Sage Journals: Discover world-class research

Abstract

In the immunocompromised setting, recipients of solid-organ or hematopoietic stem-cell transplants carry an increased risk of post-transplant lymphoproliferative disorder (PTLD). Burkitt lymphoma (BL) PTLD is a rare form of monomorphic B-cell PTLD, which lacks a standard best treatment. Here, we report the successful treatment of refractory BL-PTLD with autologous anti-CD19 chimeric antigen receptor (CAR) T-cell therapy. A male patient was diagnosed with BL-PTLD, with an increasing Epstein-Barr virus (EBV) viral load, at 21 months after undergoing living liver transplantation from his mother due to neonatal biliary atresia. After 10 cycles rituximab +/− intensive chemotherapy and surgical tumor resection, the tumors significantly advanced. Next-generation sequencing (NGS) was performed on formalin-fixed paraffin-embedded tumor tissue, revealing one mutation in exon 5, TP53: p.A159 V, which may be associated with chemo-resistance. Thus, treatment was started with autologous anti-CD19 CAR T-cell therapy. We administered 9.0 × 10⁶/kg autologous anti-CD19 CAR T-cells, after conditioning with cyclophosphamide and fludarabine. Unexpectedly, the patient experienced only mild (Grade II) cytokine release syndrome (CRS) without neurotoxicity. Finally, he went into complete remission (CR), and has achieved 16-month event-free survival to date. In addition, liver function has remained stably within the normal range without any immunosuppressive therapy. The literature includes only five previously reported BL cases treated with CAR T-cell therapy. In conclusion, the present case suggests that autologous anti-CD19 CAR T-cell therapy may represent a new therapeutic option for some cases of refractory BL-PTLD.

Clinical trial number: ChiCTR2000032211.

Keywords

PTLD Burkitt lymphoma CAR T-cells CD19

Introduction

Post-transplantation lymphoproliferative disorder (PTLD) is a rare but severe complication of solid-organ or allogeneic hematopoietic stem-cell transplantation, which is related to immunosuppression and the Epstein-Barr virus (EBV)¹. The PTLD incidence after liver transplantation ranges from 1.0–5.5% in the adult population. The majority of cases are diagnosed in the first year post-transplant, although this disorder may occur at any time². Burkitt lymphoma (BL) PTLD is a rare form of monomorphic B-cell PTLD that is associated with poor outcomes³. The first-line treatment involves rituximab-based chemotherapy, but other therapeutic strategies for BL-PTLD include reduction of immunosuppression⁴, surgical resection of localized disease, local radiation, immunochemotherapy, and cellular immunotherapy, such as with EBV-specific cytotoxic T-lymphocytes (CTLs)⁵. There are no reported clinical trials for BL-PTLD treatment, and the lack of standard treatment modalities renders BL-PTLD management challenging.

Here, we report the first case of refractory BL-PTLD that was successfully treated using autologous anti-CD19 chimeric antigen receptor (CAR) T-cell therapy, which yielded sustained complete remission (CR) and manageable cytokine release syndrome (CRS). We also review the relevant medical literature.

Materials and Methods

Diagnosis and Clinical History of a Child with BL-PTLD

The male patient was born with pathological jaundice, and was diagnosed with neonatal biliary atresia. He underwent Kasai portoenterostomy, a palliative bilio-intestinal derivation procedure, but still developed decompensated cholestatic cirrhosis. At the age of 13 months, he received living liver transplantation (LT) using a lateral left-segment graft from his mother. Post-transplantation immunosuppression was performed using tacrolimus and prednisone.

At four months after liver transplantation, the patient showed positive EBV status. The virus DNA load gradually increased, reaching 3.9 × 10⁴ copies/ml at 10 months after liver transplantation. At this time, the tacrolimus dose was halved and kept at below 5 ng/ml, but the EBV DNA load remained at a high level (Fig. 1A).

Figure 1.

Patient’s clinical features before chimeric antigen receptor (CAR) T-cell therapy. (A) The trend of EBV DNA load before CAR T-cell therapy. Red arrows indicate diagnosis of Burkitt lymphoma post-transplant lymphoproliferative disorder (BL-PTLD). (B) Upon diagnosis, positron emission tomography-computed tomography (PET-CT) revealed multiple nodules in the abdomen and pelvis, multiple enlarged lymph nodes in the left axilla and both sides of the neck, and multiple skeletal lesions exhibiting hypermetabolism. (C) After four rounds of chemotherapy, PET-CT showed that the tumor had increased in size. (D) At 55 days after CAR T-cell infusion, PET-CT revealed no typical malignant tumor-like hypermetabolic lesions anywhere in the patient’s body. (E) Next-generation sequencing (NGS) was performed on a tumor tissue samples, revealing one mutation in exon 5, TP53: p.A159 V, which may be associated with chemo-resistance. (F) The specific treatment process used in this case. The red five-pointed star indicates surgical tumor resection, and the red triangles indicate radiotherapy (total 25 Gy) and tomotherapy (total 16 Gy).

At 21 months after liver transplantation, the boy presented with lymphadenopathy in the neck, an abdominal mass of ∼7 × 10 cm in size, and repeated abdominal pain and vomiting. Positron emission tomography-computed tomography (PET-CT) revealed multiple nodules in the abdomen and pelvis, multiple enlarged lymph nodes in the left axilla and both sides of the neck, and multiple skeletal lesions that exhibited hypermetabolism (Fig. 1B). Abdominal magnetic resonance imaging (MRI) further revealed multiple abnormal signals in both kidneys, with hydronephrosis in the right kidney. The EBV viral load in peripheral blood was 5.51 × 10⁵ copies.

Lymph-node mass resection was performed on the left side of his neck. Pathological morphology revealed tumor cells that were diffusely spread out and densely proliferated, exhibiting medium cell volume, small cytoplasm, and large nuclei, along with obvious apoptosis and starry-sky appearance. Immunohistochemical analysis indicated that the tumor cells were CD19⁺, CD20⁺, CD10⁺, Bcl6⁺, Bcl-2 part⁺, Ki67⁺ (98%), CD3⁻, CD30⁻, and ALK⁻ (Fig. 2A, B). EBV status was positive based on in situ hybridization for EBV-encoded small RNA (EBER) in resected tissue (Fig. 2C). Fluorescence in situ hybridization (FISH) indicated an abnormal C-myc gene fraction (Fig. 2D). Based on these findings, the diagnosis was BL-PTLD according to the 2008 World Health Organization (WHO) classification⁶.

Figure 2.

Pathological analysis of the lymph-node mass led to diagnosis of Burkitt lymphoma post-transplant lymphoproliferative disorder (BL-PTLD). (a) Pathological morphology (HE staining; 10 × 20) showed tumor cells diffusely spread out and densely proliferated, exhibiting medium cell volume, small cytoplasm, and large nuclei, with a quasi-circular shape. Obvious apoptosis and starry-sky appearance were observed. (b) Immunohistochemistry (10 × 20) indicated strong uniform membrane expression of CD19 on tumor cells. (c) EBV-encoded small RNA (EBER) in situ hybridization (10 × 20) revealed strong uniform nuclear expression of EBER in tumor cells. (d) C-myc gene fluorescence in situ hybridization (FISH; 10 × 100). Red arrows indicate abnormal C-myc gene fracture.

Tacrolimus was stopped upon the diagnosis of PTLD. The patient was administered two courses of rituximab, cyclophosphamide, methylprednisone, and intrathecal prophylaxis (I/T) with methotrexate, dexamethasone, and cytarabine; followed by two courses of cyclophosphamide AND methylprednisone. However, subsequent PET-CT revealed that the tumor had increased in size (Fig. 1C). Thus, four more intensive rituximab-based regimens were added. At this time, the EBV viral load was negative. Next, the boy underwent ileocecal tumor resection and lymph node dissection to reduce the tumor burden. Pathological examination indicated continued existence of tumor cells with necrosis. Surgery was followed by two more chemotherapy courses. Subsequent computed tomography (CT) revealed multiple soft tissue masses in the abdominal cavity, which were significantly more advanced than those previously observed. Next-generation sequencing (NGS) was performed on a formalin-fixed paraffin-embedded tissue sample, revealing one mutation in exon 5, TP53: A159 V (Fig. 1E), which may be associated with chemo-resistance. In an effort to reduce the tumor burden, the patient was administered radiotherapy (total 25 Gy) and tomotherapy (total 16 Gy). Figure 1F presents the specific treatment process.

Results

Administration of Autologous Anti-CD19 CAR T-Cells

Since the patient suffered chemo-resistance disease, we considered administering salvage treatment with CAR T-cells. However, data regarding the efficacy of CAR T-cell therapy in BL are lacking (Table 1)^7,8. In our experiences of treating over 100 cases of refractory and resistant CD19-positive B-cell acute lymphoblastic leukemia (ALL), CR has been achieved in more than 98% of patients (data not reported). Thus, we decided to administer CAR T-cell therapy. The patient was enrolled in our trial (ChiCTR2000032211) and informed consent was signed by the patient’s guardian. Peripheral blood CD3-positive T lymphocytes were collected from the boy. These T-cells were activated for two days, and then transduced with murinized second-generation FMC63-based anti-CD19 CAR lentivirus, with signals provided by the costimulatory molecules 4-1BB and CD3-zeta. Five days later, a sufficient number of cells were washed and resuspended in saline solution with 2.5% human serum albumin (Fig. 3A). The transfection rate of CAR T-cells was 49.1% (Fig. 3B). To confirm the cytotoxic effect of the CAR T-cells, we incubated anti-CD19 CAR T-cells from the patient, CAR T-cells from a healthy donor, or untransduced T-cells with ALL cell lines (GFP-positive Reh cells) for 24 h at an effector-to-target ratio of 1:4. Both kinds of CAR T-cells killed almost all Reh cells, indicating that the patient’s CAR T-cells had cytotoxic effects comparable with CAR T-cells from a healthy donor (Fig. 3C).

Table 1.

Treatment and Outcomes from Prior Trials of CAR T-Cell Therapy for Burkitt Lymphoma (Literature Review).

Trial reference	Number of patients	Age (years)	Target	Cell number (×10⁶)	CRS	HSCT	Outcome
Avigdor et al., 2018	1	32	CD19	1	II	Yes	CR
Cao et al., 2019	1	26	CD19	11	II	No	PR
Li et al., 2019	2	Adults	CD19	5–10	Unclear	No	Unclear
Du and Zhang, 2020^a	1	8	CD19	2	I	No	CR
			CD22	2.11	I
			CD20	1	III

^a This case was treated with sequential anti-CD19, 22, and 20 autologous CAR T-cell therapies.

HSCT: hematopoietic stem cell transplantation.

Figure 3.

Chimeric antigen receptor (CAR) T-cell therapy efficiency and clinical response. (A) Schematic of CAR T-cell treatment. (B) Flow cytometric analysis of CAR T-cell expression. Transduced live singlet cells were stained with primary anti biotin–F(ab)′2, followed by streptavidin-PE (light blue). Nontransduced cells (pink) served as negative controls. (C) Anti-CD19 CAR T-cells from the patient, CAR T-cells from a healthy donor, or untransduced T-cells were incubated with GFP-overexpressing ALL cell lines (GFP⁺ Reh) for 24 h at an effector-to-target ratio of 1:4. Cytotoxic effects were measured by flow cytometry. (D) Trends of changes in maximum body temperature, and white blood cell (WBC) and lymphocyte counts after CAR T-cell treatment. (E) Trends of changes in C-reactive protein (CRP), interleukin-6 (IL-6), and serum lactate dehydrogenase (LDH) after CAR T-cell treatment. (F) Changes in the approximate tumor volume according to B ultrasound.

Starting on day 5, the patient was administered conditioning chemotherapy (cyclophosphamide 500 mg/m²/day and fludarabine 40 mg/m²/day) for two days to deplete lymphocytes. Next, on day 0, the child was infused with fresh anti-CD19 CAR T-cells at a total dose of 9.0 × 10⁶ /kg. He was closely monitored for clinical symptoms and physical signs, and grade II CRS was observed without neurotoxicity according to the ASTCT criteria⁹. On days 2–5, the patient experienced a high fever (Fig. 3D). We also detected a significant increase of serum inflammatory cytokines, especially interleukin-6 (IL-6), with a peak IL-6 level of 12245.58 pg/ml on day 4 (Fig. 3E). The trends of changes in C-reactive protein (CRP), lactate dehydrogenase (LDH), and body temperature were consistent with the cytokine levels and the patient’s clinical symptoms (Fig. 3D, E). To control CRS, the patient was administered the anti-interleukin-6 receptor antibody tocilizumab 160 mg/day for 3 days (days 2–4). We did not use glucocorticoid due to concerns about impairing the expansion and antitumor activity of the CAR T-cells. On day 6, the child’s body temperature returned to normal. A series of routine blood panel examinations revealed that the white blood cells and lymphocytes were lower in peripheral blood after lymphodepleting treatment, and then increased on 7–11 days after CAR T-cells infusion (Fig. 3D). The patient then experienced vomiting for six days but recovered after supporting therapy. The patient showed no cough, headache, hypotension, or seizure during treatment, and all physical signs of CRS were manageable.

Exceptional Tumor Response to CAR T-Cells and Stable Graft Function

After CAR T-cell therapy, the tumor size was monitored weekly by abdominal and pelvic B ultrasound. The approximate volume was gradually reduced (Fig. 3F). On day 55, a PET-CT scan was performed, which revealed that the previously observed abdominal, pelvis, and multiple skeletal lesions had essentially disappeared. At this time, no typical malignant tumor-like hypermetabolic lesions were observed anywhere in his whole body. Thus, these imaging results indicated that the patient had achieved CR (Fig. 3D).

Abdominal CT scanning has been repeated every 6 months since then, with no typical tumor lesions discovered. Disease-free survival has persisted for 16 months to date. Other tests have been normal, with the exception of B-cell aplasia, requiring immunoglobulin replacement (0.5g/kg per month) to maintain immunoglobulin levels. The boy has remained free from any serious infection.

Graft function has been monitored monthly by blood test. Until now, the patient does not receive any immunosuppressive therapy, and the liver function has remained stably within the normal range. A liver biopsy was performed 12 months after CAR T-cell therapy, which revealed mild lymphocyte infiltration and hepatic lobule fibrosis, with no sign of rejection. The chimerism assay based on the rs4646006(del) and rs1610937(insert) sites of the donor revealed a 0.005% chimeric rate, which may partially account for the immunotolerance.

Discussion

Current knowledge of PTLD is limited due to its rarity, morphologic heterogeneity, and the lack of prospective trials. Recent data have established rituximab as the standard of treatment, either as monotherapy or followed by systemic chemotherapy^10,11. A less-aggressive low-dose chemotherapy regimen comprising cyclophosphamide and prednisolone (Cy/Pred) with rituximab yielded a 100% overall response rate (five CR and one PR) for PTLD treatment¹². However, some BL-PTLD patients still experience disease progression or other treatment failures despite these improvements, and these patients face a dismal prognosis¹³. There remains a need for new and tolerable therapies for such cases. Some researchers explored autologous or third-party EBV-specific CTL to treat rituximab-refractory PTLDs, and the response rates were about 50%∼60%¹⁴. However, considering the partial human leukocyte antigen (HLA) matching and allogeneic nature of the third-party CTL, we expected them to be short-lived in vivo since the host immune system would target the mismatched HLA molecules. Besides, none of the EBV-specific T-cells products have yet qualified in China. Chemo-resistance is driven by TP53 mutations in many different cancers, including aggressive B-cell lymphomas. In the presently described case, based on the failure of rituximab-based intensive chemotherapy and the finding of TP53 p.A159 V mutation in tumor tissues, we decided to administer autologous anti-CD19 CAR T-cell therapy.

To our knowledge, currently, no trials investigating the use of CAR T-cells for BL-PTLD treatment, many trials have proven the effectiveness of CAR T-cells in B-cell lymphomas¹⁵. Thus, one could hypothesize that similar results might be obtained in the setting of BL-PTLD. Although there was one trial that reported poor response and death outcomes for three patients with diffuse large B cell lymphomas (DLBCL) PTLD after heart and kidney transplant received CAR T-cell therapy¹⁶. In one case of an adult patient with refractory BL, a complete response was achieved with anti-CD19 CAR T-cells¹⁷. Here, we report the first case of refractory BL-PTLD that was successfully treated using autologous anti-CD19 CAR T-cells, which yielded sustained complete remission with an acceptable toxicity profile.

When CAR T-cells engage with target cells, they release a variety of cytokines and chemokines. Macrophages and other innate immune cells also become activated and contribute to this cytokine production¹⁸. Thus, CRS is the most frequent adverse reaction, which often occurs within two weeks of treatment. Fever is the earliest and most common CRS symptom. Tocilizumab blocks the IL-6 receptor (IL-6 R) and is considered as the first-line treatment for severe CRS. Glucocorticoids exert a wide range of immunosuppressive effects and are commonly used as second-line immunosuppressive drugs. Multivariable analysis of baseline characteristics has identified high tumor burden as an independent predictor of severe CRS¹⁹. Unexpectedly, our patient experienced only mild CRS.

In our patient, B-cell aplasia occurred as an on-target but off-tumor toxicity, which necessitated immunoglobulin replacement to maintain normal immunoglobulin levels. A prior study reported that B-cell aplasia occurred in all patients who exhibited a response to CAR T-cell therapy and could persist for up to one year after CAR T-cells were no longer detectable by flow cytometry²⁰. Notably, B-cell aplasia can be used as a pharmacodynamic measure of the persistence of functional anti-CD19 CAR T-cells²¹.

Resistance or relapse has been reported in patients receiving CD19-directed CAR T-cell therapy. At Boren Hospital in Beijing, China, a child with relapsed refractory BL was administered sequential treatments with anti-CD19, 22, and 20 CAR T-cells. Unfortunately, the patient showed no discernible response and exhibited progressive disease following anti-CD19 CAR T-cell treatment²². BL relapse reportedly occurs mostly within the first six months of treatment²³. The boy in our present case has survived without relapse for 16 months to date. There remains a need for additional clinical data from cases of relapsed/refractory BL-PTLD to determine whether it is preferable to develop CAR T-cells against multiple targets or against CD19 alone and whether CAR T-cell therapy should optimally be used as a solo curative therapy.

Immune system balance is the key for PTLD treatment. Calcineurin inhibitors (tacrolimus and cyclosporin) are the mainstay of liver transplantation immunosuppression, but result in EBV amplification, as well as side effects, such as nephrotoxicity and hypertension²⁴. Reduction or withdrawal of immunosuppression is associated with decreases of PTLD incidence and PTLD-related mortality. However, insufficient immunosuppression can lead to high rejection rates²⁵. Immunosuppressive therapy should routinely be withdrawn during PTLD treatment; however, the timing of restarting immunosuppressive therapy requires careful monitoring of graft function and immune status. In our present case, the graft has maintained a stable condition without any immunosuppressive therapy, as demonstrated by essentially normal serum biochemistry results. This immunotolerance is encouraging since it confirmed the high specificity of the CAR T-cells, and the achievement of immune system balance. The existence of chimeric cells in the boy at one year after liver transplantation suggests the formation of long-term chimerism, which may partially explain the tolerance²⁶. However, a biopsy of the graft suggested mild immunoreaction, which could lead to chronic injury. This finding alerted us to the necessity of restarting immunosuppressive medication and performing further biopsies in future follow-up, even though liver function assay results are normal.

Conclusions

Here we reported the first case of chemotherapy-resistant BL-PTLD in which anti-CD19 CAR T-cell treatment achieved continuous complete remission. These results indicate that CAR T-cell therapies could represent a new safe and effective therapeutic option for some cases of PTLD, as long as close monitoring is provided and adverse reactions are quickly treated. Additional clinical trial data are needed to further validate the long-term safety and efficacy of anti-CD19 CAR T-cell therapy for BL-PTLD.

Footnotes

Acknowledgments

We thank the patient and his parents for agreeing the paper published.

Author Contributions

Tianyi Wang and Mingxuan Feng drafted the manuscript. Chengjuan Luo, Xinyu Wan, Ci Pan, Jingyan Tang, Feng Xue, Minzhi Yin, Dongqing Lu participated in the writing of the paper and data analysis. Qiang Xia, Benshang Li, Jing Chen participated in research design and revised the manuscript multiple times. All authors have read and approved the final manuscript.

Ethical Approval

This study was approved by the Ethics Committee of Shanghai Children’s Medical Center, Shanghai, China (SCMCIRB-K2016067 -1).

Statement of Human and Animal Rights

All procedures in this study were conducted in accordance with the Ethics Committee of Shanghai Children’s Medical Center, Shanghai, China (SCMCIRB-K2016067 -1) approved protocols. This study was conducted in accordance with the Helsinki Declaration for human studies.

Statement of Informed Consent

Written informed consent was obtained from the patient’s guardians for their anonymized information to be published in this article.

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This study was supported by Shanghai Collaborative Innovation Center for Translational Medicine (TM201928), and the National Natural Science Foundation of China (81670174, 81670136).

ORCID iD

Jing Chen

References

Engels

Pfeiffer

Fraumeni

Jr Kasiske

Israni

Snyder

Wolfe

Goodrich

Bayakly

Clarke

Copeland

. et al. Spectrum of cancer risk among US solid organ transplant recipients. JAMA. 2011;306(17):1891–1901.

Opelz

Dohler

. Lymphomas after solid organ transplantation: a collaborative transplant study report. Am J Transplant. 2004;4(2):222–230.

Zimmermann

Reinke

Neuhaus

Lehmkuhl

Oertel

Atta

Planker

Gartner

Lenze

Anagnostopoulos

Riess

. et al. Burkitt post-transplantation lymphoma in adult solid organ transplant recipients: sequential immunochemotherapy with rituximab (R) followed by cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP) or R-CHOP is safe and effective in an analysis of 8 patients. Cancer. 2012;118(19):4715–4724.

Tsai

Hardy

Tomaszewski

Kotloff

Oltoff

Somer

Schuster

Porter

Montone

Stadtmauer

. Reduction in immunosuppression as initial therapy for posttransplant lymphoproliferative disorder: analysis of prognostic variables and long-term follow-up of 42 adult patients. Transplantation. 2001;71(8):1076–1088.

Haque

Wilkie

Jones

Higgins

Urquhart

Wingate

Burns

McAulay

Turner

Bellamy

Amlot

. et al. Allogeneic cytotoxic T-cell therapy for EBV-positive posttransplantation lymphoproliferative disease: results of a phase 2 multicenter clinical trial. Blood. 2007;110(4):1123–1131.

Campo

Swerdlow

Harris

Pileri

Stein

Jaffe

. The 2008 WHO classification of lymphoid neoplasms and beyond: evolving concepts and practical applications. Blood. 2011;117(19):5019–5032.

Cao

Sun

Jin

Cheng

Wang

Yuan

Lyu

Zhao

. Anti-CD19 chimeric antigen receptor T cells in combination with nivolumab are safe and effective against relapsed/refractory B-cell non-hodgkin lymphoma. Front Oncol. 2019;9:767.

Zhang

Chen

Lou

Chen

Kang

Tan

Sun

. et al. Comparation of CART19 and autologous stem-cell transplantation for refractory/relapsed non-Hodgkin’s lymphoma. JCI Insight. 2019;5(17):e130195.

Lee

Santomasso

Locke

Ghobadi

Turtle

Brudno

Maus

Park

Mead

Pavletic

. et al. ASTCT consensus grading for cytokine release syndrome and neurologic toxicity associated with immune effector cells. Biol Blood Marrow Transplant. 2019;25(4):625–638.

10.

Choquet

Leblond

Herbrecht

Socie

Stoppa

Vandenberghe

Fischer

Morschhauser

Salles

Feremans

Vilmer

. et al. Efficacy and safety of rituximab in B-cell post-transplantation lymphoproliferative disorders: results of a prospective multicenter phase 2 study. Blood. 2006;107(8):3053–3057.

11.

Evens

David

Helenowski

Nelson

Kaufman

Kircher

Gimelfarb

Hattersley

Mauro

Jovanovic

Chadburn

. et al. Multicenter analysis of 80 solid organ transplantation recipients with post-transplantation lymphoproliferative disease: outcomes and prognostic factors in the modern era. J Clin Oncol. 2010;28(6):1038–1046.

12.

Orjuela

Gross

Cheung

Alobeid

Morris

Cairo

. A pilot study of chemoimmunotherapy (cyclophosphamide, prednisone, and rituximab) in patients with post-transplant lymphoproliferative disorder following solid organ transplantation. Clin Cancer Res. 2003;9(10 Pt 2):3945S–3952S.

13.

Cairo

Sposto

Perkins

Meadows

Hoover-Regan

Anderson

Siegel

Lones

Tedeschi-Blok

Kadin

Kjeldsberg

. et al. Burkitt’s and Burkitt-like lymphoma in children and adolescents: a review of the Children’s Cancer Group experience. Br J Haematol. 2003;120(4):660–670.

14.

Prockop

Doubrovina

Suser

Heller

Barker

Dahi

Perales

Papadopoulos

Sauter

Castro-Malaspina

Boulad

. et al. Off-the-shelf EBV-specific T cell immunotherapy for rituximab-refractory EBV-associated lymphoma following transplantation. J Clin Invest. 2020;130(2):733–747.

15.

Shah

Maatman

Hari

Johnson

. Multi targeted CAR-T cell therapies for B-cell malignancies. Front Oncol. 2019;9:146.

16.

Krishnamoorthy

Ghobadi

Delos Santos

Schilling

Malone

Murad

Bartlett

Alhamad

. CAR-T therapy in solid organ transplant recipients with treatment refractory post transplant lymphoproliferative disorder. Am J Transplant. 2020.

17.

Avigdor

Shouval

Jacoby

Davidson

Shimoni

Besser

Nagler

. CAR T cells induce a complete response in refractory Burkitt Lymphoma. Bone Marrow Transplant 2018;53(12):1583–1585.

18.

Norelli

Camisa

Barbiera

Falcone

Purevdorj

Genua

Sanvito

Ponzoni

Doglioni

Cristofori

Traversari

. et al. Monocyte-derived IL-1 and IL-6 are differentially required for cytokine-release syndrome and neurotoxicity due to CAR T cells. Nat Med. 2018;24(6):739–748.

19.

Hay

Hanafi

Gust

Liles

Wurfel

Lopez

Chen

Chung

Harju-Baker

Cherian

. et al. Kinetics and biomarkers of severe cytokine release syndrome after CD19 chimeric antigen receptor-modified T-cell therapy. Blood. 2017;130(21):2295–2306.

20.

Kalos

Levine

Porter

Katz

Grupp

Bagg

June

. T cells with chimeric antigen receptors have potent antitumor effects and can establish memory in patients with advanced leukemia. Sci Transl Med. 2011;3(95):95ra73.

21.

Maude

Frey

Shaw

Aplenc

Barrett

Bunin

Chew

Gonzalez

Zheng

Lacey

Mahnke

. et al. Chimeric antigen receptor T cells for sustained remissions in leukemia. N Engl J Med. 2014;371(16):1507–1517.

22.

Zhang

. Sequential anti-CD19, 22, and 20 autologous chimeric antigen receptor T-cell (CAR-T) treatments of a child with relapsed refractory Burkitt lymphoma: a case report and literature review. J Cancer Res Clin Oncol. 2020;146(6):1575–1582.

23.

Rigaud

Auperin

Jourdain

Haouy

Couec

Aladjidi

Gandemer

Lambliotte

Plat

Landman-Parker

Michon

. et al. Outcome of relapse in children and adolescents with B-cell non-Hodgkin lymphoma and mature acute leukemia: a report from the French LMB study. Pediatr Blood Cancer. 2019;66(9):e27873.

24.

Kohli

Cortes

Heaton

Dhawan

. Liver transplantation in children: state of the art and future perspectives. Arch Dis Child. 2018;103(2):192–198.

25.

Weiner

Weintraub

Wistinghausen

Tomaino

Arnon

Kerkar

Miloh

. Graft rejection in pediatric liver transplant patients with Epstein-Barr viremia and post-transplant lymphoproliferative disease. Pediatr Transplant. 2012;16(5):458–464.

26.

Wang

Chen

Cheung

Yang

Chen

Ming

Zhang

. et al. Hematopoietic chimerism in liver transplantation patients and hematopoietic stem/progenitor cells in adult human liver. Hepatology. 2012;56(4):1557–1566.

Successful Treatment of Pediatric Refractory Burkitt Lymphoma PTLD after Liver Transplantation using Anti-CD19 Chimeric Antigen Receptor T-Cell Therapy

Abstract

Keywords

Introduction

Materials and Methods

Diagnosis and Clinical History of a Child with BL-PTLD

Results

Administration of Autologous Anti-CD19 CAR T-Cells

Exceptional Tumor Response to CAR T-Cells and Stable Graft Function

Discussion

Conclusions

Footnotes

Acknowledgments

Author Contributions

Ethical Approval

Statement of Human and Animal Rights

Statement of Informed Consent

Declaration of Conflicting Interests

Funding

ORCID iD

References