Glycosaminoglycan constituents of the periodontium have been detected in supragingival and subgingival dental calculus. They are polyanionic heteropolysaccharides containing -COOH and SO3H residues. The non-sulfated hyaluronan is present in supragingival calculus, whereas chondroitin sulfate, dermatan sulfate, and heparan sulfate are detected in subgingival calculus. They are implicated in both ectopic and endogenous mineralization, and have been used in the present study to investigate seeded hydroxyapatite crystal growth, and the influence of fluoride on the process. All glycosaminoglycans examined inhibited crystal growth, with chondroitin-4-sulfate and dermatan sulfate being more effective than the hyaluronan. Fluoride alone enhanced growth and reduced the inhibitory influence of the glycosaminoglycans and the parent proteoglycan, which is a potent inhibitor. The results yield important information on the role of fluoride and proteoglycan in ectopic mineralization.
Addadi L., Weiner S. (1985). Interactions between acidic proteins and crystals: stereochemical requirements for mineralization. Proc Natl Acad Sci USA82:4110-4114.
2.
Addadi L., Berman A., Moradian Oldak J., Weiner S. (1989). Structural and stereochemical relations between acidic macromolecules of organic matrices and crystals. Connect Tissue Res21:127-135.
3.
Amjad Z., Nancollas GH (1979). Effect of fluoride on the growth of hydroxyapatite and human dental enamel. Caries Res13:250-258.
4.
Aoba T., Moreno EC, Hay DI (1984). Inhibition of apatite crystal growth by the amino-terminal segment of salivary acidic proline-rich proteins. Calcif Tissue Int36:651-658.
5.
Barone JP, Nancollas GH (1978). The growth of calcium phosphates on hydroxyapatite crystals. The effect of fluoride and phosphonate. J Dent Res57:735-742.
6.
Blumenthal NC (1981). Mechanisms of proteoglycan inhibition of hydroxyapatite formation. In: The chemistry and biology of mineralized connective tissues. Veis A, Editor. New York: Elsevier, North Holland, pp. 509-515.
7.
Blumenthal NC, Posner AS (1987). Surface poisoning of synthetic and biological apatites. Colloids Surfaces26:123.
8.
Blumenthal NC, Posner AS, Silverman LC, Rosenberg LC (1979). The effect of proteoglycans on in vitro hydroxyapatite formation. Calcif Tissue Int27:75-82.
9.
Boskey AL, Dick BL ( 1991). Hyaluronan interactions with hydroxyapatite do not alter in vitro hydroxyapatite crystal proliferation and growth. Matrix11:442-446.
10.
Boskey AL, Maresca M., Wikstrom M., Hjerpe A. (1991). Hydroxyapatite formation in the presence of proteoglycans of reduced sulfate content: Studies in the brachymorphic mouse. Calcif Tissue Int49:389-393.
11.
Boskey AL (1994). The effects of bone proteoglycans on in vitro hydroxyapatite formation (abstract). J Dent Res73: 282.
12.
Bowness JM (1968). Present concepts of the role of ground substance in calcification. Clin Orthop Rel Res59 :233-247.
13.
Chen C-C.Boskey AL (1985). Mechanisms of proteoglycan inhibition of hydroxyapatite growth. Calcif Tissue Int37:395-400.
14.
Chen CC, Boskey AL (1986). The effect of proteoglycans from different cartilage types on in vitro hydroxyapatite proliferation. Calcif Tissue Int39:324-327.
15.
Chen C., Boskey AL, Rosenberg LC (1984). The inhibitory effect of cartilage proteoglycans on hydroxyapatite growth. Calcif Tissue Int36:285-290.
16.
Cuervo A.Pita JC, Howell DS (1973). Inhibition of calcium phosphate mineral growth by proteoglycan aggregate fractions in a synthetic lymph. Calcif Tissue Res13:1-10.
17.
Damen JJM, de Soet JJ, Ten Cate JM (1994). Adsorption of [3H]-lipoteichoic acid to hydroxyapatite and its effect on crystal growthArch Oral Biol39:753-759.
18.
Eanes ED (1980). The influence of fluoride on the seeded growth of apatite from stable supersaturated solutions at pH 7.4. J Dent Res59:144-150.
19.
Eanes ED, Hailer AW, Midura RJ, Hascall VC (1992). Proteoglycan inhibition of calcium phosphate precipitation in liposomal suspensions. Glycobiol2:571-578.
20.
Eidelman N., Chow LC, Brown WE (1987). Calcium phosphate saturation levels in ultrafiltered serum. Calcif Tissue Int40:71-78.
21.
Embery G. (1989). The organic matrix of dental calculus and its interaction with mineral. In: Recent advances in the study of dental calculus. Ten Cate JM, Editor. Oxford, UK: IRL Press, pp. 75-84.
22.
Embery G., Waddington RJ, Last K. ( 1991). The connective tissues of the periodontium and their breakdown products in the gingival sulcus fluid as markers of periodontal disease susceptibility and activity. In: Risk markers for oral disease-Markers of disease susceptibility and activity. Johnson NW, editor. Cambridge, UK: Cambridge University Press, pp. 338-364.
23.
Featherstone JDB, White DJ, Bowman WD, Shariati M., Stookey GK, Lore M., et al. (1989). Effects of anticalculus dentifrices on caries processes: in vitro and in vivo studies. In: Recent advances in the study of dental calculus. Ten Cate JM, Editor. Oxford. UK: IRL Press, pp. 197-211.
24.
Garnett J., Dieppe P. (1990). The effects of serum and human albumin on calcium hydroxyapatite crystal growth. Biochem J266:863-868.
25.
Garside J. (1982). Nucleation. In: Biological mineralization and demineralization. Nancollas GH, Editor. Berlin: Springer-Verlag, pp. 23-35.
26.
Glantz P-0 (1972). Influence of fluoride on the adhesive forces of tooth surface. In: Chemie, physikalische Chemie und Anwendungstechnik der granzflachenaktive Stoffe. Baier A , editor. Munich: Hanser, pp. 747-759.
27.
Glimcher MJ (1976). Composition, structure and organisation of bone and other mineralized tissues and the mechanism of calcification. In: Handbook of physiology: endocrinology. Greep RO, Astwood EB, Editors. Baltimore, MD: Williams and Wilkins, p. 25.
28.
Grøn P., van Campen GJ, Lindstrom I. (1967). Human dental calculus. Arch Oral Biol12:829-837.
29.
Hunter GK (1991). Role of proteoglycan in the provisional calcification of cartilage. A review and reinterpretation. Clin Orthop Rel Res262:256-280.
30.
Hunter GK, Goldberg HA (1994). Modulation of crystal formation by bone phosphoproteins: role of glutamic acid-rich sequences in the nucleation of hydroxyapatite by bone sialoprotein. Biochem J302:175-179.
31.
Hunter GK, Kyle CL, Goldberg HA (1994). Modulation of crystal growth formation by bone phosphoproteins: structural specificity of the osteopontin-mediated inhibition of hydroxyapatite formation. Biochem J300:723-728.
32.
Iijima M., Tohda H., Suzuki H., Yanagisawa T., Moriwaki Y. (1992). Effects of fluoride on apatite-octacalcium phosphate intergrowth and crystal morphology in a model system of tooth enamel formation . Calcif Tissue Int50:357-361.
33.
Kani T., Kani M., Moriwaki Y., Doi Y. (1983). Microbeam x-ray diffraction analysis of dental calculus. J Dent Res62:92-95.
34.
Kaufman HW, Kleinberg I. (1973). X-ray diffraction examination of calcium phosphate in dental plaque. Calcif Tissue Res11:97-104.
35.
Kresak M., Moreno EC, Zahradnik RT, Hay DI (1977). Adsorption of amino acids onto hydroxyapatite . J Coll Interf Sci59:283-292.
36.
Lussi A., Linde A. (1993). Mineral induction in vivo by dentin proteins. Caries Res27:241-248.
37.
Lussi A., Crenshaw MA, Linde A. (1988). Induction and inhibition of hydroxyapatite formation by rat dentin phosphoprotein in vitro. Arch Oral Biol33:685-691.
38.
Mandel ID (1987). Calculus formation and prevention: an overview . Compend Contin Educ Dent8(Spec Suppl) :235-241.
39.
Margolis HC, Varughese K., Moreno EC (1982). Effect of fluoride on crystal growth of calcium apatites in the presence of a salivary inhibitor. Calcif Tissue Int34:33-40.
40.
McDowell H., Gregory TM, Brown WE (1977). Solubility of Ca5 (PO4)3(OH) in the system Ca(OH)2-H2O at 5, 15, 25 and 37°C. J Res Natl Bur Stds81 (A):273-281.
41.
Meyer JL, Nancollas GH (1972). The effect of pH and temperature on the crystal growth of hydroxyapatite. Arch Oral Biol17:1623-1627.
42.
Meyer JL, Nancollas GH (1973). The influence of multidentate organic phosphates on the crystal growth of hydroxyapatites. Calcif Tissue Int13:295-303.
43.
Moreno EC, Kresak M., Zahradnik RT (1977). Physicochemical aspects of fluoride-apatite systems relevant to the study of dental caries. Caries Res11(Suppl 1):142-171.
44.
Moreno EC, Kresak M., Hay DI (1978). Adsorption of two human parotid salivary macromolecules on hydroxy-, fluorohydroxy- and fluorapatites. Arch Oral Biol23:525-533.
45.
Moreno EC, Varughese K., Hay DI (1979). The effect of human salivary proteins in the precipitation kinetics of calcium phosphate. Calcif Tissue Int28:7-16.
46.
Nancollas GH, Mohan MS (1970). The growth of hydroxyapatite crystals. Arch Oral Biol15:731-745.
47.
Orkin RW, Pratt RW, Martin GR (1976). Undersulfated glycosaminoglycans in the cartilage matrix of brachymorphic mice. Dev Biol50:82-94.
48.
Paschalakis P., Vynios DH, Tsiganos CP, Dalas E., Maniatis C., Koutsoukos PG (1993). Effect of proteoglycans on hydroxyapatite growth in vitro: the role of hyaluronan. Biochem Biophys Acta1158:129-136.
49.
Reddy MM, Nancollas GH (1973). Calcite crystal growth inhibition by phosphonates . Desalinization12:61-73.
50.
Rowles SL (1964). Biophysical studies on dental calculus in relation to periodontal disease. Dent Pract Dent Rec15:2-7.
51.
Schroeder HE, Bambauer HU (1966). Stages of calcium phosphate crystallisation during calculus formation. Arch Oral Biol11:1-14.
52.
Shellis RP (1988). A microcomputer programme to evaluate the saturation of complex solutions with respect to biominerals. CABIOS4:373-379.
53.
Simkiss K., Wilbur KM (1989). Biomineralization. In: Cell biology and mineral deposition. New York: Academic Press.
54.
Stookey GK, Jackson RD, Beiswanger BB, Stookey KR (1989). Clinical efficacy of chemicals for calculus prevention . In: Recent advances in the study of dental calculus. Ten Cate JM, Editor. Oxford, UK: IRL Press, pp. 235-259.
55.
Sughara K., Schwartz NB (1979). Defect in 3-phosphoadenosine-5-phosphosulfate formation in brachymorphic mice. Proc Natl Acad Sci USA76 :6615-6618.
56.
Sunberg M., Friskopp J. (1985). Crystallography of supragingival and subgingival human dental calculus. Scand J Dent Res93:30-38.
57.
Tenenbaum HC, Hunter GK (1987). Chondroitin sulfate inhibits calcification of bone formed in vitro. Bone Mineral2:43-51.
58.
Tovborg Jensen A., Dano M. (1954). Crystallography of dental calculus and the precipitation of certain calcium phosphates. J Dent Res33:741-750.
59.
Varughese K., Moreno EC (1981). Crystal growth of calcium apatites in dilute solutions containing fluoride. Calcif Tissue Int33:431-439.
60.
Waddington RJ, Embery G., Hall RC ( 1993). The influence of fluoride on proteoglycan structure using a rat odontoblast in vitro system. Calcif Tissue Int52:392-398.
61.
Williams G., Sallis JD (1982). Structural factors influencing the ability of compounds to inhibit hydroxyapatite formation. Calcif Tissue Int34:169-177.
