Traditional wound dressings are not effective enough to regulate the moisture content and remove excessive exudate from the environment. Wet wound dressings formed from hydrogels such as alginate are widely used in clinical practice for treatment of skin disorders. Here, we functionalize alginate dressings with natural antioxidants such as curcumin and t-resveratrol to render them both anti-inflammatory and antibacterial. The hydrogel maintains excellent mechanical properties and oxygen permeability over time. The release rate of the compounds from the hydrogels is assessed and their impact on bacterial and cellular growth is evaluated. The antioxidant compounds act as bactericidal agents and improve cell viability. The optimal concentration of active compounds in the engineered alginate-based dressings is determined.
WysockiABStaiano-CoicoLGrinnellF.Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9. J Invest Dermatol1993;
101: 64–68.
2.
TarnuzzerRWSchultzGS.Biochemical analysis of acute and chronic wound environments. Wound Repair Regen1996;
4: 321–325.
3.
TamayolAHassani NajafabadiAMostafaluPet al.
Biodegradable elastic nanofibrous platforms with integrated flexible heaters for on-demand drug delivery. Scientific Rep2017;
7: 9220
4.
KoontzFP.Trends in post-operative infections by Gram-positive bacteria. Int J Antimicrob Agents2000;
16: 35–37.
5.
National Guideline C. Surgical site infection: prevention and treatment of surgical site infection Rockville MD: Agency for Healthcare Research and Quality (AHRQ). Retrieved from https://www.guideline.gov/content.aspx?id=13416.
6.
SvensjoTPomahacBYaoFet al.
Accelerated healing of full-thickness skin wounds in a wet environment. Plast Reconstruct Surg2000;
106: 602–612. discussion 13–4.
7.
AtiyehBSIoannovichJAl-AmmCAet al.
Management of acute and chronic open wounds: the importance of moist environment in optimal wound healing. CPB2002;
3: 179–195.
8.
XuRXiaHHeWet al.
Controlled water vapor transmission rate promotes wound-healing via wound re-epithelialization and contraction enhancement. Scientific Rep2016;
6: 24596.
9.
SaghazadehSRinoldiCSchotMet al.
Drug delivery systems and materials for wound healing applications. Adv Drug Deliv Rev2018;
127: 138–166.
10.
Derakhshandeh H, Kashaf SS, Aghabaglou F, et al. Smart bandages: the future of wound care. Trend Biotechnol 2018; 36: 1259–1274.
11.
SeamanS.Dressing selection in chronic wound management. J Am Podiatr Med Assoc2002;
92: 24–33.
12.
SmeetsRUlrichDUnglaubFet al.
Effect of oxidised regenerated cellulose/collagen matrix on proteases in wound exudate of patients with chronic venous ulceration. Int Wound J2008;
5: 195–203.
13.
SoodAGranickMSTomaselliNL.Wound dressings and comparative effectiveness data. Adv Wound Care (New Rochelle)2014;
3: 511–529.
14.
DabiriGDamstetterEPhillipsT.Choosing a wound dressing based on common wound characteristics. Adv Wound Care (New Rochelle)2016;
5: 32–41.
15.
ZieglerUESchmidtKKellerHPet al.
Treatment of chronic wounds with an alginate dressing containing calcium zinc and manganese. Fortschritte Der Medizin Originalien2003;
121: 19–26.
16.
AnnabiNRanaDShirzaei SaniEet al.
Engineering a sprayable and elastic hydrogel adhesive with antimicrobial properties for wound healing. Biomaterials2017;
139: 229–243.
17.
BagherifardSTamayolAMostafaluPet al.
Dermal patch with integrated flexible heater for on demand drug delivery. Adv Healthcare Mater2016;
5: 175–184.
18.
EiseltPYehJLatvalaRKet al.
Porous carriers for biomedical applications based on alginate hydrogels. Biomaterials2000;
21: 1921–1927.
19.
KamataHLiXChungUIet al.
Design of hydrogels for biomedical applications. Adv Healthcare Mater2015;
4: 2360–2374.
20.
PalKBanthiaAKMajumdarDK.Hydrogels for biomedical applications: a short review. J Mater Sci Mater Med2014;
25: 2215.
21.
Mostafalu P, Kiaee G, Giatsidis G, et al. A textile dressing for temporal and dosage controlled drug delivery. Adv Funct Mater 2017; 27: 1702399.
22.
Faramarzi N, Yazdi IK, Nabavinia M, Gemma A, Fanelli A, Caizzone A, et al. Patient-Specific Bioinks for 3D Bioprinting of Tissue Engineering Scaffolds. Advanced healthcare materials 2018; 7(11): 1701347.
23.
Mostafalu P, Tamayol A, Rahimi R, et al. Smart bandage for monitoring and treatment of chronic wounds. Small 2018; 14: 1703509.
24.
WrightJBLamKBuretAGet al.
Early healing events in a porcine model of contaminated wounds: effects of nanocrystalline silver on matrix metalloproteinases, cell apoptosis, and healing. Wound Repair Regen2002;
10: 141–151.
25.
RattanaruengsrikulVPimphaNSupapholP.In vitro efficacy and toxicology evaluation of silver nanoparticle-loaded gelatin hydrogel pads as antibacterial wound dressings. J Appl Polym Sci2012;
124: 1668–1682.
26.
KhatamiMVarmaRSZafarniaNet al.
Applications of green synthesized Ag, ZnO and Ag/ZnO nanoparticles for making clinical antimicrobial wound-healing bandages. Sustain Chem Pharm2018;
10: 9–15.
27.
NasajpourAAnsariSRinoldiCet al.
A multifunctional polymeric periodontal membrane with osteogenic and antibacterial characteristics. Adv Funct Mater2018;
28: 1703437.
28.
NasajpourAMandlaSShreeSet al.
Nanostructured fibrous membranes with rose spike-like architecture. Nano Lett2017;
17: 6235–6240.
29.
QureshiMKhatoonFAhmedS.An overview on wounds, their issues and natural remedies for wound healing. Biochem Physiol2015;
4: 1453–1460.
30.
ZekaKRupareliaKCSansoneCet al.
New hydrogels enriched with antioxidants from saffron crocus can find applications in wound treatment and/or beautification. Skin Pharmacol Physiol2018;
31: 95–98.
31.
PalmieriDAliakbarianBCasazzaAAet al.
Effects of polyphenol extract from olive pomace on anoxia-induced endothelial dysfunction. Microvasc Res2012;
83: 281–289.
32.
FerrariPFPalmieriDCasazzaAAet al.
TNFα-induced endothelial activation is counteracted by polyphenol extract from UV-stressed cyanobacterium Arthrospira platensis. Med Chem Res2014;
24: 275–282.
33.
ChanMM.Antimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin. Biochem Pharmacol2002;
63: 99–104.
34.
PauloLOleastroMGallardoEet al. Antimicrobial properties of resveratrol: a review. Science against microbial pathogens: communicating current research and technological advances. 2011, pp.1225-1235.
35.
DeRKunduPSwarnakarSet al.
Antimicrobial activity of curcumin against Helicobacter pylori isolates from India and during infections in mice. Antimicrobial Agents Chemother2009;
53: 1592–1597.
36.
Mun SH, Joung DK, Kim YS, et al. Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine 2013; 20: 714–718.
37.
ChanMM-Y.Antimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin. Biochem Pharmacol2002;
63: 99–104.
38.
ChenY-CLinR-ZQiHet al.
Functional human vascular network generated in photocrosslinkable gelatin methacrylate hydrogels. Adv Funct Mater2012;
22: 2027–2039.
AliakbarianBCasazzaAAPeregoP.Valorization of olive oil solid waste using high pressure–high temperature reactor. Food Chem2011;
128: 704–710.
41.
LeeGAnandSCRajendranSet al.
Efficacy of commercial dressings in managing malodorous wounds. Br J Nurs2007;
16:S8–S20.
42.
GordilloGMSenCK.Revisiting the essential role of oxygen in wound healing. Am J Surg2003;
186: 259–263.
43.
MehtaGMehtaKSudDet al.
Quantitative measurement and control of oxygen levels in microfluidic poly(dimethylsiloxane) bioreactors during cell culture. Biomed Microdev2007;
9: 123–134.
44.
GuptaAUpadhyayNKSawhneyRCet al.
A poly-herbal formulation accelerates normal and impaired diabetic wound healing. Wound Repair Regen2008;
16: 784–790.
KantVGopalAPathakNNet al.
Antioxidant and anti-inflammatory potential of curcumin accelerated the cutaneous wound healing in streptozotocin-induced diabetic rats. Int Immunopharmacol2014;
20: 322–330.
47.
MaheshwariRKSinghAKGaddipatiJet al.
Multiple biological activities of curcumin: a short review. Life Sci2006;
78: 2081–2087.
48.
BashmakovYKAssaad-KhalilSPetyaevIM.Resveratrol may be beneficial in treatment of diabetic foot syndrome. Med Hypotheses2011;
77: 364–367.
49.
GokceEHTuncay TanriverdiSErogluIet al. Wound healing effects of collagen-laminin dermal matrix impregnated with resveratrol loaded hyaluronic acid-DPPC microparticles in diabetic rats. Eur J Pharmaceut Biopharmaceut 2017; 119: 17–27.
50.
MukherjeeSDudleyJIDasDK.Dose-dependency of resveratrol in providing health benefits. Dose Response2010;
8: 478–500.
51.
LundvigDMSPenningsSWCBrouwerKMet al.
Cytoprotective responses in HaCaT keratinocytes exposed to high doses of curcumin. Exp Cell Res2015;
336: 298–307.
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