Restricted accessResearch articleFirst published online 1999-01
Dedifferentiated Components Versus Well-differentiated Components in Dedifferentiated Lip sarcoma: A Comparative Study of Their Proliferative Activity and Interphase Cytogenetics Using MIB-1 Immunostaining and Fluorescence in Situ Hybridization
We retrospectively analyzed the proliferative activity and the centromeric copy number of chromosomes 8, 12, and 17 in both well-differentiated components and dedifferentiated ones of 10 dedifferentiated lip sarcoma cases by using MIB-1 immunostaining and fluorescence in situ hybridization (FISH) on the paraffin-embedded tissue specimens. The MIB1 labeling index (LI) in dedifferentiated components was higher than that in the well-differentiated components (p<.01). Chromosomal aberrations such as gains in chromosomes 8, 12, and 17 were found in two dedifferentiated components of the seven cases in which signals were detected. The most frequent aberrations were a gain in chromosome 17 (cases 2 and 10), followed by gains in chromosomes 8 (case 10) and 12 (case 2). In contrast, no chromosomal aberrations were observed in any of the eight well-differentiated components in which signals were detected. Based on these findings, chromosomal aberrations in the dedifferentiated components may reflect aggresive tumor progression in dedifferentiated lip sarcoma.
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