Enzymatic browning and its inhibition in new apple cultivars slices using 4-hexylresorcinol in combination with ascorbic acid / Pardeamiento enzimático y su inhibición en rodajas de manzanas de nuevas variedades utilizando 4-hexilresorcinol en combinación con ácido ascórbico

Abstract

Enzymatic browning in minimally processed apples of new cultivars (i.e. Braeburn, Criterion, Fuji, Gala, Jonagold) was investigated at 2.5, 12 and 20 °C, and compared with traditional cultivars (Golden Delicious and McIntosh). Browning inhibition by ascorbic acid (AA) and a mixture of ascorbic acid and 4-hexylresorcinol (AH) was also studied. Among the new cultivars, Jonagold had the slowest browning rate and Braeburn had the fastest rate. Of the two traditional cultivars, McIntosh had the faster browning rate. The temperature sensitivity of the browning varied among cultivars, where Q ₁₀ ranged from 1.3 to 4.4 (calculated from colour parameter a*), and the acti vation energy (E _a) ranged from 8.0 to 18.3 kcal/mol. The browning rate dependence on temper ature was as follows: Fuji < McIntosh < Criterion < Braeburn and Gala < Golden Delicious < Jonagold. Browning was inhibited significantly using AH and AA. The AH treatment resulted in a better inhibition of enzymatic browning for most of the cultivars.

Keywords

enzymatic browning apple cultivars 4-hexylresorcinol ascorbic acid

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References

Burda S. , Oleszek W. and Lee C.Y. (1990). Phenolic compounds and their changes in apples during maturation and cold storage. Journal of Agricultural and Food Chemistry 38: 945-948.

Coseteng M.Y. and Lee C.Y. (1987). Changes in apple polyphenoloxidase and phenol concentration in relation to degree of browning. Journal of Food Science 42: 985-989.

Fleming T. (1991). New varieties, a look at Fuji. Annual Report of the Michigan State Horticulture Society 121: 184-185.

Food and Drug Administration (1986). Sulfiting agents; Revocation of GRAS status for use on fruits and vegetables intended to be served or sold raw to consumers. Federal Regional 51: 25021-25026.

Food and Drug Administration (1990). Sulfiting agents; Revocation of GRAS status for use on fresh potatoes served or sold unpackaged or unlabeled to consumers. Federal Regional 55: 9826-9833.

Golan-Goldhirsh A. , Whitaker J.R. and Kahn V. (1984). Relation between structure of potyphenotoxidase and prevention of browning. Advances in Experimental Medicine and Biology 177: 437-456.

Hape G.W. (1961). Sulfite inhibition of polyphenoloxidase. Food Technology 15: 548.

Kim D.M. , Smith N.L. and Lee C.Y. (1993). Quality of minimally processed apple slices from selected cultivars. Journal of Food Science 58: 1115-1117, 1175.

Luo Y. and Barbosa-Cánovas G.V. (1995) Inhibition of apple-slices browning by 4-hexylresorcinol . In Lee C.Y. and Whitaker J.R. (eds), ACS Symposium Series 600. Washington, DC: American Chemical Society. pp. 240-250.

10.

McKenzie D.W. (1984). Trends in apple varieties in New Zealand. Proceedings of the Washington State Horticulture Society 80: 161-166.

11.

McEviley A.J. , Iyengar R. and Gross A. (1991). Compositions and methods for inhibiting browning in food using resorcinol derivatives. US Patent 5,054,438.

12.

McEviley A.J. , Iyengar R. and Gross, A. (1992). Inhibition of Enzymatic Browning in Foods and Beverages. CRC Critical Reviews in Food Science and Nutrition 32: 253-273.

13.

Miller S.S. (1991). Apple cultivars: current situation and trends around the world. An introduction. Fruit Varieties Journal 45: 75-76.

14.

Monsalve-González A. , Barbosa-Cánovas G.V. , Cavalieri R.P. , McEviley A.J. and lyengar R. (1993). Control of browning during storage of apple slices preserved by combined methods. 4-Hexylresorcinol as an anti-browning agent . Journal of Food Science 58: 797-800, 826.

15.

Nicolas J. , Richard-Forget F.C. , Goupy P.M. , Amiot M.J. and Aubert S.Y. (1994). Enzymatic browning reaction in apple and apple products. CRC Critical Reviews in Food Science and Nutrition 34(2): 109-157.

16.

Oleszek W. , Lee C.Y. and Price KR (1989). Apple phenolics and their contribution to the enzymatic browning reactions. Acta Societatis Botanicorum Poloniae 58: 273.

17.

Ponting J.D. , Jackson R. and Watter G. (1972). Refrigerated apple slices, preservative effects of ascorbic acid, calcium and sulfites. Journal of Food Science 37 434-436.

18.

Sapers G.M. and Douglas F.W. (1987). Measurement of enzymatic browning at cut surfaces and in juice of raw apple and pear fruits. Journal of Food Science 5: 1258-1262, 1285.

19.

Sapers G.M. (1993). Browning of foods: control by sulfites, antioxidants, and other means. Food Technology 47(10): 75-84.

20.

Sas Inc. (1985). SAS User's Guild, Statistic. 5th edn. Cary, NC: SAS Institute, Inc.

21.

Schechter Y. and Proctor J.T.A. (1959) Jonagold: an apple for the 21st century. Fruit Varieties Journal 43(1): 4-6.

22.

Taylor S.L. , Higley N.A. and Bush R.K. (1986). Sulfites in foods, uses, analytical methods, residues, fate, exposure assessment, metabolism, toxicity, and hypersensitivity. Advances in Food Research 30: 1-76.

23.

Vámos-Vigyázo L. (1981). Polyphenoloxidase and peroxidase in fruits and vegetables. CRC Critical Reviews in Food Science and Nutrition 152 (1): 49-127.