The Cypate-Bombesin Peptide Analogue Conjugate (Cybesin) was used as a prostate tumor receptor-targeted contrast agent. The absorption and fluorescence spectra of Cybesin were measured and shown to exist in the NIR tissue “optical window”. The spectral polarization imaging of Cybesin-stained prostate cancerous and normal tissues shows that prostate cancerous tissue takes-up more Cybesin than that of prostate normal tissue, making Cybesin a potential marker of prostate cancer.
BenaronD. A.. The Future of Cancer Imaging. Cancer Metastasis Rev.21, 45–78 (2002).
3.
PetersV. G., WymanD. R., PattersonM. S., FrankG.L.. Optical Properties of Normal and Diseased Human Breast Tissues in the Visible and Near Infrared. Phys. Med. Biol.35, 1317–1334 (1990).
4.
AliJ. H., WangW. B., ZevallosM., AlfanoR. R.. Near Infrared Spectroscopy and Imaging to Probe Differences in Water Content in Normal and Cancer Human Prostate Tissues. Technol Cancer Res Treat3, 491–497 (2004).
5.
BugajJ. E., AchilefuS., DorshowR. B., RajagopalanR.. Novel Fluorescent Contrast Agents for Optical Imaging of In Vivo Tumor Based on a Receptor-targeted Dye-peptide Conjugate Platform. J Biomed Opt6, 122–133 (2001).
6.
KaiL., RiefkeB., NtziachristosV., BeckerA., ChanceB., SemmlerW.. Hydrophilic Cyanine Dyes as Contrast Agents for Near-infrared Tumor Imaging: Synthesis, Photophysical Properties, and Spectroscopic In Vivo Characterization. Photochem Photobiol.72, 392–398 (2002).
7.
WangW., AliJ. H., AlfanoR. R., VitensonJ. H., LombardoJ. M.. Spectral Polarization Imaging of Human Rectum-Membrane-Prostate Tissues. IEEE Journal of Selected Topics in Quantum Electronics9, 288–293 (2003).
HaleG. M., QuerryM. R.. Optical Constants of Water in the 200-nm to 200-Mm Wavelength Region. Applied Optics12, 555–563 (1973).
10.
GoldsmithS. J.. Receptor Imaging: Competitive or Complementary to Antibody Imaging. Semin. Nucl. Med.27, 85–93 (1997).
11.
AchilefuS., DorshowR. B., BugajJ. E., RajagopalanR.. Novel Receptor-targeted Fluorescence Contrast Agent for In Vivo Tumor Imaging. Investigative Radiology35, 479–485 (2000).
12.
ReubiJ. C., WengerS., Schmuckli-MaurerJ., SchaerJ.-C., GuggerM.. Bombesin Receptor Subtypes in Human Cancers: Detection with the Universal Radioligand (125)I-(D-TYR(6), beta-ALA(11), PHE(13), NLE(14)) bombesin(6–14). Clin Cancer Res8, 1139–1146 (2002).
13.
LangerM., Beck-SickingerA. G.. Peptides as Carrier for Tumor Diagnosis and Treatment. Curr Med Chem Anti-Canc Agents1, 71–93 (2001).
14.
GotsisE. D.. In Vivo Proton MR Spectroscopy of Brain Lesions. Institute of High Medical Technology EUROMEDICA, Advanced Research and Therapeutic Institute ENCEPHALOS. http://users.mland.gr/sgotsis/spectroscopy/mrs.html.
15.
WangW. B., DemosS. G., AliJ., ZhangG., AlfanoR. R.. Visibility Enhancement of Fluorescent Objects Hidden in Animal Tissue Using Spectral Fluorescence Difference Method. Optical Communications147, 11–15 (1998).
