Note: Inhibition of Pathogenic Bacteria by Essential Oils at Different Concentrations

Abstract

The antimicrobial effect of 11 selected Turkish spice essential oils was investigated against seventeen pathogenic bacteria. The antimicrobial activity of the essential oils of six spices (cumin, fennel, laurel, mint, marjoram, oregano, pickling herb, sage, savory, thyme (black) and thyme) was tested at four concentrations (0.2, 0.4, 1 and 2%) on various microorganisms (E. aerogenes, E. coli, E. coli O157:H7, K. pneumoniae, P. vulgaris, S. enteritidis, S. gallinarum, S. typhimurium, S. aureus, Y. enterocolitica, A. hydrophila, C. xerosis, M. luteus, M. smegmatis, E. feacalis, P. aeruginosa and P. fluorescens). All preparations showed antibacterial activity against at least one or more bacteria. The inhibitory effect of the essential oils was evaluated through paper disc diffusion method. In general, the essential oils at 1 and 2% levels were effective. The most active essential oils were marjoram, thyme and oregano. According to the results, the studied essential oils potentially might be used as antibacterial agents to prevent the spoilage of food products, although further research is needed.

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References

Akgül A. and Kivanç M. (1988). Inhibitory effects of six Turkish thym-like spices on some common foodborne bacteria . Die Nahrung 32: 201-203 .

Akgül A. (1989). Antimicrobial activity of black cumin (Nigella sativa L.) essential oil . Journal of Faculty of Pharmacology Gazi 6: 63-68 .

Aureli P., Costantini A. and Olea S. (1992). Antimicrobial activity of some plant essential oils against Listeria monogytogenes . Journal of Food Protection 55(5): 344-348 .

Baytop T. (1984). Treatment with plants in Turkey. Publication No 3255. Istanbul, Turkey: Istanbul University .

Deans S.G. and Svoboda K.P. (1989) Antimicrobial activity of summer savory (Satureja hortensis L.) essential oil and its constituents . Journal of Horticultural Science 64: 205-210 .

Deans S.G. and Svoboda K.P. (1990). The microbial properties of marjoram (Origanum majorana L.) volatile oil . Flavour Fragrance Journal 5: 187-190 .

Dorman H.J.D. and Deans S.G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils . Journal of Applied Microbiology 88: 308-316 .

Farag R.S., Daw Z.Y., Hewedi F.M. and El-Baroty G.S.A. (1989). Antimicrobial activity of some Egyptian spice essential oils . Journal of Food Protection 52: 665-667 .

Hall M.A. and Maurer A.J. (1986). Spice extracts, Lauricidin and Propylene Glycol as Inhibitors of Clostridium botulinum in Turkey Frankfurter Slurries . Poultry Science 65: 1167-1171 .

10.

Hammer K.A., Carson C.F. and Riley T.V. (1999). Antimicrobial activity of essential oils and other plant extracts . Journal of Applied Microbiology 86: 985-990 .

11.

Hsieh P.C., Mau J.L. and Huang S.H. (2001). Antimicrobial effect of various combinations of plant extracts . Food Microbiology 18: 35-43 .

12.

Huntanen C.N. (1980). Inhibition of Clostridium botulinum by spice extracts and aliphatic alcohols . Journal of Food Protection 43: 195-196 .

13.

Ilçim A., Diğrak M. and Bağci E. (1998). The investigation of antimicrobial effect of some plant extract . Turkish Journal of Biology 22: 119-125 .

14.

Kar A. and Jain S.R. (1971). Investigations on the antibacterial activity of some Indian indigenous aromatic plants . The Flavour Industry 2: 111-113 .

15.

Kelmanson J.E., Jäger A.K. and van Staden J. (2000). Zulu medicinal plants with antibacterial activity . Journal of Ethnopharmacology 69: 241-246 .

16.

Kivanç M. and Akgül A. (1986). Antibacterial activities of essentials oils from Turkish spices and citrus . Flavour and Fragrance Journal 1: 175-179 .

17.

Marino M., Bersani C. and Comi G. (2001). Impedance measurements to study the antimicrobial activity of essential oils from Lamiaceae and Compositae . International Journal of Food Microbiology 67: 187-195 .

18.

Nkanga E.J. and Uraih N. (1981). Prevalence of Staphylococcus aureus in meat samples from traditional markets in Benin City, Nigeria and possible control by use of condiments . Journal of Food Protection 44(4): 18-27 .

19.

Özcan M. and Boyraz N. (2000). Antifungal properties of some herb decoctions . European Food Research Technology 212: 86-88 .

20.

Özcan M. and Erkmen O. (2001). Antimicrobial activity of the essential oils of Turkish plant spices . European Food Research Technology 212: 658-660 .

21.

Sağdiç O. and Özcan M. (2002). Antibacterial activity of Turkish spice hydrosols. Food Control (in press).

22.

Sağdiç O., Kuşçu A., Özcan M. and Özçelik S. (2002) Effects of Turkish spice extracts at various concentrations on the growth of Escherichia coli O157:H7 . Food Microbiology 19: 473-480 .

23.

Samy R.P. and Ignacimuthu S. (2000). Antibacterial activity of some folklore medicinal plants used by tribals in Western Ghats of India . Journal of Ethnopharmacology 69: 63-71 .

24.

SAS (1998). The SAS® system for Windows V7. Cary, NC, USA: SAS Institute Inc.

25.

Skandamis P.N. and Nychas G.J.E. (2000). Development and evaluation of a model predicting the survival of escherichia coli O157:117 NCTC 12900 in homemade eggplant salad at various temperatures, pHs, and oregano essential oil concentrations . Applied Environmental Microbiology 66: 1646-1683 .