The hydrolyzed gelatin peptides, obtained from the hydrolysis of Tilapia nilotica skin gelatin with alcalase and pronase E, were fractionated using an ultrafiltration system into hydrolyzed gelatin peptides-a (10 kDa membrane), hydrolyzed gelatin peptides-b1, and hydrolyzed gelatin peptides-b2 (5 kDa membrane) fractions. The highest oxygen radical absorbance capacity was observed in hydrolyzed gelatin peptides-b2, which contained more nonpolar amino acids than the other hydrolyzed gelatin peptides. Hydrolyzed gelatin peptides-b2 at a concentration of 12.5 mg/ml exhibited the highest proliferation ability and increased the expression of Type I procollagen mRNA, which indicated an enhanced collagen synthesis. Hydrolyzed gelatin peptides protected Detroit 551 cells from 2,2′-azobis(2-amidinopropane) dihydrochloride-induced oxidative damage and increased cell viability. Hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm were less eligible for Detroit 551 cell proliferation than bacterial cellulose. The release of hydrolyzed gelatin peptides in bacterial cellulose film was slower than that in hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm; thus, bacterial cellulose film and hydroxylpropylmethyl cellulose-modified bacterial cellulose and dried fabricated biofilm are suitable candidates for applications in delayed release type and rapid release type biofilms, respectively.
BoucardNVitonCAgayD. The use of physical hydrogels of chitosan for skin regeneration following third-degree burns. Biomaterials2007; 28: 3478–3488.
2.
StileRAHealyKE. Thermo-responsive peptide-modified hydrogels for tissue regeneration. Biomacromolecules2001; 2: 185–194.
3.
ClarkRANielsenLDWelchMP. Collagen matrices attenuate the collagen-synthetic response of cultured fibroblasts to TGF-β. J Cell Sci1995; 108: 1251–1261.
4.
HannaJRGiacopelliJA. A review of wound healing and wound dressing products. J Foot Ankle Sur1997; 36: 1–14.
5.
CzajaWKrystynowiczABieleckiS. Microbial cellulose-the natural power to heal wounds. Biomaterials2006; 27: 145–151.
6.
ManeerungTTokuraSRujiravanitR. Impregnation of silver nanoparticles into bacterial cellulose for antimicrobial wound dressing. Carbohy Pol2007; 72: 43–51.
7.
RossPMaterRBenzimanM. Cellulose biosynthesis and function in bacteria. Microbio Rev1991; 55: 35–58.
8.
KlemmDSchumannDUdhardtU. Bacterial synthesized cellulose – artificial blood vessels for microsurgery. Progress in Polymer Science2001; 26: 1561–1603.
9.
GuRKoktaBVFrankenfeldK. Bacterial cellulose reinforced thermoplastic composites: preliminary evaluation of fabrication and performance. BioResource2010; 5: 2195–2207.
10.
ChoiYJAhnYKangMS. Preparation and characterization of acrylic acid-treated bacterial cellulose cation-exchange membrane. J Chem Technol Biotechnol2004; 79: 79–84.
11.
ShahidiFAmarowiczR. Antioxidant activity of protein hydrolysates from aquatic species. J Am Oil Chem Soc1996; 73: 1197–1199.
12.
SathivelSBechtelPJBabbittJ. Biochemical and functional properties of herring (Clupeaharengus) byproduct hydrolysates. J Food Sci2003; 68: 2196–2200.
13.
MendisERajapakseNKimSK. Antioxidant properties of a radical- scavenging peptide purified from enzymatically prepared fish skin gelatin hydrolysate. J Agric Food Chem2005; 53: 581–587.
14.
SathivelSHuangJBechtelPJ. Properties of pollock (Theragra chalcogramma) skin hydrolysates and effects on lipid oxidation of skinless pink salmon (Oncorhynchus gorbuscha) fillets during 4 months of frozen storage. J Food Biochem2008; 32: 247–263.
15.
YangJILiangWSChowCJ. Process for the production of tilapia retorted skin gelatin hydrolysates with optimized antioxidative properties. Process Bio2009; 44: 1152–1157.
16.
YangRZhangZPeiX. Immunomodulatory effects of marine oligopeptide preparation from Chum Salmon (Oncorhynchus keta) in mice. Food Chem2009; 113: 464–470.
17.
ChenHHLinSBHsuCP. Modifying bacterial cellulose with gelatin peptides for improved rehydration. Cellulose2013; 20: 1967–1977.
18.
HuangHCChenLCLinSB. In-situ modification of bacterial cellulose network structure by adding interfering substances during fermentation. Biores Tech2010; 101: 6084–6091.
19.
Yang SJ. Development of fabricated composites film with bacterial cellulose and the investigation of its physical properties. Master Thesis, National Ilan University, I-Lan, Taiwan, 2011.
20.
HuWChenSLiX. In situ synthesis of silver chloride nanoparticles into bacterial cellulose membranes. Mat Sci Eng, 2009(C29): 1216–1219.
21.
RitgerPLPeppasNA. A simple equation for description of solute release. I. Fickian and non-Fickian release from non-swellable devices in the form of slabs, spheres, cylinders or discs. J Contr Rel1987; 5: 23–36.
22.
CostaPSousa LoboJM. Modeling and comparison of dissolution profiles. Eur J Pharm Sci2001; 13: 123–133.
23.
Da SilvaMABierhalzACKKieckbuscTG. Modelling natamycin release from alginate/chitosan active films. Int J Food2012; 47: 740–746.
24.
LeaneMMNankervisRSmithA. Use of the ninhydrin assay to measure the release of chitosan from oral solid dosage forms. Int J Pharmacol2004; 271: 241–249.
25.
GerlierDThomassetN. Use of MTT colorimetric assay to measure cell activation. J Immunol Methods1986; 94: 57–63.
26.
NweNFuruikeTTamuraH. Selection of a biopolymer based on attachment, morphology and proliferation of fibroblast NIH/3T3 cells for the development of a biodegradable tissue regeneration template: alginate, bacterial cellulose and gelatin. Process Biochem2010; 45: 457–466.
27.
SiZRhanjitBRoschR. Impaired balance of type I and type III procollagen mRNA in cultured fibroblasts of patients with incisional hernia. Surgery2002; 131: 324–331.
28.
CaoGPriorRL. Measurement of oxygen radical absorbance capacity in biological samples. Meth Enzym1999; 299: 50–62.
29.
MoretSPopulinTConteLS. HPLC determination of free nitrogenous compounds of Centaurea solstitialis (Asteraceae), the cause of equine nigropallidal encephalomalacia. Toxicon2005; 46: 651–657.
30.
Lai CH. Studies on the extraction of collagen from fish scales and the antioxidant activities and keratinocyte proliferation of their hydrolysates. Master thesis, National Taiwan Ocean University, Keelung, Taiwan, 2006.
31.
ChaiHJLiJHHuangHN. Effects of sizes and conformations of fish-scale collagen peptides on facial skin qualities and transdermal penetration efficiency. J Biomed Biotechnol2010; 2010: 757301–757301.
32.
Ke DY. Oxidative stress response of cultured cells to model protective effect of enzyme hydrolysates from hard clam. Master thesis, National Taiwan Ocean University, Keelung, Taiwan, 2005.
33.
KimSKKimYTByunHG. Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska Pollack skin. J Agric Food Chem2001; 49: 1984–1989.
34.
RajapakseNMendisEByunHG. Purification and in vitro antioxidative effects of giant squid muscle peptides on free radical-mediated oxidative systems. J Nutr Biochem2005; 16: 562–569.
35.
SaigaATanabeSNishimuraT. Antioxidant activity of peptides obtained from porcine myofibrillar proteins by protease treatment. J Agric Food Chem2003; 51: 3661–3667.
36.
SanchavanakitNSangrungraungrojWKaomongkolgitR. Growth of human keratinocytes and fibroblasts on bacterial cellulose film. Biotechnol Prog2006; 22: 1194–1199.
37.
LiWLZhaoSY. Functional properties of collagen hydrolysate in cosmetics. Amino Acids Biotic Res2008; 30: 10–12.
