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Abstract

Background

Prediabetes (PDM) and diabetes mellitus (DM) are common among acute coronary syndrome (ACS) patients. The present study evaluated the association between diabetes status and radial artery (RA) atherosclerosis using optical coherence tomography (OCT) in ACS patients.

Methods

A total of 335 ACS patients who underwent RA OCT were categorized into the DM group, the PDM group, and the normal glucose metabolism (NGM) group. OCT characteristics and clinical variables were compared.

Results

RA atherosclerotic plaques were more frequent in the PDM and DM groups than in the NGM group (38.7% vs. 33.3% vs. 16.1%, p = 0.001). Lipid and calcified plaque occurrence were significantly more common in the DM group, followed by the PDM and NGM groups (19.3% vs. 14.6% vs. 6.5%, p = 0.027; 11.8% vs. 6.5% vs. 1.1%, p = 0.009). The prevalence of microvessels in the PDM group was significantly higher (42.7% vs 23.7%, p = 0.017) than in the NGM group but was comparable to the DM group. Multivariate analysis revealed that HbA1c level and age were independent predictors of RA plaque formation and eccentric intimal hyperplasia (all p<0.05).

Conclusions

RA atherosclerosis characteristics differ according to diabetes status. HbA1c level could be a useful marker for RA atherosclerosis progression in ACS patients.

Keywords

Diabetes prediabetes optical coherence tomography acute coronary syndrome radial artery atherosclerosis

Key messages

- Patients with DM and PDM were more likely to have RA atherosclerosis than those with normal glucose levels.

- RA OCT characteristics differ according to glycemic status.

- HbA1c level may be a useful marker for RA atherosclerosis progression in patients with ACS.

Introduction

Cardiovascular disease is the leading cause of death in patients with diabetes mellitus (DM), and approximately one-fourth of patients who develop acute coronary syndrome (ACS) have DM.¹ Coronary artery bypass grafting (CABG) has been demonstrated to improve long-term outcomes after ACS in DM patients with multivessel coronary disease.^2-4 The radial artery (RA) is considered as a second arterial conduit for CABG due to its efficacy and safety.⁵ However, previous studies demonstrated that radial arteries are associated with some degree of atherosclerosis which may have a detrimental effect on graft patency or render them unsuitable as a graft in CABG.^6-8 Although DM is a known risk factor for silent RA atherosclerosis,^6,9,10 the incidence of RA atherosclerosis in patients with DM has not been widely investigated. Several methods have been used to evaluate RA atherosclerosis including ultrasonography,¹¹ intravascular ultrasound (IVUS),¹² and harvested radial artery pathology,⁶ but their role in detecting atherosclerosis is limited by their poor spatial resolution or presence of pathology in the discarded distal segments of the RA.

Optical coherence tomography (OCT) is a high-resolution imaging technique that has been well established for evaluating vulnerable coronary plaques in patients with ACS.¹³ Its utility for the evaluation of RA atherosclerotic plaque was confirmed by a previous validation study in which RA atherosclerotic plaque components and tissue layers were identified by OCT and which reported high agreement with histological results.¹⁴

In this study, we compared RA atherosclerosis among diabetic and non-diabetic patients with ACS using OCT. We also investigated whether DM duration had any negative effects on plaque characteristics in diabetic ACS patients.

Methods

Patient population

A total of 462 consecutive patients with ACS who underwent RA OCT after coronary OCT at our institute between March 2019 and December 2020 were enrolled in the present study. The study flowchart is shown in Figure 1. Clinical data such as age, sex, and classic risk factors for atherosclerosis were collected. The study population was stratified according to three glycemic categories in accordance with the definition by the American Diabetes Association¹⁵: DM group [(self-reported type 2 DM, hemoglobin A1c (HbA1c) >6.4% (47 mmol/mol), and/or use of insulin)], prediabetes group [(PDM, no self-reported type 2 DM and HbA1c of 5.7%–6.4% (39–47 mmol/mol)], and normoglycemia group [NGM, no self-reported type 2 DM and HbA1c < 5.7% (39 mmol/mol)].¹⁶ The HbA1c levels in whole blood were routinely measured in all patients using high-performance liquid chromatography with ELUENT 80A (ARKRAY, IN, Japan). Patients with type 2 DM were further divided into two subgroups: long-DM group (DM duration ≥ 10 years) and short-DM group (DM duration <10 years). All laboratory data were collected during fasting throughout the hospitalization period. Written informed consent was obtained from all the patients. This study was performed in accordance with the Declaration of Helsinki and was approved by the ethics committee of the Beijing Luhe Hospital, Capital Medical University (Beijing, China).

Figure 1.

Study flowchart.

OCT image acquisition

After a 6F sheath (Terumo Co, Tokyo, Japan) was inserted at the default vascular access of the right RA, coronary angiography and percutaneous coronary intervention were performed with 6 Fr catheters. Upon completion of the coronary procedure, the protocol described below (Figure 2) was implemented to perform OCT imaging of the RA. Three OCT pullback recordings (20 mm/s) including proximal (0–50 mm), middle (50–100 mm), and distal (100–150 mm) portions were acquired using saline to flush the RA. The OCT procedure was performed using a C7XR FD-OCT system (St. Paul, MN, USA).

Figure 2.

Algorithm for radial artery OCT imaging. OCT = optical coherence tomography; RA = radial artery; RAA = radial artery angiography.

OCT image interpretation

The OCT image analysis was performed using proprietary software (LightLab Imaging), and the correct calibration settings for Z-offset were confirmed by two independent and experienced readers (TZ, LZX) who were unaware of the clinical data.

The RA imaging characteristics were based on the current OCT consensus standard.¹⁷ In this study, atherosclerotic plaques were defined and classified into three categories¹⁷: (1) fibrous plaques were defined by a homogeneous signal and high backscattering region; (2) lipid plaques were defined by a low-signal region with a diffuse border; and (3) calcified plaques were defined as a plaque containing calcium deposits (signal-poor regions with sharply delineated borders). Representative images are shown in Figure 3. The longitudinal length of each plaque was assessed, and two plaques were seen as separated when their distance was more than 10 mm in the longitudinal view. For lipid plaques, the fibrous cap thickness (FCT) and maximal lipid arc were measured three times at the thinnest part, and the average value was calculated. When lipid deposits were present at ≥ 90 in any of the cross-sectional images within the plaque, it was considered a lipid-rich plaque. A TCFA was defined as an FCT thickness of ≤65 μm in a lipid-rich plaque on cross-sectional imaging.¹⁸ Calcium deposits were analyzed individually by measuring calcium longitudinal length, arc, and depth. The maximum calcium arc, maximal depth, and FCT of the calcific plaques were analyzed. Spotty calcification was defined plaques with a length of <4 mm and a maximal arc of <90°. Microvessels were defined as black holes with diameters of 50–300 μm that were sharply delineated for at least three consecutive frames. Macrophage infiltration was defined as signal-rich, distinct, or confluent punctuate regions that exceed the intensity of background speckle noise.¹⁷ The severity of intimal hyperplasia in RA has been reported previously^6,10,19 (Supplemental Methods).

Figure 3.

Representative cross-sectional optical coherence tomography images. (A) Normal RA wall shows a 3-layered architecture. (B) The microvessel (arrow) present in a patient with PDM. (C) The macrophage (arrows) present in a DM patient with long disease duration. (D) Fibrous plaque identified as a homogeneous, highly backscattering region (arrow). (E) The representative case of lipid-rich plaque. The fibrous cap thickness overlying a lipid-rich plaque was 80 μm at its thinnest part (arrow). (F) Calcified plaque identified by the presence of a well-delineated, low-backscattering heterogeneous region (asterisk). DM = diabetes mellites; NGM = normoglycemia; PDM = prediabetes.

Reproducibility of qualitative OCT assessment

An author (TZ) who was blinded to clinical, angiographic, and OCT data selected 50 cases after all annotations on OCT images were deleted. For the inter-observer variability assessment, two experienced OCT observers (LZX and TZ) interpreted the 50 OCT cases, and then an OCT observer (LZX) assessed them again at 4-week intervals. The inter- and intra-observer kappa coefficients for the plaque presence and plaque phenotypes were 0.919∼0.975.

Statistical analysis

Statistical analysis was performed using SPSS (version 23.0; IBM Corp., Armonk, New York). Categorical variables were expressed as numbers (percentages). Continuous variables were presented as mean ± standard deviation. The normality of variables was assessed using the Shapiro–Wilk test. Categorical variables were compared using the x² test. Continuous variables were compared using the Mann–Whitney U test, one-way analysis of variance (ANOVA), or Kruskal–Wallis ANOVA on ranks as appropriate. Further multiple comparison analysis was performed to analyze within-group differences; the post-hoc Scheffe method was chosen for continuous data, and the Bonferroni method for categorical data. The association between RA plaque presence and traditional risk factors or biochemical biomarkers was assessed using multivariate analysis. All variables with p < 0.1 in univariable analysis were entered en bloc in the multivariable model. Variables with significant contribution to the regression model were kept in the combination models and were plotted with a receiver-operating characteristic (ROC) curve. A two-tailed p value of <0.05 was considered statistically significant.

Results

Study populations and baseline characteristics

The patient characteristics are summarized in Table 1. Of the enrolled patients, 119 (35.5%) had DM, 123 (36.7%) had PDM, and 93 (27.8%) had normoglycemia. Patients with DM were older, more likely to be female, had a higher prevalence of hypertension and higher body mass index, and had higher estimated glomerular filtration rate (eGFR) and fasting plasma glucose level. However, other risk factors were equally distributed among the three groups. Among the 119 patients with DM, 42 were newly diagnosed at admission. Concerning antihyperglycemic therapies, 18 patients were treated with insulin, 55 with oral agents, and 8 with diet modification alone.

Table 1.

Baseline patient characteristics.

Variable	Overall (n = 335)	NGM (n = 93)	PDM (n = 123)	DM (n = 119)	P value
Age, years	57.47±13.20	53.55±13.12	58.89±13.47^a	59.08±12.43^b	0.003
Male	264 (78.8)	78 (83.9)^c	101 (82.1)	85 (71.4)^b	0.047
BMI, kg/m²	26.22±3.41	25.42±3.45	26.49±3.44	26.56±3.26^b	0.029
Smoking	221 (66.0)	69 (74.2)	81 (65.9)	71 (59.7)	0.086
Hypertension	179 (53.4)	39 (41.9)	64 (52.0)	76 (63.9)^b	0.006
Hyperlipidemia	194 (62.0)	50 (55.6)	73 (64.6)	71 (64.5)	0.331
PVD	9 (2.7)	2 (2.2)	2 (1.6)	5 (4.2)	0.444
Renal insufficiency	18 (5.4)	3 (3.2)	7 (5.7)	8 (6.7)	0.497
Prior stroke	22 (6.6)	7 (7.5)	6 (4.9)	9 (7.6)	0.636
Prior MI	3 (0.9)	1 (1.1)	0	2 (1.7)	0.386
CAD family history	43 (12.8)	11 (11.8)	16 (13.0)	16 (13.4)	0.938
Indication for angiography					0.266
STEMI	231 (69.0)	69 (74.2)	88 (71.5)	74 (62.2)
NSTEMI	66 (19.7)	14 (15.1)	21 (17.1)	31 (26.1)
UAP	38 (11.3)	10 (10.8)	14 (11.4)	14 (11.8)
Triple vessel disease	98 (29.3)	25 (26.9)	27 (22.0)	46 (38.7)^c	0.014
Multivessel disease	212 (63.3)	51 (54.8)	75 (61.0)	86 (72.3)^b	0.026
Biochemistry data
HbA1c, %	6.66±1.80	5.42±0.02	5.96±0.02^a	8.34±0.20^b,c	<0.001
Fasting glucose, mmol/L	6.00 (5.17±7.60)	5.14 (4.69±5.80)	5.75 (5.24±6.5)^a	8.39 (6.45±10.74)^b,c	<0.001
Triglycerides, mg/dL	150.43±116.06	138.89±96.35	138.95±71.39	171.32±157.91	0.050
TC, mg/dL	177.40±41.48	172.82±41.57	183.23±41.60	174.95±40.93	0.137
LDL-C, mg/dL	116.85±34.32	115.13±36.53	121.10±34.46	113.79±32.17	0.216
HDL-C, mg/dL	42.42±13.17	43.70±2.12	42.08±8.31	41.78±9.51	0.536
Lipoprotein (a), mg/L	125 (68-261)	109 (71,247)	132 (71,285)	126 (56,245)	0.706
Creatinine, μmmol/L	75.79±15.88	75.41±15.62	77.61±15.02	74.20±16.87	0.240
eGFR, mL/min/1.73 m²	91.77±17.41	95.50±17.49^a	89.70±16.52	90.99±17.92	0.044
Hemoglobin, g/L	141.33±15.96	142.36±17.59	140.52±14.32	141.37±16.32	0.704
Procedures					0.308
CAG	29 (8.7)	11 (11.8)	11 (8.9)	7 (5.9)
PCI	306 (91.3)	82 (88.2)	112 (91.1)	112 (94.1)
Procedure time, min	78.11±28.65	75.40±22.91	80.52±31.16	77.73±29.98	0.424
Volume of CM, mL	203.18±69.88	200.69±55.25	204.62±72.02	203.64±77.93	0.917
Prior medications
Antiplatelet	13 (3.9)	3 (3.2)	4 (3.3)	6 (5.0)	0.772
Statin	16 (4.8)	3 (3.2)	4 (3.3)	9 (7.6)	0.224
CCB	71 (21.2)	18 (19.4)	23 (18.7)	30 (25.2)	0.407
Beta blocker	22 (6.6)	3 (3.2)	8 (6.5)	11 (9.2)	0.214
ACEI/ARB	44 (13.1)	11 (11.8)	14 (11.4)	19 (16.0)	0.520
Insulin				10 (8.4)
Oral agents				47 (39.5)
Diet only				8 (6.7)
Newly diagnosed DM				42 (12.5)
Duration of DM				7.58±5.81
Short (<10 years)				78
Long (≥10 years)				41

Data are shown as mean± standard deviation, number (%), or median (interquartile range).

ACE = angiotensin-converting enzyme; ARB = angiotensin II receptor blocker; CAD = coronary artery disease; CAG = coronary angiogram; CCB = calcium channel blocker; DM = diabetes mellites; HDL-C = high-density lipoprotein cholesterol; LDL = low-density lipoprotein; MI = myocardial infarction; NGM = normoglycemia; PDM = prediabetes; PCI = percutaneous coronary intervention; renal insufficiency = estimated glomerular filtration <60 mL/min/1.73 m²; VD = vessel disease; SD = standard deviation.

P<0.05 for.

NGM group versus PDM group.

NGM group versus DM group.

PDM group versus DM group.

OCT findings

A comparison of the OCT findings among the three groups is shown in Table 2. In patient-level analysis, RA plaques were more prevalent in patients with DM and PDM than in those with NGM (38.7% vs. 33.3% vs. 16.1%, p = 0.001). The DM and PDM groups demonstrated higher plaque numbers per patient than the NGM group (2.26±1.06 and 1.88±1.12 vs 1.50±0.82, p = 0.035). The proportion of RA lipid plaques was significantly lower in the NGM group than in the DM group (19.3% vs. 6.5%, p = 0.007). However, patients in the DM group had fewer lipid plaques than those in the PDM group, with a borderline significance (14.6% vs. 6.5%, p = 0.058). Calcified plaques were only detected in one patient in the NGM group (1.1%), whereas the incidence of calcified plaques was comparable between the DM and PDM groups (11.8% and 6.5%, respectively; p = 0.155). In particular, calcified plaques were prone to cluster distally in the RA of DM patients (10.1%). There were no significant differences in fibrous plaques among the three groups. In addition, the percentage of microvessels was significantly higher in the DM group (42.7% vs. 23.7%, p = 0.002) and PDM groups (39.0% vs. 23.7%, p = 0.017) than in the NGM group.

Table 2.

Optical coherence tomography features of the RA stratified according to glycemic status.

Variable	Total	NGM (n = 93)	PDM (n = 123)	DM (n = 119)	p value
RA length^a, mm	171.98±16.90	172.10±16.59	172.95±16.58	170.89±17.54	0.641
≥1 Plaque	102 (30.4)	15 (16.1)	41 (33.3)^b	46 (38.7)^c	0.001
Plaques number per patient	1.99±1.08	1.50±0.82	1.88±1.12	2.26±1.06^c	0.035
Total plaque length	8.05 (4.35–17.73)	6.6 (3.3–9.6)	7.4 (2.95–14.65)	8.95 (4.80–32.45)	0.151
Plaque location
Proximal	59 (17.6)	7 (7.5)	22 (17.9)^b	30 (25.2)^c	0.004
Middle	42 (12.5)	6 (6.5)	6 (13.0)	20 (16.8)^c	0.076
Distal	71 (21.2)	9 (9.7)	26 (21.1)^b	36 (30.3)^c	0.001
Fibrous plaque	68 (20.3)	11 (11.8)	28 (22.8)	29 (24.4)	0.055
Total length	5.30 (2.73–9.40)	4.9 (3.0–9.6)	5.20 (2.18–7.95)	5.6 (2.8–17.65)	0.564
Lipid plaque	47 (14.0)	6 (6.5)	18 (14.6)	23 (19.3)^c	0.027
Total length	5.60 (3.20–10.80)	5.05 (2.68–8.55)	5.7 (4.4–14.3)	4.8 (3.2–10.8)	0.504
Calcified plaque	23 (6.9)	1 (1.1)	8 (6.5)	14 (11.8)^c	0.009
Total length	9.8 (5.9–35.0)	2.9	9.05 (6.50–47.95)	10.70 (4.83–39.83)	0.375
Micro vessels	122 (36.4)	22 (23.7)	48 (39.0)^b	52 (42.7)^c	0.008
Macrophages	43 (12.8)	8 (8.6)	15 (12.2)	20 (16.8)	0.201
RA structures
Lumen area, mm²	6.22 (5.00–8.00)	6.15 (4.52–7.78)	6.58 (5.11–8.13)	5.94 (4.90–8.11)	0.332
Diameter, mm	2.80 (2.52–3.18)	2.78 (2.43–3.11)	2.86 (2.54–3.21)	2.75 (2.52–3.21)	0.242
Maximal intimal thickness, mm	0.13 (0.08–0.17)	0.10 (0.06–0.14)	0.13 (0.08–0.18)^b	0.15 (0.10–0.19)^c	<0.001
IMR	0.90 (0.53–1.40)	0.75 (0.37–1.20)	0.91 (0.53–1.27)	1.10 (0.65–1.63)^c	0.001
IEI	2.65 (1.87–3.25)	2.19 (1.67–2.92)	2.72 (1.83–3.24)^b	2.84 (2.17–3.44)^c	<0.001
ITI	0.46 (0.37–0.57)	0.43 (0.36–0.50)	0.46 (0.37–0.58)	0.49 (0.38–0.60)	0.217
LN, %	26.95 (23.92–30.45)	26.11 (23.48–29.67)	27.26 (24.22–30.39)	27.56 (23.92–31.32)	0.622
Intima area, mm²	0.72 (0.55–0.93)	0.63 (0.49–0.85)	0.74 (0.58–0.95)^b	0.76 (0.59–0.96)^c	0.005
Media area, mm²	1.60 (1.32–1.91)	1.54 (1.23–1.92)	1.64 (1.36–1.91)	1.62 (1.36–1.91)	0.790
Crescent-shaped intimal hyperplasia	53 (15.8)	8 (8.6)	19 (15.4)	26 (21.8)^c	0.032

IMR = intima-media ratio; IEI = intimal eccentricity index; ITI = intimal thickness index; LN = luminal narrowing.

Distance from RA ostium to puncture site;

p < 0.05 for NGM group versus PDM group;

p < 0.05 for NGM group versus DM group

Among DM patients, the frequency of the three plaque phenotypes and macrophage presence was higher in the long-DM group than in the short-DM group (all p < 0.05) (Table 3).

Table 3

Subgroup analysis of DM (n = 119)

Variable	DM duration <10 years (n = 78)	DM duration ≥10 years (n = 41)	p value
Presence of plaque	21(26.9)	25(61.0)	<0.001
Plaque site
Proximal	13(16.7)	17(41.5)	0.003
Middle	7(9.0)	13(31.7)	0.002
Distal	15(19.2)	21(51.2)	<0.001
Total length	7.90(4.80–13.30)	13.10(4.80–36.65)	0.270
Fibrous	14(17.9)	15(36.6)	0.024
Total length	4.9(2.85–8.65)	6.60(2.30–26.50)	0.541
Lipid	10(12.8)	13(31.7)	0.013
Total length	4.85(3.05–14.45)	4.80(2.90–11.60)	0.877
Calcification	5(6.4)	9(22.0)	0.028
Total length	10.10(6.10–51.45)	13.40(5.65–44.65)	0.841
Micro vessels	32(41.0)	20(48.8)	0.418
Macrophages	9(11.5)	11(26.8)	0.034
Crescent-shaped intimal hyperplasia	18(23.1)	8(19.5)	0.655

In plaque-level analysis, a total of 205 RA plaques were detected in 102 cases (24 plaques in 15 NGM subjects, 77 plaques in 41 PDM subjects, and 104 plaques in 46 DM subjects). A comparison of the quantitative OCT findings at the plaque level among the three groups is presented in Supplementary Table 1.

As shown in Table 2 and Supplementary Table 2, compared with the NGM group, the DM and PDM groups showed a significant increase in intima-media ratio (IMR), maximum intimal thickness (MIT), intimal eccentricity index (IEI), and intimal area (all p < 0.05). The proportion of crescent-shaped intimal hyperplasia was the highest in diabetic patients, followed by that in prediabetic and NGM patients, respectively (21.8% vs. 15.4% vs. 8.6%, p = 0.032).

Multivariate analysis of risk factors for RA plaque formation

The findings of univariate and multivariate logistic regression analyses using RA plaque presence as the dependent variable are shown in Table 4 and Supplementary Table 4. All the variables in univariate logistic regression analysis with p < 0.1 were enter en bloc in the multivariable model, including age, sex, hypertension, eGFR, triglyceride levels, prior antiplatelet and history of statin use. Multivariate analysis showed that HbA1c level and age were identified as independent risk factors for RA plaque formation (all p < 0.05). The area under the ROC curve of HbA1c and age for RA plaque presence was 0.779 (95% CI 0.727–0.832, p < 0.001), with sensitivity and specificity of 71.6% and 65.1%, respectively (Figure 4). Longer duration (≥10 years) and age were independent risk factors for RA plaque in patients with DM (Table 4 and Supplementary Table 3).

Table 4

Logistic regression analysis of RA plaque

Variable	Univariate analysis			Multivariate analysis
	p value	OR	95%CI	p value	OR	95%CI
For Total ^a
Age, years	<0.001	1.086	1.061–1.111	<0.001	1.087	1.053–1.122
HbA1c, %	0.029	1.148	1.014–1.299	0.007	1.221	1.056–1.411
For DM ^b
Age, years	<0.001	1.093	1.050–1.137	<0.001	1.099	1.043–1.159
DM Duration (long vs. short)	<0.001	4.241	1.901–9.463	0.005	3.752	1.502–9.369

^aadjustment for age, sex, hypertension, eGFR, HbA1c, triglycerides, statin, and antiplatelet.

^badjustment for age, DM duration, eGFR, hypertension, and triglycerides.

Figure 4.

Receiver-operating characteristic curves of HbA1c combined with age for predicting RA plaque presence.

Multivariate linear regression analysis showed that HbA1c level was the only independent predictor of IEI after controlling for age (Table 5 and Supplementary Table 5).

Table 5

Linear regression analysis of RA intimal hyperplasia

Variable	Univariate analysis			Multivariate analysis
Variable	β	p Value	95% CI for β	β	p Value	95% CI for β
IEI ^a
Age, years	0.031	<0.001	0.024–0.039	0.413	<0.001	0.024–0.039
HbA1c, %	0.143	0.009	0.020–0.138	0.135	0.007	0.021–0.128
IMR ^b
Age, years	0.541	<0.001	0.024–0.034	0.491	<0.001	0.021–0.032
Sex	−0.317	<0.001	−0.731–0.375	−0.131	0.009	−0.397–0.058

^aadjustment for sex, smoker, BMI, eGFR, hypertension, familial history of CAD, lipid levels, cholesterol, and medications.

^badjustment for smoker, BMI, eGFR, HbA1c, hypertension, familial history of CAD, lipid levels, cholesterol, and medications.

Discussion

In the present study, OCT, a novel high-resolution technique with a spatial resolution of 10 μm, demonstrated that atherosclerotic plaque presence and the extent of intimal hyperplasia of the RA in the PDM group were greater than those in the NGM group but comparable to those in the DM group. HbA1c levels increase in advanced atherosclerosis and can help identify at-risk individuals. Moreover, individuals with a longer duration of DM showed a higher prevalence of RA plaque phenotypes and macrophage presence. These data suggest that even prediabetic status affects the microstructure of the RA; meanwhile, a longer duration of DM will further aggravate atherosclerosis.

Evidence suggests that patients with DM have accelerated vascular atherosclerosis that affects multiple vessels,²⁰ and prolonged exposure to high levels of glucose can alter systemic endothelial function.²¹ In line with previous studies, DM was identified as a risk factor for RA intimal hyperplasia or atherosclerosis.^6,9,12 Electron microscopic comparisons of distal RA grafts obtained from diabetic patients have previously shown an increase in histological changes, such as endothelial cell damage, compared to grafts from non-diabetic patients, especially those with long-standing duration or poor glycemic control.²² Recent studies have reported that patients with PDM have a higher prevalence of subclinical atherosclerosis.²³ Thus, our study raises the question of the subclinical status of RA structural changes that occur before and after the diagnosis of DM. Structures such as arterial layers and plaque components of the RA are often difficult to visualize in vivo and are easily evaluated by OCT after routine coronary procedures.

Prior work has documented that preoperative quality evaluation of RA grafts is crucial for their long-term patency.²⁴ For example, ultrasound¹¹ and histopathologic⁹ studies have demonstrated that RAs in diabetic patients were more prone to calcium deposition, which may make the RA an unusable conduit in many of these patients.²⁵ Brown et al.²⁶ confirmed that intraoperative OCT imaging of RAs that were considered as acceptable for bypass grafting allowed for detection of intimal lipids which could help predict the degree of postoperative spasm of the RA. Importantly, the current OCT observation expands this understanding; we found a significantly higher incidence of RA plaque presence in both the DM and PDM groups than in the NGM group, and the incidence of RA lipid plaque was significantly higher in the DM group, followed by that in the PDM and NGM groups. Given the complex abnormalities of vascular complications seen in DM patients, it is not surprising that percutaneous or surgical coronary revascularization after ACS is less effective in diabetic than in non-diabetic patients.^27-29

Jared et al. reported that the durations of DM and PDM during adulthood are both independently associated with subclinical atherosclerosis in middle-aged patients.³⁰ In the present study, patients with long-standing DM had higher levels of RA atherosclerotic alterations, including plaque phenotype and macrophage presence, which may aggravate its impaired vasoreactivity.¹¹ These findings add to the relationship between HbA1c level, disease duration, and diabetic atherosclerosis progression in ACS patients, which helps to understand the structural alterations of the vascular tree in patients with DM.

Pathologic studies revealed that coronary tissue from patients with DM exhibited more microstructure than that from patients without DM.³¹ Microvessels are focally enhanced in areas of increased vessel wall thickness, and atherosclerotic lesion formation typically precedes the appearance of endothelial dysfunction.³² Consistently, we found a significant increasing trend in RA microvessel presence among patients with NGM, PDM, and DM. Furthermore, macrophage accumulation was more common in patients with longer DM duration, which has been reported to play an important role during various steps of atherosclerosis and promote ACS events, thus indicating a higher level of plaque vulnerability.³³

HbA1c is not only a useful biomarker of long-term glycemic control but also a good predictor of atherosclerosis. For example, Shah et al.³⁴ reported that a 1% increase in HbA1c is associated with an approximately 30% increase in probability of a thicker carotid IMT. Recently, Rossello et al.³⁵ demonstrated a linear association between HbA1c > 5.4% level and multi-territory subclinical atherosclerosis detected by vascular ultrasound and non-contrast cardiac CT in carotid arteries, coronary arteries, and peripheral arteries in non-diabetic individuals. In the present study, we found an obvious intimal hyperplasia of the RA in the DM and PDM groups but not in the NGM group, and the intimal eccentric index was positively associated with HbA1c level. These findings extend previous research on the effect of HbA1c and highlight the association between HbA1c and RA atherosclerosis.

Data obtained in previous studies using a high-frequency ultrasound (55 Hz) indicated that assessment of RA intima thickness, other than RA media thickness, provides a tool for non-invasive early detection of atherosclerosis or additive value for the diagnosis of coronary artery disease.^36,37 Although IVUS has been used to quantify RA atheroma burden, it cannot reliably identify the separation between the intima and media because of its limited spatial resolution.³⁸ In our study, the use of OCT allowed for the evaluation of even slight metabolic changes which contribute to the microstructural phenotype changes of the RA. The potential of RA intimal hyperplasia or plaque components that considered as cardiovascular risk modifiers deserve further investigation in future studies.

Study limitations

The present study had some limitations. First, this was a retrospective observational study from a single-center database; therefore, selection bias may have influenced the results. Second, we could not confirm that the effects of anti-atherosclerotic drugs, such as statins and hypoglycemic agents, were not independently associated with plaque regression. Recent studies have demonstrated that statin therapy increases FCT, thereby stabilizing the plaque irrespective of the DM status.³⁹ Third, the lack of longitudinal follow-up data did not allow for assessment of the clinical impact of OCT findings on future events. Fourth, in the present study, although it is hypothesized that the region of crescent-shaped intimal hyperplasia is prone to plaque formation, these data were not examined. Fifth, the RA is a good candidate for OCT imaging owing to its diameter; however, OCT is an invasive method used in the research stage and its role in clinical applications has not been fully established. In the present study, OCT imaging of the RA required little additional effort after the completion of the coronary OCT procedure during transradial intervention. In addition, to avoid wire artifacts, when the OCT catheter was in place, the guide wire was removed from the body. In the future, non-invasive and very high-resolution ultrasound is needed to evaluate RA atherosclerosis in clinical scenarios.

Conclusions

PDM stratified by HbA1c level is associated with RA plaque characteristics and eccentric intimal hyperplasia in ACS patients, similar to DM. HbA1c level could be a useful marker for RA atherosclerosis progression. Moreover, DM with a longer duration contributed to more damage to RA. This study highlighted that the prevention of vascular complications started below the threshold for DM, and the RA in patients with extended DM duration requires more attention.

Supplemental Material

sj-pdf-1-dvr-10.1177_14791641221078108 – Supplemental Material for Impact of prediabetes and duration of diabetes on radial artery atherosclerosis in acute coronary syndrome patients: An optical coherence tomography study

Supplemental Material, sj-pdf-1-dvr-10.1177_14791641221078108 for Impact of prediabetes and duration of diabetes on radial artery atherosclerosis in acute coronary syndrome patients: An optical coherence tomography study by Zixuan Li, Zhe Tang, Yujie Wang, Zijing Liu, Senhu Wang, Yuntao Wang, Guozhong Wang, Yuping Wang and Jincheng Guo in Diabetes & Vascular Disease Research

Footnotes

Declaration of conflicting interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by grant No.2018-2-7082 from the Capital’s Funds for Health Improvement and Research, and grant No.2020CX004-15 from the TongZhou District Funds.

ORCID iD

Zixuan Li

Supplemental material

Supplemental material for this article is available online.

Appendix

References

Farkouh

Domanski

Dangas

, et al. Long-term survival following multivessel revascularization in patients with diabetes: The FREEDOM follow-on study. J Am Coll Cardiol 2019; 73: 629–638.

Godoy

Tavares

CAM

Farkouh

. Weighing coronary revascularization options in patients with type 2 diabetes mellitus. Can J Diabetes 2020; 44: 78–85.

Godoy

Rao

Farkouh

. Coronary revascularization of patients with diabetes mellitus in the setting of acute coronary syndromes. Circulation 2019; 140: 1233–1235.

Ramanathan

Abel

Park

, et al. Surgical versus percutaneous coronary revascularization in patients with diabetes and acute coronary syndromes. J Am Coll Cardiol 2017; 70: 2995–3006.

Gaudino

Benedetto

Fremes

, et al. Association of radial artery graft vs saphenous vein graft with long-term cardiovascular outcomes among patients undergoing coronary artery bypass grafting: A systematic review and meta-analysis. JAMA 2020; 324: 179–187.

Ruengsakulrach

Sinclair

Komeda

, et al. Comparative histopathology of radial artery versus internal thoracic artery and risk factors for development of intimal hyperplasia and atherosclerosis. Circulation 1999; 100: II139–44.

Tinica

Vartic

Mocanu

, et al. Preoperative graft assessment in aortocoronary bypass surgery. Exp Ther Med 2016; 12: 804–808.

Gaudino

Antoniades

Benedetto

, et al. Mechanisms, consequences, and prevention of coronary graft failure. Circulation 2017; 136: 1749–1764.

Chowdhury

Airan

Mishra

, et al. Histopathology and morphometry of radial artery conduits: basic study and clinical application. Ann Thorac Surg 2004; 78: 1614–1621.

10.

Kaufer

Factor

Frame

, et al. Pathology of the radial and internal thoracic arteries used as coronary artery bypass grafts. The Ann Thorac Surg 1997; 63: 1118–1122.

11.

Nicolosi

Pohl

Parsons

, et al. Increased incidence of radial artery calcification in patients with diabetes mellitus. J Surg Res 2002; 102: 1–5.

12.

Moon

Kim

Yoo

, et al. Evaluation of radial artery atherosclerosis by intravascular ultrasound. Angiology 2013; 64: 73–79.

13.

Prati

Guagliumi

Mintz

, et al. Expert review document part 2: methodology, terminology and clinical applications of optical coherence tomography for the assessment of interventional procedures. Eur Heart J 2012; 33: 2513–2520.

14.

Brown

Burris

, et al. Thinking inside the graft: Applications of optical coherence tomography in coronary artery bypass grafting. J Biomed Opt 2007; 12: 051704.

15.

American Diabetes Association . 2. Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes-2021. Diabetes Care 2021; 44: S15–S33.

16.

Honigberg

Zekavat

Pirruccello

, et al. Cardiovascular and kidney outcomes across the glycemic spectrum: insights from the UK Biobank. J Am Coll Cardiol 2021; 78: 453–464.

17.

Tearney

Regar

Akasaka

, et al. Consensus standards for acquisition, measurement, and reporting of intravascular optical coherence tomography studies: A report from the international working group for intravascular optical coherence tomography standardization and validation. J Am Coll Cardiol 2012; 59: 1058–1072.

18.

Zhang

Dai

Jia

, et al. Non-culprit plaque characteristics in acute coronary syndrome patients with raised hemoglobinA1c: An intravascular optical coherence tomography study. Cardiovascular Diabetology 2018; 17: 90.

19.

Yonetsu

Kakuta

Lee

, et al. Assessment of acute injuries and chronic intimal thickening of the radial artery after transradial coronary intervention by optical coherence tomography. Eur Heart J 2010; 31: 1608–1615.

20.

Gao

Song

Watase

, et al. Differences in carotid plaques between symptomatic patients with and without diabetes mellitus. Atvb 2019; 39: 1234–1239.

21.

Schalkwijk

Stehouwer

. Vascular complications in diabetes mellitus: The role of endothelial dysfunction. Clin Sci (Lond) 2005; 109: 143–159.

22.

Gursoy

Guzel

Erturkuner

, et al. Electron microscopic comparison of radial artery grafts in non-diabetic and diabetic coronary bypass patients. J Card Surg 2016; 31: 410–415.

23.

McNeely

McClelland

Bild

, et al. The association between A1C and subclinical cardiovascular disease: The multi-ethnic study of atherosclerosis. Diabetes Care 2009; 32: 1727–1733.

24.

Vukovic

Peric

Radak

, et al. Preoperative insight into the quality of radial artery grafts. Angiology 2017; 68: 790–794.

25.

Stahli

Caduff

Greutert

, et al. Endothelial and smooth muscle cell dysfunction in human atherosclerotic radial artery: Implications for coronary artery bypass grafting. J Cardiovasc Pharmacol 2004; 43: 222–226.

26.

Brown

Burris

Kon

, et al. Intraoperative detection of intimal lipid in the radial artery predicts degree of postoperative spasm. Atherosclerosis 2009; 205: 466–471.

27.

Xiong

, et al. Impact of diabetes mellitus and hemoglobin A1c level on outcomes among Chinese patients with acute coronary syndrome. Clin Cardiol 2020; 43: 723–731.

28.

Cosentino

Grant

Aboyans

, et al. ESC Guidelines on diabetes, pre-diabetes, and cardiovascular diseases developed in collaboration with the EASD. Eur Heart J 2019; 41: 255–323.

29.

Kassimis

Bourantas

Tushar

, et al. Percutaneous coronary intervention vs. cardiac surgery in diabetic patients. Where are we now and where should we be going?. Hellenic J Cardiol 2017; 58: 178–189.

30.

Reis

Allen

Bancks

, et al. Duration of diabetes and prediabetes during adulthood and subclinical atherosclerosis and cardiac dysfunction in middle age: The CARDIA Study. Diabetes Care 2018; 41: 731–738.

31.

Sucato

Novo

Evola

, et al. Coronary microvascular dysfunction in patients with diabetes, hypertension and metabolic syndrome. Int J Cardiol 2015; 186: 96–97.

32.

Herrmann

. Coronary vasa vasorum neovascularization precedes epicardial endothelial dysfunction in experimental hypercholesterolemia. Cardiovascular Res 2001; 51: 762–766.

33.

Kogo

Hiro

Kitano

, et al. Macrophage accumulation within coronary arterial wall in diabetic patients with acute coronary syndrome: a study with in-vivo intravascular imaging modalities. Cardiovascular Diabetology 2020; 19: 135.

34.

Shah

Dolan

Kimball

, et al. Influence of duration of diabetes, glycemic control, and traditional cardiovascular risk factors on early atherosclerotic vascular changes in adolescents and young adults with type 2 diabetes mellitus. J Clin Endocrinol Metab 2009; 94: 3740–3745.

35.

Rossello

Raposeiras-Roubin

Oliva

, et al. Glycated hemoglobin and subclinical atherosclerosis in people without diabetes. J Am Coll Cardiol 2021; 77: 2777–2791.

36.

Zhang

, et al. The independent and add-on values of radial intima thickness measured by ultrasound biomicroscopy for diagnosis of coronary artery disease. Eur Heart J Cardiovasc Imaging 2019; 20: 889–896.

37.

Myredal

Osika

Li Ming

, et al. Increased intima thickness of the radial artery in patients with coronary heart disease. Vasc Med 2010; 15: 33–37.

38.

Koganti

Kotecha

Rakhit

. Choice of intracoronary imaging: When to use intravascular ultrasound or optical coherence tomography. Interv Cardiol 2016; 11: 11–16.

39.

Kurihara

Thondapu

Kim

, et al. Comparison of vascular response to statin therapy in patients with versus without diabetes mellitus. Am J Cardiol 2019; 123: 1559–1564.

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