Effect of maceration time and the addition of enzymes on the amino acid composition of musts and wines and its influence on wine aroma Influencia del tiempo de maceración y de la adición de enzimas sobre la composición de los aminoácidos de mostos y vinos y su relación con el aroma

Abstract

The purpose of this work is to study the effect of maceration time (with and without pectolitic en zyme addition) on the concentrations of 18 amino acids found in must and wines of the Macabeo grape variety. The presence of enzymes had a significant effect on the relative composition of the amino acids. The maceration process, with or without enzymes, led to a decrease in amino acid con tent after 2 h of maceration. After 2 h the solid parts of the grape released amino acids into the must, with the maximum concentration reached after 6 h. A certain degree of correlation given by principal component analysis was observed between the concentration of valine, isoleucine and histidine present in the musts and the aromatic compounds in the wines related to the metabolism of the fatty acids (hexanoic acid, octanoic acid, ethyl octanoate, hexanoate and decanoate). For the wines, a high corre lation was found between valine and isobutanol and a lower value for valine with isoamyl alcohol and β-phenyl ethanol.

Keywords

amino acids must wine maceration enzymes aroma

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References

Arnold R.A. and Noble A.C. (1979). Effect of pomace contact on the flavor of Chardonnay wine. American Journal of Enology nnd Viticulture 30 (3): 179-181.

Barillere J.M. , Samson A. , Bayonove C. and Bouvier J.C. (1990). Analyses multidimensionnelles sur des caractéristiques chimiques et organoleptiques de vins blancs obtenus par macération pelliculaire . Revue Française d'Oenologie 123: 14-20.

Castino M. , Bosso A. and Marescalco J. (1990). Elaborazione di vini bianchi con macerazione a freddo e in presenza di enzimi pectolitici. Vini d'Italia 5: 7-20.

Defranoux C. and Joseph P. (1992). Une décennie consacrée à la connaissance du potentiel aromatique du Chardonnay. Revue des Oenologues 65: 27-29.

Dubourdieu D. , Ollivier C.H. and Boidron J.N. (1986). Incidence des operations prefermentaires sur la composition chimique et les qualites organoleptiques des vins blancs secs . Connaissance de la Vigne et du Vin 20 (1): 53-76.

Ferreira V. , Rapp A. , Cacho J. , Hastrich H. and Yavas I. (1993). Fast and quantitative determination of wine flavor compounds using microextraction with Freon 113. Journal of Agricultural and Food Chemistry 41: 1413-1420.

Feuillat M. , Brillant G. and Rochard J. (1980). Mise en evidence d'une production de proteases exocellulaires par les levures au cours de la fermentation alcoolique du moût de raisin. Connaissance de la Vigne et du Vin 14: 37-52.

Gracia P. (1996). Acidez volatil y paradas de fermentacion. Estudios de factores que las originan y de sus posibles soluciones. Thesis Doctoral, Universidad de Zaragoza, Spain.

Guitart A. , Hernandez-Orte P. and Cacho J. (1996). Optimization of amino acid derivatization by phenylisothiocyanate and separation by HPLC. Quimica Analítica 15: 217-223.

10.

Guitart A. , Hernandez-Orte P. and Cacho J. (1997). Effects of maceration on the amino acid content of Chardonnay musts and wines. Vitis 36(1): 43-47.

11.

Haushoffer H. (1978). Conservation des vins blancs aromatiques. Annales du Technologie Agricole 27(1): 221-230.

12.

Hernandez-Orte P. , Guitart A. and Cacho J. (1997). Amino acid determination in musts and wines by HPLC after derivatization with phenylisothiocyanate. American Journal of Enology and Viticulture 48 (2): 229-235.

13.

Rapp A. and Versini G. (1991). Influence of nitrogen compounds in grapes on aroma compounds of wines. In: Rantz J. (ed.), Proceedings of the International Symposium on Nitrogen in Grapes and Wines. Davis, CA: American Society for Enology and Viticulture. pp. 156-164.

14.

Rohlf F.J. (1991). NTSYS—PC, Numerical Taxonomy And Multi-variate Analysis System Version 1.70. Setauket, New York: Exeter Publishing Ltd.

15.

Singleton V.L. , Sieberhagen H.A.M. , De Wet, P. and Van Wyk C.J. (1975). Composition and sensory qualities of wines prepared for white grapes by fermentation with and without grape solids. American Journal of Enology and Viticulture 26(2): 62-69.

16.

Usseglio-Tomasset L. and Bosia P.D. (1990). Evolution des acides amines et des oligopeptides du moût au vin. Bulletin de L' O. I. V. 707-708: 21-46.