Critical Comparison of 31 Commercially Available Digital Slide Systems in Pathology

Abstract

Advances in new technologies for complete slide digitization in pathology have allowed the appearance of a wide spectrum of technologic solutions for whole-slide scanning, which have been classified into motorized microscopes and scanners. This article describes technical aspects of 31 different digital microscopy systems. The most relevant characteristics of the scanning devices are described, including the cameras used, the speed of digitization, and the image quality. Other aspects, such as the file format, the compression techniques, and the solutions for visualization of digital slides, (including diagnosis-aided tools) are also considered. Most of the systems evaluated allow a high-resolution digitization of the whole slide within about 1 hour using a ×40 objective. The image quality of the current virtual microscopy systems is suitable for clinical, educational, and research purposes. The efficient use of digital microscopy by means of image analysis systems can offer important benefits to pathology departments.

Keywords

virtual microscopy whole slide scanning motorized microscope

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References

Ferreira R , Moon B , Humphries J , et al. The virtual microscope. Proc AMIA Annu Fall Symp. 1997;449-453. Available from: http://citeseer.ist.psu.edu/ferreira97virtual.html. Accessed June 8, 2006.

Afework A , Beynon MD , Bustamante F , et al. Digital dynamic telepathology—the virtual microscope. Proc AMIA Symp. 1998;912-916. Available from: http://citeseer.ist.psu.edu/afework98digital.html. Accessed June 8, 2006.

The EORTC Tumor Bank. European Organisation for Research and Treatment of Cancer (EORTC). Available from: http://www.eortc.be/vtb/tumorbank/. Accessed June 8, 2006.

Costello SP , Johnston DJ , Dervan PA , O’Shea DG. Development and evaluation of the virtual pathology slide: a new tool in telepathology. J Med Internet Res. 2003;5: e11-e11. Available from: http://www.jmir.org/2003/2/e11/. Accessed June 8, 2006.

Galvez J , Munn RJ , Garbutt AW , Cardiff RD. The development of the mouse models of human cancer consortium image archives. Advancing pathology informatics, imaging, and the Internet (APIII 2002). Scientific Session and E-Poster Session Abstracts. Arch Pathol Lab Med. 2003;127: 806-806. Available from: http://arpa.allenpress.com/arpaonline/?request=get-toc&issn=1543-2165&volume=127&issue=7. Accessed June 8, 2006.

García Rojo M , Bueno García G , González García J , Carbajo Vicente M. Preparaciones digitales en los servicios de Anatomía Patolorgica (I). Aspectos básicos de imagen digital. Rev Esp Patol. 2005;38: 69-77. Available from: http://www.pgmacline.es/revpatologia/volumen38/vol38-num2/38-2n02.htm. Accessed June 8, 2006.

Patel AA , Gil-Bertson J , Dhir R , Becich MJ. Virtual quality assurance protocol: a quality assurance protocol for pathology slides from multiple institutes. advancing pathology informatics, imaging, and the Internet (APIII 2002). Scientific Session and E-Poster Session Abstracts. Arch Pathol Lab Med. 2003;127: 804-805. Available from: http://arpa.allenpress.com/arpaonline/?request=get-toc&issn=1543-2165&volume=127&issue=7. Accessed June 8, 2006.

Olson AH , Eichhorn O , Saligrama K. Algorithmic processing of high resolution virtual slides. Advancing practice, instruction, and innovation through informatics (APIII 2004): Scientific Session and E-Poster Abstracts. Arch Pathol Lab Med. 2005;129: 819-820. Available from: http://arpa.allenpress.com/arpaonline/?request=get-toc&issn=1543-2165&volume=129&issue=6. Accessed June 8, 2006.

Weinstein RS , Descour MR , Liang C , et al. An array microscope for ultrarapid virtual slide processing and telepathology. Design, fabrication, and validation study. Hum Pathol. 2004;35: 1303-1314. Available from: http://dx.doi.org/10.1016/j.humpath.2004.09.002. Accessed June 8, 2006.

10.

Weinstein RS. Innovations in medical imaging and virtual microscopy. Hum Pathol. 2005;36: 317-319.

11.

Hitchcock CL , Hitchcock LE. Three years of experience with routine use of telepathology in assessment of excisional and aspirate biopsies of breast lesions. Croat Med J. 2005;46: 449-457. Available from: http://www.cmj.hr/2005/46/3/15861526.pdf. Accessed June 8, 2006.

12.

Habib I. Automated microscope slide analysis in pathology. Los Angeles, CA: IVD Technology; 2005. Available from: http://www.devicelink.com/ivdt/archive/05/05/001.html. Accessed June 6, 2006.

13.

Divi RL , Dragan YP , Pitot HC , Poirier MC. Immunohistochemical localization and semi-quantitation of hepatic tamoxifen-DNA adducts in rats exposed orally to tamoxifen. Carcinogenesis. 2001;22: 1693-1699. Available from: http://carcin.oxfordjournals.org/cgi/content/full/22/10/1693. Accessed June 8, 2006.

14.

Gutman SI. Warning letter. Rockville, MD: Food and Drug Administration; 2003. Available from: http://www.fda.gov/foi/warning_letters/g3970d.htm. Accessed June 8, 2006.

15.

Gonzalez J. Digital microscopy applications in the Virtual Pathology Congress (http://www.conganat.org). SEAP regional meeting. Ciudad Real: Sociedad Española de Anatomía Patológica; 2006. Available from: http://www.seap.es/regional/clm/. Accessed June 8, 2006

16.

Gilbertson JR , Ho J , Anthony L , Jukic DM , Yagi Y , Parwani AV. Primary histologic diagnosis using automated whole slide imaging: a validation study. BMC Clin Pathol. 2006;6: 4-4. Available from: http://www.biomedcentral.com/1472-6890/6/4. Accessed June 8, 2006.