Numerical Chromosomal Aberration in NodePositive Early Gastric Carcinomas: Analysis by Fluorescence in Situ Hybridization and Immunohistochemical Staining
Restricted accessResearch articleFirst published online October, 1998
Numerical Chromosomal Aberration in NodePositive Early Gastric Carcinomas: Analysis by Fluorescence in Situ Hybridization and Immunohistochemical Staining
Tissue from surgically resected early gastric carcinomas with nodal involvement (NI) and without nodal involvement (NNI) were examined by fluorescence in situ hybridization (FISH) by use of a set of alpha-satellite probes. Numerical gain was observed in seven cases (70%) by a probe of chromosome 17 and in three cases (30%) by a probe of chromosome 18 among the NI cases, whereas such a gain was not detected in any NNI cases examined. Cases with numerical aberration in chromosome 17 exhibited immunohistochemical positivity of oncogene or tumor suppressor gene products related to chromosome 17 more frequently than the cases lacking those aberrations. Numerical chromosomal aberration as well as DNA aneuploidy determined by FISH may be related to genetic instability and biological behavior in early gastric carcinomas.
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