Cellular Distribution of Tumour Suppressor Protein p53 and High-Risk Human Papillomavirus (HPV)-18 E6 Fusion Protein in Wild-Type p53 Cell Lines

Abstract

Human papillomaviruses (HPVs) are very important pathogens that can be classified as high- and low-risk types based on the lesions they cause. Mucosal high-risk HPV E6 can target and degrade the tumour suppressor p53, hence it is recognized as the major cause of cervical cancer, however, due to a lack of reliable anti-E6 antibodies, the distribution of high-risk HPV E6 protein remains elusive. The present study, therefore, used a mammalian green fluorescent protein (GFP) expression system to express GFP-18 E6 fusion proteins in wild-type p53 cells, SMMC-7721 and HCT116, in order to trace the location and expression of HPV E6 and p53 protein. Following transfection, expression of GFP-18 E6 was found to be located in the nucleus, and endogenous wild-type p53 was also located there with GFP-18 E6.