This study was designed to examine the alloantigen system L effects on Rous sarcomas in three B complex genotypes. The parental stock was 50% Modified Wisconsin Line 3 × White Leghorn Line NIU 4 and 50% inbred Line 6.15-5. Pedigree matings of two B2B5 L1L2 sires to five B2B5 L1L2 dams per sire produced experimental chicks segregating for B and L genotypes. Chicks were inoculated with 20 pock-forming units (pfu) of Rous sarcoma virus (RSV) at 6 weeks of age. Tumors were scored six times over 10 weeks postinoculation after which the tumor scores were used to assign a tumor profile index (TPI) to each chicken. Tumor growth over time and TPl were evaluated by repeated-measures analysis of variance and analysis of variance, respectively. Six trials were conducted with a total of 151 chickens. The major histocompatibility (B) complex affected the responses as the B2B2 and B2B5 genotypes had significantly lower tumor growth over time and TPl than the B5B5 genotype. Separate analyses revealed no significant L system effect in B2B2 or B2B5 backgrounds. However, L genotype significantly affected (P < 0.05) both tumor growth over time and TPl in B5B5 chickens. B5B5 L1L2 birds had TPl significantly lower than B5B5 L1L1 chickens but not B5B5 L2L2. Mortality was lower in the B5B5 L1L2 birds than in B5B5 L2L2 chickens. The L system, or one closely linked, affects the growth and ultimate outcome of Rous sarcomas. The response may depend upon the genetic background as well as MHC type.
BrilesWE, McGibbonWH, IrwinMR. On multiple alleles effecting cellular antigens in the chicken. Genetics35: 633–652, 1950.
3.
SchiermanLW, NordskogAW. Relationship of blood type to histocompatibility in chickens. Science134: 1008–1009, 1961.
4.
DietertRR, TaylorRLJr., DietertMF. Biological functions of the chicken major histocompatibility complex. Crit Rev Poult Biol3: 111–129, 1991.
5.
KaufmanJ, SalomonsenJ.The “minimal essential Mhc” revisited: both peptide-binding and cell surface expression of MHC molecules are polymorphisms selected by pathogens in chickens. Hereditas127: 67–73, 1997.
6.
GilmourDG. Segregation of genes determining red cell antigens at high levels of inbreeding in chickens. Genetics44: 14–33, 1959.
7.
BrilesWE. Additional blood group systems in the chicken. Ann NY Acad Sci97: 173–183, 1962.
8.
CrittendenLB, ProvencherL, SantangeloL, LevinI, AbplanalpH, BrilesRW, BrilesWE, DodgsonJB. Characterization of a red jungle fowl by white Leghorn backcross reference population for molecular mapping of the chicken genome. Poult Sci72: 334–348, 1993.
9.
ScottTR, OduhoGW, GlickB, HaganF, BrilesWE, YamamotoY.Erythrocyte alloantigen diversity and some immunological effects of the B system in related New Hampshire strains. Poult Sci67: 1210–1217, 1988.
10.
DeSilvaPLG. Heterozygocity at red cell antigen locus L and fertility in chickens. Genetics51: 41–48, 1965.
11.
TaylorRLJr., BrilesWE. Non-MHC alloantigen effects on resistance and susceptibility to Eimeria tenella. Poultry Sci79:(Suppl. 1):39, 2000.
12.
CollinsWM, BrilesWE, ZsigrayRM, DunlopWR, CorbettAC, ClarkKK, MarksJL, McGrailTP. The B locus (MHC) in the chicken: association with the fate of RSV-induced tumors. Immunogenetics5: 333–343, 1977.
13.
SchiermanLW, WatanabeDH, McBrideRA. Genetic control of Rous sarcoma regression in chickens: linkage with the major histocompatibility complex. Immunogenetics5: 325–332, 1977.
14.
BrownDW, CollinsWM, ZsigrayRM, BrilesWE. A non-MHC genetic influence on response to Rous sarcoma virus-induced tumors in chickens. Avian Dis28: 884–899, 1984.
15.
PlachyJ, HalaK, BendaV.Regression of tumors induced by virus in Rous sarcoma different inbred lines of chickens. Folia Biol (Praha)25: 335–336, 1979.
16.
PlachyJ.Hierarchy of the B (MHC) haplotypes controlling resistance to Rous sarcomas in a model of inbred lines of chickens. Folia Biol (Praha)30: 412–425, 1984.
17.
PlachyJ, BendaV, KarakozI, SpatenkovaZ.Different importance of primary lymphoid organs for Rous sarcoma regression in inbred lines of chickens. Folia Biol (Praha)30: 177–188, 1984.
18.
CollinsWM, HeinzelmannEW, CorbettAC, ZsigrayRM, DunlopWR. Rous sarcoma regression in seven highly inbred lines of white leghorns. Poult Sci59: 1172–1177, 1980.
19.
GilmourDG, CollinsWM, FredricksenTL, AuclairB, ClarkKK, UrbanWEJr.Influence of non-MHC T lymphocyte alloantigens on regression of Rous sarcomas in the chicken. Immunogenetics17: 43–54, 1983.
20.
GilmourDG, CollinsWM, FredricksenTL, UrbanWE. Genetic interaction between non-MHC T- and B-cell alloantigens in response to Rous sarcomas in chickens. Immunogenetics23: 1–6, 1986.
21.
CollinsWM, BrilesWE, CorbettAC, ClarkKK, ZsigrayRM, DunlopWR. B locus (MHC) effect upon regression of RSV-induced tumors in noninbred chickens. Immunogenetics9: 97–100, 1979.
22.
CollinsWM, BrownDW, WardPH, DunlopWR, BrilesWE. MHC and non-MHC genetic influences on Rous sarcoma metastasis in chickens. Immunogenetics22: 315–321, 1985.
23.
CollinsWM. Genetic influences on the fate of RSV-induced tumors in chickens.In: Poultry Breeders of America, Eds. Proceedings of the 28th National Breeder's Roundtable. Kansas City, MO: Poultry Breeders of America, pp 1–14, 1979.
24.
LePageKT, BrilesWE, KoptiF, TaylorRLJr.Nonmajor histocompatibility complex alloantigen effects on the fate of Rous sarcomas. Poult Sci79: 343–348, 2000.
25.
DixMC, TaylorRLJr.Differential antibody responses in 6.B major histocompatibility (B) complex congenic chickens. Poult Sci75: 203–207, 1996.
26.
McGibbonWH. The genetics of green spotting on shanks of Ancona chickens. J Hered69: 97–100, 1978.
27.
BrilesWE, BrilesRW. Identification of haplotypes of the chicken major histocompatibility complex (B). Immunogenetics15: 449–459, 1982.
28.
CollinsWM, ZervasNP, UrbanWEJr., BrilesWE, AeedPA. Response of B complex haplotypes B22, B24, and B26 to Rous sarcomas. Poult Sci64: 2017–2019, 1985.
29.
ConoverWJ, ImanRL. Rank transformations as a bridge between parametric and nonparametric statistics. Am Statistician35: 124–129, 1981.
30.
TaylorRLJr., EwertDL, EnglandJM, HalpernMS. Major histocompatibility (B) complex control of the growth pattern of v-src DNA-induced primary tumors. Virology191: 477–479, 1992.
31.
CollinsWM, DunlopWR, ZsigrayRM, BrilesRW, FiteRW. Metastasis of Rous sarcoma tumors in chickens is influenced by the major histocompatibility (B) complex and sex. Poult Sci65: 1642–1648, 1986.
32.
TaylorRLJr, EnglandJM, KopenGC, ChristouAA, HalpernMS. Major histocompatibility (B) complex control of the formation of v-src-induced metastases. Virology205: 569–573, 1994.
33.
GuyrePM, ZsigrayRM, CollinsWM, DunlopWR. Major histocompatibility complex (B): effect on the response of chickens to a second challenge with Rous sarcoma virus. Poult Sci61: 829–834, 1982.
34.
PlachyJ, KarakozI, BendaV.Concomitant immunity leads to retardation or acceleration of the growth of Rous sarcomas in genetically resistant or susceptible inbred lines of chickens. Folia Biol (Praha)32: 334–348, 1986.
35.
SvobodaJ, PlachyJ, HejnarJ, KarakozI, GuntakaRV, GerykJ.Tumor induction by the LTR, v-src, LTR DNA in four B (MHC) congenic lines of chickens. Immunogenetics35: 309–315, 1992.
36.
PlachyJ, HalaK, HejnarJ, GerykJ, SvobodaJ.src-specific immunity in inbred chickens bearing v-src DNA- and RSV-induced tumors. Immunogenetics40: 257–265, 1994.
37.
VincentSC, TaylorRLJr.Virus dilution affects the anti-Rous sarcoma response of progressor but not regressor major histocompatibility (B) complex genotypes. Poult Sci67: 1491–1496, 1988.
38.
CotterPF, CollinsWM, DunlopWR, CorbettAC. The influence of thymectomy on Rous sarcoma regression. Avian Dis20: 75–78, 1976.
39.
PowellPC, HalaK, BockG, DietrichH, WickG.Immune mechanisms in Rous sarcoma regression. In: WeberWT, EwertDL Eds. Avian Immunology. New York: Alan Liss, pp 207–218, 1987.
40.
HeinzelmannEW, ZsigrayRM, CollinsWM. Cross-reactivity between RSV-induced tumour antigen and B5 MHC alloantigen in the chicken. Immunogenetics13: 29–37, 1981.
41.
HeinzelmannEW, ZsigrayRM, CollinsWM. Increased growth of RSV-induced tumours in chickens partially tolerant to MHC alloantigens. Immunogenetics12: 275–284, 1981.
42.
BrownDW, CollinsWM, WardPH, BrilesWE. Complementation of major histocompatibility haplotypes in regression of Rous sarcoma virus-induced tumors in noninbred chickens. Poult Sci61: 409–413, 1982.
43.
LukacsNW, BrilesWE, BrilesRW, TaylorRLJr.Response of major histocompatibility (B) complex haplotypes B22, B26 and B30 to Rous sarcomas. Poult Sci68: 233–237, 1989.
44.
PlachyJ.Age-dependent complementation of the B (MHC) alleles and a non-B allele controlling resistance to progression of Rous sarcomas, and genetic independence of resistance to induction of tumours in the model of inbred lines of chickens. Folia Biol (Praha)31: 284–296, 1985.
