The Role of Leukotriene B4 in Chronic Venous Insufficiency

Abstract

In this study the authors evaluate polymorphonuclear (PMN) cell activation by measuring the level of leukotriene B4 (LTB4) in patients presenting chronic venous insufficiency. The blood samples were drawn from affected lower limbs and from healthy upper limbs for comparison. One portion of the PMN, obtained from blood samples, was stimulated with calcium ionophore A23187. The results showed an increase of mean LTB4 levels in both districts, particularly in the lower limb, under basal conditions. Following calcium ionophore stimulation, the LTB4 concentrations in the lower limb did not evidence signif icant changes compared with the basal levels, while they increased in the upper district. These results point to the existence of various degrees of leukocyte activation: the highest at the pathologic site, with lower levels at the other sites. Also noted was a variability of the LTB4 levels between the different individuals in the areas examined, owing probably to subjective, genetic expression for LTB4 receptors and other adhesion molecules.

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References

Perego MA , Danese C. , Zavattaro E. : Reologia leucocitaria nell'insufficienza venosa. Atti del V Congresso Nazionale della Societa' Italiana di Emoreologia Clinica, Palermo, Septtember, 1992, pp 257-261.

Danese C. , Zavattaro E. , Calisi L. , et al: Parametri leucoreologici nelle varicoflebiti in fase acuta. Atti del V Congresso Nazionale della Societa' Italiana di Emorelogia Clinica , Palermo, Septtember, 1992, pp 275-277.

Danese C. , Fusco P. , Renzulli C. , et al: Variazioni emoreologiche posturali e distrettuali in soggetti sani. Atti del V Cngresso Nazionale della Societa' Italiana di Emoreologia Clinica, Palermo, Septtember, 1992, pp 409-411.

Browse NL , Burnand KG : The cause of venous ulceration. Lancet ii:243-245, 1982.

Moyses C. , Cederholm-Williams SA , Michel CC : Hemoconcentration and accumulation of white cells in the feet during venous stasis. Int J Microcirc Clin Exp 5:311-320, 1987.

Thomas Prs , Nash GB , Dormandy JA : White cell accumulation in dependent legs of patients with venous hypertension: A possible mechanism for trophic changes in the skin. Br Med J 296:1693-1695, 1988.

Danese C. , Cifone MG , Zavattaro E. , et al: Leukotriene B4 (LTB4) in primary venous insufficiency. Int Angiology 1995; 14 (suppl 1):323, 1995 (abstract).

Stewart GJ : Neutrophils and deep venous thrombosis. Haemostasis 23 (suppl 1):127-140, 1993.

Goldman DW : Regulation of receptor system for leukotriene B4 on human neutrophils . Ann NY Acad Science 524:187-195, 1988.

10.

Michielis C. , Arnould T. , Remacle J. : Interactions between endothelial and smooth muscle cells after their activation by hypoxia. A possible aetiology for venous disease. Int Angiology 14 (suppl 1):108-109, 1995 (abstract).

11.

Milhoan KA , Lane TA , Bloor CM : Hipoxia induces endothelial cells to increase their adherence for neutrophils: Role of PAF. Am J Physiol 3 (Pt 2):H956-H962, 1992.

12.

Michielis C. , Arnould T. , Janssens D. , et al: Hypoxia increases the adherence of neutrophils to endothelium: Effect of ginkor fort. Int Angiology 14 (suppl 1):221, 1995 (abstract).

13.

Michielis C. , Arnould T. , Remacle J. : Hypoxia-induced activation of endothelial cells as a possible cause of venous disease: Hypothesis. Angiology 44:639-646, 1993.

14.

Dormandy JA : Influence of blood cells and mediators on endothelium . Int Angiology 14 (suppl 1):108, 1995 (abstract).