Sage Journals: Discover world-class research

Abstract

Objective: To investigate whether intraluminal administration of oxygenated perfluorocarbons (PFCs) protects the enterocyte from acute ischemia-reperfusion (I/R) injury. Materials and Methods: Twenty rabbits were divided in 4 groups: sham-operated controls (group A), acute I/R (group B), acute I/R plus infusion of oxygenated PFCs 30 minutes before ischemia (group C), and acute I/R plus infusion of oxygenated PFCs 30 minutes before reperfusion (group D). Serum creatine phosphokinase (CPK) and mucosal disaccharidase activity were examined. Intestinal biopsies were obtained for electron microscopy study. Results: Group B CPK mean values are 3495.2 ± 157.35 and 4855 ± 350.21 U/L. Group C: 2674.6 ± 265.87 and 3231 ± 232.30. Group D: 2382.2 ± 102.90 and 3217.6 ± 185.61 at 120 and 180 minutes (P < .05). At 180 minutes, maltase and sucrose values were 33.63, 51.88, 8.45, and 19.91, and 17.99, 22.87, 6.62, and 14.24 µmol/min per g for groups A, B, C, and D, respectively (P < .05). Histopathology showed the least cellular deterioration in PFC groups. Conclusion: Oxygenated PFCs protect the enterocyte during bowel I/R.

Keywords

intestinal I/R injury perfluorocarbons creatine phosphokinase disaccharidase activity

Get full access to this article

View all access options for this article.

References

Zimmerman JE , Knaus WA , Wagner DP Severity stratification and outcome prediction for multisystem organ failure and dysfunction. World J Surg . 1996;20(4):401-405.

de Perrot M. , Liu M. , Waddell TK , Keshavjee S. Ischemia reperfusion-induced lung injury. Am J Resp Crit Care Med. 2003 ;167(4):490-511.

Grotz MRW , Deitch EA , Ding J. , Xu D. , Huang Q. , Regel G. Intestinal cytokine response after gut ischemia: role of gut barrier failure . Ann Surg. 1999 ;229(4):478-486.

Mohan C. , Gennaro M. , Marini C. , Ascer E. Reduction of ischemic skeletal muscle necrosis by perfusion with oxygenated perfluorocarbon. Am J Surg. 1992;164(3):194-198.

Moore EE , Moore FA , Franciose RJ , et al. The postischemic gut serves as a priming bed for circulating neutrophils that provoke multiple organ failure. J. Trauma . 1994;37(6):881.

O’Donnell KA , Caty MG , Zheng S. , Rossman JE , Azizkhan RG Oxygenated intraluminal perfluorocarbon protects intestinal muscosa from ischemia/reperfusion injury. J Pediatr Surg. 1997;32(2):361-365.

Papadimitriou DK , Pitoulias GA , Kotakidou RE , Alvanou Achparaki AE , Kaidoglou Anagnostopoulou EN Prolongation of the intestinal viability using oxygenated perfluorocarbon in an experimental model of acute intestinal ischemia. Eur J Vasc Endovasc Surg. 2004;28(6):636-641.

Ntinas A. , Iliadis S. , Alvanou-Achaparaki A. , et al. The protective effect of oxygenated perfluorocarbons (PFCs) on intestinal ischemia-reperfusion injury (I/R) in rabbits. J Vasc Endovasc Surg. 2010;44(2):81-88.

Sloviter HA , Muckerji B. Prolonged retention in the circulation of emulsified lipid-coated perflurochemicals . Prog Clin Biol Res. 1983;122:181-187.

10.

Dahlqvist A. Method for assay of intestinal dissacharidases . Anal Biochem . 1964;7:18-25.

11.

Riess JG Perfluorocarbon-based Oxygen Delivery. Artif Cells Blood Substit Immobil Biotechnol. 2006;34(6):567-580.

12.

Gao C. , Xu L. , Chai W. , Sun X. , Zhang H. , Zhang G. Amelioration of intestinal ischemia-reperfusion injury with intraluminal hyperoxygenated solution: studies on structural and functional changes of enterocyte mitochondria . J Surg Res. 2005 ;129(2): 298-305.

13.

Oldham KT , Guise KS , Gore D. , Gourley WK , Lobe TE Treatment of intestinal ischemia with oxygenated intraluminal perfluorocarbons . J Surg. 1987;153(3):291-420.

14.

Vejchapipat P. , Proctor E. , Ramsay A. , Petros A. , Gadian GD , Spitz L. , Pierro A. Intestinal energy metabolism after ischemiareperfusion: effects of moderate hypothermia and perfluorocarbons. J Pediatr Surg. 2002;37(5):786-790.

15.

Çaglayan F. , Çaglayan O. , Gunel E. , Elcuman Y. , Çakmak M. Intestinal ischemia-reperfusion and plasma enzyme levels. J Pediatr Surg . 2002;18(4):255-257.

16.

Fujino Y. , Suzuki Y. , Kakinoki K. , Tanioka Y. , Ku Y. , Kuroda Y. Protection against experimental ischaemia-reperfusion injury with oxyganated perfluorochemical. Brit J Surg. 2003;90(8):1015-1020.

17.

Hening SJ Ontogeny of enzymes in the small intestine. Am Rev Physiol . 1985;47:231-245.

Oxygenated Perfluorocarbons Protect the Intestine From the Ischemia/Reperfusion Injury in Rabbits

Abstract

Keywords

Get full access to this article

References