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Abstract

Background

To examine the prognostic relevance of pan-immune-inflammation value (PIV) in locally advanced nasopharyngeal carcinomas (LA-NPC) patients treated with concurrent chemoradiotherapy (CCRT) definitively.

Methods

We used receiver operating characteristic (ROC) curve analysis to determine an optimal PIV cutoff that could effectively divide the patient cohort into two distinct groups based on distant metastasis-free (DMFS) and overall survival (OS) results. For this purpose, receiver operating characteristic (ROC) curve analysis was employed. Our primary and secondary endpoints were to investigate the potential correlations between pre-CCRT PIV measurements and post-CCRT OS and DMFS outcomes, respectively.

Results

This retrospective cohort study included 179 LA-NPC patients. The optimal PIV cutoff was 512 (area under the curve: 74.0%; sensitivity: 70.8%, specificity: 68.6%; J-index: 0.394) in ROC curve analysis, creating two patient groups: Group-1: PIV < 512 (N = 108); vs Group-2: PIV ≥ 512 (N = 71). In the comparative analysis, although there were no significant differences between the two groups regarding the patient, disease, and treatment characteristics, the PIV ≥ 512 group had significantly poorer median OS [74.0 months vs not reached yet (NR); HR: 2.81; P < 0.001] and DMFS (27.0 months vs NR; HR: 3.23; P < 0.001) than the PIV < 512 group. Apart from PIV ≥ 512, the N2-3 nodal stage and ≥ 5% weight loss within the preceding 6 months were significant predictors of unfavorable outcomes for DMFS (P < 0.05 for each) and OS (P < 0.05 for each) in univariate analyses. The results of the multivariate analysis showed that each of the three variables had independent negative impacts on both DMFS and OS outcomes (P < 0.05 for each).

Conclusions

The present findings indicate that PIV, which classifies these patients into two groups with significantly different DMFS and OS, might be a potent prognostic biological marker for LA-NPC patients.

Keywords

biomarker inflammation immune response nasopharyngeal cancer chemoradiotherapy survival

Introduction

Nasopharyngeal carcinomas (NPCs) are highly aggressive cancers that develop from the squamous epithelial cells lining the lateral wall of the nasopharynx, which are most prevalent in endemic areas like China and northern Africa.¹ NPCs have distinct epidemiology, pathology, clinical characteristics, and treatment outcomes compared to other head and neck cancers. The current standard treatment options for medically fit, locally advanced LA-NPC patients consist of concurrent chemoradiotherapy (CCRT) with intensity-modulated radiotherapy (IMRT) or induction chemotherapy followed by IMRT-based CCRT.^2-4 Despite significant advancements in diagnostic and staging tools and the successful implementation of concurrent chemotherapy and IMRT, which have resulted in loco-regional tumor control rates exceeding 90%,^2,3 up to 20% of all LANPC cases still experience distant metastasis (DM) during the course of the disease.^2-5

The TNM (tumor-node-metastasis) staging system is the most comprehensive current framework for predicting the prognosis of patients presenting with locoregionally advanced nasopharyngeal carcinoma (LA-NPC). However, clinical outcomes may significantly vary among patients even if they receive similar anti-cancer interventions for identical disease stages, which may stem from inherent tumor- and host-related biological disparities.^6,7 These biological differences and their unpredictable impact on clinical results underscore the necessity for novel and efficacious biomarkers to complement conventional TNM staging for more accurate prognostic classification of these patients. Persistent systemic inflammation and deficient antitumor immunity are crucial factors that trigger various phases of cancer progression, including cellular transformation, tumor cell survival, growth, invasion, extravasation, metastatic dissemination, and immune evasion.⁸ Consequently, several combinations of blood-borne monocytes, platelets, neutrophils, and lymphocytes have been studied to ascertain their prognostic relevance in NPC patients. Multiple research studies consistently show that specific biomarkers involving the ratio of two or three types of cells, such as the neutrophil-to-lymphocyte ratio (NLR),^5,9 monocyte-to-lymphocyte ratio (MLR),⁹ platelet-to-lymphocyte ratio (PLR),^10,11 systemic immune-inflammation index (SII),¹² and systemic immune response index (SIRI),¹³ can robustly predict the prognosis of patients with LA-NPC who receive systemic chemotherapy or concurrent chemoradiotherapy (CCRT).

In 2020, Fucà and colleagues introduced a novel comprehensive biological marker, the pan-immune-inflammation value (PIV), which integrates the platelet, monocyte, neutrophil, and lymphocyte counts.¹⁴ The authors investigated its prognostic utility in 438 stage IV colorectal cancer patients who received systemic chemotherapy. The findings of the investigation revealed that patients with higher PIV exhibited inferior progression-free survival (PFS) (P < 0.001) and overall survival (OS) (P < 0.001) in comparison to those with low PIV. After this highly esteemed investigation, numerous researchers have confirmed the predictive significance of novel PIV across a range of solid malignancies, such as malignant melanomas,¹⁵ Merkel cell,¹⁶ colorectal,^17,18 breast,^19-21 esophageal,²² and non-small-cell and small-cell lung carcinomas.^23,24 Surprisingly, despite the abundance of reliable basic and clinical data for other solid cancers, the prognostic essence of PIV has never been explored in radically treated LA-NPC patients. Motivated by the noteworthy discoveries in other tumor sites, we endeavored to ascertain whether pretreatment PIV values held a prognostic significance in LANPCs receiving definitive CCRT.

Patients and Methods

Study Design and Participants

The reporting of this study conforms to REMARK guidelines.²⁵ The present investigation was a retrospective cohort study conducted at the Department of Radiation Oncology of the Baskent University Medical Faculty with a single-center design. Records of LA-NPC patients who had CCRT at our facility between June 2010 and December 2019 were reviewed retrospectively. The study’s eligibility criteria encompassed the following requirements: patients aged 18 to 80 years, an Eastern Cooperative Oncology Group (ECOG) performance score of 0-2, and a BMI of at least 18.5 kg/m². Participants had to have accessible pretreatment clinical ear-nose-throat (ENT) examinations, head and neck magnetic resonance imaging (MRI) scans, fluorodeoxyglucose-positron emission computerized tomography (PET-CT), clinical/radiological T3-4N0-3M0 or T1-4N1-3M0 disease according to the AJCC 8^th edition, documented non-keratinizing or undifferentiated squamous cell histology, no history of other cancers, chemotherapy or RT, infectious diseases, or immune suppressive drug use in the past 30 days before the commencement of CCRT. Patients need to have received one or more cycles of platinum-based concurrent chemotherapy. Accessible RT and chemotherapy charts, available baseline complete blood count test records, records of follow-up ENT examinations, and MRI and PET-CT scans were also required.

Ethics and Consent

The Institutional Review Board of the Baskent University Medical Faculty approved the study design before collecting any patient data (Project no: D-KA-2058), which adhered to the exact specifications stipulated in the Declaration of Helsinki and its subsequent updates. Participants who met the eligibility requirements for the study gave their informed consent for the collection and analysis of blood samples and pathological specimens, and for the publication of the research results through academic channels. Preliminary results of these data were published as an oral presentation at the 8^th International Black Sea Coastline Countries Scientific Research Conference, August 29-30, 2022, Sofia, Bulgaria.²⁶

Chemoradiotherapy Protocol

All study participants underwent definitive CCRT utilizing previously documented dosages of RT and chemotherapy.²⁷ In brief, we employed intensity-modulated radiotherapy (IMRT) administered in daily fractions over 7 weeks, 5 days per week. Concurrently, chemotherapy was delivered with cisplatin dosages of 75-80 mg/m² at 3-week intervals. After CCRT, adjuvant chemotherapy consisted of two cycles of cisplatin-based regimens at 3-week intervals were advised if deemed tolerable, contingent upon treatment protocol approval. Additionally, appropriate supportive care measures, including the provision of antiemetic medications and oral/enteral nutritional supplementation, were administered as clinically indicated.

Measurement of Pan-Immune-Inflammation Value

The pre-CCRT PIV was estimated for each patient using the original Fucà formula: PIV = [P × M × N] ÷ L, with P, M, N, and L representing the counts (per microliter) of platelets, monocytes, neutrophils, and lymphocytes acquired on day 1 of CCRT.¹⁴

Response Assessment

Although the research design was retrospective, the CCRT response evaluations were prospective under the norms of our institution. In the initial 2-year and 3- to 5-year periods of CCRT, patients underwent evaluations every 3-month and every 6-month intervals, respectively. Then, the patients underwent annual or more frequent assessments as deemed necessary based on clinical indications. A meticulous endoscopic ENT examination was conducted at each visit to identify potential local or regional tumor recurrences and the likelihood of second primary tumors. In addition, PET-CT scans were acquired to evaluate the probable distant metastasis (DM) and determine the type of treatment response. The PET Response Criteria for Solid Tumors (PERCIST) was the standard tool for response evaluations. When a complete metabolic response was ascertained, head and neck MRI and/or CT scans were used instead of PET-CT scanning from that point on. Supplementary imaging modalities were exclusively utilized for the detection of suspected lesions or the reevaluation of recurrent tumors.

Clinical Endpoints and Statistics

This study’s primary objective was to ascertain if there was a correlation between the pre-CCRT PIV and OS (the interval between the commencement of CCRT and the death or last follow-up records. The secondary objective was to evaluate the same relationship with DM-free survival (DMFS: The duration between the initiation of CCRT and the occurrence of any distant or non-regional lymph node relapses, death, or the final visit. Continuous data were represented using medians and ranges, whereas frequency distributions characterized categorical variables. We used the student’s t-test, Chi-square test, or Spearman correlations to compare frequency distributions across different groups. We utilized receiver operating characteristic (ROC) curve analysis to evaluate the availability of an optimal pre-CCRT PIV cutoff value that would divide the entire study population into two groups with significantly different DMFS and OS results. Kaplan-Meier curves and log-rank tests were employed to explore the potential interactions between the tested risk factors and the results of DMFS and OS. The Cox proportional hazards model was applied to carry out multivariate analyses to assess the independence of variables that demonstrated univariate significance. All comparisons between groups were conducted using a two-tailed test, and statistical significance was determined at a level of P < 0.05. The statistical analyses were conducted using version 26 of the Statistical Package for the Social Sciences (SPSS).

Results

The primary baseline patient and disease characteristics of the 179 patients who met the eligibility criteria are shown in Table 1. The median age of the participants was 59 years, with a range of 23 to 79 years. Most of the subjects were of the male gender, accounting for 78.2% of the population studied. Smoking and alcohol consumption history was present in 87.7% and 69.3% of the patients, respectively. A significant proportion of the participants exhibited advanced stages of the disease, with 83.8% presenting with T3-4 and 70.9% with N2-3. Weight loss (WL) ≥ 5% within the past 6 months) was observed in 38.0% of the study participants. CCRT had a relatively favorable tolerance profile without any instances of grade 5 toxicity, and 124 (69.3%), 39 (21.8%), and 16 (8.9%) patients completed the prescribed 3, 2, and 1 of the concurrent chemotherapy cycles, respectively. Furthermore, a total of 127 (70.9%) patients could receive either 2 (N = 106; 53.6%) or 1 (N = 21; 17.6%) cycles of adjuvant chemotherapy after the CCRT phase, as outlined in Table 2. Throughout the follow-up, only 2 (1.1%) incidents of late treatment-related death occurred: 1 case of an intractable carotid artery blow-out and 1 case of aspiration pneumonia caused by a tracheoesophageal fistula in the eighth and sixteenth months of CCRT, respectively.

Table 1.

Pretreatment Patients and Disease Characteristics.

Characteristics	Whole cohort (N = 179)	PIV < 512 (N = 108)	PIV ≥ 512 (N = 71)	P-value
Median age, years (range)	59 (23-79)	60 (23-79)	58 (34-78)	0.93
Age group (N; %)
≥70 years	47 (26.3)	31 (28.7)	16 (22.5)	0.32
<70 years	132 (73.7)	77 (71.3)	55 (77.5)	0.32
Gender (N; %)
Male	140 (78.2)	85 (78.7)	55 (77.5)	0.91
Female	39 (21.8)	23 (21.3)	16 (22.5)	0.91
Smoking history (N; %)
Absent	22 (12.3)	13 (12.0)	9 (12.7)	0.86
Present	157 (87.7)	95 (88.0)	62 (87.3)	0.86
Alcohol consumption history (N; %)
Absent	55 (30.7)	35 (32.4)	20 (28.2)	0.47
Present	124 (69.3)	73 (67.6)	51 (71.8)	0.47
ECOG performance (N; %)
0	83 (46.4)	50 (46.3)	33 (46.5)	0.95
1	96 (53.6)	58 (53.7)	38 (53.5)	0.95
WL over past 6 months (N; %)
<5%	111 (62.0)	78(64.8)	33 (46.4)	0.003
≥5%	68 (38.0)	30 (35.2)	38 (53.6)	0.003
WHO histology (N; %)
2	24 (13.4)	14 (13.0)	10 (14.1)	0.84
3	155 (86.6)	94 (87.0)	61 (85.9)	0.84
T-stage (N; %)
2	29 (16.2)	18 (16.7)	11 (15.5)	0.78
3-4	150 (83.8)	90 (83.3)	60 (84.5)	0.78
N-stage (N; %)
0-1	52 (29.1)	31 (28.7)	21 (29.6)	0.80
2-3	127 (70.9)	77 (71.3)	50 (70.4)	0.80
Clinical stage (N; %)
II-III	103 (57.5)	68 (63.0)	35 (49.3)	0.16
IVA-B	76 (42.5)	40 (37.0)	36 (50.7)	0.16

Abbreviations: PIV: Pan-immune-inflammation value; ECOG: Eastern Cooperative Oncology Group; WL: Weight loss; WHO: World Health Organization; T-stage: Tumor stage; N-stage: Nodal stage.

Table 2.

Treatment and Survival Results per Pan-Immune-Inflammation Value Group.

Outcome	Whole cohort (N = 179)	PIV < 512 (N = 108)	PIV ≥ 512 (N = 71)	P-value
Concurrent chemotherapy cycles (N; %)
1-2	16 (8.9)	6 (5.6)	10 (14.1)	0.041
3	163 (91.1)	102 (94.4)	61 (85.9)	0.041
Adjuvant chemotherapy cycles (N; %)
0	52 (28.8)	22 (20.4)	30 (42.3)	0.007
1-2	127 (71.2)	86 (79.6)	41 (57.7)	0.007
Treatment related late fatalities (N; %)	2 (1.1)	1 (0.9)	1 (1.4)	0.78
OS
Median (mo)	NR	NR	74.0	<0.001
5-year (%)	78.1	91.3	57.9
10-year (%)	67.9	89.7	27.8
DMFS
Median (mo)	NR	NR	27.0	<0.001
5-year (%)	56.4	69.1	36.8
10-year (%)	50.2	65.3	23.3

Abbreviations: PIV: Pan-immune-inflammation; OS: Overall survival; DMFS: Distant metastasis-free survival.

We searched to find the optimal PIV cut-off values that could effectively stratify the study cohort into two groups with significantly different prognoses. ROC curve analyses yielded two ideal cut-off points, namely 512 [area under the curve (AUC): 74.0%; sensitivity: 70.8%; specificity: 68.6%; J-index: 0.394] and 508 (AUC: 83.3%; sensitivity: 72.9%; specificity: 70.7%; J-index: 0.436), which were found to be significantly correlated with the OS and DMFS results, respectively (Figure 1). Based on the proximity of the two cut-off values, a decision was made to select 512 as the PIV cut-off point for categorizing patients into two distinct groups: Group 1: PIV < 512 (N = 108) and Group 2: PIV ≥ 512 (N = 71). Considering the pretreatment and treatment characteristics, WL ≥ 5% was found to be meaningfully more frequent in the PIV ≥ 512 cohort than in the PIV < 512 cohort (53.6% vs 35.2%; P = 0.003) (Table 1). Similarly, the PIV ≥ 512 patient groups could tolerate significantly fewer chemotherapy cycles during the concurrent (P = 0.041) and adjuvant (P = 0.007) treatment phases (Table 2).

Figure 1.

Results of receiver operating characteristic curve analyses: (a) Distant metastasis-free survival, (b) Overall survival.

After a median follow-up period of 85.7 months (95% CI: 67.9-103.5 months), 134 out of 179 participants (74.9%) were still alive, and 96 (53.6%) were free from the disease. Among the 45 deaths, 30 (66.7%) were due to cancer and its treatment, with 28 cases attributed to uncontrolled LA-NPC progression and 2 cases due to late toxicities. Non-NPC factors caused the remaining 15 deaths (33.3%). Locoregional control (LRC) was attained in 161 (89.9%) patients. Although not statistically significant, the group with PIV ≥ 512 showed a numerically lower LRC rate compared to the PIV < 512 group (87.9% vs 93.0%; P = 0.23). Throughout the follow-up period, DM was identified in 66 patients (36.7%). Notably, the PIV 512 group had a significantly higher incidence of DM than the PIV 512 group (53.5% vs 23.1%; P < 0.001).

The median DMFS and median OS times were not reached during the final analysis for the whole research cohort, as indicated by the Kaplan-Meier survival estimates. The DMFS and OS rates at 10 years were 50.2% and 67.9%, respectively (Table 2). When the two PIV cohorts were compared, the PIV ≥ 512 patients had significantly poorer median DMFS [27.0 months (95% CI: 14,3-39.7) vs not reached (NR); P < 0.001] and OS [74.0 months (95% CI: 42.7-99.3) vs NR; P < 0.001] durations than the PIV < 512 patients (Table 2 and Figure 2). As shown in Table 2, the 5-year and 10-year DMFS and OS estimates for the PIV ≥ 512 groups were also quantitatively inferior

Figure 2.

Comparative survival outcomes between the pan-immune-inflammation value groups, (a) Distant metastasis-free survival (b) Overall survival (dark blue line: low pan-immune-inflammation value; red line: high pan-immune-inflammation value).

In univariate analysis, we revealed that the N2-3 stage and the WL ≥ 5%, in addition to the PIV ≥ 512, were significant indicators of inferior DMFS (P 0.05 for each) and OS (P 0.05 for each) outcomes (Table 3). As shown in Table 3, subsequent multivariate analysis focusing on these variables revealed that each variable independently exerted an adverse effect on both DMFS (P < 0.05 for each) and OS outcomes (P < 0.05 for each).

Table 3.

Results of Uni- and Multivariate Analysis.

	OS			DMFS
Factor	Univariate P-value	Multivariate P-value	HR (range)	Univariate P-value	Multivariate P-value	HR
Gender (Male vs Female)	0.67	—	—	0.55	—	—
Age group (<70 vs ≥70 y)	0.48	—	—	0.39	—	—
ECOG (0 vs 1)	0.79	—	—	0.63	—	—
Smoking history (Absent vs Present)	0.18	—	—	0.21	—	—
Alcohol consumption (Absent vs Present)	0.29	—	—	0.26	—	—
WHO histology (2 vs 3)	0.83	—	—	0.89	—	—
Weight loss (≤5% vs >5%)	0.001	0.002	1.78 (1.43-2.18)	<0.001	<0.001	2.02 (1.74-2.49)
T-stage (2 vs 3-4)	0.27	—	—	0.32	—	—
N-stage (0-1 vs 2-3)	0.003	0.005	1.46 (1.21-1.84)	0.002	0.001	1.63 (1.28-2.11)
Concurrent chemotherapy cycles (3 vs 1-2)	0.21	—	—	0.19	—	—
Adjuvant chemotherapy cycles (1-2 vs 0)	0.27	—	—	0.22	—	—
PIV (<512 vs ≥ 512)	<0.001	<0.001	2.81 (2.18-3.67)	<0.001	<0.001	3.23 (2.56-4.07)

Abbreviations: OS: Overall survival; DMFS: Distant metastasis-free survival; HR: Hazard ratio; ECOG: Eastern Cooperative Oncology Group; WHO: World Health Organization; T-stage: Tumor stage; N-stage: Nodal stage; PIV: Pan-immune-inflammation.

Discussion

Identifying new biological markers in patients with LA-NPC who receive radical CCRT may enhance patient selection and customize treatment protocols. Consequently, given the lack of comparably designed antecedents, this retrospective study aimed to evaluate the prognostic value of the novel PIV in a cohort of 179 LA-NPC patients who received CCRT. The results revealed that LA-NPC patients with PIV ≥ 512 displayed decreased tolerance to the prescribed concurrent and adjuvant chemotherapy cycles. Furthermore, this patient group exhibited significantly inferior median and long-term DMFS and OS outcomes in comparison to those with PIV < 512. The significance of these findings lies in their indication of a direct association between a chronically compromised immune-inflammatory state and decreased tolerance to treatment protocol, as well as unfavorable clinical outcomes. This information could prove beneficial in precisely risk stratification of such patients and selecting the optimal individualized treatment modalities.

The highly inflammatory nature of NPC is attributed to the spatial arrangement of stromal cells within tumor compartments. These cells reside in proximity to NPC cells and exhibit physical contact with them, as opposed to being situated in the surrounding regions.²⁸ Additionally, NPCs show a higher level of stromal infiltration than most other solid tumors due to the abundance of secondary and tertiary lymphoid structures, creating a unique environment that promotes a significant influx of inflammatory cells and associated chemokines/cytokines.²⁹ While the precise mechanisms remain complex, it is plausible that these inflammatory cells may promote various aspects of tumor-cell behavior, including proliferation, survival, invasiveness, migration, and metastasis. Furthermore, they have the potential to increase resistance to apoptotic stimuli and anti-neoplastic therapies while also impeding the tolerability of chemotherapy and RT, thereby resulting in elevated rates of both acute and chronic toxicity.³⁰ Elevated levels of regulatory T-cells, myeloid-derived platelets, monocytes, and neutrophils can have both inflammatory and immunosuppressive effects, potentially reducing the effectiveness and tolerance of therapy. T-cells possess distinct antitumor immune and anti-inflammatory properties compared to other cell types, thereby enhancing treatment efficacy across various tumor categories, including LA-NPCs.²⁹ In this respect, our findings reasserted the existing fundamental and clinical evidence by highlighting that, patients in the PIV ≥ 512 group demonstrated a reduced ability to tolerate both concurrent (85.9% vs 94.4%; P = 0.041) and adjuvant (57.7% vs 79.6%; P = 0.007) chemotherapy cycles. This observation is consistent with prior research suggesting that patients diagnosed with LA-NPC and presenting an elevated inflammatory response are more susceptible to a range of severe complications, WL ≥ 5%, and decreased tolerance to chemotherapy and CCRT, even with the application of IMRT.^31-33 Similarly, studies conducted on transplant tumor models and clinical trials have demonstrated that myeloid-derived suppressor cells (MDSCs), which can originate from both neutrophils and monocytes, along with the NFkB secreted by them, can facilitate resistance to RT and chemotherapy, affirming the pivotal role of persistent inflammation in the development of treatment resistance.^34-36 In line with previous findings, our study has disclosed that subjects categorized under the PIV ≥ 512 group exhibited a statistically non-significant, yet quantitatively lower rate of local-regional control (87.9% vs 93.0%; P = 0.23) and a significantly elevated rate of DM (53.5% vs 23.1%; P < 0.001) in comparison to those allocated to the PIV < 512 group.

Several immune-inflammation indices, such as lymphocyte counts, NLR,⁹ PLR,¹⁰ MLR,¹¹ SII,¹² and SIRI,¹³ have previously been utilized to predict the prognosis of LA-NPC patients undergoing CCRT. However, our current study is the first to investigate the prognostic value of PIV, a novel comprehensive four-cell immune-inflammation index, in LA-NPC patients receiving radical CCRT. The study findings revealed that pretreatment PIV measurements were independently prognostic in the patient population under investigation. Specifically, a PIV value of ≥ 512 was found to be significantly associated with shorter median DMFS (27.0 months vs NR; P < 0.001) and OS (71.0 months vs NR; P < 0.001) times, as well as lower 5-year and 10-year survival rates (Table 2). Notwithstanding the difficulties associated with comparing studies that employ distinct methodologies and endpoints, our results appear to be harmonious with those of previous SIRI and SII investigations conducted on LA-NPCs and PIV research in other tumor locations.^12,13 Feng and colleagues discovered that a heightened pretreatment level of SIRI was the sole independent predictor that correlated with notably decreased durations of PFS (P < 0.001) and OS (P < 0.001) in a propensity score matching analysis (PSMA) that encompassed 417 NPC patients.¹² Likewise, in an earlier PSMA research, Jiang et al. concluded that high pretreatment SII values were strong and independent predictors of OS (P = 0.007) in a group of 327 NPC patients treated with either RT or CCRT.¹³ The novel four-cell-based PIV used here appears to be more comprehensive than either the three-cell-based SIRI or SII. It integrates platelets and monocytes simultaneously into its equation, which are absent in SIRI (PIV = Platelets × SIRI) and SII (PIV = Monocytes × SII). Thus, it is more resilient to physiological, nutritional, or therapeutic fluctuations at any given moment. Although PIV emerged as a novel biomarker for prognostic stratification of LA-NPC in our study, it has been well-established as a robust prognosticator in many tumor types since Fuca’s initial research was published in 2020.^14,37 Although additional research is required to confirm the findings presented in this study, given the compelling evidence currently available, it is reasonable to propose that novel PIV could serve as a dependable prognostic indicator for LA-NPC patients who have undergone radical CCRT.

We acknowledge that certain limitations constrain our research. First, the current findings may be susceptible to selection bias due to the retrospective nature of the cohort analysis, which was conducted at a single center with a relatively modest cohort size. Second, our results may not be universally applicable to all patients with LA-NPC, particularly those with an ECOG 2 performance score or those undergoing different RT or chemotherapy regimens, as our study exclusively included patients with an ECOG performance score of 0-1 who were staged and treated uniformly. Third, as components of PIV may fluctuate dramatically over time, the pretreatment PIV cutoff utilized in this investigation and the results reported may only represent a snapshot in time, as we did not collect additional data throughout or after the C-CRT course. Fourth, the absence of Epstein-Barr virus (EBV)–related parameters in most patients poses a challenge in correlating PIV groups with these parameters in our study. However, a recent investigation by Zhang and colleagues into the correlation between EBV and PIV statuses revealed no significant relationships between the two parameters.³⁸ Consequently, if EBV status does exert an influence on the PIV groups, it is likely to be negligible or non-existent.³³ And fifth, the lack of an internal and/or external validation cohort may have underrated specific crucial parameters, such as the well-established prognostic relevance of the presenting T-stage. Therefore, we concede these obstacles and advocate viewing current findings as hypothesis-generating rather than firm recommendations until additional data from well-designed future research addressing these constraints may validate them. Nevertheless, when viewed through the lens of Clark’s well-respected prognostic factor definition,³⁹ our findings appear to point to the novel PIV as a dependable prognosticator, as it is a biological parameter that is simple to obtain and compute, objectively measurable, replicable, inexpensive and provides data regarding the probable outcome of LA-NPC patients regardless of their clinical conditions.

Conclusion

Notwithstanding the limitations of a retrospective cohort study conducted at a single institution, our current findings emphasize the efficacy of the novel PIV as a dependable prognostic marker for patients with locally advanced nasopharyngeal carcinoma (LA-NPC). Given the PIV ≥ 512 cohort’s drastically reduced treatment tolerance and worse survival outcomes, if further research confirms, these findings may serve valuably in precisely selecting the best-suited treatment options for such patient.

Footnotes

Author Contributions

E.T., U.S., D.O., S.S, and N.K.D. conceived the study, participated in the study’s design, and performed clinical examination and statistical analysis. All authors contributed significantly and equal, and all authors approved the final form of the manuscript.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

Ethical Statement

Data Availability Statement

The present data belongs to and is stored at the Baskent University Faculty of Medicine; it cannot be shared without permission. Researchers who meet the criteria for access to confidential data should contact the Baskent University Department of Radiation Oncology at adanabaskent@baskent.edu.tr.

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Prognostic Value of Pre-Chemoradiotherapy Pan-Immune-Inflammation Value (PIV) in Locally Advanced Nasopharyngeal Cancers

Abstract

Background

Methods

Results

Conclusions

Keywords

Introduction

Patients and Methods

Study Design and Participants

Ethics and Consent

Chemoradiotherapy Protocol

Measurement of Pan-Immune-Inflammation Value

Response Assessment

Clinical Endpoints and Statistics

Results

Discussion

Conclusion

Footnotes

Author Contributions

Declaration of Conflicting Interests

Funding

Ethical Statement

Data Availability Statement

References