Three nickel compounds were tested for pancreatic, hepatic and osteogenic damage in rats by a single i.m. injection Ni++ (7 mg kg-1). The nickel induced biochemical alterations included significantly increased levels of serum alkaline phosphatase in rats with NiS (75%) and NiO (50%). Amylase and aspartate transaminase were also increased, and lipoperoxide was increased in rats with NiO (5.6-fold) and NiS (3.4-fold). No serum changes were observed with NiCl 2. Daily injection of Cu-Zn superoxide dismutase (SOD) conjugated with polyethylene glycol pre vented the serum level changes, indicating that superoxide radical is an important intermediate in toxicity of nickel insoluble compounds.
Moulin JJ, Portefaix P., Wild P. et al. Mortality study among workers producing ferroalloys and stainless steel in France. British Journal of Industrial Medicine1990; 47: 537-43.
2.
Otto M., Snejdarkova M. & Rehat M.Hydrogen peroxide/oxygen biosensor based on supported phospholipid bilayer . Analytical Letters1992; 25: 653-62.
3.
Oskarsson A. , Andersson Y. & Tjalve H.Fate of nickel subsulfide during carcinogenesis studied by autoradiography and X-ray powder diffraction. Cancer Research1979; 39: 4175-82.
4.
Paytak PS, Aduchowski A. & Davis FFPreparation of polyethylene glycol superoxide dismutase adduct, and an examination of its blood circulating life and anti-inflammatory activity. Research Communications in Chemical Pathology and Pharmacology1980; 29: 113-27.
5.
Lowry OH, Rosembrough NJ, Farr AL et al. Protein measurement with folin-phenol reagent. Journal Biological Chemistry1951; 193: 265-75.
6.
Barber A. & Bernheim F. Lipid peroxidation: Its measurement occurrence and significance in animal tissues. Advanced Gerontology Research1967; 2: 365-403.
7.
Hausamen TU, Helger R., Rick W. et al. Optimal conditions for the determination of serum alkaline phosphatase by a new kinetic method. Clinical Chimical Acta1967; 15: 241-5.
8.
Caraway WTA stable starch substrate for the determination of amylase in serum and other body fluids. American Journal Clinical Pathology1959; 32: 97-9.
9.
Wilkinson JH , Baron DN, Moss DW et al. Standardization of clinical enzymes assays: a reference method for aspartate and alanine transaminases. Journal Clinical Pathology1972; 25: 940-9.
10.
Hopkins J. & Tudhope GRGlutathione peroxidase in human red cells in health and disease. British Journal of Haematology1973; 25: 563-75.
11.
Otero J., Toni P. & Garcia Morato YVCu-Zn superoxide dismutasa: metodo para su determinacion. Revista Iberoamericana Investigacion Clinica1983; 2: 121-7.
12.
Zer JHMultiple comparisons. In Biostatistical Analysis, McElroy WO, Swanson CD eds. New York: Prentice Hall, 1974.
13.
Novelli Elb , Rodrigues NL & Ribas BOEffect of nickel chloride on streptozotocin induced diabetes in rats. Canadian Journal of Physiology and Pharmacology1988; 66: 663-5.
14.
Vonen B. & M orland J.Isolated rat hepatocytes in suspension: potential hepatotoxic effects of six different drugs. Archives Toxicology1984; 56: 33-5.
15.
Hilmy AM, Kandeel KM & Salim NMPancreatic amylase as a tumor marker for pancreatic cancer. Archive Gesehwulstforsch1984; 54: 475-82.
16.
Nonaka A., Manabe T. & Tobe T.Effect of a new synthetic ascorbic acid derivative as a free radical scavenger on the development of acute pancreatitis in mice. Journal ofBritish Society of Gastroenterology1991; 32: 528-32.
17.
Deren JA, Kaplan FS & Brighton CTAlkaline phosphatase production by periosteal cells at various oxygen tensions in vitro. Clinical Orthopaedics and Related Research1990; 258: 307-12.
