Abstract
The conjugation of nucleic acid base with hyaluronan was achieved by using the activated ester of pentachlorophenyl trichloroacetate. The conditions of de-N-acetylation of sodium hyaluronic acid were studied. In low concentrations of NaOH, the degree of deacetylation was 26%, while in 7.4N NaOH, the degree of deacetylation was 76% and the viscosity was 1.12 dL/g. Thymine and 5-fluorouracil bases were quantitatively conjugated to deacetylhyaluronan in 65% and 51%, respectively. The interaction of thymine hyaluronan conjugate with the complementary base of polyadenylate showed a small hypochromicity.
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