Restricted accessResearch articleFirst published online 2025
Phytochemical profiling of sorghum grains ( Sorghum bicolor ) irradiated with gamma rays,low energy electron beam (LEEB) and high energy electron beam (HEEB)
The study evaluated the profile of some phytochemicals in sorghum grains decontaminated using gamma rays, low and high energy electron beams. Samples were purchased from a major sorghum outlet and were packaged appropriately. The packaged grains were exposed to target irradiation doses of 2, 4, 6, 8 and 10 kGy, and the un-irradiated sorghum grains served as a control. The ILU-6 accelerator (250 keV), ELEKTRONIKA 10-10 accelerator (9 MeV) and Gamma Chamber 5000 Co-60 source were used for low electron beam, high electron beam and gamma irradiation, respectively. Reverse-phase high-performance liquid chromatography was used to identify and quantify the flavonoids and phenolic acids in the samples. Antioxidant activity was determined using hydrogen peroxide (H2O2) scavenging assay and DPPH (1,1-diphenyl-2-picrylhydrazyl). Results showed that irradiation source and dose generally significantly (p < 0.05) affected all the phytochemical compositions of sorghum grains studied in a non-dose dependent manner either by increasing or decreasing the levels. Gamma irradiation had the highest effects on the antioxidant activity of the sorghum grains as compared to the high and low energy electron beams.
AbdelaleemMAElbassionyKRA (2020) Evaluation of phytochemicals and antioxidant activity of gamma irradiated quinoa (Chenopodium quinoa). Brazilian Journal of Biology81: 806–813.
2.
AfifyAE-MMREl-BeltagiHSEl-SalamSMA, et al. (2012) Biochemical changes in phenols, flavonoids, tannins, vitamin E, carotene and antioxidant activity during soaking of three white sorghum varieties. Asian Pacific Journal of Tropical Biomedicine2: 203–209.
3.
AlothmanMBhatRKarimAA (2009) Effects of radiation processing on phytochemicals and antioxidants in plant produce. Trends in Food Science & Technology20(5): 201–212.
4.
AmiriMArabMSadrabadEK, et al. (2023) Kgeffect of gamma irradiation treatment on the antioxidant activity, phenolic compounds and flavonoid content of common buckwheat. Radiation Physics and Chemistry212: 111127.
5.
AwikaJMRooneyLW (2004) Sorghum phytochemicals and their potential impact on human health. Phytochemistry65(9): 1199–1221.
6.
AwikaJMRooneyLWWuX, et al. (2003) Screening methods to measure antioxidant activity of sorghum (Sorghum bicolor) and sorghum products. Journal of Agricultural and Food Chemistry51(23): 6657–6662.
7.
BakariHDjomdi, RubenZF, et al. (2023) Sorghum (Sorghum bicolor) and its main parts (by-products) as promising sustainable sources of value-added ingredients. Waste and Biomass Valorization14(4): 1023–1044.
8.
BrohanMJerkovicVCollinS (2011) Potentiality of red sorghum for producing stilbenoid-enriched beers with high antioxidant activity. Journal of Agricultural and Food Chemistry59: 4088–4094.
9.
ChávezDWAscheriJLCarvalhoCWGodoyRLPachecoS (2017) Sorghum and roasted coffee blends as a novel extruded product: Bioactive compounds and antioxidant capacity. Journal of Functional Foods29: 93–103.
10.
DasNGhosh DharDDharP (2023) Editing the genome of common cereals (rice and wheat): Techniques, applications, and industrial aspects. Molecular Biology Reports50(1): 739–747.
11.
de OliveiraKGQueirozVAVde Almeida CarlosL, et al. (2017) Effect of the storage time and temperature on phenolic compounds of sorghum grain and flour. Food Chemistry216: 390–398.
12.
DixitAKBhatnagarDKumarV, et al. (2010) Gamma irradiation induced enhancement in isoflavones, total phenol, anthocyanin and antioxidant properties of varying seed coat colored soybean. Journal of Agricultural and Food Chemistry58(7): 4298–4302.
13.
DykesLRooneyWLRooneyLW (2013) Evaluation of phenolics and antioxidant activity of black sorghum hybrids. Journal of Cereal Science58: 278e283.
14.
DykesLSeitzLMRooneyWL, et al. (2009) Flavonoid composition of red sorghum genotypes. Food Chemistry116(1): 313–317.
15.
Espitia-HernándezPChavez GonzalezMLAscacio-ValdésJA, et al. (2022) Sorghum (Sorghum bicolor L.) as a potential source of bioactive substances and their biological properties. Critical Reviews in Food Science and Nutrition62(8): 2269–2280.
16.
Farkhad SHosseiniA (2020) Effect of gamma irradiation on antioxidant potential, isoflavone aglycone and phytochemical content of soybean (Glycine max L. Merrill) cultivar Williams. Journal of Radioanalytical and Nuclear Chemistry324: 497–505.
17.
FrankowskiJPrzybylska-BalcerekAStuper-SzablewskaK (2022) Concentration of pro-health compound of Sorghum grain-based foods. Foods11(2): 216.
18.
Gaytán-MartínezMCabrera-RamírezÁHMorales-SánchezE, et al. (2017) Effect of nixtamalization process on the content and composition of phenolic compounds and antioxidant activity of two sorghums varieties. Journal of Cereal Science77: 1–8.
19.
GirardALAwikaJM (2018) Sorghum polyphenols and other bioactive components as functional and health promoting food ingredients. Journal of Cereal Science84: 112–124.
20.
GryczkaUMadureiraJVerdeSC, et al. (2021) Determination of pepper microbial contamination for low energy e-beam irradiation. Food Microbiology98: 103782.
21.
Guajardo-FloresS, 2008. Evaluation of Anticancer Potential of Sorghums with Different Genetic Characteristics and Levels of Phenolic Compounds. Ph.D. dissertation. Food Science and Technology. Texas A&M University, College Station, TX.
22.
HahnDHFaubionJMRooneyLW (1983) Sorghum phenolic acids, their high performance liquid chromatography separation and their relationship.
23.
HassanSM (2023) Nutritional, functional and bioactive properties of sorghum (Sorghum Bicolor I. Moench) with its future outlooks: A review. Open Journal of Nutrition and Food Sciences 5(1): 1030.
24.
HassanSAhmadNAhmadT, et al. (2019) Microwave processing impact on the phytochemicals of sorghum seeds as food ingredient. Journal of Food Processing and Preservation43(5): e13924.
25.
HithamaniGSrinivasanK (2014) Bioaccessibility of polyphenols from wheat (Triticum aestivum), sorghum (Sorghum bicolor), green gram (Vigna radiata), and chickpea (Cicer arietinum) as influenced by domestic food processing. Journal of Agricultural and Food Chemistry62: 11170–11179.
26.
HwangKEHamYKSongDH, et al. (2021) Effect of gamma-ray, electron-beam, and X-ray irradiation on antioxidant activity of mugwort extracts. Radiation Physics and Chemistry186: 109476.
27.
JaiswalVRawatKPSGuptaAD, et al. (2021) Comparison of starch characteristics from pigmented and non-pigmented sorghum cultivars before and after electron beam irradiation. Starch-Stärke73(3–4): 2000143.
28.
KangXGaoWCuiB, et al. (2023) Structure and genetic regulation of starch formation in sorghum (Sorghum bicolor (L.) Moench) endosperm: A review. International Journal of Biological Macromolecules239: 124315.
29.
KeyataEOTolaYBBultosaG, et al. (2023) Bioactive compounds, antioxidant capacity, functional and sensory properties of optimized complementary weaning flour processed from sorghum, soybean, and karkade (Hibiscus sabdariffa L.) seeds. Scientific African19: e01457.
30.
KhalidWAliAArshadMS, et al. (2022) Nutrients and bioactive compounds of Sorghum bicolor L. Used to prepare functional foods: A review on the efficacy against different chronic disorders. International Journal of Food Properties25(1): 1045–1062.
31.
KhoddamiAMessinaVVadabalija VenkataK, et al. (2023) Sorghum in foods: Functionality and potential in innovative products. Critical Reviews in Food Science and Nutrition63(9): 1170–1186.
32.
KhoddamiATruongHHLiuSY, et al. (2015) Concentrations of specific phenolic compounds in six red sorghums influence nutrient utilisation in broiler chickens. Animal Feed Science and Technology210: 190e199.
33.
LamLPYWangLLuiAC, et al. (2023) Flavonoids in major cereal grasses: Distribution, functions, biosynthesis, and applications. Phytochemistry Reviews22(5): 1–40.
34.
LiZZhaoXZhangX, et al. (2021) Bioactive compounds and biological activities of sorghum grains. Foods10(11): 2868.
35.
LiZZhaoXZhangX, et al. (2022) The effects of processing on bioactive compounds and biological activities of sorghum grains. Molecules27(10): 3246.
36.
LohaniUCMuthukumarappanK (2020) Influence of fermentation followed by ultrasonication on functional properties of sorghum extrudates. Journal of Food Process Engineering43(6): e13390.
37.
LuoXCuiJZhangH, et al. (2018) Subcritical water extraction of polyphenolic compounds from sorghum (Sorghum bicolor L.) bran and their biological activities. Food Chemistry262: 14–20.
38.
LuoXDuZYangK, et al. (2021) Effect of electron beam irradiation on phytochemical composition, lipase activity and fatty acid of quinoa. Journal of Cereal Science98: 103161.
39.
MalathiVMKanikaSJinuJ, et al. (2024) 43 Phenolic phytochemicals from Sorghum, millets, and pseudocereals and their role in human health. In: Nutriomics of Millet Crops. Boca Raton: CRC Press, 43–80.
40.
MawoumaSConduracheNNTurturicăM, et al. (2022) Chemical composition and antioxidant profile of sorghum (Sorghum bicolor L. Moench) and pearl millet (Pennisetum glaucum (L.) R. Br.) grains cultivated in the far-North region of Cameroon. Foods11(14): 2026.
41.
MeenaKVisaradaKBRSMeenaDK (2022) Sorghum bicolor (L.) Moench a multifarious crop-fodder to therapeutic potential and biotechnological applications: A future food for the millennium. Future Foods6: 100188.
42.
MohamedHIFawziEMBasitA, et al. (2022a) Sorghum: Nutritional factors, bioactive compounds, pharmaceutical and application in food systems: A review. Phyton91(7): 1303.
43.
MohamedLKSuliemanMAYagoubAEA, et al. (2022b) Changes in phytochemical compounds and antioxidant activity of two irradiated sorghum (Sorghum bicolor (L.) Monech) cultivars during the fermentation and cooking of traditional Sudanese Asida. Fermentation8(2): 60.
44.
N’DriDMazzeoTZaupaM, et al. (2013) Effect of cooking on the total antioxidant capacity andphenolic profile of some whole-meal African cereals. Journal of the Science of Food and Agriculture93: 29–36.
45.
NguyenNXUthairatanakijALaohakunjitN, et al. (2022) Effects of gamma irradiation dose and short-term storage on phytochemicals, antioxidants, and textural properties of boiled ‘Tainan 9’peanuts. International Journal of Food Science & Technology57(6): 3771–3782.
46.
OclooFCOdaiBTDarfourB, et al. (2024) Microbial quality and Aflatoxin levels of sorghum grains (Sorghum bicolor) irradiated with gamma rays, low energy electron beam (LEEB) and high energy electron beam (HEEB). Radiation Physics and Chemistry216: 111474.
47.
PessatoTBDe MoraisFPDe CarvalhoNCFigueiraACMFernandesLGRZollnerRDLNettoFM (2018) Protein structure modification and allergenic properties of whey proteins upon interaction with tea and coffee phenolic compounds. Journal of Functional Foods51: 121–129.
48.
PinheiroSSAnunciaçãoPCde Morais CardosoL, et al. (2021) Stability of B vitamins, vitamin E, xanthophylls and flavonoids during germination and maceration of sorghum (Sorghum bicolor L.). Food Chemistry345: 128775.
49.
Przybylska-BalcerekAFrankowskiJStuper-SzablewskaK (2018) Bioactive compounds in sorghum. European Food Research and Technology245: 1075–1080.
50.
Przybylska-BalcerekAFrankowskiJStuper-SzablewskaK (2019) Bioactive compounds in sorghum. European Food Research and Technology245: 1075–1080.
51.
PuniaHTokasJMalikA, et al. (2021) Characterization of phenolic compounds and antioxidant activity in sorghum [Sorghum bicolor (L.) Moench] grains. Cereal Research Communications49(3): 343–353.
52.
RamadanBRSorourMAKelanyMA (2012) Changes in total phenolics and DPPH scavenging activity during domestic processing in some cereal grains. Annals. Food Science and Technology.13: 190–196.
53.
RatherMAThakurRHoqueM, et al. (2023) Sorghum (Sorghum bicolor): Phytochemical composition, bio-functional, and technological characteristics. In: Nutri-Cereals. CRC Press, 57–89.
54.
SantanaÁLPetersonJPerumalR, et al. (2023) Post acid treatment on pressurized liquid extracts of sorghum (Sorghum bicolor L. Moench) grain and plant material improves quantification and identification of 3-deoxyanthocyanidins. Processes11(7): 2079.
55.
ShawrangPSadeghiAABehgarM, et al. (2011) Study of chemical compositions, anti-nutritional contents and digestibility of electron beam irradiated sorghum grains. Food Chemistry125(2): 376–379.
56.
ShenHHouYXiM, et al. (2022) Electron beam irradiation enhanced extraction and antioxidant activity of active compounds in green walnut husk. Food Chemistry373: 131520.
57.
ShiZLiuYHuZ, et al. (2022b) Effect of radiation processing on phenolic antioxidants in cereal and legume seeds: A review. Food Chemistry396: 133661.
58.
ShiDYinCFanX, et al. (2022a) Effects of ultrasound and gamma irradiation on quality maintenance of fresh Lentinula edodes during cold storage. Food Chemistry373: 131478.
59.
SinghSHabibMKumarY, et al. (2025) Structural reorganization and antioxidant enhancement of sorghum kafirin isolates induced by gamma irradiation. Food Chemistry486: 144647.
60.
SultanNWaniIAMasoodiFA (2018) Moisture mediated effects of γ-irradiation on physicochemical, functional, and antioxidant properties of pigmented brown rice (Oryza sativa L.) flour. Journal of Cereal Science79: 399–407.
61.
VanamalaJKMasseyARPinnamaneniSR, et al. (2018) Grain and sweet sorghum (Sorghum bicolor L. Moench) serves as a novel source of bioactive compounds for human health. Critical Reviews in Food Science and Nutrition58(17): 2867–2881.
62.
Van HungPMoritaN (2008) Distribution of phenolic compounds in the graded flours milled from whole buckwheat grains and their antioxidant capacities. Food Chemistry109(2): 325–331.
63.
VermaMSharmaPGourVS, et al. (2016) Moisture-mediated effects of γ-irradiation on antioxidant properties of mung bean (Vigna radiate L.) cultivars. Innovative Food Science & Emerging Technologies34: 59–67.
64.
WeremfoAAdulleyFDabieK, et al. (2022) Optimization of ultrasound-assisted extraction of phenolic antioxidants from Turkey berry (Solanum torvum Sw) fruits using response surface methodology. Journal of Applied Research on Medicinal and Aromatic Plants30: 100387.
65.
WuLHuangZQinP, et al. (2013) Effects of processing on phytochemical profiles and biological activities for production of sorghum tea. Food Research International53(2): 678–685.
66.
WuGJohnsonSKBornmanJF, et al. (2016) Growth temperature and genotype both play important roles in sorghum grain phenolic composition. Scientific Reports6(1): 21835.
67.
WuGJohnsonSKBornmanJF, et al. (2017) Changes in whole grain polyphenols and antioxidant activity of six sorghum genotypes under different irrigation treatments. Food Chemistry214: 199–207.
68.
WuYWangYLiuZ, et al. (2023) Extraction, identification and antioxidant activity of 3-deoxyanthocyanidins from Sorghum bicolor L. Moench cultivated in China. Antioxidants12(2): 468.
69.
XuXBeanSWuX, et al. (2022) Effects of protein digestion on in vitro digestibility of starch in sorghum differing in endosperm hardness and flour particle size. Food Chemistry383: 132635.
70.
YangLAllredKFDykesL, et al. (2015) Enhanced action of apigenin and naringenin combination on estrogen receptor activation in non-malignant colonocytes: Implications on sorghum-derived phytoestrogens. Food & Function6(3): 749–755.
71.
ZhangXWangLChenZ, et al. (2020) Effect of high energy electron beam on proteolysis and antioxidant activity of rice proteins. Food & Function11(1): 871–882.
72.
ZhuFCaiYZBaoJ, et al. (2010) Effect of γ-irradiation on phenolic compounds in rice grain. Food Chemistry120(1): 74–77.
