Abstract
This study aimed to investigate the interaction between fibroblastic cells and poly-L-lactide (PLLA) composite film containing bovine bone. The effects of PLLA/bovine bone on cell cytotoxicity, attachment, proliferation and differentiation of fibroblasts in-vitro were investigated. The results of cytotoxicity tests showed that cells cultured in extracts of PLLA/bovine bone and on the surface of composites showed normal growth and proliferation, and mineralization nodules were observed for fibroblasts cultured in PLLA/bovine bone extract at day 7 by using von Kossa staining and tetracycline staining. Compared with pure PLLA materials, bovine bone in the PLLA composites facilitated both adhesion and proliferation of rat fibroblasts on the PLLA/bovine bone composite film. In conclusion, these results suggest that the introduction of bovine bone may improve the cell response involved in the osteogenesis and thus may be beneficial to further modify poly-L-lactide as a better bone repairing material.