The effects of different cooking techniques on the lipids of pork meat were evaluated. The assayed cooking techniques were deep-frying (DF), shallow-frying (SF), roasting (RO) and boiling (BO). Modi fication of the roasting meat due to reheating in a microwave oven after 24 h at 4 °C (MW), was also studied. In DF and SF significant increases were found in the lipid content (13.7 and 16.7 g/100 g of dry basis, respectively) and in the nun-polar lipids (11.2 and 14.2 g/100 g of dry basis, respectively) due to the absorption of the oil used in these cooking treatments. The main variations in the fatty acid composition of phospholipids and non-polar lipids were observed in the polyunsaturated fatty acid fraction. There was an increase in the content of free fatty acids (SFA, MUFA, PUFA) after MW, BO and RO. By contrast, free fatty acid content in DF and SF was similar to the content of initial raw meat. MW showed the greatest degradation of the phospholipid fraction, with a significant decline in phosphatidyl ethanolamine. All cooking treatments produced an increase in lipid oxidation, this be ing higher in RO and MW treatments.
Allen C.E. and Foegeding E.A. (1981). Some lipid characteristics and interactions in muscle foods. A review. Food Technology35: 253-257.
2.
Barlett G.R. (1959). Phosphorous assay in column chromatography. Journal of Biological Chemistry234 : 466-468.
3.
Berry J.F., Cevallos W.H. and Wade R.R. (1965). Lipid class and fatty acid composition of intact peripheral nerve and during wallerian degeneration. Journal of American Oil Chemistry Society42 : 492-495.
4.
Candela M., Astiasaran I. and Bello J. (1996). Effect of frying on the fatty acid profile of some meat dishes. Journal of Food Composition and Analysis9: 277-282.
5.
Canell L.E., Savell J.W., Smith S.B., Cross H.R. and St. John L.C. (1989). Fatty acid composition and caloric value of ground beef containing low levels of fat. Journal of Food Science54: 1163-1168.
6.
Fogerty A.C., Whitfield F.B., Svoronos D. and Ford G.L. (1989). Effect of heat on the fatty acids and aldehydes of veal meat pliospholipids. International Journal of Food Science and Technology24 : 529-534.
7.
Folch J., Lees M. and Sloane-Stanley G.H.S. (1957). A simple method of isolation and purification of total lipids from animal tissues. Journal of Biological Chemistry226: 497-509.
8.
Gandemer G. (1990). Les phospholipides du muscle: composition et altération au cours des traitements technologiques. Revue Française des Corps Gras37: 75-81.
9.
Gandemer G. (1992). Les lipides de la viande: vers une estimation precise de leurs apports nutritionnels dans l'alimentation de l'homme. Les Cahiers de L'ENSBANA8 : 25-48.
10.
Gandemer G. and Meynier A. (1995). The importance of phospholipids in the development of flavour and off-flavour in meat products. Uppsala, Sweden: ECCEAMST.
11.
Guilland J.C., Kokkidis M.J. and Rivera M.A. (1992). Effect du stockage au froid et du mode de cuisson sur la composition de la viande bovine. Cahier des Nutrition et Diétetique4: 251-256.
12.
Hamm R. and Deatherage F.E. (1960). Changes in hydration, solubility and charges of muscle proteins during heating of meat. Food Research25: 587-610.
13.
Hernández P., Navarro J.L. and Toldrá F. (1998). Lipid composition and lipolytic enzyme activities in porcine skeletal muscle with different oxidative pattern. Meat Science49: 1-10.
14.
Igene J.O. and Pearson A.M. (1979). Role of phospholipids and triglycerides in warmed-over flavor development in meat model systems. Journal of Food Science44: 1285-1290.
15.
Igene J.O., Pearson A.M. and Gray J.I. (1981). Effects of length of frozen storage, cooking and holding temperatures upon component phospholipids and the fatty acid composition of meat triglycerides and phospholipids. Food Chemistry7: 289-303.
16.
Juaneda P. and Rocquelin G. (1985). Rapid and convenient separation of phospholipids and non phosphorus lipids from rat heart using silica cartridges. Lipids20: 40-41.
17.
Kanner J. (1994). Oxidative processes in meat and meat products : quality implications. Meat Science36: 169-189.
18.
Keller J.D. and Kinsella J.E. (1973). Phospholipids changes and lipid oxidation during cooking and frozen storage of raw ground beef. Journal of Food Science38: 1200-1204.
19.
Keseva Rao V., Kowale B.N., Babu N.P. and Bisht G.S. (1996). Effect of cooking and storage on lipid oxidation and development of cholesterol oxidation products in water buffalo meat. Meat Science43: 179-185.
20.
Kowale B.N., Keseva Rao V., Babu N.P., Sharma N. and Bisht G.S. (1996). Lipid oxidation and cholesterol oxidation in mutton during cooking and storage. Meat Science43: 195-202.
21.
Leseigneur-Meynier A. and Gandemer G. (1991). Lipid composition of pork muscle as related to metabolic types of fibres. Meat Science29: 229-241.
22.
Melton S.L. (1983). Methodology for following lipid oxidation in muscle foods. Food Technology37: 105-111.
23.
Morrison W.R. and Smith L.M. (1964). Preparation of fatty acids methyl esters and dimethyl acetals from lipids with boron fluoride-methanol. Journal of Lipid Research5: 600-608.
24.
Motilva M.J., Toldrá F. and Flores J. (1992). Assay of lipase and esterase activities in fresh pork meat and dry-cured ham. Zeitshrift für Lebensmittel Untersuchung und Forschung195 : 446-450.
25.
Needs E.C., Ford G.D., Owen A.J. and Tuckley B. (1983). A method for the quantitative determination of individual free fatty acids in milk by ion exchange resin adsorption and gas-liquid chromatography. Journal of Dairy Research50: 321-329.
26.
Newburg D.S. and Concon J.M. (1980). Malonaldehyde concentrations in food are affected by cooking conditions. Journal of Food Science45: 1681-1687.
27.
Ngah E., Alasnier C. and Gandemer G. (1994). Molecular species composition of phosphatidylcholine and phosphatidylethanolamine in skeletal muscle of farm animals. In: 40 th International Congress of Meat Science and Technology . The Hague, The Netherlands. s-v. 15.
28.
Pearson A.M., Gray J.I., Wolzak A.M. and Horenstein N.A. (1983). Safety implications of oxidized lipids in muscle foods. Food Technology37: 121-129.
29.
Pérez-Camino M.C., Márquez-Ruiz G., Ruiz-Méndez M.V. and Dobarganes M.C. (1991). Lipid changes during frying of frozen prefried foods. Journal of Food Science56: 1644-1650.
30.
Pikul J., Leszczynski D.E., Niewiarowich A. and Kummerow F.A. (1984). Lipid oxidation in chicken breast and leg meat after sequential treatments of frozen storage, cooking, refrigerated storage and reheating. Journal of Food Technology19: 575-584.
31.
Raharjo S., Sofos J.N. and Schmidt G.R. (1992). Improved speed, specificity and limit of determination of an aqueous acid extraction thiobarbituric acid-C18 method for measuring lipid oxidation. Journal of Agricultural and Food Chemistry40: 2182-2185.
32.
Rodriguez-Estrada M.T., Penazzi G., Caboni M.F., Bertacco G. and Lercker G. (1997). Effect of different cooking methods on some lipid and protein components of hamburgers. Meat Science45: 365-375.
33.
Salih A.M., Price.J.F., Smith D.M. and Dawson L.E. (1989). Lipid degradation in turkey breast meat during cooking and storage. Poultry Science68: 754-761.
34.
Sas (1990). SAS/STAT User's Guide. Version 6, SAS Institute Inc., Cary, NC, USA.
35.
Satyanarayan V.T. and Honikel K.O. (1992). Effect of different cooking methods on warmed-over flavour development in pork. Zeitschrift für Lebensmittel Untersuchung und Forschung194 : 422-425.
36.
Shantha N.C. and Decker E.A. (1994). Rapid, sensitive, iron-based spectrophotometric methods for determination of peroxide values of food lipids. Journal of AOAC International77 : 421-424.
37.
Stapelfeldt H., Bjorn H., Skovgaard I.M., Skibsted L.H. and Bertelsen G. (1992). Warmed-over flavour in cooked sliced beef. Zeitschrift für Lebensmittel Untersuchung und Forschung195: 203-208.
38.
Tims M.J. and Watts B.M. (1958). Protection of cooked meats with phosphates. Food Technology12 : 240-243.
39.
Wilson F.B., Pearson A.M. and Shorland F.B. (1976). Effect of total lipids and phospholipids on warmed-over-flavor in red and white muscle from several species as measured by thiobarbituric acid analysis. Journal of Agricultural and Food Chemistry24: 7-11.
