A Transcreener™ kinase fluorescence polarization (FP) assay has been developed for the serine/threonine kinase protein kinase A (PKA). The PKA Transcreener™ kinase assay is an homogenous, competitive antibody-based FP assay that uses Far Red Alexa Fluor 633-labeled adenosine 5′ disphosphate (ADP) tracer and mouse monoclonal anti-ADP antibody. The Transcreener™ PKA assay was validated with both known PKA inhibitors and library compounds. The Transcreener™ PKA assay is resistant to low-wavelength (or common) fluorescent interference from small-molecule library compounds and generates IC50 results comparable with current radioactive filter-binding assay. (Journal of Biomolecular Screening 2007:578-584)
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