Cytotoxicity of release products from magnetic nanocomposites in targeted drug delivery

Abstract

The efficacy of chemotherapy can be significantly improved if the therapeutic agent remains localized at the afflicted area and released at controlled rates. Such a targeted drug delivery can be achieved using magnetic nanocomposite (MNC), which incorporates drug and magnetic nanoparticles in biodegradable polymer microspheres. Reported here are results from an in vitro study on drug release rate and cytotoxicity of other release products from MNC. The model system contains an anti-cancer chemotherapy agent 5-flurouracil (5-FU) and (Co_0.5Zn_0.5)Fe₂O₄ in poly(lactic-co-glycolic acid) (PLGA) matrix produced by an oil/oil emulsion technique. Cell proliferation data indicate a sustained release of 5-FU for mouse macrophage cell eradication, whereas other microsphere components of magnetic nanoparticles and PLGA have little cytotoxic effects.

Keywords

Targeted drug delivery magnetic nanocomposites drug release rate cytotoxicity

Get full access to this article

View all access options for this article.

References

Gupta

. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications. Biomaterials 2005; 26: 3995–4021.

Longley

Harkin

Johnston

. 5-fluorouracil: mechanisms of action and clinical strategies. Nat Rev Cancer 2003; 3(5): 330–338.

Hamdeh

Oliver

Willey

Oliveri

Busca

. Magnetic properties of partially-inverted zinc ferrite aerogel powders. J Appl Phys 1997; 81(4): 1851–1857.

Hamdeh

Hikal

Taher

Thuy

Quy

. Mössbauer evaluation of cobalt ferrite nanoparticles synthesized by forced hydrolysis. J Appl Phys 2005; 97(6): 064310–4.

Jeyanthi

Rao

. Release characteristics of bleomycin mitomycin C and 5-fluorouracil from gelatin microspheres. Int J Pharm 1989; 55(1): 31–37.

Hou

Kondoh

Ohta

Gao

. Size-controlled synthesis of nickel nanoparticles. Appl Surf Sci 2005; 241: 218–222.

Asmatulu

Fakhari

Wamocha

Chu

Chen

Eltabey

. Drug-carrying magnetic nanocomposite particles for potential drug delivery systems. JNanotechnol 2009; 2009: 238536–238536. (6 pages.

Wamocha HL. Synthesis and characterization of 5-FU loaded magnetic nanocomposite spheres for advanced drug delivery. PhD Dissertation, Wichita State University, July 2010.

Basu

Katti

Kumar

. Advanced biomaterials: fundamentals, processing, and applications, Hoboken, NJ: John Wiley, 2009.

10.

DeNardo

Goldstein

Kroger

Lamborn

Levy

. Maximum-tolerated dose, toxicity, and efficacy of (131)I-Lym-1 antibody for fractionated radioimmunotherapy of non-Hodgkin's lymphoma. J Clin Oncol 1998; 16: 3246–3256.

11.

Marcos-Campos

Asín

Torres

Marquina

Tres

Ibarra

. Cell death induced by the application of alternating magnetic fields to nanoparticle-loaded dendritic cells. Nanotechnology 2011; 22(20): 205101–205101. (Epub 2011 Mar 28.

12.

Zhang

Zhou

Zhang

Cheng

Zhuo

. Sustained release of antineoplastic drugs from chitosan-reinforced alginate microparticle drug delivery systems. Int J Pharm 2008; 357(1–2): 15–21. Epub 2008 Jan 24.

13.

Panyam

Zhou

Prabha

Sahoo

Labhasetwar

. Rapid endolysosomal escape of poly (DL-lactide-co-glycolide) nanoparticles: implications for drug and gene delivery. FASEB J 2002; 16: 1217–1226.

14.

Acharya

Sahoo

. PLGA nanoparticles containing various anticancer agents and tumour delivery by EPR effect. Adv Drug Deliv Rev 2011; 63(3): 170–183. Epub 2010 Oct 20.