The seminal contributions of Dr. Edgard C. Moreno to physical chemical aspects of oral biology research are reviewed, with personal insights provided by the author. Dr. Moreno has made major contributions to our understanding of fundamental processes which occur within the oral cavity and which control the formation of mineralized tissues. These contributions include extensive research in the areas of: the chemistry of synthetic calcium phosphates and biominerals, the interaction of salivary proteins and other molecules of biological interest with calcium phosphate surfaces, the mechanism of dental caries formation and the effects of fluoride on this process, the mechanism of dental calculus formation and prevention, and the mechanism of dental enamel formation.
Aasenden R., Moreno EC (1971), Evaluation of biopsy data in human enamel fluoride studies. Arch Oral Biol16 :1413-1426.
2.
Aasenden R., Moreno EC, Brudevold F. (1972). Control of sampling areas for human tooth enamel biopsies. Arch Oral Biol17:355-358.
3.
Aasenden R., Moreno EC, Brudevold F. (1973). Fluoride levels in the surface enamel of different types of human teeth. Arch Oral Biol18:1403-1410.
4.
Aoba T., Moreno EC (1985). Adsorption of phosphoserine onto hydroxyapatite and its inhibitory activity on crystal growth. J Coll Interf Sci106:110-121.
5.
Aoba T., Moreno EC (1987). The enamel fluid in the early secretory stage of porcine amelogenesis: Chemical composition and saturation with respect to enamel mineral. Calcif Tissue Int41:86-94.
6.
Aoba T., Moreno EC, Hay DI (1984). Inhibition of apatite crystal growth by the amino-terminal segment of human salivary acidic proline-rich proteins. Calcif Tissue Int36:651-658.
7.
Aoba T., Fukae M., Tanabe T., Shimizu M., Moreno EC (1987). Selective adsorption of porcine-amelogenins onto hydroxyapatite and their inhibitory activity on hydroxyapatite growth in supersaturated solutions. Calcif Tissue Int41 :281-289.
8.
Caslavska V., Brudevold F., Moreno EC (1971). Response of the human enamel to topical application of ammonium fluoride. Arch Oral Biol16:1173-1180.
9.
DePaola PF, Brudevold F., Aasenden R., Moreno EC, Englander H., Bakhos Y., et al. (1975). A pilot study of the relationship between caries experience and surface enamel fluoride in man. Arch Oral Biol20:859-864.
10.
Gaffar A., Moreno EC (1985). Evaluation of 2-phosphono-butane 1,2,4 tricarboxylate as a crystal growth inhibitor in vitro and in vivo. J Dent Res64:6-11.
11.
Gaffar A., Moreno EC, Muhlemann HR, Niles HP (1983). Effects of EDITEMPA on dental calculus and caries formation in vivo. Calcif Tissue Int35:362-365.
12.
Gaffar A., Aoba T., Afflitto J., Esposito A., Moreno EC (1989). Structure-activity relationship between in vitro inhibition of HA crystal growth and in vivo anti-calculus effects. In: Recent advances in the study of dental calculus. ten Cate JM, editor. Oxford: IRL Press, pp. 155-173.
13.
Gibbons RJ, Moreno EC, Spinell DM (1976). Model delineating the effects of a salivary pellicle on the adsorption of Streptococcus miteor onto hydroxyapatite. Infect Immun14:1109-1112.
14.
Gibbons RJ, Moreno EC, Etherden I. (1983). Concentration-dependent multiple binding sites on saliva-treated hydroxyapatite for Streptococcus sanguis. Infect Immun39:280-289.
15.
Gibbons RJ, Etherden I., Moreno EC (1985). Contribution of stereochemical interactions in the adhesion of Streptococcus sanguis C5 to experimental pellicles. J Dent Res64:96-101.
16.
Gregory TM, Moreno EC, Brown WE (1970). Solubility of CaHPO4·2H2O in the system Ca(OH)2-H3PO4-H2O at 5, 15, 25, 37.5°C. J Res Natl Bur Stds74(A):461-475.
17.
Gregory TM, Moreno EC, Patel JM, Brown WE (1974). Solubility of Ca3(PO4)2 in the system Ca(OH)2-H3PO4-H2O at 5, 15, 25, 37°C. J Res Natl Bur Stds78(A):667-674.
18.
Hay DI, Schluckebier SK, Moreno EC (1982). Equilibrium dialysis and ultrafiltration studies of calcium and phosphate binding by human salivary proteins. Implications for salivary supersaturation with respect to calcium phosphate salts. Calcif Tissue Int34:531-538.
19.
Margolis HC, Moreno EC (1990). Physicochemical perspectives on the cariostatic mechanisms of systemic and topical fluorides. J Dent Res69(Spec Iss):606-613.
20.
Margolis HC, Moreno EC (1992). Kinetics of hydroxyapatite dissolution in acetic, lactic, and phosphoric acid solutions. Calcif Tissue Int50:137-143.
21.
Margolis HC, Moreno EC (1994). Composition and cariogenic potential of dental plaque fluid. Crit Rev Oral Biol Med5:1-25.
22.
Margolis HC, Varughese K., Moreno EC (1982). Effect of fluoride on crystal growth of calcium apatites in the presence of a salivary inhibitor. Calcif Tissue Int34:S33-S40.
23.
Margolis HC, Murphy BJ, Moreno EC (1985). Development of carious-like lesions in partially saturated lactate buffers. Caries Res19:36-45.
24.
Margolis HC, Moreno EC, Murphy BJ (1986). Effects of low levels of fluoride in solution on enamel demineralization in vitro. J Dent Res65:23-29.
25.
Moreno EC, Aoba T. ( 1987). Calcium binding in enamel fluid and driving force for enamel mineralization in the secretory stage of amelogenesis. Adv Dent Res1:245-251.
26.
Moreno EC, Varughese K. (1981). Crystal growth of calcium apatites from dilute solutions. J Cryst Growth53:20-30.
Moreno EC, Gregory TM, Brown WE (1968). Preparation and solubility of hydroxyapatite. J Res Natl Bur Stds72(A):773-782.
29.
Moreno EC, Kresak M., Zahradnik RT (1974). Fluoridated hydroxyapatite, solubility and caries formation. Nature247:64-65.
30.
Moreno EC, Kresak M., Zahradnik RT (1977). Physicochemical aspects of fluoride-apatite systems relevant to the study of dental caries. Caries Res11(Suppl 1):142-171.
31.
Moreno EC, Varughese K., Hay DI (1979). Effect of human salivary proteins on the precipitation kinetics of calcium phosphate. Calcif Tissue Int28:7-16.
32.
Moreno EC, Kresak M., Hay DI (1982). Adsorption thermodynamics of acidic proline-rich salivary proteins onto calcium apatites. J Biol Chem257:2981-2989.
33.
Moreno EC, Kresak M., Hay DI (1984). Adsorption of molecules of biological interest onto hydroxyapatite. Calcif Tissue Int36:48-59.
34.
Moreno EC, Aoba T., Margolis HC (1987a). Pyrophosphate adsorption onto hydroxyapatite and its inhibition of crystal growth. Compend Contin Educ Dent8:S256-S266.
35.
Moreno EC, Kresak M., Kane JJ, Hay DI (1987b). Adsorption of proteins, peptides and organic acids from binary mixtures onto hydroxylapatite. Langmuir3:511-519.
36.
Moreno EC, Aoba T., Gaffar A. (1989). Physical chemistry of calculus formation. In: Recent advances in the study of dental calculus. ten Cate JM, editor. Oxford: IRL Press, pp. 129-142.
37.
Moreno EC, Kresak M., Hay DI (1991). Adsorption of salivary proteins onto Ca apatites . Biofouling4:3-24.
38.
Moreno EC, Kresak M., Gaffar A. (1994). Adsorption of peroxydiphosphate onto hydroxylapatite: Kinetics, thermodynamics and crystal growth inhibition. J Coll Interf Sci168:173-182.
39.
Spinelli MA, Brudevold F., Moreno E. (1971). Mechanism of fluoride uptake by hydroxyapatite . Arch Oral Biol16:187-203.
40.
Tanaka M., Moreno EC, Margolis HC (1993). Effect of fluoride incorporation into human dental enamel on its demineralization in vitro. Arch Oral Biol38:863-869.
41.
Varughese K., Moreno EC (1981). Crystal growth of calcium apatites in dilute solutions containing fluoride. Calcif Tissue Int33:431-439.
42.
Zahradnik RT, Propas D., Moreno EC (1978). Effect of fluoride topical solutions on enamel demineralization by lactate buffers and Streptococcus mutans in vitro. J Dent Res57 :940-946.
