Lipopolysaccharide-induced proliferation and adhesion of U937 cells to endothelial cells involves barium chloride sensitive hyperpolarization

Abstract

The adhesion of monocytes to the endothelium and their proliferation in the subendothelial space play an important role in atherosclerosis. Since the proliferation and migration of cells are influenced by the activity of ion channels, the aim of this study was to examine whether barium chloride (Ba²⁺)-sensitive potassium channels (K_iCa) are involved in lipopolysaccharide (LPS)-induced proliferation of monocytic U937 cells, and in the adhesion of these cells to endothelial cells. The adhesion of LPS-stimulated U937 cells to endothelial cells reached a maximum at a concentration of 5 µg/ml. This effect of LPS was completely abolished in the presence of Ba²⁺ (100 µmol/l). In addition, LPS-induced proliferation was significantly reduced by Ba ²⁺ (control, 100%; LPS 5 µg/ml, 175%; LPS + Ba²⁺ 100 µmol/l, 136%; n = 12, P < 0.05). To examine whether K_iCa are activated by LPS, changes of U937 membrane potential were determined. LPS (5 µg/ml) caused a hyperpolarization of U937 cells indicating a flux of K⁺ ions out of the cells. This effect was completely blocked by Ba²⁺ (100 µmol/l). In conclusion, we demonstrate that LPS activates K_iCa in U937 cells, which is responsible for LPS-induced adhesion of these cells to endothelial cells, and to the proliferation of U937 cells.