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Abstract

Cadmium (Cd) is known to cause oxidative damage in the testes of rats. The aim of this study was to investigate the protective role of rutin (RUT, 30 mg/kg) and selenium (Se, 0.15 ppm) alone or in combination against Cd (200 ppm)-induced lipid peroxidation, steroidogenesis and changes in antioxidant defence system in the rat testes. The obtained results showed that Cd increased lipid peroxidation and abnormal sperm count and decreased plasma testosterone, lactate dehydrogenase, acid phosphatase, alkaline phosphatase and testicular steroidogenic enzymes: 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD activities as well as epididymal sperm counts and motility, while RUT and Se treatment reversed this change to control values. Acute intoxication with Cd was also followed by significantly decreased activity of the antioxidant defence system (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione (GSH), and glutathione-S-transferase (GST)). Treatment with RUT and Se reversed Cd-induced alterations of antioxidant defence system and significantly prevented Cd-induced testes damage and depletion of plasma and testicular Se levels. RUT and Se appear not to have more profound effects than their separate effects against Cd-induced testicular toxicity, although Se was more potent than RUT in the recovery of testosterone levels. These results suggest that both RUT and Se do not have synergistic role against Cd-induced testicular injury.

Keywords

Cadmium selenium rutin testosterone antioxidant defence system sperms

Introduction

Cadmium (Cd) is a heavy metal, which is widely in use in batteries, dyes, plastics, electrochemistry, and paint pigment. It can be routed to soil because of the use of Cd in some insecticides, fungicides, sludge and commercial fertilizers that are used in agriculture.¹ Cd has been found to produce wide range of biochemical and physiological dysfunctions in humans and laboratory animals,² and there is sufficient evidence in humans to classify Cd and Cd compounds as carcinogenic substances.² The testis is exceedingly sensitive to Cd toxicity as reports have shown that exposure to Cd caused germ cell loss, testicular oedema, haemorrhage, necrosis and sterility in several mammalian species.^3,4 More importantly, Cd exposure may account for the recent declining fertility associated with reduced sperm count and testis function in men in developed countries.⁵ However, the cellular pathway underlying the toxic effects of Cd on the testis has not yet been clearly established.⁶

A variety of experiments have suggested that Cd causes testicular oxidative damage in animals. Primarily, Cd has a profound effect in reducing the weight of sex organs⁷ and can also directly inhibit primary Leydig cell testosterone levels.⁸ The oxidative stress and decreased testosterone level occur as a result of an increase in Cd-induced peroxidation of membrane lipids in the organs when it accumulates.^4,9 Cd also diminished the levels of several endogenous antioxidant agents in the body such as GSH and the antioxidant trace elements (Selenium (Se)) and vitamins.^10
–12 These have been shown to contribute significantly to the development of Cd-induced toxic oxidative stress.¹¹ Se has been reported to reduce and/or prevent both the oxidative stress and damage to the testis caused by Cd.^13,14 There are also many reports in which naturally occurring phytochemicals having antioxidant properties were used against Cd-induced testicular toxicity.^2,9,15 Similarly, dietary antioxidants such as Garlic¹⁶ and naringenin¹⁷ act as strong antioxidants and increase the efficacy of the chelating agent in the treatment of Cd intoxication.¹⁸ Among these phytochemicals, rutin (RUT), a citrus flavonoid glycoside present mainly in buckwheat, onions, apples, tea and red wine, has antioxidant, anti-allergic, anti-inflammatory, anti-angiogenic and antiviral properties because of its superoxide radical scavenging nature.¹¹ RUT also exhibits multiple pharmacological activities including antibacterial, anti-tumour, anti-inflammatory, anti-diarrhoeal, anti-ulcer, anti-mutagenic, myocardial protecting, vasodilator, immunomodulator and hepatoprotective activities.¹⁹ Many researchers as well as our research group have demonstrated the antioxidant properties of RUT in different experimental models.^20,21 RUT has also displayed protective effects against ethanol-induced gastric lesions,²² against DNA damage and carcinogenesis.^23,24

The Cd-induced testicular damage as well as the protective effect of RUT has not been reported in previous studies in animal models. Similarly, a joint action of both RUT and Se against cellular oxidative damage has not yet being investigated. Therefore, this study was conducted to evaluate the potential benefit of RUT and Se concomitant treatment on Cd-induced testicular pathophysiology compared to Se or RUT treatment alone in rats exposed to Cd.

Materials and methods

Animals and experimental design

Sexually mature male Wistar rats weighing approximately 139 ± 4 g were divided into six experimental groups of six animals each. The control group was not treated with Cd. The corn oil (100% pure, Vijaya Enterprises, Mumbai, India) group received 2 mL/kg body weight of corn oil. The remaining four experimental groups received 200 ppm Cd (as CdCl_2, 99.99% pure, Sigma-Aldrich, Laborchemikalien GmbH, Germany), 200 ppm Cd + 30 mg/kg RUT, 200 ppm Cd + 0.15 ppm Se (as SeO_2, 99.99% pure, Sigma-Aldrich), or 200 ppm Cd + 30 mg/kg RUT + 0.15 ppm Se. RUT (95% pure, Sigma-Aldrich, CHEMIE GmbH, Germany) was administered as a suspension in corn oil (2 mL/kg b.wt), while the Cd and Se experimental groups received Cd and/or Se in their drinking water simultaneously. Cd, Se doses and manner of administration were chosen on the basis of available literature data.^4,25 RUT dose was chosen on the basis of our previous studies.²¹ The experiment was conducted over a period of 5 weeks. The animals were housed in individual stainless steel cages and exposed to 12-h light–dark cycle. They had access to rat pellet and drinking water ad libitum. The experimental designs and protocols for study received the approval of Institutional Animal Ethics Committee in accordance with the standard guide for the care and use of laboratory animals. Drinking water consumption and daily Cd, Se intakes were investigated according to the method described by Brzóska and Moniuszko-Jakoniuk.²⁶

On the last day of experimentation, the final body weight of each animal was recorded and the rats were killed by cervical dislocation. Blood was taken by cardiac puncture and was immediately collected into heparinized tubes. Plasma samples, for testosterone, Cd and Se analyses were obtained by centrifugation at 4000 rpm for 10 min at 4°C. The testes were excised, cleared of adhering connective tissues, and weighed. The left testis was divided into two halves, the first was immediately fixed in Bouin’s solution for histopathological studies and the second was kept at −20°C for Cd and Se estimation. The right testis was processed for other biochemical estimations and protein determination.

Measurement of Cd and selenium concentrations

Testicular slices destined for Se and Cd analyses were oven dried (60^oC) to constant weight (0.07 ± 0.018 g). Dried tissues and plasma samples were digested with concentrated nitric acid (Merck, 65%) at 120°C. When fumes were white and the solution was completely clear, the samples were cooled to room temperature and the tubes were filled to 5 mL with ultra pure water. All samples were analyzed to determine Cd and Se using flame atomic absorption spectrometry. Samples were analyzed in triplicate and the variation in coefficient was usually less than 10%. Concentrations of Cd and Se were expressed as micrograms per gram dry weight in testis and as micrograms per liter in plasma.

Testosterone determination

Plasma testosterone was assayed using enzyme-linked immunosorbent assay (ELISA) kit (Equipar Diagnostici, SRL, Italy Code No. 74010) following manufacturer’s instruction. The absorbance was read at 405 nm within 30 min in ELISA reader (Merck). The intensity of the colour is inversely proportional to the concentration of testosterone in the sample. The sensitivity of the testosterone assay was 5 pg/mL, and the inter- and intra-run precision had a coefficient of variation of 3.9% and 6.2%, respectively

Epididymal sperm count, abnormal sperm count and motility

Epididymal sperm was obtained by mincing the cauda epididymis in 5 mL normal saline and filtered through a nylon mesh. An aliquot of the filtrate was used to count the number of spermatozoa in each sample using the Neubauer haemocytometer. For the estimation of spermatozoa motility, the epididymal sperm obtained from the cauda epididymis was placed on a slide and diluted with buffered 2.9% sodium citrate, pre-warmed to 37°C. The percentage motility of epididymal sperm was evaluated visually at 400× magnification within 2–4 min of their isolation from the cauda. Motility estimations were performed from three different fields in each sample. The mean was used as the final motility score. For morphological observation, one drop of the sperm suspension was smeared onto a glass slide, fixed in 95% ethanol and stained with 1% eosin and 5% nigrosine. At least 200 sperms from each rat were examined for abnormalities in different regions of spermatozoa according to the method described by Okamura et al.²⁷ The results were recorded as the percentage of abnormal sperm on each slide.

Biochemical assay

Testicular slices were homogenized in cold sodium phosphate buffer (pH 7.4). The homogenates were then centrifuged at 4000 rpm for 15 min at 4°C. The supernatants were separated and used for enzyme assays and protein determination. The activity of superoxide dismutase (SOD) using epinephrine as substrate was assayed according to the method of Misra and Fridovich.²⁸ Catalase (CAT) activity was assayed by the reduction of dichromate in acetic acid to chromic acetate when heated in the presence of hydrogen peroxide (H₂O₂). The chromic acetate produced was measured colorimetrically at 610 nm.²⁹ Glutathione-S-transferase (GST) activity was assayed by the method of Habig et al.³⁰ The specific activity was expressed as μmol GSH-CDNB (1-chloro-2,4-dinitrobenzene) conjugate formed/min/mg protein using an extinction coefficient of 9.6 mM⁻¹cm⁻¹. GSH was determined by the method of Jollow et al.³¹ where the colour developed was read at 412 nm. Glutathione peroxidase (GSH-Px) was assayed based on the reaction between glutathione (GSH) remaining after the reaction of GSH-Px and 5,5-dithiobis-(2-nitrobenzoic acid) to give a compound that absorbs light at 412 nm.³² Glutathione reductase (GR), which utilizes NADPH to convert oxidized glutathione (GSSG) to the reduced form (GSH), was assayed according to the method described in Staal et al.³³ Acid phosphatase (ACP) and alkaline phosphatase (ALP) activities were assayed according to the method described in Ketaki et al.³⁴ and was based on the hydrolysis of p-nitrophenyl phosphate. Liberated p-nitrophenyl was spectrophotometrically quantified at 420 nm. Lactate dehydrogenase (LDH) activity was determined by the method of Cabaud and Wroblewski³⁵ and was based on the interconversion of pyruvate and lactate. The activity level of testicular 3β-hydroxysteroid dehydrogenase (HSD) was measured based on the conversion of NAD to NADH while that of 17β-HSD was measured based on the conversion of NADPH to NADP.³⁶ The absorbance at 340 nm was measured at 30 s intervals for 5 min in a spectrophotometer against blank controls. The enzyme activities were expressed in mmol of NAD converted to NADH/mg protein/min (3β-HSD) and mmol of NADPH converted to NADP/mg protein/ min (17β-HSD). One unit of enzyme activity is the amount causing a change in absorbance of 0.001/min at 340 nm. Lipid peroxidation was determined by the method of Ohkawa et al.³⁷ This method was based on the reaction of malondialdehyde (MDA) and thiobarbituric acid to generate a coloured product with an absorbance at 532 nm. A standard curve using serially diluted 1,1,3,3-tetramethoxypropane was prepared and MDA concentrations in the experimental samples were extrapolated from the standard curve. Lipid peroxidation was expressed as mmol MDA/mg protein. Protein content in the tissue samples was determined by the method of Lowry et al.³⁸

Statistical analysis

All data were presented as the mean ± SD. Data were subjected to statistical analysis via one-way ANOVA. Student’s t test was utilized for comparison of data between control and experimental groups. The difference was considered to be statistically significant at p < 0.05.

Results

Effect of Se and RUT on Body and Testicular weights

As shown in Table 1, significant decreases in body and testes weights were observed as a result of Cd administration in Cd group compared to control group. Se and RUT administration alone or in combination significantly attenuated Cd-induced changes in body weight and testes weights. No significant change in body and testes weights were found in rats treated with corn oil only in comparison with the control group.

Table 1.

Changes in body weight, testis weight, plasma testosterone level and sperm characteristics in rats after Cd treatment and simultaneous supplementation with Se and RUT alone or in combination.^a

Parameters	Control	Cd	Cd + RUT	Cd + Se	Cd + RUT + Se	Corn oil
Final body weight (g)	238 ± 25^b	203 ± 6.3^c	231.25 ± 30^b	238 ± 14.4^b	236 ± 31^c	231 ± 13^b
Paired testis weight (g)	2.2 ± 0.4^b	1.3 ± 0.5^c	2.1 ± 0.7^b	2.3 ± 0.2^b	2.0 ± 0.4^b	2.3 ± 0.2^b
Testosterone (μg/L)	8.6 ± 0.7^b	4.7 ± 0.5^c	6.7 ± 0.3^d	8.4 ± 0.6^b	7.9 ± 0.4^b	8.3 ± 0.4^b
Sperm count (×10⁶/mL)	134.8 ± 7.4^b	93.8 ± 3.5^c	115 ± 10^b	108.5 ± 7.7^b	105 ± 10.4^b	136 ± 4.9^b
Sperm motility (%)	91 ± 6.52^b	68 ± 4.47^c	74 ± 8.94^b	70 ± 10^b	76 ± 5.48^b	91 ± 6.52^b
Abnormal sperm count (%)	11.9 ± 1.6^b	32.6 ± 1.2^c	12.1 ± 0.8^b	11.7 ± 0.4^b	11.5 ± 0.6^b	13.5 ± 1.1^b

RUT: rutin; Cd: cadmium; Se: selenium.

^a Mean ± SD, n = 6. Values bearing different superscript for each data point are significantly different from each other (p < 0.05).

Plasma testosterone levels, abnormal sperm count, epididymal sperm count and motility

The Cd-treated rats showed significantly lower plasma testosterone level as compared to the control animals. However, the level of plasma testosterone was reversed by the administration of Se and RUT alone or in combination. The testosterone level in the Cd + RUT + Se group was not significantly different from the Cd + Se group but was significantly increased when compared with the Cd + RUT group, indicating that Se was more effective than RUT in restoring testosterone level to near control value (Table 1). In fact, Se administration increased the plasma testosterone level by 79%, whereas RUT administration increased testosterone level by 43% when compared with Cd-treated animals. Cd treatment decreased epididymal sperm concentration and motility and increased abnormal sperm morphology rate compared with control rats. The treatment of Cd-exposed rats with Se and RUT separately or in combination prevented the Cd-induced decrease in sperm concentration, motility and increase in abnormal sperm count (Table 1). No significant change in plasma testosterone level, abnormal sperm count, epididymal sperm concentration and motility in animals treated with corn oil in comparison to the control animals was observed.

Cd and Se concentrations in plasma and testis

Our results showed that Cd administration significantly increased Cd accumulation in the plasma and testes and simultaneously decreased Se concentrations in testicular tissue and plasma in comparison with control rats. The treatment of Cd-exposed rats with Se alone had no effect on the Cd-induced increase in the plasma and testicular concentrations of Cd, whereas it reversed the Cd-induced depletion of Se concentrations in testis and plasma. The combination of RUT + Cd alone decreased the plasma and testicular Cd concentrations compared with Cd-exposed animals. This combination also prevented Cd-induced depletion of Se concentrations in the plasma and testis. The co-administration of Se + RUT in Cd-treated rats was also effective as it resulted in a significant increase in plasma and testicular Se concentrations in comparison with the Cd-treated rats and reversed the Cd-induced increase in the plasma and testicular concentrations of Cd. There were no significant differences in plasma and testicular Se and Cd concentrations between control and corn oil groups (Tables 2 and 3).

Table 2.

Se concentration in plasma and testes of rats after Cd treatment and simultaneous supplementation with Se and RUT alone or in combination.^a

Se	Control	Cd	Cd + RUT	Cd + Se	Cd + RUT + Se	corn oil
Plasma (μg/L)	0.37 ± 0.03^b	0.26 ± 0.02^c	0.31 ± 0.03^d	0.39 ± 0.01^b	0.38 ± 0.04^b	0.36 ± 0.02^b
Testis (μg/g dry weight)	3.53 ± 0.5^b	2.17 ± 0.27^c	2.86 ± 0.24^d	3.65 ± 0.17^b	3.71 ± 0.23^b	3.51 ± 0.02^b

RUT: rutin; Cd: cadmium; Se: selenium.

^a Mean ± SD, n = 6. Values bearing different superscript for each data point are significantly different from each other (p < 0.05).

Table 3.

Cd concentration in plasma and testes of rats after Cd treatment and simultaneous supplementation with Se and RUT alone or in combination^a.

Cd	Control	Cd	Cd + RUT	Cd + Se	Cd + RUT + Se	Corn oil
Plasma (μg/L)	0.03 ± 0.01^b	5.71 ± 0.42^c	3.52 ± 0.24^d	5.05 ± 0.17^c	3.49 ± 0.23^d	0.05 ± 0.02^b
Testis (μg/g dry weight)	0.04 ± 0.01^b	1.07 ± 0.15^c	0.64 ± 0.24^d	1.12 ± 0.24^c	0.67 ± 0.37^d	0.03 ± 0.01^b

RUT: rutin; Cd: cadmium; Se: selenium.

^a Mean ± SD, n = 6 for each treatment group. Values bearing different superscript for each data point are significantly different from each other (p < 0.05).

Activities of testicular steroidogenic enzymes

Testicular 3β-HSD and 17β-HSD enzymes play a pivotal role in testicular steroidogenesis. Figure 1 shows that activities of these enzymes were decreased in Cd-exposed group of rats compared with control rats. The decreased testicular 3β-HSD and 17β-HSD activities resulting from Cd treatment were reversed in animal groups treated with Se or RUT separately or in combination compared with Cd-exposed group of rats. However, we noticed that co-exposure to Cd and Se completely reversed these changes, whereas RUT supplementation only partially reversed the Cd-induced decrease in 3β-HSD and 17β-HSD activities. The activities of these enzymes were also restored completely to the control values with the combined Se and RUT treatment. No significant change in testicular 3β-HSD and 17β-HSD enzymes activities in animals treated with corn oil in comparison to the control animals were observed (Figure 1).

Figure 1.

Effects of selenium (Se) and/or rutin (RUT) on testicular steroidogenic enzyme activities namely, 17β-HSD (a) and Δ⁵3β-HSD (b) in rats after Cd treatment. (Mean ± SD, n = 6). Bars bearing different superscripts are significantly different from each other (p < 0.05).

Antioxidant defence system and lipid peroxidation in testes

Cd exposure significantly decreased the antioxidant defence system, GR, GSH-Px, GSH, GST, SOD, CAT and increased MDA concentration as compared with control rats (Control and Corn oil groups). The treatment of Cd-exposed rats with Se or RUT alone or in combination significantly increased the level of the antioxidant defence system and decreased the MDA concentration as compared with Cd-exposed rats (Figures 2 and 3). We did not observe any significant change in the antioxidant defence system and lipid peroxidation in testes of rats treated with corn oil in comparison to the control animals.

Figure 2.

Effects of selenium (Se) and/or rutin (RUT) on testicular glutathione reductase (GR) activity (a) glutathione peroxidase (GSH-Px) activity (b) reduced glutathione (GSH) level (c) glutathione-S-transferase (GST) activity (d) in rats after Cd treatment. Results are expressed as mean ± SD. n = 6 for each treatment group. Bars bearing different superscripts are significantly different from each other (p < 0.05).

Figure 3.

Effects of selenium (Se) and/or rutin (RUT) on testicular superoxide dismutase (SOD) activity (a), catalase (CAT) activity (b) and malondialdehyde (MDA) level (c) in rats after Cd treatment. Results are expressed as mean ± SD. n = 6 for each treatment group. Bars bearing different superscripts are significantly different from each other (p < 0.05).

Activities of testicular maker enzymes (marker of spermatogenesis)

Compared with the control and corn oil groups, testicular LDH, ACP and ALP, activities showed a significant decrease in the Cd-treated group. Co-treatment with Se or RUT separately or in combination effectively increased the activities of LDH, ACP and ALP toward normalcy (Table 4). There was no significant change in the activities of the testicular marker enzymes (LDH, ACP and ALP) in animals treated with corn oil in comparison to the control animals were observed.

Table 4.

Effect of Se and/or RUT on testicular LDH, ACP, and ALP activities in rats after Cd treatment.^a

Parameters	Control	Cd	Cd + RUT	Cd + Se	Cd + RUT + Se	Corn oil
LDH (Unit/mg protein)	0.21 ± 0.03^b	0.04 ± 0.05^c	0.2 ± 0.04^b	0.17 ± 0.03^b	0.18 ± 0.07^b	0.23 ± 0.04^b
ACP (Unit/mg protein)	0.26 ± 0.06^b	0.14 ± 0.03^c	0.27 ± 0.05^b	0.29 ± 0.05^a	0.3 ± 0.07^b	0.24 ± 0.06^b
ALP (Unit/mg protein)	0.19 ± 0.03^b	0.07 ± 0.01^c	0.17 ± 0.02^b	0.18 ± 0.02^b	0.16 ± 0.03^b	0.21 ± 0.02^b

RUT: rutin; Cd: cadmium; Se: selenium; LDH: lactate dehydrogenase; ACP: acid phosphatase; ALP: alkaline phosphatase.

^a Mean ± SD, n = 6 for each treatment group. Values bearing different superscripts are significantly different from each other (p < 0.05).

Histopathological study of testis

Histological study as shown in Figure 4 demonstrates normal seminiferous tubular diameter in control and corn oil-treated animals. On the other hand, shrinkage of tubular diameter was observed in Cd-treated testis. Se and RUT supplementation alone or in combination restored the shrinkage in tubular diameter.

Figure 4.

Representative photomicrograph of haematoxylin and eosin stained sections of testis of control and experimental rats (400×).

Discussion

The combined treatments of antioxidant in the prevention of Cd-induced testicular pathophysiology have been previously reported in rats.^4,14,18 In this study, we combined two potent antioxidants, a micro-nutrient, Se, and a dietary antioxidant flavonoid, RUT, to investigate their protective effects separately and then in combination against Cd-induced testicular pathophysiology in rats. In the present study, the administration of Cd significantly decreased the body weight, testis weight and the seminiferous tubular diameter. The observed weight loss of the testis may be due to the adverse effect of Cd on the number of germ cells and elongated spermatids in the testis.³⁹ The decrease in the weight of the testis and the reduced gain in body weight observed in this study are consistent with the report of others.^40
–42 Co-administration of Se + RUT to Cd-treated rats showed results approximating those in the control group with respect to testes weight, seminiferous tubular diameter and gain in body weight. The antioxidant effects of Se and RUT have been demonstrated previously in rats.^4,20,21,43 It is also reported that RUT could suppress lipid peroxidation and scavenge free radicals such as superoxide and hydroxyl radicals produced during Cd intoxication^11,44 Consistent with our results, supplementation of Se + RUT normalized the altered activities of the enzymatic and non-enzymatic antioxidant defence systems (CAT, SOD, GSH-Px, GR, GST, and GSH) in testicular tissues of Cd-treated rats. The combination of Se + RUT prevented the reduction of testicular weights in the Cd-treated rats when compared with those in Cd-alone-treated animals. The protective effects of Se + RUT can be explained by the prevention of cellular damage occurring because of oxidative stress in testicular tissues. A significantly increased accumulation of Cd in testis observed in animals treated with Cd is in accordance with previous studies.^4,25

In this study, it was observed that Cd treatment significantly decreased epididymal sperm count, motility and significantly increased abnormal sperm morphology in comparison with the control group. These results correlate with previous findings of Cd treatment in animals.^4,6,15,41 The reduction in sperm count, motility and increased abnormal sperm morphology in Cd-treated rats may be caused by lipid peroxidation of unsaturated fatty acids in the sperm plasma membrane, resulting in a loss of its fluidity and function.⁴⁰ Furthermore, overproduction of reactive oxygen species (ROS) or their accumulation due to dysfunction of the antioxidant defence system following Cd treatment leads to lipid peroxidation.^18,45 Cd is also known to bind directly to the active site of the enzyme containing –SH groups or could displace the metal cofactors from the active sites resulting in their inactivation.⁴⁶ Lipid peroxidation is a significant manifestation of oxidative damage and has been found to play an important role in the toxicity of many xenobiotics.¹⁸

Co-administration of Se + RUT alone or in combination to the Cd-treated group prevented the adverse effect of Cd on sperm characteristics, and values were close to those in the control group regarding sperm count, motility and abnormal sperm count. The obtained results correlate with the previous findings^4,21 and could be explained on the basis of the antioxidant properties of Se and RUT.

In the present study, LDH, ACP, and ALP activities showed a significant decrease after Cd administration. Since the activities of these enzymes are closely associated with spermatogenesis, the decreased activity of these enzymes in Cd-administered animals represents a defect in spermatogenesis and testicular maturation. Similar patterns of testicular LDH, ACP and ALP activities have been reported with a number of chemicals causing testicular toxicity.^2,21,47 Furthermore, the activities of ALP and ACP have also been reported to correlate with testicular steroidogenesis, suggesting that the decreased steroidogenesis was responsible for the reduction in the number of spermatids.² Administration of Cd-treated rats with Se + RUT revealed significant increase in testicular LDH, ACP and ALP when compared with the Cd-alone treated group. The obtained results agree with the previous findings,^2,21,47 suggesting that Se and RUT protected testicular function from the adverse effects of Cd.

Plasma testosterone level and testicular steroidogenic enzyme (3β-HSD and 17β-HSD) activities were significantly decreased in Cd-treated rats compared with those in the control. Our findings are in agreement with many previous studies and was thought to be related to the decreased level of gonadotrophins.^48
–50 The decrease in plasma testosterone level in Cd-treated rats may be due to the inhibition of these testicular androgenic enzyme activities, because these enzymes are responsible for the regulation of testosterone biosynthesis. RUT and Se prevented the deleterious effects of Cd on steroidogenesis as manifested by restoring the activities of the two key enzymes and plasma testosterone. The observed significant increase in plasma testosterone level in the group treated with Cd + Se is consistent with the previous reports,^4,51 confirming that Se is required for testosterone biosynthesis. The increased testosterone level in the groups treated with Cd + Se has been attributed to the antioxidant action of Se^4,51 as ROS is known to inhibit steroidogenesis.⁵² However, this is the first time we are reporting reversal of testosterone level to normalcy by RUT in Cd-induced testicular injury. The RUT-reversed Cd-induced reduction of steroidogenesis might be through scavenging of Cd-induced ROS, which can affect steroidogenic enzyme activities at protein and transcription levels.⁵² Other flavonoids with antioxidant properties as RUT have also been reported to prevent the decrease in testosterone levels in other models of testicular toxicity.^53,54 However, Se was more potent than RUT in reversing to normalcy the adverse effects of Cd on steroidogenesis. This might be due to the increased supply of Se to the testis in this group of animals. It has been reported that severely Se-deficient rats and men have lowered serum testosterone concentrations, suggesting that Se is essential for male fertility and normal development of spermatozoa.^55,56 Our observations that plasma and testicular Se concentrations were higher in Se-treated rats confirm the relevance of Se for testosterone biosynthesis.

Testicular oxidative stress is known to play an important role in male fertility. It is now known that lipid peroxidation is one of the toxic manifestations of Cd administration in testis. In the present study, the treatment of male rats with Cd resulted in a significant increase in testicular tissue levels of MDA (malondialdehyde), a by-product of lipid peroxidation, whereas GSH levels, CAT, SOD, GSH-Px, GR and GST were significantly decreased. These results correlate with previous findings of Cd treatment in animals.^4,6 The treatment of Cd-treated rats with Se + RUT revealed significant reduction in testicular tissue MDA as compared with the Cd-treated group. Se + RUT administration to Cd-treated animals significantly elevated testicular tissue GSH level and CAT, SOD, GSH-Px, GR and GST when compared with values in the Cd-alone-treated animals. The obtained results agree with the findings of others.^20,21,51,57 The reduction in lipid peroxidation in testicular tissue of Cd-treated animals because of the administration of Se + RUT alone or in combination apparently indicated that Se and RUT potently scavenged free radicals and suppressed oxidative damage.

From our study, the combination of RUT and Se did not exert stronger effect than when each was administered separately against Cd-induced testicular pathophysiology, suggesting that there was no cooperative protective action between RUT and Se against Cd-induced testicular toxicity. Therefore the combined treatments of flavonol-based phytochemicals such as RUT with antioxidant trace elements such as Se do not have greater benefits than the administration of the individual molecules during conditions of Cd-intoxication. This protective effect of RUT and Se is attributed to their antioxidant^21,51 and metal-chelating properties of RUT.⁴⁴ The capacity for Se to increase steroidogenesis better than RUT could be attributed to the increased supply of testicular Se which is required for testosterone biosynthesis. It is thus likely that testicular Se concentration could be maintained to prevent the testicular disorder caused by Cd. Supplementing with Se may improve testicular function as Se is an essential cofactor in GSH enzymatic function⁵⁸ as well as a component of selenoproteins such as glutathione peroxidase, thioredoxin/glutathione reductase which play important roles in the redox regulation of normal spermatogenesis.⁴ Furthermore, Se might also be the cornerstone of the antioxidant defence in Cd intoxication because the Cd-treated rats showed low levels of Se in the testes as well as in the plasma which were directly correlated with defects in spermatogenesis and steroidogenesis.

When the present study was initiated, the hypothesis was that the provision of a combination of endogenous antioxidant micronutrient and a dietary antioxidant (i.e. a multimodal approach) during Cd-intoxication would provide a stronger effect. For instance, Se and antioxidant vitamins such as vitamin E and vitamin C function synergistically to regenerate both water- and fat-soluble antioxidants.⁵⁹ Our results do not confirm the hypothesis that a combination strategy of antioxidant supplementation is better than single Se delivery. The reason for this could be that flavonoid–flavonoid or Se-vitamin interactions are more feasible than Se-flavonoid antioxidant interaction possibly due to a different mechanism of action.

In summary, Cd treatment increased lipid peroxidation and altered the antioxidant defence systems, resulting in oxidative damage to rat testes and decreased steroidogenesis and sperm quality. Treatment with RUT and Se alone or in combination showed protective effects against Cd-induced lipid peroxidation and changes in enzymatic and nonenzymatic (SOD, CAT, GSH-Px, GR, GST and GSH) antioxidant defence systems, steroidogenesis and sperm quality of rats. RUT and Se act as potent antioxidants alone or in combination which may be due to their free radical scavenging and metal-chelating properties. Although, RUT and Se alone and in combination did not function cooperatively in reducing the harmful effects of Cd, Se was more potent than RUT in the prevention of Cd-induced decrease in steroidogenesis. Both antioxidants can protect testicular function from Cd-induced oxidative stress.

Footnotes

Acknowledgements

The technical expertise of Mr Omoko Ejiro of the Department of Veterinary Reproduction, University of Ibadan, Oyo State on spermatozoa analysis is gratefully appreciated.

Conflict of interest

The authors declared no conflicts of interest.

Funding

This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.

References

Robards

Worsfold

. Cadmium: toxicology and analysis. A review. Analyst 1991; 116: 549–568.

Sadik

NAH

. Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium treated male rats. J Biochem Mol Toxicol 2008; 22: 345–353.

Amara

Abdelmelek

Garrel

Guiraud

Douki

Ravanat

Preventive effect of zinc against cadmium-induced oxidative stress in the rat testis. J Reprod Dev 2008; 54: 129–134.

Said

Banni

Kerkeni

Said

Messaoudi

. Influence of combined treatment with zinc and selenium on cadmium induced testicular pathphysiology in rat. Food Chem Toxicol 2010; 48: 2759–2765.

Benoff

Jacob

Hurley

. Male infertility and environmental exposure to lead and cadmium. Hum Reprod Update 2000; 6: 107–121.

Manna

Sinha

Sil

. Cadmium induced testicular pathophysiology: Prophylactic role of taurine. Reprod Toxicol 2008; 26: 282–291.

Biswas

Sengupta

Chatopadhyay

Choudhury

Sarkar

. Effect of ethanol on cadmium-induced testicular toxicity in male rats. Reprod Toxicol 2001; 15: 699–704.

Laskey

Phelps

. Effects of cadmium and other metal cations on other metal cations on in vitro leydig cell testosterone production. Toxicol Appl Pharmacol 1991; 108: 296–306.

El-Shahat

Gabr

Meki

Mehana

. Altered testicular morphology and oxidative stress induced by cadmium in experimental rats and protective effect of simultaneous green tea extract. Int J Morphol 2009; 27:757–764.

10.

El-Missiry

Shalaby

. Role of β-carotene in ameliorating the cadmium induced oxidative stress in rat brain and testis. J Biochem Mol Toxicol 2000; 14: 238–243.

11.

Mirani

Ashraf

Siddique

Rub

. Protective effect of rutin against cadmium induced hepatotoxicity in Swiss albino mice. J Pharmacol Toxicol 2012; 7: 150–157.

12.

Ognjanovic

Markovic

Dordevic

Trbojevic

Stajn

Saicic

. Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: protective role of coenzyme Q10 and Vitamin E. Reprod Toxicol 2010; 29: 191–197.

13.

Yiin

Cheru

Sheu

Lin

. Cadmium-induced lipid peroxidation in rat testes and protection by selenium. Biometals 1999; 12: 353–359.

14.

Kara

Cevik

Konar

Dayangac

Yilmaz

. Protective effects of antioxidants against cadmium-induced oxidative damage in rat testes. Biol Trace Elem Res 2007; 120: 205–211.

15.

Ola-Mudathir

Suru

Fafunso

Obioha

Faremi

. Protective roles of onion and garlic extracts on cadmium-induced changes in sperm characteristics and testicular oxidative damage in rats. Food Chem Toxicol 2008; 46: 3604–3611.

16.

Kumar

Prasad

Patra

Ranjan

Swarup

Petra

Ascorbic acid, garlic extract and taurine alleviate cadmium induced oxidative stress in freshwater catfish (Clarias batrachus). Sci Total Environ 2009; 407: 5024–5030.

17.

Renugadevi

Prabu

. Cadmium-induced hepatotoxicity in rats and the protective effect of naringenin. Exp Toxicol Pathol 2010; 62: 171–181.

18.

Gaurav

Preet

Dua

. Chronic Cadmium toxicity in rats: Treatment with combined administration of vitamins, amino acids, antioxidants and essential metals. J Food Drug Anal 2010; 18: 464–470.

19.

Janbaz

Saeed

Gilani

. Protective effect of rutin on paracetamol-and CCl₄-induced hepatotoxicity in rodents. Fitoterapia 2002; 73: 557–563.

20.

Alsaif

MA.

Combined treatment of rutin and vitamin C improves the antioxidant status in streptozotocin-induced diabetic rats.

J Med Sci 2009; 9: 1–9

21.

Abarikwu

Otuechere

Ekor

, Monwuba K and Osobu D (2012) Rutin ameliorates cyclophosphamide-induced reproductive toxicity in male rats. Toxicol Int 2012; 19: 207–214.

22.

La Casa

Villegas

de la Lastra

Motilva

Calero

. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharm 2000; 71: 45–53.

23.

Zhao

Shi

Wang

Jia

Yao

Fan

. Fast repair of deoxythymidine radical anions by two polyphenols: rutin and quercetin. Biochem Pharmacol 2003; 65: 1967–1971.

24.

Webster

Gawde

Bhattacharya

. Protective effect of rutin, a flavonol glycoside, on the carcinogen-induced DNA damage and repair enzymes in rats. Cancer Lett 1996; 109: 185–191.

25.

Messaoudi

Banni

Sai

Said

Kerkeni

. Evaluation of involvement of testicular metallothionein gene expression in the protective effect of zin against cadmium-induced testicular pathophysiology in rat. Reprod Toxicol 2010; 29: 339–345.

26.

Brzóska

Moniuszko-Jakoniuk

. Bone metabolism of male rats chronically exposed to cadmium. Toxicol Appl Pharmacol 2005; 207: 195–211.

27.

Okamura

Kamijima

Shibata

Ohtani

Takagi

Ueyem

A comprehensive evaluation of the testicular toxicity of dichlorvos in Wistar rats. Toxicol 2005; 213: 129–137.

28.

Misra

Fridovich

The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase.

J Biol Chem 1972; 247: 3170–3175

29.

Sinha

. Colorimetric assay of catalase. Anal Biochem 1972; 47: 389–394.

30.

Habig

Pabst

Jacob

. Glutathione-s-transferase. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974; 249: 7130–7139.

31.

Jollow

Mitchell

Zampaglione

Gillette

. Bromobenzene induced liver necrosis: protective role of glutathione and evidence for 3, 4 bromobenzene oxide as the hepatotoxic metabolite. Pharmacology 1974; 11: 151–169.

32.

Rotruck

Pope

Ganther

Swanson

Hafeman

Hoekstra

WG.

Selenium: biochemical role as a component of glutathione peroxidase.

Science 1973; 179: 588–590

33.

Staal

Visser

Veeger

. Purification and properties of glutathione reductase of human erythrocytes. Biochim Biophys Acta 1969; 185: 39–48.

34.

Ketaki

Dattani

Rajput

Moid

Highland

George

. Role of curcumin on chloroquine phosphate-induced reproductive toxicity. Drug Chem Toxicol 2012; 35: 184–191.

35.

Cabaud

Wroblewski

. Calorimetric measurement of lactate dehydrogenase activity of body fluids. J Clin Pathol 1958; 30: 234–236.

36.

Chandra

Chatterjee

Rituparna

Sarkar

. Vitamin E-supplementation protects chromium (VI)-induced spermatogenic and steroidogenic disorders in testicular tissues of rats. Food Chem Toxicol 2010; 48: 972–979.

37.

Ohkawa

Ohishi

Yagi

. Assay for lipid peroxides in animal tissues by thiobarbituric reaction. Anal Biochem 1979; 95: 351–358.

38.

Lowry

Rosenbrough

Farr

Randall

RJ.

Protein measurement with folin phenol reagent.

J Biol Chem 1951; 193: 265

39.

Yang

Han

Kim

Sin

. Effect of alpha-tocopherol on cadmium induced toxicity in rat testis and spermatogenesis. J Korean Med Sci 2006; 21: 445–451.

40.

El-Demerdash

Yousef

Kedwany

Bahadadi

. Cadmium induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and b-carotene. Food Chem Toxicol 2004; 42: 1563–1571.

41.

Thompson

Bannigan

. Cadmium: toxic effects on the reproductive system and the embryo. Reprod Toxicol 2008; 25: 304–315.

42.

Waisberg

Joseph

Hale

Beyersmann

. Molecular and cellular mechanisms of cadmium carcinogenesis. Toxicology 2003; 192: 95–117.

43.

Akondi

Challa

Akula

. Protective effects of rutin and naringin in testicular ischemia-reperfusion induced oxidative stress in rats. J Reprod Infertil 2011; 12: 209–214.

44.

Kamalakkannan

Prince

Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-induced diabetic wistar rats.

Basic Clin Pharmacol Toxicol 2006; 98: 97–103.

45.

Juruczuk

Brzoska

Moniuszka-Jakoniuk

Galazyn-Sidorzuk

and Eulikowska-K. Antioxidant enzymes activity and lipid peroxidation in liver and kidney of rats exposed to cadmium and ethanol. Food Chem Toxicol 2004; 42: 429–438.

46.

Casalino

Calzaretti

Sblano

Landriscina

. Cadmium dependent enzyme activity alteration is not imputable to lipid peroxidation. Arch Biochem Biophys 2000; 383: 288–295.

47.

Selvakumar

Prahalathan

Sudharsan

Varalakshmi

. Protective effect of lipoic acid on cyclophosphamide-induced testicular toxicity. Clin Chim Acta 2006; 367:114–119.

48.

Sen Gupta

Dhakal

Thakur

Ahnn

. Vitamin C and vitamin E protect rat testes from cadmium induced reactive oxygen species. Mol Cells 2003; 17: 132–139.

49.

Aktas

Kanter

Erboga

Ozturk

. Anti-apoptotic effects of curcumin on cadmium-induced apoptosis in rat testes. Toxicol Ind Health 2012; 28: 122–130.

50.

Lafuente

Marquez

Perez-Lorenzo

Pazo

Esquifino

. Cadmium effects on hypothalamic-pituitary-testicular axis in male rats. Exp Biol Med 2001; 226: 605–611.

51.

Salem

Maarouf

, Serefoglu EC and Hellstrom WJG. Selenium and lycopene attenuate cisplatin-induced testicular toxicity associated with oxidative stress in wistar rats. Urology 2012; 79: 1184.e1–1184.e6.

52.

Diemer

Allen

Hales

. Reactive oxygen disrupts mitochondria in MA-10 tumor leydig cells and inhibits steroidogenic acute regulatory (StAR) protein and steroidogenesis. Endocrinology 2003; 144: 2882–2891.

53.

Hozayen

(2012) Effect of hesperidin and rutin on doxorubicin induced testicular toxicity in male rats. Int J Food Nutr Sci 2012; 1: 31–42.

54.

Farombi

Abarikwu

Adedara

Oyeyemi

. Kolaviron and curcumin ameliorate di-n-butylphthalate-induced testicular toxicity in rats. Basic Clin Pharmacol Toxicol 2007; 100: 43–48.

55.

Hawkes

Turek

. Effects of dietary selenium on sperm motility in healthy men. J Androl 2001; 22: 764–772.

56.

Behne

Weiler

Kyriakopoulos

. Effects of selenium deficiency on testicular morphology and function in rats. J Reprod Fertil 1996; 106: 291–297.

57.

Messaoudi

Hammouda

El Heni

Baati

Saïd

Kerkeni

. Reversal of cadmium-induced oxidative stress in rat erythrocytes by selenium, zinc or their combination. Exp Toxicol Pathol 2010; 62: 281–288.

58.

Heyland

Dhaliwal

Suchner

Berger

. Antioxidant nutrients: a systematic review of trace elements and vitamins in the critically ill patient. Intens Care Med 2005; 31: 327–337.

59.

Kelly

. Vitamin E supplementation in the critically ill patient: too narrow of view? Nutr Clin Pract 1994; 9: 19–25.

Rutin- and Selenium-attenuated cadmium-induced testicular pathophysiology in rats

Abstract

Keywords

Introduction

Materials and methods

Animals and experimental design

Measurement of Cd and selenium concentrations

Testosterone determination

Epididymal sperm count, abnormal sperm count and motility

Biochemical assay

Statistical analysis

Results

Effect of Se and RUT on Body and Testicular weights

Plasma testosterone levels, abnormal sperm count, epididymal sperm count and motility

Cd and Se concentrations in plasma and testis

Activities of testicular steroidogenic enzymes

Antioxidant defence system and lipid peroxidation in testes

Activities of testicular maker enzymes (marker of spermatogenesis)

Histopathological study of testis

Discussion

Footnotes

Acknowledgements

Conflict of interest

Funding

References