This study evaluated the relationship between the activity of the inflammatory indicator adenosine deaminase (ADA) in placental tissue and maternal and fetal (umbilical cord) plasma and the severity of pre-eclampsia. Maternal and umbilical vein whole blood and placental tissue samples were collected from women with normal pregnancies (n = 33) and patients with mild (n = 12) or severe (n = 17) pre-eclampsia. ADA activity was measured spectrophotometrically. Significantly increased ADA activity was detected in maternal and fetal plasma, and placental tissue in patients with mild and severe pre-eclampsia compared with normal pregnancies; there were no significant differences between the mild and severe cases. The presence of increased ADA activity in pre-eclampsia is consistent with activation of the inflammatory system in this condition. The increased ADA activity was related to the presence of the disease but not the severity of clinical symptoms. Neonatal outcome did not significantly correlate with observed ADA activity.
SibaiBM: Preeclampsia: An inflammatory syndrome?. Am J Obstet Gynecol2004; 191: 1061–1062.
2.
LalaPKChakrabortyC: Factors regulating trophoblast migration and invasiveness: Possible derangements contributing to pre-eclampsia and fetal injury. Placenta2003; 24: 575–587.
3.
RedmanCWGSargentIL: Pre-eclampsia, the placenta and the maternal systemic inflammatory response–a review. Placenta2003; 24 (Suppl A): S21–S27.
4.
AbrahamsVMKimYMStraszewskiSLRomeroRMorG: Macrophages and apoptotic cell clearance during pregnancy. Am J Reprod Immunol2004; 51: 275–282.
5.
SargentILGermainSJSacksGPKumarSRedmanCWG: Trophoblast deportation and the maternal inflammatory response in pre-eclampsia. J Reprod Immunol2003; 59: 153–160.
6.
FaasMMSchuilingGABallerJFWVisscherCABakkerWW: A new animal model for human preeclampsia: Ultra-low-dose endotoxin infusion in pregnant rats. Am J Obstet Gynecol1994; 171: 158–164.
7.
AdamsAHarknessRA: Adenosine deaminase activity in thymus and human tissues. Clin Exp Immunol1976; 26: 647–649.
CristalliGCostanziSLambertucciCLupidiGVittoriSVolpiniR: Adenosine deaminase: Functional implications and different classes of inhibitors. Med Res Rev2001; 21: 105–128.
10.
GakisC: Adenosine deaminase (ADA) isoenzymes ADA1 and ADA2: Diagnostic and biological role. Eur Respir J1996; 9: 632–633.
11.
ConlonBALawWR: Macrophages are a source of extracellular adenosine deaminase-2 during inflammatory responses. Clin Exp Immunol2004; 138: 14–20.
12.
YoneyamaYSawaRSuzukiSOtsuboYMiuraAKuwabaraY: Serum adenosine deaminase activity in women with pre-eclampsia. Gynecol Obstet Invest2002; 54: 164–167.
13.
YoneyamaYSawaRSuzukiSDoiDYoneyamaKOtsuboY: Relationship between plasma malondialdehyde levels and adenosine deaminase activities in pre-eclampsia. Clin Chim Acta2002; 322: 169–173.
14.
KatoHYoneyamaYArakiT: Fetal plasma lipid peroxide levels in pregnancies complicated by preeclampsia. Gynecol Obstet Invest1997; 43: 158–161.
15.
DavidgeSTSignorellaAPLykinsDLGilmourCHRobertsJM: Evidence of endothelial activation and endothelial activators in cord blood of infants of pre-eclamptic women. Am J Obstet Gynecol1996; 175: 1301–1306.
16.
FriedmanSASchiffEEmeisJJDekkerGAKaoLSibaiBM: Fetal plasma levels of cellular fibronectin as a measure of fetal endothelial involvement in preeclampsia. Obstet Gynecol1997; 89: 46–48.
17.
ACOG Committee on Practice Bulletins -Obstetrics: Diagnosis and management of preeclampsia and eclampsia. ACOG practice bulletin no. 33. Obstet Gynecol2002; 99: 159–167.
18.
LowryOHRosebroughNJFarrALRandallRJ: Protein measurement with the Folin phenol reagent. J Biol Chem1951; 193: 265–275.
KarabulutABKafkasliABurakFGozukaraEM: Maternal and fetal plasma adenosine deaminase, xanthine oxidase and malondialdehyde levels in pre-eclampsia. Cell Biochem Funct2005; 23: 279–283.
21.
JaquetiJMartinez-HernandezDHernandez-GarciaRNavarro-GallarFArenas-BarberoJ: Adenosine deaminase in pregnancy serum. Clin Chem1990; 36:2144.
22.
SuzukiSYoneyamaYSawaRArakiT: Relation between maternal serum adenosine deaminase and plasma adenosine levels in twin pregnancies. Clin Biochem2002; 35: 417–419.
23.
YoneyamaYSuzukiSSawaROtsuboYPowerGGArakiT: Plasma adenosine levels increase in women with normal pregnancies. Am J Obstet Gynecol2000; 182: 1200–1203.
24.
YoneyamaYSawaRSuzukiSMiuraAKobayashiHDoiD: Relation between adenosine deaminase activities and cytokine-producing T cells in women with preeclampsia. Clin Biochem2002; 35: 303–306.
25.
WegmannTGLinHGuilbertLMosmannTR: Bidirectional cytokine interactions in the maternal-fetal relationship: Is successful pregnancy a TH2 phenomenon?. Immunol Today1993; 14: 353–356.
BenyoDFSmarasonARedmanCWSimsCConradKP: Expression of inflammatory cytokines in placentas from women with pre-eclampsia. J Clin Endocrinol Metab2001; 86: 2505–2512.
28.
MervielPCarbillonLChallierJCRabreauMBeaufilsMUzanS: Pathophysiology of preeclampsia: Links with implantation disorders. Eur J Obstet Gynecol Reprod Biol2004; 115: 134–147.
29.
SaitoSSakaiM: Th1/Th2 balance in preeclampsia. J Reprod Immunol2003; 59: 161–173.
30.
RobertsJMLainKY: Recent insights into the pathogenesis of pre-eclampsia. Placenta2002; 23: 359–372.
