The nanocomposite hydrogels were prepared by dispersing of the nanopomegranate seed particles into poly(vinyl alcohol)/poly(acrylic acid) blend matrix in an aqueous medium by the solvent casting method. These hydrogels were characterized using scanning electron microscopy, Fourier transform infrared spectra, differential scanning calorimetry, and optical contact angle instruments. The nanopomegranate seed, blend, and hydrogel nanocomposites were tested for microbial activity. In addition, cytocompatibilities of these blend and hydrogel nanocomposites/composites were tested on human lymphocyte with in vitro MTS cell viability assays. Fourier transform infrared spectra revealed that esterification reaction took place among functional groups in the structure of poly(vinyl alcohol) and poly(acrylic acid). The hydrophilic properties of all hydrogels decreased with increasing nanopomegranate seed content. The mean diameters of the nanopomegranate seed particles were about 88 nm. Nanopomegranate seed particles demonstrated antibacterial properties against gram-positive bacteria, Staphylococcus aureus, and gram-negative bacteria, Escherichia coli. The lymphocyte viabilities increased after addition of nanopomegranate seeds into the polymer blend. The swelling behavior of blend and hydrogels was dependent on the cross-linking density created by the reaction between poly(vinyl alcohol)/poly(acrylic acid) blend and nanopomegranate seed. Scanning electron microscopy images were highly consistent with Fourier transform infrared spectra, differential scanning calorimetry, and antibacterial activity results.
JiangFTaylorDWChenJS, et al. Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage. Science2016; 19(351): 867–871.
7.
WichterleOLimD. Hydrophilic gels for biological use. Nature1960; 185: 117–118.
8.
ParadossiGCavalieriFChiessiE, et al. Poly(vinyl alcohol) as versatile biomaterial for potential biomedical applications. J Mater Sci Mater Med2003; 14: 687–691.
9.
ParkMShinHKKimBS, et al. Effect of discarded keratin-based biocomposite hydrogels on the wound healing process in vivo. Mater Sci Eng C2015; 55: 88–94.
10.
KarimiMSadeghiRKokiniJ. Pomegranate as a promising opportunity in medicine and nanotechnology. Trends Food Sci Tech2017; 69: 59–73.
11.
AtchisonJSSchauerCL. Fabrication and characterization of electrospun pristine and fluorescent composite poly (acrylic acid) ultra-fine fibers. J Eng Fiber Fabr2012; 7: 50–57.
12.
KimDSParkHBRhimJW, et al. Proton conductivity and methanol transport behavior of cross-linked PVA/PAA/silica hybrid membranes. Solid State Ionics2005; 176(1–2): 117–126.
13.
TorresHVRodrguezJVCRarnosCAC. Poly(vinyl alcohol)/Poly(acrylic acid) blends: miscibility studies by DSC and characterization of their thermally induced hydrogels. J Appl Polym Sci1993; 50: 777–792.
14.
FanXChenKHeX, et al. Nano-TiO2/collagen-chitosan porous scaffold for wound repairing. Int J Biol Macromol2016; 91: 15–22.
15.
NhiTTKhonHCHoaiNTT, et al. Fabrication of electrospun polycaprolactone coated with chitosan-silver nanoparticles membranes for wound dressing applications. J Mater Sci Mater Med2016; 27(10): 156.
16.
AgarwalANelsonTBKierskiPR, et al. Polymeric multilayers that localize the release of chlorhexidine from biologic wound dressings. Biomaterials2012; 33(28): 6783–6792.
17.
DragostinOMSamalSKDashM, et al. New antimicrobial chitosan derivatives for wound dressing applications. Carbohyd Polym2016; 141: 28–40.
18.
TavakoliJTangY. Honey/PVA hybrid wound dressings with controlled release of antibiotics structural, physico-mechanical and in-vitro biomedical studies. Mater Sci Eng C2017; 77: 318–325.
19.
NoshirvaniNGhanbarzadehBMokarramRR, et al. Preparation and characterization of active emulsified films based on chitosan-carboxymethyl cellulose containing zinc oxide nanoparticles. Int J Biol Macromol2017; 99: 530–538.
20.
DoğanEETokcanPDikenME, et al. Synthesis, characterization and some biological properties of PVA/PVP/PN hydrogel nanocomposites: antibacterial and biocompatibility. Adv Mater Sci2019; 19(3): 32–45.
21.
BeyliPTDoğanMGündüzZ, et al. Synthesis, characterization and their antimicrobial activities of boron oxide/poly(acrylic acid) nanocomposites: thermal and antimicrobial properties. Adv Mater Sci2018; 18(55): 28–36.
22.
TurhanYDoğanMAlkanM. Poly(vinyl chloride)/kaolinite nanocomposites: characterization and thermal and optical properties. Ind Eng Chem Res2010; 49(4): 1503–1513.
23.
TurhanYDoğanMAlkanM. Characterization and some properties of poly(vinyl chloride)/sepiolite nanocomposites. Adv Polym Tech2013; 32(S1): 65–82.
24.
DikenMEDoğanSTurhanY, et al. Synthesis and characterization of poly(acrylic acid)/organo-modified nanohydroxyapatite nanocomposites: thermal, optical and biocompatibility properties. Adv Mater Sci2018; 18(3): 54–67.
25.
KizildumanBKAlkanMDoğanM, et al. Al-pillared-montmorillonite (AlPMt)/Poly(methyl methacrylate) (PMMA) nanocomposites: the effects of solvent types and synthesis methods. Adv Mater Sci2017; 17(3): 5–23.
26.
BanianiDDBagheriRSoloukA. Preparation and characterization of a composite biomaterial including starch micro/nano particles loaded chitosan gel. Carbohyd Polym2017; 174: 633–645.
27.
DikenMEDoğanSTurhanY, et al. Biological properties of PMMA/nHAp and PMMA/3-APT-nHAp nanocomposites. Int J Polym Mater Po2018; 67(13): 783–791.
28.
WangMOEtheridgeJMThompsonJA, et al. Evaluation of the in vitro cytotoxicity of cross-linked biomaterials. Biomacromolecules2013; 14(5): 1321–1329.
29.
YılmazBDoğanSKasımoğullarıSÇ. Hemocompatibility, cytotoxicity, and genotoxicity of poly(methylmethacrylate)/nanohydroxyapatite nanocomposites synthesized by melt blending method. Int J Polym Mater Po2018; 67(6): 351–360.
30.
TurhanYAlpZGAlkanM, et al. Preparation and characterization of poly(vinylalcohol)/modified bentonite nanocomposites. Micropor Mesopor Mat2013; 174: 144–153.
31.
MansurHSsadahiraCMSouzaAN, et al. FTIR spectroscopy characterization of poly (vinyl alcohol) hydrogel with different hydrolysis degree and chemically crosslinked with glutaraldehyde. Mater Sci Eng C2008; 28: 539–548.
32.
AbbasizadehSKeshtkarARMousavianMA. Preparation of a novel electrospun polyvinyl alcohol/titanium oxide nanofiber adsorbent modified with mercapto groups for uranium(VI) and thorium(IV) removal from aqueous solution. Chem Eng J2013; 220: 161–171.
33.
ParkJAKangJKLeeSC, et al. Electrospun poly(acrylic acid)/poly(vinyl alcohol) nanofibrous adsorbents for Cu(II) removal from industrial plating wastewater. RSC Adv2017; 7: 18075–18084.
34.
Cauich-RodriguezJVDebSSmithR. Effect of cross-linking agents on the dynamic mechanical properties of hydrogel blends of poly(acrylic acid)- poly(viny1 alcohol-vinyl acetate). Biomaterials1996; 96(17): 58–2259.
35.
ParkHCMeertensRMMulderMHV, et al. Pervaporation of alcohol-toluene mixtures through polymer blend membranes of poly (acrylic acid) and poly (vinyl alcohol). J Membrane Sci1994; 90: 265–274.
36.
ChaudhariSKwonYSMoonMJ, et al. In situ generation of silver nanoparticles in poly(vinyl alcohol)/poly(acrylic acid) polymer membranes in the absence of reducing agent and their effect on pervaporation of a water/acetic acid mixture. Bull Korean Chem Soc2016; 37(12): 1985–1991.
37.
BhattacharyaSSMishraAPalD, et al. Synthesis and characterization of poly(acrylic acid)/poly(vinyl alcohol)-xanthan gum interpenetrating network (IPN) superabsorbent polymeric composites. Polym-Plast Technol2012; 51(9): 876–882.
38.
ZhuMHuaDPanH, et al. Green electrospun and crosslinked poly(vinyl alcohol)/poly(acrylic acid) composite membranes for antibacterial effective air filtration. J Colloid Interf Sci2018; 511: 411–423.
39.
NelAXiaTMädlerL, et al. Toxic potential of materials at the nanolevel. Science2006; 311(5761): 622–627.
MarchioriMCLRigonCCopettiPM, et al. Nanoencapsulation improves scavenging capacity and decreases cytotoxicity of silibinin and pomegranate oil association. AAPS Pharm Sci Tech2017; 18(8): 3236–3246.
42.
Al-SabahiBNFatopeMOEssaMM, et al. Pomegranate seed oil: effect on 3-nitropropionic acid-induced neurotoxicity in PC12 cells and elucidation of unsaturated fatty acids composition. Nutr Neurosci2017; 20: 40–48.
43.
FerreiraLMGehrckeMCerviVP, et al. Pomegranate seed oil nanoemulsions with selective antiglioma activity: optimization and evaluation of cytotoxicity, genotoxicity and oxidative effects on mononuclear cells. Pharm Biol2016; 54(12): 2968–2977.
44.
LiRChenXGJiaK, et al. A systematic determination of polyphenols constituents and cytotoxic ability in fruit parts of pomegranates derived from five Chinese cultivars. SpringerPlus2016; 5(1): 914.
45.
NavarroMAmigo-BenaventMMesiasM, et al. An aqueous pomegranate seed extract ameliorates oxidative stress of human hepatoma HepG2 cells. J Sci Food Agric2014; 94(8): 1622–1627.
