This report demonstrates the development of a degradable quaternary ammonium derivative of chitosan (QC) composited with strontium-containing nanoapatite (SA) for bioactivity. The material was made as porous membrane by solution casting and freeze drying, for guided tissue regeneration (GTR) applications. The micromorphology, tensile strength, suture pull-out strength, degradation (in vitro, in phosphate buffered saline), and cytocompatibility (using human periodontal ligament cells) were tested to investigate the effect of derivatization and SA addition. The porosity of the membranes increased with increasing SA content and so did the tensile strength and the degradation. The suture pull-out strength, however, showed a decrease. The cell culture evaluation endorsed biocompatibility. The composite with 1.5 mg SA per 1 mL QC was found to have optimal qualities for GTR applications.
GottlowJNymanSKarringT, et al.New attachment formation as the result of controlled tissue regeneration. J Clin Periodontol1984; 11: 494–503.
2.
WangJWangLZhouZ, et al.Biodegradable polymer membranes applied in guided bone/tissue regeneration, a review. Polymers2016; 8(4): 115.
3.
ZhangYZhangXShiB, et al.Membranes for guided tissue and bone regeneration. Ann Oral Maxill Surg2013; 1(1): 10.
4.
KarringTNymanSGottlowJ, et al.Development of the biological concept of guided tissue regeneration-animal and human studies. Periodontology2000; 1(1): 26–35.
5.
SamGPillaiBRM. Evolution of barrier membranes in periodontal regeneration-“are the third generation membranes really here?J Clin Diagn Res2014; 8: ZE14–ZE17.
6.
SasakiJIAbeGLLiA, et al.Barrier membranes for tissue regeneration in dentistry. Biomater Investig Dent2021; 8: 54–63.
7.
SculeanANikolidakisDSchwarzF. Regeneration of periodontal tissues: combinations of barrier membranes and grafting materials–biological foundation and preclinical evidence: a systematic review. J Clin Periodontol2008; 35: 106–116.
8.
LiWJCooperJAMauckRL, et al.Fabrication and characterization of six electrospun poly(alpha-hydroxy ester)-based fibrous scaffolds for tissue engineering applications. Acta Biomater2006; 2: 377–385.
9.
LiaoSWangWUoM, et al.A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration. Biomaterials2005; 26: 7564–7571.
10.
FurtosGRiveroGRapunteanS, et al.Amoxicillin‐loaded electrospun nanocomposite membranes for dental applications. J Biomed Mater Res B Appl Biomater2017; 105: 966–976.
11.
Zeichner-DavidM. Is there more to enamel matrix proteins than biomineralization?Matrix Biol2001; 20: 307–316.
12.
BajićMPDanilovićVProkićB, et al.Histological effects of enamel matrix derivative on exposed dental pulp. Srp Arh Celok Lek2015; 143: 397–403.
13.
Al HezaimiKAl AskarMAl RasheedA. Characteristics of newly‐formed cementum following emdogain application. Int J Oral Sci2011; 3: 21–26.
14.
KasajAMeisterJLehmannK, et al.The influence of enamel matrix derivative on the angiogenic activity of primary endothelial cells. J Periodontal Res2012; 47: 479–487.
15.
KasajAWillershausenBReichertC, et al.Ability of nanocrystalline hydroxyapatite paste to promote human periodontal ligament cell proliferation. J Oral Sci2008; 50: 279–285.
16.
CanutoRAPolRMartinassoG, et al.Hydroxyapatite paste Ostim, without elevation of full-thickness flaps, improves alveolar healing stimulating BMP- and VEGF-mediated signal pathways: an experimental study in humans. Clin Oral Implants Res2013; 24: 42–48.
17.
RahmatiMFrankMJWalterSM, et al.Osteoimmunomodulatory effects of enamel matrix derivate and strontium coating layers: a short-and long-term in vivo study. ACS Appl Bio Mater2020; 3: 5169–5181.
18.
RohillaRTewariSNayyarAS. Efficacy of guided tissue regeneration (GTR) membranes in the healing of apico-marginal defects: a prospective, controlled clinical trial. Int J Orofacial Res2017; 2(1): 11–17.
19.
PitaruSTalHSoldingerM, et al.Partial regeneration of periodontal tissues using collagen barriers. Initial observations in the canine. J Periodontol1988; 59: 380–386.
20.
MaheshLKurtzmanGMShuklaS. Regeneration in periodontics: collagen-a review of its properties and applications in dentistry. Compend Contin Educ Dent2015; 36(5): 358–363.
21.
MadhuriSV. Membranes for periodontal regeneration. Int J Pharm Sci Invent2016; 5: 19–24.
WangWMengQLiQ, et al.Chitosan derivatives and their application in biomedicine. Int J Mol Sci2020; 21: 487.
24.
WangMMaYSunY, et al.Hierarchical porous chitosan sponges as robust and recyclable adsorbents for anionic dye adsorption. Sci Rep2017; 7: 18054.
25.
SadhasivamBRavishankarKDesinghR, et al.Biocompatible porous scaffolds of chitosan/poly (EG-ran-PG) blends with tailored pore size and nontoxic to Mesenchymal stem cells: preparation by controlled evaporation from aqueous acetic acid solution. ACS Omega2018; 3: 10286–10295.
26.
QasimSBDelaine-SmithRMFeyT, et al.Freeze gelated porous membranes for periodontal tissue regeneration. Acta Biomater2015; 23: 317–328.
27.
TanHMaRLinC, et al.Quaternized chitosan as an antimicrobial agent: antimicrobial activity, mechanism of action and biomedical applications in orthopedics. Int J Mol Sci2013; 14: 1854–1869.
28.
CapucciniCTorricelliPBoaniniE, et al.Interaction of Sr-doped hydroxyapatite nanocrystals with osteoclast and osteoblast-like cells. J Biomed Mater.Res A2009; 89: 594–600.
29.
KrishnanVBhatiaAVarmaH. Development, characterization and comparison of two strontium doped nano hydroxyapatite molecules for enamel repair/regeneration. Dent Mater2016; 32: 646–659.
30.
VargheseAGAdarshRKNishadKV, et al.In vitro evaluation of the enamel remineralization potential of a dentifrice containing nano calcium strontium apatite. Trends Biomater Artif Organs2019; 33: 56–63.
31.
PanHBLiZYLamWM, et al.Solubility of strontium-substituted apatite by solid titration. Acta Biomater2009; 5: 1678–1685.
32.
CuiDSzarpakAPignot-PaintrandI, et al.Contact-killing polyelectrolyte microcapsules based on chitosan derivatives. Adv Funct Mater2010; 20: 3303–3312.
33.
SunTKhanTHSultanaN. Fabrication and in vitro evaluation of nano sized hydroxyapatite/chitosan-based tissue engineering scaffolds. J Nanomater2014; 2014: 11.
34.
KuoSMNiuGCCLanCW, et al.Guided tissue regeneration with use of CaSO4-chitosan composite membrane. J Med Biol Eng2009; 29: 304–310.
35.
ChenTWKuoSMChangSJ, et al.Fabrication and evaluation of chitosan membranes for guided tissue regeneration. Biomed Eng Applications Basis Commun2004; 16: 259–264.
36.
ChenTWChangSJNiuGC, et al.Alginate‐coated chitosan membrane for guided tissue regeneration. J Appl Polym Sci2006; 102: 4528–4534.
37.
ZouXZhaoXYeL. Synthesis of cationic chitosan hydrogel with long chain alkyl and its controlled glucose-responsive drug delivery behavior. RSC Adv2015; 5: 96230–96241.
38.
CheungRCFNgTBWongJH, et al.Chitosan: an update on potential biomedical and pharmaceutical applications. Mar Drugs2015; 13: 5156–5186.
39.
AguilarAZeinNHarmouchE, et al.Application of chitosan in bone and dental engineering. Molecules2019; 24: 3009.
40.
GritschLMaqboolMMouriñoV, et al.Chitosan/hydroxyapatite composite bone tissue engineering scaffolds with dual and decoupled therapeutic ion delivery: Copper and strontium. J Mater Chem B2019; 7: 6109–6124.
41.
KalsiPS. Spectroscopy of organic compounds. New Delhi, India: New Age International, 2007.
42.
XianmiaoCYubaoLYiZ, et al.Properties and in vitro biological evaluation of nano-hydroxyapatite/chitosan membranes for bone guided regeneration. Mater Sci Eng C2009; 29(1): 29–35.
43.
ChenMRungeTWangL, et al.Hydrogen bonding impact on chitosan plasticization. Carbohydr Polym2018; 200: 115–121.
44.
FacasGGMaliekkalVZhuC, et al.Cooperative activation of cellulose with natural calcium. JACS Au2021; 1: 272–281.
45.
SusantoASatariMHAbbasB, et al.Fabrication and characterization of chitosan-collagen membrane from barramundi (lates calcarifer) scales for guided tissue regeneration. Eur J Dent2019; 13: 370–375.
46.
KawasakiHShimanouchiTKimuraY. Recent development of optimization of lyophilization process. J Chem2019; 2019: 9502856.
47.
RispoliLFontanaFBerettaM, et al.Surgery guidelines for barrier membranes in guided bone regeneration (GBR). J Otolaryngol Rhinol2015; 1: 1–8.
48.
GreensteinGCarpentieriJR. Utilization of D-PTFE barriers for post-extraction bone regeneration in preparation for dental implants. Compend Contin Educ Dent2015; 36(7): 465–473.
49.
TavaresDDResendeCXQuitanMP, et al.Incorporation of strontium up to 5 Mol.(%) to hydroxyapatite did not affect its cytocompatibility. Mater Res2011; 14: 456–460.
50.
YangYYangSWangY, et al.Anti-infective efficacy, cytocompatibility and biocompatibility of a 3D-printed osteoconductive composite scaffold functionalized with quaternized chitosan. Acta Biomater2016; 46: 112–128.
51.
EhretCAid-LaunaisRSagardoyT, et al.Strontium-doped hydroxyapatite polysaccharide materials effect on ectopic bone formation. PLoS One2017; 12: e0184663.
52.
IslamMMShahruzzamanMBiswasS, et al.Chitosan based bioactive materials in tissue engineering applications-A review. Bioact Mater2020; 5: 164–183.
