Background: Myelin oligodendrocyte glycoprotein (MOG) may be implicated in the immunopathogenesis of multiple sclerosis (MS) inducing demyelination in the animal model of MS. In adults reported anti-MOG antibody frequencies have been variable across a number of studies and can also be detected in controls.
Objective: To measure antibodies against MOG in paediatric patients with demyelinating disorders of the central nervous system and in controls.
Methods: Serum antibodies against MOG and myelin basic protein were measured by ELISA, flow cytometry (FACS) and in the liquid phase in 11 children with acute disseminated encephalomyelitis (ADEM), 22 children with MS, seven children with acute viral encephalitis and 13 healthy controls. The serostatus of Epstein–Barr virus (EBV) infections were assessed.
Results: Anti-MOG antibodies, measured either by ELISA or FACS were exclusively detected in children with demyelination. In ADEM these antibodies were highly reactive. Anti-MBP reactivity was detectable equally in all groups. The presence of either autoantibodies did not associate with EBV serostatus, age, gender or disease course.
Conclusions: This study independently corroborates recently published results of seroprevalence and specificity of the assay. Due to their low sensitivity anti-MOG antibodies will not serve as disease-specific biomarkers, but could help to support the diagnosis of ADEM in difficult cases.
SospedraMMartinR. Immunology of multiple sclerosis. Annu Rev Immunol2005; 23: 683–747.
2.
von BüdingenHCTanumaNVillosladaPOualletJCHauserSLGenainCP. Immune responses against the myelin/oligodendrocyte glycoprotein in experimental autoimmune demyelination. J Clin Immunol2001; 21: 155–170.
3.
ReindlMKhalilMBergerT. Antibodies as biological markers for pathophysiological processes in MS. J Neuroimmunol2006; 180: 50–62.
4.
O’ConnorKCMcLaughlinKADe JagerPLChitnisTBettelliEXuC. Self-antigen tetramers discriminate between myelin autoantibodies to native or denatured protein. Nat Med2007; 13: 211–217.
5.
McLaughlinKAChitnisTNewcombeJFranzBKennedyJMcArdelS. Age-dependent B cell autoimmunity to a myelin surface antigen in pediatric multiple sclerosis. J Immunol2009; 183: 4067–4076.
6.
SelterRCBrilotFGrummelVKrausVCepokSDaleRC. Antibody responses to EBV and native MOG in pediatric inflammatory demyelinating CNS diseases. Neurology2010; 74: 1711–1715.
7.
BrilotFDaleRCSelterRCGrummelVKalluriSRAslamM. Antibodies to native myelin oligodendrocyte glycoprotein in children with inflammatory demyelinating central nervous system disease. Ann Neurol2009; 66: 833–842.
8.
AscherioAMungerKL. Environmental risk factors for multiple sclerosis. Part I: The role of infection. Ann Neurol2007; 61: 288–299.
9.
FarrellRAAntonyDWallGRClarkDAFisnikuLSwantonJ. Humoral immune response to EBV in multiple sclerosis is associated with disease activity on MRI. Neurology2009; 73: 32–38.
10.
WucherpfennigKWStromingerJL. Molecular mimicry in T cell-mediated autoimmunity: viral peptides activate human T cell clones specific for myelin basic protein. Cell1995; 80: 695–705.
11.
LunemannJDJelcicIRobertsSLutterottiATackenbergBMartinR. EBNA1-specific T cells from patients with multiple sclerosis cross react with myelin antigens and co-produce IFN-gamma and IL-2. J Exp Med2008; 205: 1763–1773.
12.
MengeTvon BüdingenHCLalivePHGenainCP. Relevant antibody subsets against MOG recognize conformational epitopes exclusively exposed in solid-phase ELISA. Eur J Immunol2007; 37: 3229–3239.
13.
LalivePHMengeTDelarasseCDellaGBPham-DinhDVillosladaP. Antibodies to native myelin oligodendrocyte glycoprotein are serologic markers of early inflammation in multiple sclerosis. Proc Natl Acad Sci U S A2006; 103: 2280–2285.
WangHMungerKLReindlMO'ReillyEJLevinLIBergerT. Myelin oligodendrocyte glycoprotein antibodies and multiple sclerosis in healthy young adults. Neurology2008; 71: 1142–1146.
16.
MengeTHemmerBNesslerSWiendlHNeuhausOHartungHP. Acute disseminated encephalomyelitis: an update. Arch Neurol2005; 62: 1673–1680.
17.
GuggenmosJSchubartASOggSAnderssonMOlssonTMatherIH. Antibody cross-reactivity between myelin oligodendrocyte glycoprotein and the milk protein butyrophilin in multiple sclerosis. J Immunol2004; 172: 661–668.
18.
HughesLESmithPABonellSNattRSWilsonCRashidT. Cross-reactivity between related sequences found in Acinetobacter sp., Pseudomonas aeruginosa, myelin basic protein and myelin oligodendrocyte glycoprotein in multiple sclerosis. J Neuroimmunol2003; 144: 105–115.
