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Abstract

Endosulfan, a well-known organochlorine pesticide, induces apoptosis and depletion of reduced glutathione (GSH) in human peripheral blood mononuclear cells (PBMC). Thus, for the amelioration of its effect, antioxidant and antiapoptotic potential of curcumin was evaluated. For ascertaining the attenuating effect of curcumin, various biochemical indices of cell damage such as cytotoxicity, oxidative stress, apoptosis (phosphatidylserine externalization, DNA fragmentation, and cytochrome c) in human PBMC was evaluated following endosulfan exposure (0–100 µM). To assess the role of HSP27 on endosulfan-induced apoptosis, the expression of HSP27 was examined. Curcumin (25 µM) increased cell viability significantly. As evident from the restoration of GSH, antiapoptotic potential was directly proportional to their antioxidant nature of curcumin. The present study indicates that the beneficial effect of curcumin on endosulfan-induced cytotoxicity is related to the induced synthesis of HSP27, emphasizing its antioxidant and therapeutic potential as well as underscoring the mechanism of pesticide-induced toxicity at cellular level. Taken together, these findings suggest that curcumin protects against endosulfan-induced immunotoxicity in human PBMC by attenuating apoptosis.

Keywords

Endosulfan curcumin apoptosis HSP27 GSH

Introduction

Curcumin is the major active component of turmeric, a yellow compound originally isolated from the plant Curcuma longa. Curcumin (diferuloylmethane) is a highly pleiotropic molecule with anti-inflammatory and antioxidant properties and modulates intracellular redox state.¹ Modulation of intracellular redox state affects a number of cellular processes since several critical transcription factors are sensitive to even minor fluctuations in the cellular redox milieu that control cell cycle, stress response, and other physiological processes.² Within the cellular context, the redox status depends on the relative amounts of the oxidized and reduced partners of major redox molecules, such as glutathione (GSH).³ Recently we have reported that curcumin (25 µM) counteracts the oxidative DNA damage in human peripheral blood mononuclear cells (PBMCs) following endosulfan exposure.⁴ In previous study, we have reported that endosulfan induces apoptosis and GSH depletion in human PBMCs.⁵

Certain chemicals such as cadmium, acrolein, dibutyltin dichloride, acetaldehyde, dexamethasone, or 2,3,7,8-tetrachlorodibenzo-p-dioxin have been reported to induce cytotoxicity of immune cells.⁶ Toxic cell damage can lead to necrosis when endogenous systems fail to compensate.⁷ Alternatively, cell death may be initiated by programmed process called apoptosis. Because of the role of apoptosis in cellular homeostasis, disorder of apoptosis results in either the accumulation of abnormal cells or the loss of cells resulting in disease pathologies like cancer, autoimmune diseases, and neurodegenerative disorders.⁸ Several studies using different cell lines have demonstrated that pesticide or pesticide mixture were able to induce apoptosis in vitro.^9,10

Studies from our laboratory have shown that endosulfan is present in breast milk, adipose tissue, placental tissue, and umbilical cord blood. We have reported significantly higher levels (4.80 ng/ml blood) of endosulfan in women with adverse reproductive outcomes in comparison with healthy women population (3.85 ng/ml blood) of North India.^11,12 Subacute and chronic toxicity studies of enosulfan on human and animals have suggested that the vital organs including liver, kidney, testes, spleen, thymus, and lymph nodes are the main target organs bearing the major impact of their toxicity.^13,14 A dose-dependent effect of endosulfan on humoral and cell-mediated immune responses in rats was reported.¹⁵ Endosulfan at 8 and 16 mg/kg, that is, 20% and 40% of median lethal doses significantly induced reduction in the anti-sheep red blood cell (anti-SRBC) antibody responses and suppressed the interferon γ and interleukin 4 cytokines levels.¹⁵ Several in vitro studies have demonstrated that endosulfan (0–100 µM) induces the cytotoxicity/apoptosis in primary culture and cell lines.^16,17 Apoptosis of neuronal cells has been one of the causes of neurotoxicity as a result of endosulfan exposure.¹⁶ Endosulfan induces oxidative stress and apoptosis in adult rat testes as a possible mechanism of action of endosulfan-induced toxicity.¹⁸ However, the consequence of prolonged exposure to subchronic doses of endosulfan at cellular level still needs elucidation.

Cells may employ several mechanisms to protect themselves from the effects of toxicants. The cellular response to stress is represented at the molecular level by the increased synthesis of heat shock proteins (HSPs). Mammalian HSP27 belongs to the family of small heat shock proteins (sHSPs) that is rapidly phosphorylated at Ser⁷⁸ and Ser⁸² in response to heat shock and other stressors, suggesting a role in the regulation of signal transduction pathways.¹⁹ The stress proteins have also been important models in various mechanistic studies like regulation of transcription, embryogenesis, aging, and apoptosis.^20,21

Earlier reports show that curcumin (0–30 µM) stimulates the stress-induced expression of genes for HSP27, αβ-crystalline and HSP70 in cultured cells and in rats tissues in vivo when injected with 20 mg/kg body weight.²² In our previous studies, we have reported that subchronic doses of pesticide induce apoptosis and also the role HSP27 in modulation of apoptosis following pesticide exposure.^23,24 This study is in line with our previous studies to demonstrate the linkage between cellular events and cell death and to evaluate the signaling mechanism revealing the specific role of HSP in endosulfan-mediated cytotoxicity.

Materials and methods

Chemicals

Endosulfan, (technical grade) was obtained from AccuStandard (New Haven, Connecticut, USA). Roswell Park Memorial Institute 1640 (RPMI-1640) media, Histopaque 1077, curcumin, and all other chemicals used in the present study were of analytical grade and purchased from Sigma–Aldrich Limited (St Louis, Missouri, USA). Stock solutions of endosulfan (99.9% purity) and curcumin (99% purity) were prepared using dimethyl sulfoxide (DMSO) and serially diluted with incomplete media (RPMI-1640 without fetal bovine serum) to prepare the 1 mM working solution. The final concentration of DMSO in working solution was up to 0.5%.

Isolation and treatment of human PBMCs

The blood samples from healthy volunteers attending blood bank of University College of Medical Sciences and GTB Hospital, Delhi, India, for blood donation were collected in heparinized tubes after obtaining informed consent per institutional ethical committee guidelines.

PBMCs were separated from peripheral blood by the density gradient centrifugation method.⁵ Briefly, 5 ml blood was layered carefully over equal volume of Histopaque 1077 and subjected to centrifugation for 30 min at 2000 r/min. PBMCs were collected from the buffy layer formed at the plasma–Histopaque 1077 interface and diluted in Hank’s balance salt solution. Cells were counted in Neubauer chamber using trypan blue and diluted with media to get a final concentration of 2 × 10⁶ cells/ml of media and incubated in 5% carbondioxide (CO₂)–95% humid atmosphere at 37°C in CO₂ incubator (MCO-15 AC, Sanyo, Japan). Cells were treated with increasing concentrations of endosulfan (0–100 μM) and along with curcumin (25 µM) for 24 h. The doses of endosulfan were based on earlier reports that endosulfan at concentration ranging from 10 µM to 100 µM induces apoptosis in Jurkat cells and in SH-SY5Y cells.^16,25 The dose of curcumin used was based on previous reports that the protective efficacy of curcumin toward HSP27 synthesis is in the range of 10–30 µM.²¹ Control cells were exposed to 0.5% DMSO.

Cytotoxicity assay

Cell viability, as an indicator of cytotoxicity, was determined by measuring the capacity of the PBMC to reduce 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Sigma Chemical Co.) to formazan. Briefly, a 5 mg/ml solution of MTT was prepared in incomplete RPMI media. MTT solution (20 µl) was added to each well of pesticide-treated PBMC and control cells in 96-well plates, followed by incubation at 37°C for 4 h in a 5% CO₂, 95% humid atmosphere. Following incubation, intracellular formazan dissolved in DMSO gave a purple color and was quantified at 540 nm using a microplate reader (Bio-Rad-680, Hercules, California, USA) with DMSO as the blank.²³ The results are presented as percentage of the control cells.

Detection of apoptosis

Annexin V-Cy3.18 binding assay

This was carried out using annexin V–Cy3.18 (AnnCy3) and 6-carboxy fluorecein diacetate (CFDA) according to the manufacturer’s protocol. Briefly, induced cells were washed twice with phosphate-buffered saline (PBS) and were suspended at a concentration approximately 1 × 10⁶ cells/ml. A circle of 1 cm diameter was drawn on a polyprep poly-l-lysine-coated slide. Fifty microliter cell suspension was added to each circle and left at room temperature for 10 min. Cells were washed twice with binding buffer and were stained with double-label staining solution containing AnnCy3 and CFDA and visualized under fluorescent microscope (Nikon-HFx-Dx, Japan) with excitation wavelength at 360 nm. Three hundred cells were counted and categorized as non-apoptotic (green), apoptotic (green and pink), and necrotic (pink) based on the staining and expressed as percentage of total cells.

DNA fragmentation assay

Cultured cells were collected by centrifugation at 1500 r/min for 5 min at 4°C. The cell pellets were washed twice with PBS, immediately lysed with 400 μl of lysis buffer (1% Triton X, 50 mM Tris–hydrochloric acid (HCl), pH 7.5, 20 mM ethylenediaminetetraacetic acid (EDTA)) and centrifuged at 4500 r/min for 5 min at 4°C in a microcentrifuge (Eppendorff). The supernatant-containing DNA was removed and incubated at 50°C for 3 h after mixing well with 20 μl of 10% sodium dodecyl sulfate (SDS) and 5 μl RNase A (10 mg/ml). After incubation, 5 μl proteinase K (15.6 mg/ml) was added, and the mixture was further incubated at 37°C for 3 h. DNA was precipitated by ethanol, separated by centrifugation and dissolved in Tris-EDTA (TE) buffer. Aliquots of DNA from different groups along with 100 bp marker were electrophoresed using 1% agarose gel containing 0.01% ethidium bromide at 80 V/18 A for 3 h. The bands were visualized and photographed using a gel documentation system (UVP DigiDoc It LS, UVP-Analytik Jena AG, Jena, Germany).

Cytochrome c assay

Cytochrome c released was measured in cell lysate by sandwich enzyme-linked immunosorbent assay (ELISA; Bender MedSystems, Vienna, Austria). Briefly, anti-human cytochrome c antibody was adsorbed onto microtiter plate to bind the cytochrome c in standard or cells that were lysed in cell extraction buffer. A 50-μl biotin-conjugated antihuman cytochrome c antibody was added and unbound biotin-conjugated antihuman cytochrome c antibody was removed by washing. Streptavidin–horseradish peroxidase (HRP, 100 μl) was added that binds to the biotin-conjugated antihuman cytochrome c antibody. Following incubation, 100 μl 3,3’,5,5’-tetramethylbenzidine substrate solution reactive with HRP was added to the wells. After enzyme reaction, a colored product formed in proportion to the amount of human cytochrome c (ng/2 × 10⁶ cells) was measured at 450 nm on microtiter plate reader (Bio-Rad-680).

Estimation of GSH

Cellular GSH was estimated spectrophotometrically in a reaction mixture containing cell lysate, nicotinamide adenine dinucleotide phosphate (NADPH) and 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB).⁵ Cell lysate was prepared by repeated freeze-thaw method using deionized water. The reaction mixture (1 ml) contained 25 μl of reduced GSH in HCl, 50 μl of NADPH (0.2 μmol/ml in 0.01M/0.005 M phosphate EDTA buffer), 10 μl of glutathione reductase (1 unit), and 800 μl of phosphate EDTA buffer (pH 7.5). The test sample was prepared by adding 25 μl of cell lysate instead of reduced GSH added in standard. The chromophoric product resulting from reaction of reagent DTNB (0.6 μmol) with GSH, that is, 2-nitro-5-thiobenzoic acid was measured spectrophotometrically at 412 nm immediately after addition of DTNB for 0–5 min time intervals. GSH concentration is expressed as percentage of control cells.

Estimation of HSP27 mRNA expression

Isolation of RNA and estimation of HSP27 expression by RT-PCR

To isolate RNA, at least 0.5 × 10⁶ cells were resuspended in Perfect RNA lysis buffer (Eppendorf, Hamburg, Germany), total RNA was extracted by Perfect RNA Eukaryotic Mini kit (Eppendorf), and the amount of extracted RNA was estimated by spectrophotometry at 260 nm. Total RNA was reverse transcribed using the high-capacity complementary DNA (cDNA) Archive Kit (Applied Biosystems, Foster City, California, USA), according to the manufacturer’s instructions. Real-time polymerasde chain reaction (PCR) was performed with an ABI prism 7000 apparatus (Applied Biosystems) using the assay on demand kits for the gene expression of HSP27. The forward and reverse-specific primer sequences of HSP27 and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) used in this study were selected from previous published data.^26,27 The thermal profile for each gene was stage 1, 2 min at 50°C; stage 2, 10 min at 95°C; stage 3, 40 cycles including 15 s at 95°C and 1 min at 60°C linearity of real-time PCR assays were tested by constructing standard curves by use of serial two fold dilutions of cDNA and regression coefficients (r ²) were always >0.900 (data not shown). Raw data were analyzed using ABI prism SDS software (Applied Biosystems). Expression of the HSP27 genes was obtained from its respective threshold cycle (C _t) value (i.e. the cycle number at which the intensity of emission of the reporter dye rises above the background noise), defined as C _t1 and normalized to the C _t of the the GAPDH (C _t2). Gene expression was finally expressed as percentage of control cells.

HSP27 level estimation

Estimation of HSP27 levels was carried out using commercially available phospho-HSP27 [pSer⁸²] ELISA kit (Sigma-Aldrich, Inc.) per manufacturer’s protocol. Briefly, washed cells were lysed in cell extraction buffer. In appropriate wells of microtiter plates, standard, sample, or controls along with anti-phospho-HSP27 [pSer⁸²] detection antibody were added (except chromogen blank) and incubated for 2 h at room temperature. Anti-rabbit immunoglobulin G-HRP was added to each well, except chromogen blanks, and incubated for 30 min at room temperature. Chromogen (100 μl) was added into the wells and incubated approximately for 30 min at room temperature. The reaction was terminated by the addition of 100 μl of stop solution, and absorbance was measured at 450 nm on microtiter plate reader (Bio-Rad-680). The results are expressed as units/2 × 10⁶ cells. One unit of standard is equivalent to the amount of phospho-HSP27 [pSer⁸²] derived from 250 pg of HSP27 that was phosphorylated by MAPKAPK-2.

Statistical analysis

All assays were repeated at least five times, and data are presented as means ± SD. Data were analyzed by repeated-measure analysis of variance using the SPSS version 17 statistical program and comparison between the various treated groups were carried out by the Tukey’s multiple comparison test. Only data with p < 0.05 were considered to be statistically significant.

Results

Effects of curcumin on survival of cells

To find out the protective effect of curcumin on endosulfan-induced cytotoxicity, PBMCs were incubated for 24 h with selected doses of endosulfan (0–100 µM), along with curcumin (25 µM) in culture media and were subsequently estimated for cell survival by MTT assay. Endosulfan showed its effects on the percentage of survival cells in dose-dependent manner. Above 10 μM of endosulfan, 10%–30% reduction in the number of surviving cells was noted. When the control cells were cotreated with curcumin, no appreciable changes were noted in cell survival. Following cotreatment with curcumin on endosulfan-treated groups, a significant improvement in the percentage of surviving cells was observed, however, no significant increase was depicted in cell survival at higher doses of pesticide (50 and 100 μM; Figure 1).

Figure 1.

Effect of curcumin on the viability of endosulfan-exposed PBMCs. PBMC culture treated with endosulfan (5–100 μM) and along with curcumin (25 μM) for 24 h. Cell viability was determined by measuring the capacity of cells to reduce MTT to intracellular purple formazan, which was quantified at 540 nm. The results are presented as percentage of survival cells. ^a p < 0.05: significantly different compared to vehicle control; ^b p < 0.05: significantly different as compared to respective endosulfan-treated group. PBMCs: peripheral blood mononuclear cells; MTT: 3-(4,5 dimethylthiazol-2yl)-2,5-diphenyltetrazoliumbromide.

Curcumin attenuates apoptosis in endosulfan exposed human PBMC

PBMCs were exposed to 0–100 µM endosulfan for 24 h and simultaneously curcumin (25 µM) was administrated. Pesticide-induced apoptosis was examined using AnnCy3 binding assay, DNA fragmentation assay, and the release of cytochrome c. Based on AnnCy3 binding assay, the cells were categorized as non-apoptotic (green), apoptotic (green and pink), and necrotic (pink) as described by us previously.^5,23,24 Similar to previous report, a significant increase in the annexin-binding cells was observed even at low concentration when incubated for 24 h (Figure 2). As reported earlier, maximum apoptosis was observed at the 20-μM concentration of endosulfan per 2 × 10⁶ PBMC. At higher concentrations, percentage of cells undergoing apoptosis declined to some extent, and cells showing necrosis increased steadily as the concentration of endosulfan increased. The electrophoretic pattern of DNA isolated from cells treated with different doses of endosulfan is shown in Figure 3. At low concentration (5 μM), no appreciable change was observed. A significant fragmentation was observed above 10 μM, indicating that the toxicity of endosulfan at lower concentration is mediated, mostly through the apoptotic effect. A dose-dependent increase in the level of cytochrome c was observed at 24 h of incubation. As depicted in Figure 3, curcumin shows its protective effect on endosufan-mediated apoptosis as observed from fragmentation pattern and significant decrease in the level of cytochrome c (Figures 3 and 4).

Figure 2.

Effect of curcumin on the apoptotic effect of endosulfan-exposed PBMCs. Values depict the percentage of cells that bind to annexin V undergoing apoptosis observed under fluorescent microscope after 24 h. ^a p < 0.05: significantly different compared to vehicle control; ^b p < 0.05: significantly different as compared to respective endosulfan-treated group. PBMCs: peripheral blood mononuclear cells.

Figure 3.

Effects of curcumin on DNA Fragmentation of PBMC after endosulfan treatment (24 h). Lane 1:100 bp marker, lane 2: 10 µM endosulfan-treated cells, lane 3: 20 µM endosulfan-treated cells, lane 4: 50 µM-treated endosulfan-treated cells, lane 5: control cells, lane 6: 20 µM endosulfan-treated cells along with 25 µM curcumin. PBMCs: peripheral blood mononuclear cells.

Figure 4.

Effect of curcumin on endosulfan-induced cytochrome c release during apoptosis of PBMCs. Levels of cytochrome c in the cell lysate are expressed as ng/2 × 10⁶ cells. ^a p < 0.05: significantly different compared to vehicle-treated control; ^b p < 0.05: significantly different as compared to respective endosulfan-treated group.

Curcumin: An inducer of HSP

To evaluate the role of curcumin as an inducer of HSP27, the expression of HSP27 was studied by real-time PCR and sandwich ELISA after exposing PBMCs to endosulfan and along with curcumin for 24 h. The expression of HSP27 messenger RNA (mRNA) was significantly increased at subchronic doses of endosulfan up to 20 μM in a dose-dependent manner. However, no significant increase in the expression of HSP27 mRNA was observed at higher concentration of endosulfan. Two and half fold increase in HSP27 mRNA expression was observed following treatment with curcumin at 20 µM endosulfan-treated group (Figure 5). The levels of HSP27 was estimated by sandwich ELISA using anti-HSP27 antibody. The similar pattern of dose-dependent effect was observed on the levels of phospho-HSP27 in these treated PBMCs (Figure 6). HSP27 expression was further enhanced significantly by the addition of curcumin (Figures 5 and 6).

Figure 5.

Expression of HSP27 mRNA in PBMCs after pesticide exposure. Total RNA was extracted from at least 0.5 × 10⁶ cells treated PBMCs. Isolated total RNA was then reverse transcribed, resulting cDNA was used as a source for amplification. Relative expression of HSP27 mRNA compared with the GAPDH housekeeping gene following exposure to endosulfan and cotreatment with curcumin was examined using real-time PCR. Data were expressed as percentage of control cells and calculated from five individual measurements for each time point. *p < 0.05: Significantly different compared with the endosulfan-treated groups. GAPDH: glyceraldehyde-3-phosphate dehydrogenase; mRNA: messenger RNA; PBMCs: peripheral blood mononuclear cells; HSP27: heat shock protein 27; cDNA: complementary DNA.

Figure 6.

Effect of curcumin on levels of HSP27 in endosulfan-treated cells. ^a p < 0.05: significantly different compared to vehicle-treated control; ^b p < 0.05: significantly different as compared to respective endosulfan-treated group. HSP27: heat shock protein 27.

Effect of curcumin on GSH level

Intracellular GSH level was estimated in endosulfan-exposed cells spectrophotometrically. Intracellular GSH level was found to be depleted in a dose-dependent manner when the cells were incubated with these pesticides for 24 h (Figure 7). Coadministration of curcumin replenished the GSH levels significantly as compared to endosulfan-treated cells (Figure 7).

Figure 7.

Effect of curcumin on GSH level in endosulfan-treated cells. *p < 0.05: Significantly different compared with the endosulfan-treated groups. GSH: glutathione.

Discussion

In this study, we found that curcumin attenuates cytotoxicity/apoptosis in human PBMCs induced by endosulfan (Figure 1), we also testified that curcumin causes the induction of HSP27. This study is in continuation of our previous study in which we had reported that endosulfan-induced apoptosis of PBMCs and depletion of reduced GSH was ascribed as an underlying mechanism.⁵ Using three different approaches in this study, namely, AnnCy3 binding assay, DNA fragmentation assay, and quantitative detection of cytochrome c, we aimed to examine the attenuating potential of curcumin on programmed cell death following low-dose exposure of endosulfan. The results from AnnCy binding assay indicates that apoptotic death process occur when the cells are exposed to endosulfan for 24 h and was found to be dose dependent (Figure 2), which is consistent with the appearance of ladder pattern of DNA fragmentation assay (Figure 3). These findings are in agreement with previous studies on induction of apoptosis by pesticides in human T cells leukemic, neuroblastoma cell lines, and murine fibroblast cells.^16,25,28 A significant dose-dependent increase in the level of cytochrome c was observed after 24 h in vitro treatment with endosulfan (Figure 4). Moreover, this significant increase in cytochrome c levels confirmed that mitochondria play an important role in pesticide-mediated apoptosis. Co-addition of curcumin significantly improved the survival of endosulfan-exposed PBMCs after 24 h incubation and rescued apoptosis of PBMCs in a dose-dependent manner (Figure 1).

The stress response is one mechanism that the cell could potentially employ to protect itself from cellular damage after exposure to xenobiotics. In doing so, they may influence the shape of the dose–response curve at low-level exposures to the environmental pollutants. The most obvious feature of the stress–response consists of acute alterations in cellular gene expression defined by a cessation of normally occurring protein synthesis and induction of the synthesis of HSPs after exposure of cells to stress.²⁹ Subsequently to withdrawal of stress, the heat shock response is downregulated, normal protein synthesis is reestablished, and the cell recovers from the stressful event. Alternatively, if the level of stress is too great to be attenuated by the stress response, cell death can ensure by necrosis and/or apoptosis. In recent years, HSPs have emerged as regulators of apoptosis. It has been suggested that HSP27 protects cells from apoptotic cell death, triggered by ionizing radiation, oxidative stress, and cytotoxic drugs.³⁰

Although widely studied in cell lines and other organ systems, expression of the stress proteins has not been studied till date in human PBMCs following endosulfan exposure. HSP27 mRNA expression in pesticide-treated PBMC revealed dose-dependent HSP synthesis at subchronic doses of endosulfan, however, there is no significant increase in the expression of HSP27 mRNA at higher concentration of endosulfan (Figure 5). The mRNA expression of HSP27 was consistent with protein levels of HSP27 (Figure 6). The results of the present study portray that HSP27 inhibits apoptosis in endosulfan-treated cells. These results are in agreement with our previous report of increased expression of HSP27 following malathion exposure.²⁴ The mechanisms by which HSPs inhibit apoptosis are still not clear. In addition to its basic function of promoting the refolding of damaged proteins and thus averting apoptosis, HSPs may directly interfere with execution of apoptosis by interacting with key components of apoptotic signaling pathway such as cytochrome c and procaspases 9 and 3.³⁰ The present data revealed HSPs may inhibit apoptosis induced by endosulfan in human PBMCs by downregulating cytochrome c release that is essential in controlling the decision to initiate apoptotic cascade. Curcumin induces HSP27 synthesis as depicted by increased mRNA expression in endosulfan-exposed PBMCs at 24 h by twofold respectively (Figure 6), thereby confirming their protective role in pesticide-mediated apoptosis. Also, reports suggest that curcumin induces nuclear translocation of the heat shock transcription factor 1, its binding to a heat shock regulatory element (HSE), and subsequent activation of the HSP promoter leading to HSP transcription.^31,32

At cellular level, measurement of reduced GSH is a marker of cellular antioxidant defense.³³ In the present study, intracellular GSH levels were found to be depleted in a dose-dependent manner in human PBMCs following endosulfan exposure for 24 h (Figure 7). A decrease in GSH alone can act as a potent early activator of apoptotic signaling. Increased ROS production following mitochondrial GSH depletion has been reported to induce apoptosis in a B cell lymphoma cell line.³³ The result obtained by us clearly indicates an association between GSH depletion, endosulfan-induced oxidative stress, and increased apoptosis of PBMC.

Antioxidants have been proven to play an important role in the regulation of a vast array of physiological and pathological processes. They principally contribute to the protection of cells and tissues against deleterious effects of reactive oxygen species and other free radicals. Several antioxidants like vitamin C, vitamin E, and N-acetylcysteine had been employed in various studies due to their antioxidant properties.^4,34 Each of the antioxidant has its own mechanism of action as well as limitations. The pharmacological safety of curcumin is well demonstrated by the fact that people in certain countries have consumed curcumin as a dietary spice for centuries, amounts in excess of 100 mg/day without any side effects.³⁵ The potential of curcumin therapy in neurological disorder such as Alzheimer’s disease has been recognized.³⁶ Studies have shown that curcumin have antiapoptotic effect.^37,38 Curcumin suppressed the activation of transcription factor nuclear factor κB in human leukemia cells.³⁹ It is a potent inhibitor of various reactive oxygen-generating enzymes, scavenges reactive oxygen species including superoxide anion radicals and hydroxyl radicals, and has also been reported to inhibit erythrocyte lipid peroxidation.⁴⁰ Curcumin also prevented free redical formation-induced myocardial ischemia and paraquat-induced lung injury in rats.⁴⁰ Moreover, curcumin protected against diazinon-induced toxicity in blood, liver, and erythrocyte of male Wistar rats.⁴¹ Futhermore, it has been reported that curcumin protect against the oxidative damage in the hippocampus of rats after exposure to parathion.⁴² Curcumin replenished the GSH levels significantly as compared to endosulfan-treated cells (Figure 7). These results are in line with our previous reports that advance attenuating capability of curcumin.^4,23,24

An in vitro study has shown that curcumin protects smooth muscle cells against cell apoptosis evoked by oxidative stress.⁴³ It has been reported that curcumin is able to induce the synthesis of HSP70 in human leukemia cells.⁴⁴ The results obtained in this study indicate that curcumin protects PBMCs from endosulfan-induced apoptosis by enhancing synthesis of HSP27. These results are in concurrence with previous studies, demonstrating that high expression of HSPs was often related to resistance to cytotoxicity and promotes cell survival by inhibiting key steps of the apoptotic cascade normally leading to cell death.^45,46

Previously, Gao et al. reported that curcumin maintained the cellular redox state via upregulation of GSH levels.⁴⁷ In this case, the resistance caused by HSP27 against endosulfan-induced apoptosis seems to be mediated, at least in part, by modulation of GSH levels. A positive correlation was observed between HSP levels and GSH depletion in endosulfan-treated cells (r = 0.007). However, at higher doses of pesticide, GSH depletion was maximum resulting in massive cell death which further leads to reduced HSP27. This suggests that induction of HSP27 protects the cell from GSH depletion. Present findings are in agreement with previous study demonstrating the GSH-dependent protective activity of HSP27 against oxidative stress.²¹ HSP27 possibly upholds GSH in its reduced form during oxidative stress.

Hence, the present study demonstrates that PBMC undergoes dose-dependent apoptotic cell death following endosulfan exposure and also highlights the effectiveness of curcumin in counteracting xenobiotic-induced cytotoxicity. These findings further support the hypothesis that oxidative stress, as indicated by GSH depletion, results in the induction of apoptosis by the release of cytochrome c. The inducible expression of HSP27 could render the human PBMCs to withstand and recover from the toxic effects of subchronic endosulfan exposure. This study emphasizes that curcumin protects cells efficiently suggesting possible therapeutic intervention in case of environmental pesticide exposure. Also, such studies will provide further insight into the molecular mechanisms behind pesticide poisoning and might suggest new diagnostic and therapeutic approaches for their toxicity and remedy.

Footnotes

Declaration of Conflicting Interests

The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

References

Srivastava

Singh

Dubey

Immunomodulatory and therapeutic activity of curcumin. Int Immunopharmacol 2011; 11(3): 331–341.

Burhans

Heintz

. The cell cycle is a redox cycle: linking phase-specific targets to cell fate. Free Radic Biol Med 2009; 47(9): 1282–1293.

Hirrlinger

Dringen

. The cytosolic redox state of astrocytes: maintenance, regulation and functional implications for metabolite trafficking. Brain Res Rev 2010; 63(1-2): 177–188.

Ahmed

Pathak

Mustafa

Ameliorating effect of N-acetylcysteine and curcumin on pesticide-induced oxidative DNA damage in human peripheral blood mononuclear cells. Environ Monit Assess 2011; 179(1-4): 293–299.

Ahmed

Tripathi

Ahmed

Endosulfan-induced apoptosis and glutathione depletion in human peripheral blood mononuclear cells: attenuation by N-acetylcysteine. J Biochem Mol Toxicol 2008; 22(5): 299–304.

Gogal

Jr Smith

Kalnitsky

Analysis of apoptosis of lymphoid cells in fish exposed to immunotoxic compounds. Cytometry 2000; 39(4): 310–318.

Paquet

Pierard

. Toxic epidermal necrolysis: revisiting the tentative link between early apoptosis and late necrosis (review). Int J Mol Med 2007; 19(1): 3–10.

Valko

Leibfritz

Moncol

Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 2007; 39(1): 44–84.

Carlson

Jortner

Ehrich

. Organophosphorus compound-induced apoptosis in SH-SY5Y human neuroblastoma cells. Toxicol Appl Pharmacol 2000; 168(2): 102–113.

10.

Saleh

Vijayasarathy

Masoud

Paraoxon induces apoptosis in EL4 cells via activation of mitochondrial pathways. Toxicol Appl Pharmacol 2003; 190(1): 47–57.

11.

Pathak

Suke

Ahmed

Endosulfan and other organochlorine pesticide residues in maternal and cord blood in North Indian population. Bull Environ Contam Toxicol 2008; 81(2): 216–219.

12.

Pathak

Ahmed

Tripathi

Maternal and cord blood levels of organochlorine pesticides: association with preterm labor. Clin Biochem 2009; 42(7-8): 746–749.

13.

Saiyed

Dewan

Bhatnagar

Effect of endosulfan on male reproductive development. Environ Health Perspect 2003; 111(16): 1958–1962.

14.

Ballesterosa

Wunderlinb

Bistonia

. Oxidative stress responses in different organs of Jenynsia multidentata exposed to endosulfan. Ecotoxicol Environ Saf 2009; 72(1): 199–205.

15.

Pal

Ahmed

Kumar

Protective effects of different antioxidants against endosulfan-induced oxidative stress and immunotoxicity in albino rats. Indian J Exp Biol 2009; 47(9): 723–729.

16.

Jia

Misra

. Exposure to mixtures of endosulfan and zineb induces apoptotic and necrotic cell death in SH-SY5Y neuroblastoma cells, in vitro . J Appl Toxicol 2007; 27(5): 434–446.

17.

Wang

Reproductive toxicity of endosulfan: Implication from germ cell apoptosis modulated by mitochondrial dysfunction and genotoxic response genes in Caenorhabditis elegans. Toxicol Sci 2015; 145(1): 118–127.

18.

Aly

Khafagy

. Taurine reverses endosulfan-induced oxidative stress and apoptosis in adult rat testis. Food Chem Toxicol 2014; 64: 1–9.

19.

Somji

Sens

Garrett

Heat shock protein 27 expression in human proximal tubule cells exposed to lethal and sublethal concentrations of CdCl₂ . Environ Health Perspect 1999; 107(7): 545–552.

20.

Filomeni

Aquilano

Rotilio

Antiapoptotic response to induced GSH depletion: involvement of heat shock proteins and NF-kappaB activation. Antioxid Redox Signal 2005; 7(3-4): 446–455.

21.

Morrow

Tanguay

. Heat shock proteins and aging in Drosophila melanogaster . Semin Cell Dev Biol 2003; 14(5): 291–299.

22.

Kato

Ito

Kamei

Stimulation of the stress-induced expression of stress proteins by curcumin in cultured cells and in rat tissues in vivo . Cell Stress Chaperones 1998; 3(3): 152–160.

23.

Ahmed

Tripathi

Ahmed

Assessment of phosphamidon-induced apoptosis in human peripheral blood mononuclear cells: protective effects of N-acetylcysteine and curcumin. J Biochem Mol Toxicol 2010; 24(5): 286–292.

24.

Ahmed

Tripathi

Suke

Role of HSP27 and reduced glutathione in modulating malathion-induced apoptosis of human peripheral blood mononuclear cells: ameliorating effect of N-acetylcysteine and curcumin. Toxicol In Vitro 2009; 23(7): 1319–1325.

25.

Kannan

Holcombe

Jain

Evidence for the induction of apoptosis by endosulfan in a human T-cell leukemic line. Mol Cell Biochem 2000; 205(1-2): 53–66.

26.

Oesterreich

Weng

Qiu

The small heat shock protein hsp27 is correlated with growth and drug resistance in human breast cancer cell lines. Cancer Res 1993; 53(19): 4443–4448.

27.

Teunissen

Koomen

de Waal Malefyt

Interleukin-17 and interferon-gamma synergize in the enhancement of proinflammatory cytokine production by human keratinocytes. J Invest Dermatol 1998; 111(4): 645–649.

28.

Masoud

Vijayasarathy

Fernandez-Cabezudo

Effect of malathion on apoptosis of murine L929 fibroblasts: a possible mechanism for toxicity in low dose exposure. Toxicology 2003; 185 (1-2): 89–102.

29.

Bernstam

Nriagu

. Molecular aspects of arsenic stress. J Toxicol Environ Health B Crit Rev 2000; 3(4): 293–322.

30.

Paul

Manero

Gonin

Hsp27 as a negative regulator of cytochrome c release. Mol Cell Biol 2002; 22(3): 816–834.

31.

Brunet Simioni

De Thonel

Hammann

Heat shock protein 27 is involved in SUMO-2/3 modification of heat shock factor 1 and thereby modulates the transcription factor activity. Oncogene 2009; 28(37): 3332–3344.

32.

Kawashima

Asai

Katagiri

Reinvestigation of the effect of carbenoxolone on the induction of heat shock proteins. Cell Stress Chaperones 2009; 14(5): 535–543.

33.

Armstrong

Steinauer

Hornung

Role of glutathione depletion and reactive oxygen species generation in apoptotic signaling in a human B lymphoma cell line. Cell Death Differ 2002; 9(3): 252–263.

34.

Takhshid

Tavasuli

Heidary

Protective effect of vitamins E and C on endosulfan-induced reproductive toxicity in male rats. Iran J Med Sci 2012; 37(3): 173–180.

35.

Ammon

Wahl

. Pharmacology of Curcuma longa . Planta Med 1991; 57(1): 1–7.

36.

Lim

Chu

Yang

The curry spice curcumin reduces oxidative damage and amyloid pathology in an Alzheimer transgenic mouse. J Neurosci 2001; 21(21): 8370–8377.

37.

Jaruga

Bielak-Zmijewska

Sikora

Glutathione-independent mechanism of apoptosis inhibition by curcumin in rat thymocytes. Biochem Pharmacol 1998; 56(8): 961–965.

38.

Somasundaram

Edmund

Moore

Dietary curcumin inhibits chemotherapy-induced apoptosis in models of human breast cancer. Cancer Res 2002; 62(13): 3868–3875.

39.

Singh

Aggarwal

. Activation of transcription factor NF-kappa B is suppressed by curcumin (diferuloylmethane). J Biol Chem 1995; 270(42): 24995–25000.

40.

Chainani-Wu

. Safety and anti-inflammatory activity of curcumin: a component of tumeric (Curcuma longa). J Altern Complement Med 2003; 9(1): 161–168.

41.

Messarah

Amamra

Boumendjel

Ameliorating effects of curcumin and vitamin E on diazinon-induced oxidative damage in rat liver and erythrocytes. Toxicol Ind Health 2013; 29(1): 77–88.

42.

Canales-Aguirre

Gomez-Pinedo

Luquin

Curcumin protects against the oxidative damage induced by the pesticide parathion in the hippocampus of the rat brain. Nutri Neurosci 2012; 15(2): 62–69.

43.

Kang

Woo

Kim

Up-regulation of aldose reductase expression mediated by phosphatidylinositol 3-kinase/Akt and Nrf2 is involved in the protective effect of curcumin against oxidative damage. Free Radic Biol Med 2007; 43(4): 535–545.

44.

Teiten

Gaascht

Eifes

Chemopreventive potential of curcumin in prostate cancer. Genes Nutr 2010; 5(1): 61–74.

45.

Beere

Green

. Stress management—heat shock protein-70 and the regulation of apoptosis. Trends Cell Biol 2001; 11(1): 6–10.

46.

Iordanskiy

Zhao

Dubrovsky

Heat shock protein 70 protects cells from cell cycle arrest and apoptosis induced by human immunodeficiency virus type 1 viral protein R. J Virol 2004; 78(18): 9697–9704.

47.

Gao

Duan

Wang

Curcumin attenuates arsenic-induced hepatic injuries and oxidative stress in experimental mice through activation of Nrf2 pathway, promotion of arsenic methylation and uninary excretion. Food Chem Toxicol 2013; 59: 739–747.

HSP27 modulates survival signaling in endosulfan-exposed human peripheral blood mononuclear cells treated with curcumin

Abstract

Keywords

Introduction

Materials and methods

Chemicals

Isolation and treatment of human PBMCs

Cytotoxicity assay

Detection of apoptosis

Annexin V-Cy3.18 binding assay

DNA fragmentation assay

Cytochrome c assay

Estimation of GSH

Estimation of HSP27 mRNA expression

Isolation of RNA and estimation of HSP27 expression by RT-PCR

HSP27 level estimation

Statistical analysis

Results

Effects of curcumin on survival of cells

Curcumin attenuates apoptosis in endosulfan exposed human PBMC

Curcumin: An inducer of HSP

Effect of curcumin on GSH level

Discussion

Footnotes

Declaration of Conflicting Interests

Funding

References