Multiphase flow experiment and simulation for cells-on-a-chip devices

Abstract

A microfluidic-based microscale cell-culture device, or a cells-on-a-chip device, provides a well-controlled environment with physiologically realistic factors that emulate the organ-to-organ network of human body. In the microsystem, the in vivo situation can be resembled closely by controlling the chip geometry model, medium flow behavior, medium-to-cell density ratio, and other fluid dynamic parameters. This study is to develop multiphase models to carry out experiments and simulate flow in such devices. A standard soft lithography method is used to build the three-dimensional microfluidic chips. A definitely good qualitative and reasonably good quantitative agreement is obtained between the experimental and simulation results for particle velocity in the microfluidic chip, which validates the numerical simulation method. The cell deposition rate influenced by the flow shear is studied. The influence of gravity, inlet velocity, and cell injection number on cell concentrations are also investigated. Comparisons of different designs of cells-on-a-chip devices are addressed in the study. The physics of flow dynamics and related cell particle motion due to each of the above-mentioned variables are discussed. The results show that the multiphase flow model is promising to be used for simulating cell particle deposition and concentration for the purpose of design of cells-on-a-chip devices.