Blood rheology, fasting serum concentrations of remnant-like lipoprotein particle cholesterol (RLP-C) and concentrations of other lipids were compared in 23 hypercholesterolaemic and 69 normocholesterolaemic subjects, and the relationship between red blood cell (RBC) deformability and RLP-C concentrations were studied in a different set of six hypercholesterolaemic and six normocholesterolaemic subjects. Passage time of whole blood and concentrations of total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and RLP-C were significantly higher in hypercholesterolaemic than in normocholesterolaemic subjects. Passage time of whole blood correlated positively with TC, TG, LDL-C and RLP-C and negatively with high-density lipoprotein cholesterol. Furthermore, the passage time of 10% haematocrit-adjusted RBCs in phosphate-buffered saline, which reflects RBC deformability, correlated positively with the passage time of whole blood and RLP-C. Thus, hypercholesterolaemic subjects had impaired blood rheology and elevated RLP-C concentrations, which may be associated with the pathophysiology of atherosclerosis in hypercholesterolaemic subjects. Impaired RBC deformability may contribute to impaired blood rheology associated with elevated RLP-C in hypercholesterolaemic subjects.
MartinMJHulleySBBrownerWS: Serum cholesterol, blood pressure, and mortality: Implications from a cohort of 361,662 men. Lancet1982; ii: 933–936.
2.
PhilipsNRWatersDHavelRJ: Plasma lipoproteins and progression of coronary artery disease evaluated by angiography and clinical events. Circulation1993; 88: 2762–2770.
3.
ZilversmitDB: Atherogenic nature of triglycerides, postprandial lipemia, and triglyceride-rich remnant lipoproteins. Clin Chem1995; 41: 153–158.
4.
NakajimaKSaitoTTamuraA: Cholesterol in remnant like lipoproteins in human serum using monoclonal anti-apo B-100 and anti-apo A-I immunoaffinity mixed gels. Clin Chim Acta1993; 23: 53–71.
5.
McNamaraJRShahPKNakajimaK: Remnant lipoprotein cholesterol and triglyceride reference ranges from the Framingham Heart Study. Clin Chem1998; 44: 1224–1232.
6.
ErnstEReschKL: Fibrinogen as a cardiovascular risk factor: A meta-analysis and review of the literature. Ann Intern Med1993; 118: 956–963.
7.
DaneshJCollinsRApplebyP: Association of fibrinogen, C-reactive protein, albumin, or leukocyte count with coronary heart disease: Meta-analysis of prospective studies. JAMA1998; 279: 1477–1482.
8.
YarnellJWBakerIASweetnamPM: Fibrinogen, viscosity, and white blood cell count are major risk factors for ischemic heart disease. The Caerphilly and Speedwell collaborative heart disease studies. Circulation1991; 83: 836–844.
9.
KoenigWSundMFilipiakB: Plasma viscosity and the risk of coronary heart disease. Results from the MONICA-Augsburg Cohort Study, 1984 to 1992. Arterioscler Thromb Vasc Biol1998; 18: 768–772.
10.
LoweGDLeeAJRumleyA: Blood viscosity and risk of cardiovascular events: The Edinburgh Artery Study. Br J Haematol1997; 96: 168–173.
11.
LeeAJMowbrayPILoweGD: Blood viscosity and elevated carotid intima-media thickness in men and women. The Edinburgh Artery Study. Circulation1998; 97: 1467–1473.
12.
KoenigWSundMErnstE: Association between rheology and components of lipoproteins in human blood: Results from the MONICA Project Augsburg. Circulation1992; 85: 2197–2204.
13.
LoweGDWoodDADouglasJT: Relationships of plasma viscosity, coagulation and fibrinolysis to coronary risk factors and angina. Thromb Haemost1991; 65: 339–343.
14.
SuryaIIAkkermanJW: The influence of lipoproteins on blood platelets. Am Heart J1993; 125: 272–275.
15.
KuriharaTDeguchiSKatoJ: Impaired blood rheology by remnant-like lipoprotein particles: Studies in patients with fatty liver disease. Clin Hemorheol Microcirc2001; 24: 217–225.
16.
KikuchiYSatoKOhkiH: Optically accessible microchannels formed in a single-crystal silicon substrate for studies of blood rheology. Microvasc Res1992; 44: 226–240.
17.
SekiKSuminoHNaraM: Relationships between blood rheology and age, body mass index, blood cell count, fibrinogen, and lipids in healthy subjects. Clin Hemorheol Microcirc2006; 34: 401–410.
18.
SuminoHNaraMSekiK: Effect of antihypertensive therapy on blood rheology in patients with essential hypertension. J Int Med Res2005; 33: 170–177.
19.
SuminoHIchikawaSTakahashiT: Conjugated estrogen plus medroxyprogesterone does not impair blood rheological properties in hypertensive postmenopausal women. Maturitas2005; 53: 306–314.
20.
MuranakaYKunimotoFTakitaJ: Impaired blood rheology in critically ill patients in intensive care unit. J Int Med Res2006; 34: 419–427.
21.
TsukadaKSekizukaEOshioC: Direct measurement of erythrocyte deformability in diabetes mellitus with a transparent microchannel capillary model and high-speed video camera system. Microvasc Res2001; 61: 231–239.
22.
Japanese Atherosclerosis Society: Investigating Committee of Guidelines for Diagnosis and Treatment of Hyperlipidemias. J Jpn Atheroscler Soc1997; 25: 1–34.
23.
LeeCYJKimKCParkHW: Rheological properties of erythrocytes from male hypercholesterolemia. Microvasc Res2004; 67: 133–138.
24.
RosensonRSLoweGD: Effects of lipids and lipoproteins on thrombosis and rheology. Atherosclerosis1998; 140: 271–280.
25.
KanakarajiPSinghM: Influence of hypercholesterolemia on morphological and rheological characteristics of erythrocytes. Atherosclerosis1989; 76: 209–218.
26.
ChabanelAFlammMSungKL: Influence of cholesterol content on red cell membrane viscoelasticity and fluidity. Biophys J1983; 44: 171–176.
27.
SeplowitzAHChienSSmithEF: Effects of lipoproteins on plasma viscosity. Atherosclerosis1981; 38: 88–95.
28.
LeonhardtHArntzHRKlemensUH: Studies of plasma viscosity in primary hyperlipoproteinemia. Atherosclerosis1977; 28: 29–40.
29.
EpandRMStaffordALeonB: HDL and apolipoprotein A-1 protect erythrocytes against the generation of procoagulant activity. Arterioscler Thromb1994; 14: 1775–1783.
30.
CichaISuzukiYTateishiN: Effects of dietary triglycerides on rheological properties of human red blood cells. Clin Hemorheol Microcirc2004; 30: 301–305 [abstract].
31.
RosensonRSShottSLuL: Hypertriglyceridemia and other factors associated with plasma viscosity. Am J Med2001; 110: 488–492.
32.
ContrerasTVayáAPalancaS: Influence of plasmatic lipids on the hemorheological profile in healthy adults. Clin Hemorheol Microcirc2004; 30: 423–425.
33.
Dane-StewartCAWattsGFMamoCJL: Elevated apolipoprotein B-48 and remnant-like particle-cholesterol in heterozygous familial hypercholesterolemia. Eur J Clin Invest2001; 31: 113–117.
34.
SaniabadiARUmemuraKShimoyamaM: Aggregation of human blood platelets by remnant-like lipoprotein particles of plasma chylomicrons and very low density lipoproteins. Thromb Haemost1997; 77: 996–1001.
35.
SaniabadiARUmemuraKSuzukiY: Adenosine 5î-diphosphate as a factor in platelet aggregation induced by human plasma remnant lipoproteins. Life Sci1998; 63: 1065–1074.
