Green synthesized silver nanoparticles for the treatment of diabetes and the related complications of hyperlipidemia and oxidative stress in diabetic rats
Restricted accessResearch articleFirst published online December, 2023
Green synthesized silver nanoparticles for the treatment of diabetes and the related complications of hyperlipidemia and oxidative stress in diabetic rats
This study was conducted to compare the impact of cinnamon silver nanoparticles (C-Ag-NPs) and cinnamon aqueous extract (CAE) on the total body weight (TBW), body weight gain (BWG), blood count (BC), fasting blood glucose (FBG), triglycerides (TGs), total cholesterol (TC), low-density (LDL-C) and high-density (HDL-C) lipoprotein cholesterol, liver function enzymes, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) of normal and streptozotocin (STZ) diabetic rats. The CAE was administered to rats at different doses (50.0 and 100.0 mg/kg bw), whereas the C-Ag-NPs were ingested at doses of 25.0 and 50.0 mg/kg bw for 30 days. At the end of the experiment, the administration of high or low dosages of CAE or C-Ag-NPs to diabetic rats significantly reduced the FBG, TC, TG, and LDL-C and significantly increased the HDL-C compared with the diabetic control rats. The highest dose (50.0 mg/kg bw) of the C-Ag-NPs was the most efficient at significantly reducing (P < 0.05) the levels of all the analyzed parameters compared with the CAE. However, the treated and normal rats did not show any hypoglycemic activity after ingesting the CAE or C-Ag-NPs. Such effects were associated with considerable increases in their BWG. The diabetic rats that ingested the CAE or C-Ag-NPs showed a gradual decrease in their FBG, TC, LDL, and TG levels, but they were still higher than those in the normal rats. Furthermore, the C-Ag-NPs and CAE considerably enhanced the hepatic (GPT, GOT, ALP, and GGT) and antioxidant biomarker enzyme activities (SOD, CAT, and GPx) in diabetic rats. Relative to the untreated diabetic control, the C-Ag-NPs were more effective than the CAE in the diabetic rats. The C-Ag-NPs exhibited a protective role against hyperglycemia and hyperlipidemia in the diabetic rats and modulated their liver function enzyme biomarkers and antioxidant enzyme activities more than the CAE.
WildSRoglicGGreenASicreeRKingH. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030.Diabetes Care2004;27:1047–53
3.
AroraMKSinghUK. Oxidative stress: meeting multiple targets in pathogenesis of diabetic nephropathy.Curr Drug Targets2014;15:531–8
4.
NguyenDVShawLCGrantMB. Inflammation in the pathogenesis of microvascular complications in diabetes.Front Endocrinol2012;3:170–6
5.
Ali RazaJMovahedA. Current concepts of cardiovascular diseases in diabetes mellitus.Int J Cardiol2003;89:123–34
6.
World Health Organization. Document–WHO/NCD/NCS/99.2: definition, diagnosis and classification of diabetes mellitus and its complications.http://whqlibdoc.who.int/hq/1999/WHO_NCD_NCS_99.2.pdf (1999, accessed 3 March 2023)
7.
NyunaïNNjikamNAbdennebEMbaforJLamnaouerD. Hypoglycaemic and antihyperglycaemic activity of Ageratum Conyzoides L. in rats.Afr J Tradi Complement Altern Med2009;6:123–30
8.
BroadhurstCLPolanskyMMAndersonRA. Insulin–like biological activity of culinary and medicinal plant aqueous extracts in vitro.J Agric Food Chem2000;48:849–52
9.
MarlesRJFarnsworthNR. Antidiabetic plants and their active constituents.Phytomedicine1995;2:137–89
10.
AlsoodeeriFNAlqabbaniHMAldossariNM. Effects of cinnamon (Cinnamomum cassia) consumption on serum lipid profiles in albino rats.J Lipids2020;2020:8469830
11.
El–BazYGMoustafaAAliMAEl–DesokyGEWabaidurSMFaisalMM. An analysis of the toxicity, antioxidant, and anti–cancer activity of cinnamon silver nanoparticles in comparison with extracts and fractions of cinnamomum cassia at normal and cancer cell levels.Nanomaterials2023;13:945–63
12.
HuangYCChenBH. A comparative study on improving streptozotocin–induced type 2 diabetes in rats by hydrosol, extract and nanoemulsion prepared from cinnamon leaves.Antioxidants2022;12:29–50
13.
El–DesokyGEAboul–SoudMAAl-NumairKS. Antidiabetic and hypolipidemic effects of Ceylon cinnamon (Cinnamomum verum) in alloxan–diabetic rats.J Med Plants Res2012;6:1685–91
14.
HusseinZM. The effect of nano–cinnamomum capsule on blood glucose, and lipid profile in type 2 diabetic male rats.J Pharm Negat Results2022;13:709–14
15.
UlbrichtCSeamonEWindsorRCArmbruesterNBryanJKCostaDGieseNGruenwaldJIovinRIsaacRSerranoJMTanguay-ColucciSWeissnerWYoonHZhangJ. An evidence–based systematic review of cinnamon (Cinnamomum spp.) by the Natural Standard Research Collaboration.J Diet Suppl2011;8:378–454
16.
ShengXZhangYGongZHuangCZangY. Improved insulin resistance and lipid metabolism by cinnamon extract through activation of peroxisome proliferator–activated receptors.PPAR Res2008;2008:581348
17.
Jarvill-TaylorKJAndersonRAGravesDJ. A hydroxychalcone derived from cinnamon functions as a mimetic for insulin in 3T3– L1 adipocytes.J Am Coll Nutr2001;20:327–36
18.
SubhasreeSAnithaPKannanKRamachandranASheriJJJayavelR. Anticorrosion behavior of ZnO nanoparticles coated on mild steel in NaCl solution.J Nanosci Nanotechnol2020;20:4061–8
19.
SolaimanMAAliMAAbdel-MoeinNMMahmoudEA. Synthesis of Ag–NPs developed by green–chemically method and evaluation of antioxidant activities and anti–inflammatory of synthesized nanoparticles against LPS–induced NO in RAW 264.7 macrophages.Biocatal Agric Biotechnol2020;29:101832
20.
El-DesokyGEWabaidurSMAlOthmanZAHabilaMA. Evaluation of nano–curcumin effects against tartrazine–induced abnormalities in liver and kidney histology and other biochemical parameters.Food Sci Nutr2022;10:1344–56
21.
Abo-El-YazidZHAhmedOKEl-TholothMAliMA. Green synthesized silver nanoparticles using Cyperus rotundus L. extract as a potential antiviral agent against infectious laryngotracheitis and infectious bronchitis viruses in chickens.Chem Biol Technol Agric2022;9:55
22.
AhmedMMarrezDAAbdelmoeenNMMahmoudEAAbdel–ShakurAli MDecsiKTóthZ. Proximate analysis of moringa oleifera leaves and the antimicrobial activities of successive leaf ethanolic and aqueous extracts compared with green chemically synthesized Ag–NPs and crude aqueous extract against some pathogens.Int J Mol Sci2023;24:3529–48
23.
GauthamiMSrinivasanNMGoudNBoopalanKThirumuruganK. Synthesis of silver nanoparticles using cinnamomum zeylanicum bark extract and its antioxidant activity.Nanosci Nanotechnol–Asia2015;5:27
24.
SathishkumarMSnehaKKwakISMaoJTripathySYunYS. Phyto–crystallization of palladium through reduction process using Cinnamom zeylanicum bark extract.J Hazard Mater2009;171:400–4
25.
AwwadAMSalemNMAbdeenAO. Biosynthesis of silver nanoparticles using Olea europaea leaves extract and its antibacterial activity.Nanosci Nanotechnol2012;2:164–70
26.
SrinivasNOuldridgeTESulcPSchaefferJMYurkeBLouisAADoyeJPWinfreeE. On the biophysics and kinetics of toehold–mediated DNA strand displacement.Nucleic Acids Res2013;41:10641–58
27.
YadavSVatsVDhunnoYGroverJK. Hyperglycemic and antihyperglycemic activity of Muraya koenigii leaves in diabetic rats.J Ethnopharmacol2002;82:111–6
28.
KouameKPeterAIAkangENMoodleyRNaiduECAzuOO. Histological and biochemical effects of Cinnamomum cassia nanoparticles in kidneys of diabetic Sprague–Dawley rats.Bosn J Basic Med Sci2019;19:138–45
29.
TietzF. Thermal expansion of SOFC materials.Ionics1999;5:129–39
30.
TrinderP. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor.Ann Clin Biochem1969;6:24–7
31.
FriedwaldWTLeveRIFredrichsonDS. Estimation of concentration of low density lipoproteins separated by three different methods.Clin Chem1972;18:499–502
32.
ReitmanSFrankelS. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases.Am J Clin Pathol1957;28:56–63
33.
SchumannGBonoraRCeriottiFFérardGFerreroCAFranckPFGellaF-JHoelzelWJørgensenPJKannoTKessnerAKlaukeRKristiansenNLessingerJ-MLinsingerTPJMisakiHPanteghiniMPauwelsJSchieleFSchimmelHG. IFCC primary reference procedures for the measurement of catalytic activity concentrations of enzymes at 37 degrees C. International Federation of Clinical Chemistry and Laboratory Medicine. Part 4. Reference procedure for the measurement of catalytic concentration of alanine aminotransferase.Clin Chem Lab Med2002;40:718–24
34.
FukeHYagiHTakegoshiCKondoT. A sensitive automated colorimetric method for the determination of serum gamma–glutamyl transpeptidase.Clin Chim Acta1976;69:43–51
PagliaDEValentineWN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.J Lab Clin Med1967;70:158–69
37.
SameenAFathimaSJRamlalSKumarSKhanumF. Nanopackaging of silver using spice extract and their characterization.Sci Technol Arts Res J2014;3:52–6
38.
BanerjeePSatapathyMMukhopahayayADasP. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis.Biores Bioproces2014;1:1–10
39.
HuangQLiDSunYLuYSuXYangHWangYWangWShaoNHongJChenC. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf.Nanotechnology2007;18:105104
40.
PriyaBGuptaVKPathaniaDSinghaAS. Synthesis, characterization and antibacterial activity of biodegradable starch/PVA composite films reinforced with cellulosic fibre.Carbohydr Polym2014;109:171–9
41.
ForoughMFarhadiK. Biological and green synthesis of silver nanoparticles.Turkish J Eng Environ Sci2010;34:281–7
42.
LongeAOMomohJAdepojuPA. Effects of cinnamon aqueous extract on blood glucose level, liver biomarker enzymes, hematological and lipid profile parameters in alloxan–induced diabetic male albino rats.Eur Sci J2015;1:418–26
43.
AzeemMNAAhmedOMShabanMElsayedKNM. In vitro antioxidant, anticancer, anti–inflammatory, anti–diabetic and anti–Alzheimer potentials of innovative macroalgae bio–capped silver nanoparticles.Environ Sci Pollut Res Int2022;29:59930–47
44.
GovernaPBainiGBorgonettiVCettolinGGiachettiDMagnanoARMiraldiEBiagiM. Phytotherapy in the management of diabetes: a review.Molecules2018;23:105–26
45.
RekhaNBalajiRDeecaramanM. Antihyperglycemic and antihyperlipidemic effects of extracts of the pulp of Syzygium cumini and bark of Cinnamon zeylanicum in streptozotocin–induced diabetic rats.J Appl Biosci2010;28:1718–30
46.
AlagammalMNishanthiniAMohanVR. Antihyperglycemic and antihyperlipidaemic effect of Polygala rosmarinifolia Wright & Arn on alloxan induced diabetic rats.J Appl Pharm Sci2012;2:143–8
47.
PingHZhangGRenG. Antidiabetic effects of cinnamon oil in diabetic KK–Ay mice.Food Chem Toxicol2010;48:2344–9
48.
BlevinsSMLeyvaMJBrownJWrightJScofieldRHAstonCE. Effect of cinnamon on glucose and lipid levels in non–insulin–dependent type 2 diabetes.Diabetes Care2007;30:2236–7
49.
QinBNagasakiMRenMBajottoGOshidaYSatoY. Cinnamon extract (traditional herb) potentiates in vivo insulin–regulated glucose utilization via enhancing insulin signaling in rats.Diabetes Res Clin Pract2003;62:139–48
50.
GiboneyPT. Mildly elevated liver transaminase levels in the asymptomatic patient.Am Fam Physician2005;71:1105–10
51.
VijayalingamSParthibanAShanmugasundaramKRMohanV. Abnormal antioxidant status in impaired glucose tolerance and non-insulin-dependent diabetes mellitus.Diabet Med1996;13:715–9
