Biological and mechanical evaluation of mineralized-hydrogel scaffolds for tissue engineering applications

Abstract

Chitosan and gelatin have been extensively used in tissue engineering for a wide range of different applications, such as wound healing or bone regeneration, due to their advantages: excellent biocompatibility (promoting cell adhesion and proliferation), low price and biodegradability. Nonetheless, their main drawback is that they have poor mechanical properties, consequently restricting their use in bone tissue engineering. In previous studies, both materials were cross-linked, with added calcium minerals, which led to an improvement in both mechanical and biological properties. Therefore, this study carries out a mechanical and biological characterization of mineral-hydrogel scaffolds in order to find the best compositions. Different proportions of calcium compounds (CaCO₃ and CaHPO₄) are used to make up between 20% and 30% of the minerals used in a mineral-hydrogel mix. This addition of minerals enhances not only the mechanical properties, but also the biological ones. On the one hand, the higher the amount of minerals added to the composition, the better the mechanical properties obtained. Additionally, as the proportion of CaCO₃ in comparison with CaHPO₄ rises, the mechanical properties improve. On the other hand, both cell proliferation and mineralization are improved with the addition of calcium minerals.