057). Two studies investigated fluorescence cholangiography using different doses of ICG and time of administration.^15,20 They studied the doses 5 or 10 mg and 0.025 or 0.25 mg/kg, and time points of 10 to 180 minutes and 30 minutes or 24 hours prior to surgery.

Figure 1.

Flowchart of the literature search.

Table 1.

Relevant Articles About Fluorescence Cholangiography.

Study	Year	n	Procedure	Fluorescence Imaging System	Dose of ICG (mg)	Timing of Injection	IR of CD (%)
Mitsuhashi et al¹⁰	2008	5	OC	Photo Dynamic Eye	2.5 mg	30 minutes prior to surgery	5/5 (100%)
Ishizawa et al²²	2009	10	OC	Photo Dynamic Eye	2.5 mg	60 minutes prior to surgery/during conversion	10/10 (100%)
Aoki et al²³	2010	14	LC	Hamamatsu laparoscope	12.5 mg	30 minutes prior to surgery	10/14 (71%)
Tagaya et al²⁵	2010	12	OC (n = 4); LC (n = 8)	Prototype laparoscope and Photo Dynamic Eye	2.5 mg	60 to 120 minutes prior to surgery	12/12 (100%)
Ishizawa et al²⁴	2010	52	LC	Hamamatsu laparoscope	2.5 mg	30 minutes prior to surgery	52/52 (100%)
Hutteman et al²⁶	2011	8	OC^a	Mini-FLARE	5 mg or 10 mg	During surgery	8/8 (100%)
Ishizawa et al¹⁷	2011	7	SILC	Hamamatsu laparoscope	2.5 mg	After intubation	7/7 (100%)
Buchs et al¹⁶	2012	12	SIRC	da Vinci SI System (Intuitive Surgical)	2.5 mg	45 minutes prior to surgery	12/12 (100%)
Kaneko et al²⁸	2012	28	LC	Hamamatsu laparoscope	2.5 mg	15 minutes prior to surgery and during surgery^b	27/28 (96%)
Buchs et al²⁹	2013	23	SIRC	da Vinci SI System (Intuitive Surgical)	2.5 mg	After intubation	NA
Schols et al³¹	2013	30	LC	Karl Storz HD fluorescence endoscopy system	2.5 mg	After intubation and during surgery^b	29/30 (97%)
Schols et al³⁰	2013	15	LC	Karl Storz HD fluorescence endoscopy system	2.5 mg	After intubation	15/15 (100%)
Spinoglio et al³²	2013	45	SIRC	da Vinci SI System (Intuitive Surgical)	2.5 mg	30 minutes prior to surgery	44/45 (98%)
Verbeek et al¹⁵	2014	41	OC (n = 27) LC (n = 14)	Mini-FLARE/Karl Storz	5 mg or 10 mg	24 hours or 30 minutes prior to surgery	41/41 (100%)
Prevot et al³³	2014	23	LC	Karl Storz HD fluorescence endoscopy system	0.05 mg/kg	After intubation	23/23 (100%)
Daskalaki et al¹⁸	2014	184	RC	da Vinci SI System (Intuitive Surgical)	2.5 mg	45 minutes prior to surgery	184/188 (98%)
Larsen et al³⁴	2014	35	LC	Olympus laparoscopic imaging system	0.3-0.4 mg/mL/kg in 5 mL	After intubation	35/35 (100%)
Boni et al³⁸	2015	52	LC	Karl Storz HD fluorescence endoscopy system	0.4 mg/mL/kg	15 minutes prior to surgery and during surgery^b	52/52 (100%)
Dip et al³⁵	2014	43	LC	Karl Storz HD fluorescence endoscopy system	0.05 mg/kg	60 minutes prior to surgery	43/43 (100%)
Kawaguchi et al³⁹	2015	24	LLR	Olympus laparoscopic imaging system	0.025 mg	After intubation	24/24 (100%)
Osayi et al⁴⁰	2015	82	LC	Stryker Infrared Fluorescence (IRF) Imaging System	2.5 mg	60 minutes prior to surgical incision	78/82 (95%)
Dip et al³⁶	2015	45	LC	Karl Storz HD fluorescence endoscopy system	0.05 mg/kg	60 minutes prior to surgery	45/45 (100%)
van Dam et al⁴¹	2015	30	LC	Olympus laparoscopic imaging system	0.05 mg/kg	After intubation	29/30 (97%)
Kono et al¹⁹	2015	108	LC	5 Fluorescence laparoscopic imaging systems^c	2.5 mg	30 minutes prior to surgery and during surgery	99/108 (92%)
Dip et al³⁷	2015	71	LC	Karl Storz HD fluorescence endoscopy system	0.05 mg/kg	60 minutes prior to surgery	100/100 (100%)
Zarrinpar et al²⁰	2016	37	LC + LLR	PinPoint	0.025 mg/kg to 0.25 mg/kg	10 to 180 minutes prior to surgery	NA
Igami et al⁴²	2016	21	LC	Karl Storz HD fluorescence endoscopy system	2.5 mg	After intubation	10/21 (48%)
Total		1057					984/1009 (98%)

Abbreviations: ICG, indocyanine green; OC, open cholecystectomy; LC, laparoscopic cholecystectomy; SILC, single-incision laparoscopic cholecystectomy; SIRC, single-incision robotic cholecystectomy; LLR, laparoscopic liver resection; IR, identification rate; CD, cystic duct.

Open cholecystectomy performed during pancreaticoduodenectomy.

An additional ICG injection during surgery was given to evaluate fluorescence angiograph.

Five fluorescence laparoscopic imaging systems include (1) prototype and (2) improved version of the Hamamatsu Photonics laparoscope, the fluorescence imaging system of Olympus Medical Systems (3), the Karl Storz HD fluorescence laparoscope (4), and the fluorescence imaging system of Novadaq (5).

Patient Characteristics

A total of 28 patients were included. No adverse events related to ICG occurred. Sixteen patients received 5 mg ICG and 12 patients 10 mg ICG. Patient characteristics are summarized in Table 2. Three patients were excluded: one patient due to failed ICG administration, one due to technical issues during saving of images, and one patient with a history of Roux-en-Y gastric bypass who had a percutaneous drain in situ. In 3 patients, an IOC was performed. One patient, with a history of Roux-en-Y gastric bypass, underwent a transgastric endoscopic retrograde cholangiopancreaticography (ERCP) because of choledocholithiasis. Fluorescence cholangiography, performed 30 minutes after injection of 5 mg ICG, did not provide an overview of the bile duct anatomy (BLR 0.3). Another IOC was performed in a patient whose surgery was postponed. Fluorescence imaging 10 hours post dosing of 5 mg ICG was not optimal (BLR 0.4). The third IOC was performed in a patient who received 10 mg ICG 24 hours preoperatively. Fluorescence imaging showed the CBD (BLR 1.1), which was confirmed by IOC. No aberrant bile ducts were detected in the current study, but 2 patients showed aberrant arterial structures in close proximity to the gallbladder. Due to the lack of fluorescence, these structures could be identified as vasculature instead of bile ducts.

Table 2.

Patients’ Characteristics.

Characteristic	5 mg Dose Group	10 mg Dose Group
Number of included patients (n)	16	12
Age, median (range)	61 (20-75)	61 (40-76)
Gender, n (%)
Female	10 (63%)	9 (75%)
Male	6 (37%)	3 (25%)
Body mass index (kg/m²), median (range)	29 (22-37)	27 (22-36)
ASA classification, n (%)
ASA class I	4 (25%)	3 (25%)
ASA class II	12 (75%)	9 (75%)
Preoperative diagnosis
Cholelithiasis	14	11
Choledocholithiasis	5	5
Gallbladder polyp	1	1
Mirizzi syndrome	1	0
Preoperative imaging technique
US	16	12
MRCP	2	4
ERCP	5	6

Abbreviations: US, ultrasound; MRCP, magnetic resonance cholangiopancreaticography; ERCP, endoscopic retrograde cholangiopancreaticography.

Optimization of Dose and Time Interval

Identification of the CD by fluorescence imaging was successful in 24 out of 25 patients (96%). The patient with unsuccessful bile duct identification was a patient who suffered from cirrhosis and Mirizzi syndrome and previously underwent stent placement and ERCP. During the laparoscopic surgery, strong adhesions between stomach, duodenum, and gallbladder were found. No clear overview of bile duct anatomy could be obtained, neither by normal inspection nor using fluorescence imaging. After conversion, the gallbladder appeared completely fused with the proximal duodenum and a fistula between gallbladder and duodenum, and a cyst originating from the CBD was found.

Of the remaining 24 patients, 14 patients received 5 mg ICG and 10 patients 10 mg ICG. Patients were intended to be divided in different groups based on time of fluorescence imaging after ICG injection, but it turned out to be difficult to time the exact moment between ICG administration and surgery due to the unpredictable course of the surgical program. In Figure 2, the BLR is plotted for every individual patient. In the 5 mg dose group, all BLRs between 3 to 7 hours post dosing were >1, with a mean ratio of 1.8 ± 0.5 (Video 1, available online at http://journals.sagepub.com/doi/suppl/10.1177/1553350617702311). Up to 3 hours after dosing, all measurements showed equally or more fluorescent liver tissue compared to the CD (BLR 0.8 ± 0.2), which was significantly lower (P < 0.01; Figure 3). In the 10 mg dose group, all BLRs between 5 and 25 hours were >1, with mean ratios of 1.4 ± 0.3. Up to 5 hours after dosing, the liver showed to be equally or more fluorescent than the CD (BLR 0.9 ± 0.2, respectively), which was again significant (P = 0.02; Figure 4). BLRs 3 to 7 hours post dosing of 5 mg and 5 to 25 hours post dosing of 10 mg were not statistically different (P = 0.12)

Figure 2.

Fluorescence intensity of bile duct compared to adjacent liver tissue for each individual patient. The parabolic lines show our expectation of the mean BLRs over time.

Figure 3.

Fluorescence cholangiography over time after administration of 5 mg ICG. Shown are images of fluorescence detection of the cystic duct (arrow) and adjacent liver tissue (pyramid) over time. Bile duct-to-liver ratios were sufficient (ie, >1) in patients who received 5 mg ICG 3 to 7 hours prior to surgery. BLR was ≤1 when fluorescence imaging was performed less than 3 hours after administration.

Figure 4.

Fluorescence cholangiography over time after administration of 10 mg ICG. Shown are images of fluorescence detection of cystic duct (arrow) and adjacent liver tissue (pyramid) over time. Bile duct-to-liver ratios were sufficient (ie, >1) between 5 to 25 hours post dosing of 10 mg ICG.

Additional Finding

A patient planned to undergo a laparoscopic cholecystectomy and defenestration of a liver cyst received 10 mg ICG. Fluorescence cholangiography was performed at 3.5 hours post dosing. Although bile duct detection was hampered by the intense fluorescence of the liver (BLR 0.7), the liver cyst could be clearly demarcated by the lack of fluorescence inside the cyst (Video 2, available online at http://journals.sagepub.com/doi/suppl/10.1177/1553350617702311).

Evaluation of Fluorescence Cholangiography per Surgeon

Results of the questionnaire among all operating surgeons are displayed in Figure 5.

Figure 5.

Results of questionnaire. Shown are all the scores that were given by surgeons after they performed a laparoscopic cholecystectomy with use of fluorescence cholangiography. The statements that were scored were respectively (I) it could decrease the number of bile duct complications; (II) it led to better detection of bile ducts; (III) the technique was of added value for this procedure; (IV) it needs to be standard-of-care during laparoscopic cholecystectomy; (V) it delays surgery.

Discussion

Fluorescence cholangiography can improve orientation of bile duct anatomy during laparoscopic cholecystectomies and potentially lead to a reduced risk of bile duct injury. This study is the first showing that the interval between administration of ICG and intraoperative fluorescence imaging should be prolonged to 3 to 6 hours post dosing of 5 mg, or 5 to 23 hours post dosing of 10 mg. By doing so, bile ducts are more fluorescent than liver tissue, resulting in improved discrimination of bile duct anatomy. Consequently, also unexpected aberrant bile ducts can be detected.⁴³ This may make laparoscopic cholecystectomy safer. Large studies focusing on patient outcome should demonstrate if fluorescence imaging reduces bile duct injuries.

In our systematic review, none of the identified articles studied fluorescence imaging at the optimal time points. Only 2 studies focused on optimizing the dose of ICG and timing of fluorescence imaging. Zarrinpar et al²⁰ performed a study in patients undergoing laparoscopic hepatic or biliary operations and injected doses ranging between 0.02 and 0.25 mg/kg, 10 to 180 minutes prior to fluorescence imaging. In this study, the authors also concluded that a prolonged interval appeared optimal for fluorescence cholangiography, but they did not study longer time intervals. Our group previously studied fluorescence cholangiography during open and laparoscopic surgery 0.5 and 24 hours after ICG administration.¹⁵ The latter resulted in much better discrimination between bile ducts and liver tissue. However, administrating ICG 24 hours prior to surgery is less feasible in everyday clinical practice; most patients arrive in the morning prior to surgery at the hospital. According to our study results, we can advise a dose of 5 mg ICG, administered 3 to 7 hours prior to surgery, which is much more practical in everyday clinical practice. Although this study is performed with a relatively small group of patients, we believe these results should form the basis for larger studies that focus on the added value of fluorescence cholangiography. Currently, 3 large, multicenter, randomized clinical studies are ongoing to assess the potential added value of fluorescence cholangiography (Table 3). Unfortunately, probably due to logistical reasons, researchers choose to inject ICG directly prior to surgery. Prevention of bile duct injuries is a very difficult endpoint that requires huge amount of patients, but other useful endpoints could be reduced operating room time, cost-effectiveness, less use of radiation for IOC, and so on.

Table 3.

Recruiting randomized controlled trials using fluorescence cholangiography.

Study Coordinator/NCT No.	Participating Country	Number of patients	Start Accrual	Main Outcome	Comparator	Dose ICG	Timing
L. Lehrskov-Schmidt/NCT02344654	Denmark	120	May 2015	Identification BD	X-ray cholangiography	0.05 mg/kg	After intubation
J. van den Bos/NCT02558556	The Netherlands	308	Jan 2016	Time to visualization CVS	White light	2.5 mg	After intubation
R.Rosenthal/NCT02702843	USA	1000	April 2016	Identification BD	White light	0.05 mg/kg	>45 minutes prior to surgery

Abbreviations: BD, bile duct; CVS, critical view of safety.

A commonly mentioned drawback of fluorescence-guided surgery is the lack of standardization of fluorescence imaging systems.⁴⁴ Standardization is important for the determination of objective assessments during oncological fluorescence-guided surgery trials,⁴⁵ but differences in fluorescence imaging systems also hinder comparison between fluorescence cholangiography studies. Different lasers, LEDs, filters, image sensors, and image processing all make a fair comparison difficult. A recent study compared 5 different fluorescence laparoscopic imaging systems for fluorescence cholangiography: a prototype and an improved version of the Hamamatsu Photonics laparoscope, the fluorescence imaging system of Olympus Medical Systems, the Karl Storz HD fluorescence laparoscope, and the fluorescence imaging system of Novadaq.¹⁹ An important conclusion from this study was that the contrast of ICG was significantly different among all the used laparoscopic imaging systems. Outcomes of studies (e.g. BLR) are therefore difficult to compare. The system used in the present study, the Karl Storz HD fluorescence laparoscope, showed not to be optimal for ICG detection, and therefore, ratios obtained in the current study are expected to be even higher when other imaging systems are used.

The intensity of the fluorescence signal depends on several factors, including the distance from the tip of the laparoscope to its target and the amount of covering tissue. For this reason, we chose in the current study to calculate a ratio between the fluorescence signal in the CD and liver, instead of measuring solely fluorescence intensity in the bile duct or liver. Moreover, to make the results more accurate, we analyzed the BLR at 3 different time points during surgery.

Intraoperative assessment of biliary anatomy can be improved using several techniques.⁴⁶ IOC, still the most frequent applied technique for bile duct clarification, is investigated in large cohort studies. A forest plot of the 6 largest population-based studies showed an odds ratio for bile duct injury of 0.60 (95% confidence interval = 0.52-0.70) when using IOC.⁴⁶ No randomized trial studying the effect of IOC exists and is almost impossible to conduct. Still the association between reduced risk of bile duct injury and use of IOC has been shown convincingly.⁴⁷ An important disadvantage of IOC is however that obtained radiographic images can be difficult to interpret and IOC is a technically challenging procedure.⁴⁸ Moreover, costs of IOC are high, caused by prolonged surgery time of 10 to 23 minutes, requirement of an additional medical specialist, and use of equipment.⁴⁹ On the other hand, treating patients with bile duct injuries is a financial burden for the health care system. Some physicians state therefore that routine use of IOC is cost-effective.⁴⁷ As indicated by our surgeons, fluorescence imaging does not prolong operating room time. Furthermore, it does not require another medical specialist or the use of radiation, and it certainly cannot cause bile duct injury.³⁵ We therefore advocate that fluorescence imaging should be an important part of laparoscopic cholecystectomy.

A limiting factor of fluorescence cholangiography compared to IOC is the inability to detect CBD stones. Moreover, the CBD is not always identified, due to the limited penetration depth of approximately 1 cm of light in the NIR spectrum.¹² Structures beneath a layer of (periductal) fat, especially in obese patients, are therefore more difficult to identify using NIR fluorescence imaging.^12,42 Fluorescence cholangiography can however easily be combined with IOC, as shown in the current study. An advantage of fluorescence cholangiography is that it can be performed at all time during surgery without significant delay.

Intravenous ICG administration can also be valuable to assess intraoperative identification of (bile duct) vasculature.^28,31 Because ICG binds to plasma proteins, it remains intravascular.⁵⁰ This in combination with its short half-life of approximately 3 minutes makes it a very useful contrast agent for perfusion angiography. When doubt exists about aberrant vasculature or identification of the cystic artery, an additional injection of ICG can be given. One should however bear in mind that intravenous ICG administration during surgery will result in intense fluorescence of the liver and, thus, should only be performed when bile duct anatomy is already clarified.

In conclusion, this study shows that a prolonged interval (at least 3 hours) between ICG injection and intraoperative fluorescence cholangiography results in much better contrast between bile ducts and liver tissue. Although this study was performed in a small group of patients, we believe that when the optimal dose and timing are used, fluorescence cholangiography can be of added value for both patients and surgeons.

Footnotes

Author Contributions

Study concept and design: Leonora S. F. Boogerd, Henricus J. M. Handgraaf, Wendeline J. van der Made, Cornelis J. H. van de Velde, Alexander L. Vahrmeijer

Acquisition of data: Leonora S. F. Boogerd, Henricus J. M. Handgraaf, Volkert A. L. Huurman, Hwai-Ding Lam, J. Sven D. Mieog, Wendeline J. van der Made

Analysis and interpretation: Leonora S. F. Boogerd, Cornelis J. H. van de Velde, Alexander L. Vahrmeijer

Study supervision: Volkert A. L. Huurman, J. Sven D. Mieog, Wendeline J. van der Made, Cornelis J. H. van de Velde, Alexander L. Vahrmeijer

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported in part by the Dutch Cancer Society Grant UL2010-4732.

References

Nuzzo

Giuliante

Giovannini

. Bile duct injury during laparoscopic cholecystectomy: results of an Italian national survey on 56 591 cholecystectomies. Arch Surg. 2005;140:986-992. doi:10.1001/archsurg.140.10.986.

Törnqvist

Strömberg

Persson

Nilsson

Effect of intended intraoperative cholangiography and early detection of bile duct injury on survival after cholecystectomy: population based cohort study. BMJ. 2012;345:e6457. doi:10.1136/bmj.e6457.

Halbert

Pagkratis

Yang

. Beyond the learning curve: incidence of bile duct injuries following laparoscopic cholecystectomy normalize to open in the modern era. Surg Endosc. 2016;30:2239-2243. doi:10.1007/s00464-015-4485-2.

Halbert

Altieri

Yang

. Long-term outcomes of patients with common bile duct injury following laparoscopic cholecystectomy. Surg Endosc. 2016;30:4294-4299. doi:10.1007/s00464-016-4745-9.

Vettoretto

Saronni

Harbi

Balestra

Taglietti

Giovanetti

Critical view of safety during laparoscopic cholecystectomy. JSLS. 2011;15:322-325. doi:10.4293/108680811X13071180407474.

Polat

Abci

Coskun

Uranues

The importance of intraoperative cholangiography during laparoscopic cholecystectomy. JSLS. 2000;4:103-107.

Halawani

Tamim

Khalifeh

Mailhac

Jamali

FR.

Impact of intraoperative cholangiography on postoperative morbidity and readmission: analysis of the NSQIP database. Surg Endosc. 2016;30:5395-5403. doi:10.1007/s00464-016-4896-8.

El Shallaly

Seow

Sharp

Mughrabi

Nassar

. Intraoperative cholangiography time in laparoscopic cholecystectomy: timing the radiographer. Surg Endosc. 2005;19:1370-1372. doi:10.1007/s00464-004-2242-z.

Way

Stewart

Gantert

. Causes and prevention of laparoscopic bile duct injuries: analysis of 252 cases from a human factors and cognitive psychology perspective. Ann Surg. 2003;237:460-469. doi:10.1097/01.SLA.0000060680.92690.E9.

10.

Mitsuhashi

Kimura

Shimizu

. Usefulness of intraoperative fluorescence imaging to evaluate local anatomy in hepatobiliary surgery. J Hepatobiliary Pancreat Surg. 2008;15:508-514. doi:10.1007/s00534-007-1307-5.

11.

Schaafsma

Mieog

Hutteman

. The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery. J Surg Oncol. 2011;104:323-332. doi:10.1002/jso.21943.

12.

Vahrmeijer

Hutteman

van der Vorst

van de Velde

Frangioni

JV.

Image-guided cancer surgery using near-infrared fluorescence. Nat Rev Clin Oncol. 2013;10:507-518. doi:10.1038/nrclinonc.2013.123.

13.

Boogerd

Handgraaf

Lam

. Laparoscopic detection and resection of occult liver tumors of multiple cancer types using real-time near-infrared fluorescence guidance. Surg Endosc. 2016;31:952-961. doi:10.1007/s00464-016-5007-6.

14.

Benya

Quintana

Brundage

Adverse reactions to indocyanine green: a case report and a review of the literature. Cathet Cardiovasc Diagn. 1989;17:231-233.

15.

Verbeek

Schaafsma

Tummers

. Optimization of near-infrared fluorescence cholangiography for open and laparoscopic surgery. Surg Endosc. 2014;28:1076-1082. doi:10.1007/s00464-013-3305-9.

16.

Buchs

Hagen

Pugin

. Intra-operative fluorescent cholangiography using indocyanin green during robotic single site cholecystectomy. Int J Med Robot. 2012;8:436-440. doi:10.1002/rcs.1437.

17.

Ishizawa

Kaneko

Inoue

. Application of fluorescent cholangiography to single-incision laparoscopic cholecystectomy. Surg Endosc. 2011;25:2631-2636. doi:10.1007/s00464-011-1616-2.

18.

Daskalaki

Fernandes

Wang

. Indocyanine green (ICG) fluorescent cholangiography during robotic cholecystectomy: results of 184 consecutive cases in a single institution. Surg Innov. 2014;21:615-621. doi:10.1177/1553350614524839.

19.

Kono

Ishizawa

Tani

. Techniques of fluorescence cholangiography during laparoscopic cholecystectomy for better delineation of the bile duct anatomy. Medicine (Baltimore). 2015;94:e1005. doi:10.1097/md.0000000000001005.

20.

Zarrinpar

Dutson

Mobley

. Intraoperative laparoscopic near-infrared fluorescence cholangiography to facilitate anatomical identification: when to give indocyanine green and how much. Surg Innov. 2016;23:360-365. doi:10.1177/1553350616637671.

21.

Cherrick

Stein

Leevy

Davidson

CS.

Indocyanine green: observations on its physical properties, plasma decay, and hepatic extraction. J Clin Invest. 1960;39:592-600.

22.

Ishizawa

Tamura

Masuda

. Intraoperative fluorescent cholangiography using indocyanine green: a biliary road map for safe surgery. J Am Coll Surg. 2009;208:e1-e4. doi:10.1016/j.jamcollsurg.2008.09.024.

23.

Aoki

Murakami

Yasuda

. Intraoperative fluorescent imaging using indocyanine green for liver mapping and cholangiography. J Hepatobiliary Pancreat Sci. 2010;17:590-594. doi:10.1007/s00534-009-0197-0.

24.

Ishizawa

Bandai

Ijichi

Kaneko

Hasegawa

Kokudo

Fluorescent cholangiography illuminating the biliary tree during laparoscopic cholecystectomy. Br J Surg. 2010;97:1369-1377. doi:10.1002/bjs.7125.

25.

Tagaya

Shimoda

Kato

. Intraoperative exploration of biliary anatomy using fluorescence imaging of indocyanine green in experimental and clinical cholecystectomies. J Hepatobiliary Pancreat Sci. 2010;17:595-600. doi:10.1007/s00534-009-0195-2.

26.

Hutteman

van der Vorst

Mieog

. Near-infrared fluorescence imaging in patients undergoing pancreaticoduodenectomy. Eur Surg Res. 2011;47:90-97. doi:10.1159/000329411.

27.

Ishizawa

Fukushima

Shibahara

. Real-time identification of liver cancers by using indocyanine green fluorescent imaging. Cancer. 2009;115:2491-2504. doi:10.1002/cncr.24291.

28.

Kaneko

Ishizawa

Masuda

. Indocyanine green reinjection technique for use in fluorescent angiography concomitant with cholangiography during laparoscopic cholecystectomy. Surg Laparosc Endosc Percutan Tech. 2012;22:341-344. doi:10.1097/SLE.0b013e3182570240.

29.

Buchs

Pugin

Azagury

. Real-time near-infrared fluorescent cholangiography could shorten operative time during robotic single-site cholecystectomy. Surg Endosc. 2013;27:3897-3901. doi:10.1007/s00464-013-3005-5.

30.

Schols

Bouvy

Masclee

van Dam

Dejong

Stassen

LP.

Fluorescence cholangiography during laparoscopic cholecystectomy: a feasibility study on early biliary tract delineation. Surg Endosc. 2013;27:1530-1536. doi:10.1007/s00464-012-2635-3.

31.

Schols

Bouvy

van Dam

Masclee

Dejong

Stassen

LP.

Combined vascular and biliary fluorescence imaging in laparoscopic cholecystectomy. Surg Endosc. 2013;27:4511-4517. doi:10.1007/s00464-013-3100-7.

32.

Spinoglio

Priora

Bianchi

. Real-time near-infrared (NIR) fluorescent cholangiography in single-site robotic cholecystectomy (SSRC): a single-institutional prospective study. Surg Endosc. 2013;27:2156-2162. doi:10.1007/s00464-012-2733-2.

33.

Prevot

Rebibo

Cosse

Browet

Sabbagh

Regimbeau

JM.

Effectiveness of intraoperative cholangiography using indocyanine green (versus contrast fluid) for the correct assessment of extrahepatic bile ducts during day-case laparoscopic cholecystectomy. J Gastrointest Surg. 2014;18:1462-1468. doi:10.1007/s11605-014-2560-y.

34.

Larsen

Schulze

Bisgaard

Non-radiographic intraoperative fluorescent cholangiography is feasible. Dan Med J. 2014;61:A4891.

35.

Dip

Asbun

Rosales-Velderrain

. Cost analysis and effectiveness comparing the routine use of intraoperative fluorescent cholangiography with fluoroscopic cholangiogram in patients undergoing laparoscopic cholecystectomy. Surg Endosc. 2014;28:1838-1843. doi:10.1007/s00464-013-3394-5.

36.

Dip

Roy

Lo Menzo

Simpfendorfer

Szomstein

Rosenthal

RJ.

Routine use of fluorescent incisionless cholangiography as a new imaging modality during laparoscopic cholecystectomy. Surg Endosc. 2015;29:1621-1626. doi:10.1007/s00464-014-3853-7.

37.

Dip

Nguyen

Montorfano

. Accuracy of near infrared-guided surgery in morbidly obese subjects undergoing laparoscopic cholecystectomy. Obes Surg. 2016;26:525-530. doi:10.1007/s11695-015-1781-9.

38.

Boni

David

Mangano

. Clinical applications of indocyanine green (ICG) enhanced fluorescence in laparoscopic surgery. Surg Endosc. 2015;29:2046-2055. doi:10.1007/s00464-014-3895-x.

39.

Kawaguchi

Velayutham

Fuks

Christidis

Kokudo

Gayet

Usefulness of indocyanine green-fluorescence imaging for visualization of the bile duct during laparoscopic liver resection. J Am Coll Surg. 2015;221:e113-e117. doi:10.1016/j.jamcollsurg.2015.09.001.

40.

Osayi

Wendling

Drosdeck

. Near-infrared fluorescent cholangiography facilitates identification of biliary anatomy during laparoscopic cholecystectomy. Surg Endosc. 2015;29:368-375. doi:10.1007/s00464-014-3677-5.

41.

van Dam

Ankersmit

van de Ven

van Rijswijk

Tuynman

Meijerink

WJ.

Comparing near-infrared imaging with indocyanine green to conventional imaging during laparoscopic cholecystectomy: a prospective crossover study. J Laparoendosc Adv Surg Tech A. 2015;25:486-492. doi:10.1089/lap.2014.0248.

42.

Igami

Nojiri

Shinohara

. Clinical value and pitfalls of fluorescent cholangiography during single-incision laparoscopic cholecystectomy. Surg Today. 2016;46:1443-1450. doi:10.1007/s00595-016-1330-8.

43.

Calatayud

Milone

Elli

Giulianotti

PC.

ICG-fluorescence identification of a small aberrant biliary canaliculus during robotic cholecystectomy. Liver Int. 2012;32:602. doi:10.1111/j.1478-3231.2012.02757.x.

44.

Snoeks

van Driel

Keereweer

. Towards a successful clinical implementation of fluorescence-guided surgery. Mol Imaging Biol. 2014;16:147-151. doi:10.1007/s11307-013-0707-y.

45.

Rosenthal

Warram

de Boer

. Successful translation of fluorescence navigation during oncologic surgery: a consensus report. J Nucl Med. 2016;57:144-150. doi:10.2967/jnumed.115.158915.

46.

Buddingh

Nieuwenhuijs

van Buuren

Hulscher

de Jong

van Dam

GM.

Intraoperative assessment of biliary anatomy for prevention of bile duct injury: a review of current and future patient safety interventions. Surg Endosc. 2011;25:2449-2461. doi:10.1007/s00464-011-1639-8.

47.

Flum

Flowers

Veenstra

DL.

A cost-effectiveness analysis of intraoperative cholangiography in the prevention of bile duct injury during laparoscopic cholecystectomy. J Am Coll Surg. 2003;196:385-393. doi:10.1016/S1072-7515(02)01806-9.

48.

Buddingh

Weersma

Savenije

van Dam

Nieuwenhuijs

VB.

Lower rate of major bile duct injury and increased intraoperative management of common bile duct stones after implementation of routine intraoperative cholangiography. J Am Coll Surg. 2011;213:267-274. doi:10.1016/j.jamcollsurg.2011.03.004.

49.

Ford

Soop

Loveday

Rodgers

Systematic review of intraoperative cholangiography in cholecystectomy. Br J Surg. 2012;99:160-167. doi:10.1002/bjs.7809.

50.

Schaafsma

Mieog

Hutteman

. The clinical use of indocyanine green as a near-infrared fluorescent contrast agent for image-guided oncologic surgery. J Surg Oncol. 2011;104:323-332. doi:10.1002/jso.21943.