Dynamics of Subcutaneous Tissue Response to the Implantation of Tetracycline-Treated or Untreated Membrane of Demineralized Bovine Cortical Bone in Rats

Abstract

This study aims to conduct a histological evaluation of tissue response to a membrane obtained from demineralized bovine cortical bone, associated or not, to tetracycline (TTC). TTC treated and untreated bovine membranes were implanted in the subcutaneous tissue of rats (n = 120). The animals were killed 1, 3, 7, 15, 30, and 60 days after surgery. The tissue around the material was fixed in 10% buffered formalin for 24 h. Sections of 6 mm were stained with hematoxylin and eosin. In general, moderate to intense inflammatory response was observed in the initial periods (1 and 3 days), moderate response in the 7- and 15-day periods, and that was remarkably reduced at 30 and 60 days. Resorptions of the membranes by mononuclear cells (fibroblasts and macrophages) and multinucleated giant cells were observed 15 days after implantation. Only the remnants of the material could be detected in some animals in 60 days. Both membranes were tolerated by the tissue and were completely resorbed after 30-60 days. While the association of TTC apparently accelerated the biodegradability of the membrane substrate, no significant differences were found in the tissue response behavior between the two groups tested.

Keywords

membranes tetracycline biological barriers bovine bone

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References

Nyman, S. , Lindhe, J. , Karring, T. and Rylander, H. (1982). New Attachment Following Surgical Treatment of Human Periodontal Disease , J. Clin. Periodontol., 9(4): 290-296 .

Wang, H. L. and Macneil, R. L. (1998). Guided Tissue Regeneration. Absorbable Barriers , Dental Clinics of North America, 42(3): 505-522 .

Oh, T. J. , Meraw, S. J. , Lee, E. J. , Giannobile, W. V. and Wang, H. L. (2003). Comparative Analysis of Collagen Membranes for the Treatment of Implant Dehiscence Defects , Clin. Oral Impl. Res., 14(1): 80-90 .

Scott, T. A. , Towle, H. J. , Assad, D. A. and Nicoli, B. K. (1997). Comparison of Bioabsorbable Laminar Bone Membrane and Non-resorbable ePTFE Membrane in Mandibular Furcations , J. Periodontol., 68(7): 679-686 .

Scopp, I. W. , Kassouny, D. Y. and Morgan, F. H. (1966). Bovine Bone (Boplant) , J. Periodontol., 37(5): 400-407 .

Sbordone, L. , Barone, A. , Di Genio, A. and Ramaglia, L.S. (1999). Bacterial Colonisation during GTR Treatment. A Longitudinal Analysis , Minerva Stomatol., 48(11): 501-508 .

Chen, C. C. , Wang, H. L. , Lopatin, D. E. , O’Neal, R. B. and MacNeil, R. L. (1997). Bacterial Adherence to Guided Tissue Regeneration Barrier Membranes Exposed to the Oral Environment , J. Periodontol., 68(2): 172-179 .

Wakabayashi, R. C. , Wong, F. , Richards, D. W. and Johnson, P. W. (1996). Protease Repertoires of Cells Adherent to Membranes Recovered after Guided Tissue Regeneration , J. Periodontal. Res., 31(3): 171-180 .

Ling, L. J. , Hung, S. L. , Lee, C. F. , Chen, Y. T. and Wu, K. M. (2003). The Influence of Membrane Exposure on the Outcomes of Guided Tissue Regeneration: Clinical and Microbiological Aspects , J. Periodontal. Res., 38(1): 57-63 .

10.

Alger, F. A. , Solt, C. W. , Vuddhakanok, S. and Miles, K. (1990). The Histologic Evaluation of New Attachment in Periodontally Diseased Human Roots treated with Tetracycline-Hydrochloride and Fibronectin , J. Periodontol., 61(7): 447-455 .

11.

Drury, G. I. and Yukna, R. A. (1991). Histologic Evaluation of Combining Tetracycline and Allogeneic Freeze-dried Bone on Bone Regeneration in Experimental Defects in Baboons , J. Periodontol., 62(11): 652-658 .

12.

Rosenberg, E. and Rose, L. (1998). Biologic and Clinical Considerations for Autografts and Allografts in Periodontal Regeneration Therapy , Dental Clinics of North America, 42(3): 467-490 .

13.

Delaissé, J. M. , Engsig, M. T. , Everts, V. , del Carmen Ovejero, M. , Ferrears, M. , Lund, L. , Vu, T. H. , Werb, Z. , Winding, B. , Lochter, A. , Karsdal, M. A. , Troen, T. , Kirkegaard, T. , Lenhard, T. , Heegaard, A. M. , Neff, L. , Baron, R. and Foged, N. T. (2000). Proteinases in Bone Resorption: Obvious and Less Obvious Roles , Clinica Chimica Acta, 291(2): 223-234 .

14.

Houser, B. E. , Melloning, J. M. , Brunsvold, M. A. , Cochran, D. L. , Meffert, R. M. and Alder, M. E. (2001). Clinical Evaluation of Anorganic Bovine Bone Xenograft with a Bioabsorbable Collagen Barrier in the Treatment of Molar Furcation Defects , Int. J. Periodontics Restorative Dent., 21(2): 161-169 .

15.

Sogal, A. and Tofe, A. J. (1999). Risk Assessment of Bovine Spongiform Encephalopathy Transmission through Bone Graft Material Derived from Bovine Bone used for Dental Applications , J. Periodontol., 70(9): 1053-1063 .

16.

Wenz, B. , Oesch, B. and Horst, M. (2001) Analysis of the Risk of Transmitting Bovine Spongiform Encephalopathy through Bone Grafts Derived from Bovine Bone , Biomaterials, 22(12): 1599-1606 .

17.

Urist, M. R. (1965). Bone: Formation by Autoinduction , Science, 150(698): 893-899 .

18.

Oliveira, R. C. , Menezes, R. , Cestari, T. M. , Taga, E. M. , Taga, R. , Buzalaf, M. A. R. and Granjeiro, J. M. (2004). Tissue Response to a Membrane of Demineralized Bovine Cortical Bone Implanted in the Subcutaneous Tissue of Rats , Braz. Dent. J., 15(1): 3-8 .

19.

Zhao, S. , Pinholt, E. M. , Madsen, J. E. and Donath, K. (2000). Histological Evaluation of Diferent Biodegradable and Non-biodegradable Membranes Implanted Subcutaneously in Rats , Journal of Cranio-Maxillofacial Surgery, 28(2): 116-122 .

20.

Galgut, P. , Pitrola, R. , Waite, I. , Doyle, C. and Smith, R. (1991). Histological Evaluation of Biodegradable and Non-degradable Membranes Placed Transcutaneously in Rats , J. Clin. Periodontol., 18(8): 581-586 .

21.

Boon, M. E. , Ruijgrok, J. M. and Vardaxis, M. J. (1995). Collagen Implants Remain Supple not Allowing Fibroblast Ingrowth , Biomaterials, 16(14): 1089-1093 .

22.

Taga, R. , Hassunuma, C. Y. , Cestari, T. M. and Ferreira, P. M. (1997). Cortical Bovine Bone Membranes Placed over India Pigs Calvarium (in Portuguese) , Rev. Bras. Implant., 2(3): 24-29 .

23.

Benoit Núñez, J.S. (1999). Microscopic Analysis of the Resorption of Liofilized Cortical Bovine Bone Membranes Placed over India Pigs Calvarium with Exposed Medullar Tissue (in Portuguese), Bauru: Faculdade de Odontologia de Bauru, Universidade de São Paulo, Dissertation, p. 100.

24.

Kelly, J. D. and Schneider, G. B. (1991). Morphological and Histochemical Comparison of Cells Elicited by Ectopic Bone Implants and Tibial Osteoclasts , Am. J. Anat., 192(3): 45-54 .

25.

Oliveira, R. C. , Sicca, C. M. , Silva, T. L. , Cestari, T. M. , Oliveira, D. T. , Buzalaf, M. A. R. , Taga, R. , Taga, E. M. and Granjeiro, J. M. (1999). Effect of Desproteinization Temperature on the Preparation of Microgranular Bovine Cortical Bone. Microscopic and Biochemical Analysis in Rat Subcutaneous Tissue (in Portuguese) , Rev FOB, 7(3-4): 85-93 .

26.

Bettany, J. T. , Peet, N. M. , Wolowacz, R. G. , Skerry, T. M. and Grabowski, P. S. (2000). Tetracycline Induce Apoptosis in Osteoblasts , Bone, 27(1): 75-80 .

27.

Greenwald, R. A. , Golub, L. M. , Ramamurthy, N. S. , Chowdhury, M. , Moak, S. A. and Sorsa, T. (1998). In vitro Sensitivity of three Mammalian Collagenases to Tetracycline Inhibition: Relationship to Bone Bone and Cartilage Degradation , Bone, 22(1): 33-38 .

28.

Vernillo, A. T. and Rifkin, B. R. (1998). Effects of Tetracyclines on Bone Metabolism , Adv. Dent. Res., 12(2): 56-62 .