Purpose: This study investigates the potential effectiveness of an end-of-life care volunteer online self-learning platform and factors influencing volunteers’ satisfaction. Method: Participants completed an online questionnaire before and after the training modules; 173 of the 274 initial respondents completed the post-training assessment. We used a paired samples t-test to assess the potential effectiveness of the online learning intervention and employed partial least squares structural equation modelling to examine associations between the predictors and online learning satisfaction. Results: Findings revealed a significant improvement in learning scores post-training. Key factors associated with online learning satisfaction included perceived usefulness, course quality, and learning flexibility. The study underscores the importance of tailored content and flexible schedules in enhancing end-of-life care volunteer training. Discussion: The findings validate the Technology Acceptance Model integrated with the User Satisfaction Model, highlighting the critical role of perceived usefulness, course quality, and flexibility in achieving online learning satisfaction.