In vitro evaluation of mucoadhesive and self-disinfection efficiency of (acrylic acid/polyethylene glycol)-silver nanocomposites for buccal drug delivery

Abstract

A mucoadhesive drug delivery system can improve the effectiveness of a drug, allowing targeting and localization at a specific site. According to this assumption, γ-irradiation as eco-friendly technique was employed to synthesize (acrylic acid/polyethylene glycol) copolymer hydrogel of different compositions. Silver nanoparticles were prepared within (acrylic acid/polyethylene glycol) hydrogel network by means of in situ reduction of silver nitrate using sodium borohydride as a reducing agent. Swelling characteristics in distilled water and simulated saliva solution were studied as a function of copolymer composition and preparation irradiation dose. (Acrylic acid/polyethylene glycol) hydrogels and their developed Agº nanocomposites have been characterized using scanning electron microscope, thermogravimetric analysis, transmission electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction. Mucoadhesive strength as well as self-disinfection efficiency expressed as antibacterial activity against different bacterial strains was evaluated. Propranolol HCl as model drug was used to evaluate the potential efficiency of the obtained (acrylic acid/polyethylene glycol)-Agº nanocomposites as mucoadhesive drug carrier. The obtained results showed that the (acrylic acid/polyethylene glycol)-Agº nanocomposites show a promising self-disinfection property, and the propranolol HCl–loaded composites were able to deliver the loaded drug in a sustainable manner that lasts for about 600 min.

Keywords

γ-irradiation silver nanocomposites mucoadhesive self-disinfection drug carrier

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References

Russo

Selmin

Baldassari

et al . A focus on mucoadhesive polymers and their application in buccal dosage forms. J Drug Deliv Sci Tec 2016; 32: 113–125.

Palacio

MLB

Bhushan

. Bioadhesion: a review of concepts and applications. Philos Trans A Math Phys Eng Sci 2012; 370: 2321–2347.

Smart

JD.

The basics and underlying mechanisms of mucoadhesion. Adv Drug Deliv Rev 2005; 57: 1556–1568.

Lee

W-F

Chen

Y-C.

Effect of bentonite on the physical properties and drug-release behavior of poly(AA-co-PEGMEA)/bentonite nanocomposite hydrogels for mucoadhesive. J Appl Polym Sci 2004; 91: 2934–2941.

Lee

VHL

. Mucosal drug delivery. JNCI Monogr 2001; 2001: 41–44.

Lopes

Bettencourt

Rossi

et al . Overview on gastroretentive drug delivery systems for improving drug bioavailability. Int J Pharm 2016; 510: 144–158.

Ribeiro

Rodrigues Filho

Meneguin

et al . Cellulose triacetate films obtained from sugarcane bagasse: evaluation as coating and mucoadhesive material for drug delivery systems. Carbohydr Polym 2016; 152: 764–774.

Abruzzo

Cerchiara

Bigucci

et al . Mucoadhesive buccal tablets based on chitosan/gelatin microparticles for delivery of propranolol hydrochloride. J Pharm Sci 2015; 104: 4365–4372.

Meka

Dharmanlingam

Kolapalli

VRM

. Formulation of gastroretentive floating drug delivery system using hydrophilic polymers and its in vitro characterization. Braz J Pharm Sci 2014; 50: 431–439.

10.

Guggi

Marschütz

Bernkop-Schnürch

Matrix tablets based on thiolated poly(acrylic acid): pH-dependent variation in disintegration and mucoadhesion. Int J Pharm 2004; 274: 97–105.

11.

Bonengel

Jelkmann

et al . Charge changing phosphorylated polymers: proof of in situ mucoadhesive properties. Eur J Pharm Biopharm 2016; 105: 203–208.

12.

Patel

Smart

Nevell

et al . Mucin/poly(acrylic acid) interactions: a spectroscopic investigation of mucoadhesion. Biomacromolecules 2003; 4: 1184–1190.

13.

Shalviri

Chan

Raval

et al . Design of pH-responsive nanoparticles of terpolymer of poly(methacrylic acid), polysorbate 80 and starch for delivery of doxorubicin. Colloids Surf B Biointerfaces 2013; 101: 405–413.

14.

Chandra Babu

Prabhakar

Suresh Babu

et al . Development and characterization of semi-IPN silver nanocomposite hydrogels for antibacterial applications. Int J Carbohydr Chem 2013; 2013: 8.

15.

Jalali

SAH

Allafchian

. Assessment of antibacterial properties of novel silver nanocomposite. J Taiwan Inst Chem E 2016; 59: 506–513.

16.

Motshekga

Ray

Onyango

et al . Preparation and antibacterial activity of chitosan-based nanocomposites containing bentonite-supported silver and zinc oxide nanoparticles for water disinfection. Appl Clay Sci 2015; 114: 330–339.

17.

W-R

Xie

X-B

Shi

Q-S

et al . Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Appl Microbiol Biotechnol 2010; 85: 1115–1122.

18.

Juby

Dwivedi

Kumar

et al . Silver nanoparticle-loaded PVA/gum acacia hydrogel: synthesis, characterization and antibacterial study. Carbohydr Polym 2012; 89: 906–913.

19.

El-Shishtawy

Asiri

Abdelwahed

NAM

et al . In situ production of silver nanoparticle on cotton fabric and its antimicrobial evaluation. Cellulose 2011; 18: 75–82.

20.

Zarei

Jamnejad

Khajehali

Antibacterial effect of silver nanoparticles against four foodborne pathogens. Jundishapur J Microbiol 2014; 7: e8720.

21.

Xiang

Chen

Preparation of a novel pH-responsive silver nanoparticle/poly(HEMA–PEGMA–MAA) composite hydrogel. Eur Polym J 2007; 43: 4178–4187.

22.

Di Giulio

Di Bartolomeo

Di Campli

et al . The effect of a silver nanoparticle polysaccharide system on streptococcal and saliva-derived biofilms. Int J Mol Sci 2013; 14: 13615–13625.

23.

Ganguly

Dash

AK.

A novel in situ gel for sustained drug delivery and targeting. Int J Pharm 2004; 276: 83–92.

24.

Al-Kassas

Wen

Cheng

AE-M

et al . Transdermal delivery of propranolol hydrochloride through chitosan nanoparticles dispersed in mucoadhesive gel. Carbohydr Polym 2016; 153: 176–186.

25.

Brett

CMA

Trandafir

. The corrosion of dental amalgam in artificial salivas: an electrochemical impedance study. J Electroanal Chem 2004; 572: 347–354.

26.

Wittaya-areekul

Prahsarn

Development and in vitro evaluation of chitosan–polysaccharides composite wound dressings. Int J Pharm 2006; 313: 123–128.

27.

Patel

Prajapati

Patel

MM.

Formulation, evaluation, and comparison of bilayered and multilayered mucoadhesive buccal devices of propranolol hydrochloride. AAPS PharmSciTech 2007; 8: E147–E154.

28.

Raafat

AI.

Gelatin based pH-sensitive hydrogels for colon-specific oral drug delivery: synthesis, characterization, and in vitro release study. J Appl Polym Sci 2010; 118: 2642–2649.

29.

Abd Alla

Nizam El-Din

El-Naggar

AWM.

Structure and swelling-release behaviour of poly(vinyl pyrrolidone) (PVP) and acrylic acid (AAc) copolymer hydrogels prepared by gamma irradiation. Eur Polym J 2007; 43: 2987–2998.

30.

Singh

Pal

Radiation crosslinking polymerization of sterculia polysaccharide-PVA-PVP for making hydrogel wound dressings. Int J Biol Macromol 2011; 48: 501–510.

31.

Ali

AE-H

Hegazy

E-SA

. Radiation synthesis of poly(ethylene glycol)/acrylic acid hydrogel as carrier for site specific drug delivery. J Biomed Mater Res B Appl Biomater 2007; 81: 168–174.

32.

Thomas

Yallapu

Sreedhar

et al . A versatile strategy to fabricate hydrogel–silver nanocomposites and investigation of their antimicrobial activity. J Colloid Interface Sci 2007; 315: 389–395.

33.

Nguyen

N-T

Liu

J-H.

A green method for in situ synthesis of poly(vinyl alcohol)/chitosan hydrogel thin films with entrapped silver nanoparticles. J Taiwan Inst Chem E 2014; 45: 2827–2833.

34.

Vimala

Samba Sivudu

Murali Mohan

et al . Controlled silver nanoparticles synthesis in semi-hydrogel networks of poly(acrylamide) and carbohydrates: a rational methodology for antibacterial application. Carbohydr Polym 2009; 75: 463–471.

35.

Davidenko

Carrodeguas

Peniche

et al . Chitosan/apatite composite beads prepared by in situ generation of apatite or Si-apatite nanocrystals. Acta Biomater 2010; 6: 466–476.

36.

Varaprasad

Mohan

Ravindra

et al . Hydrogel–silver nanoparticle composites: a new generation of antimicrobials. J Appl Polym Sci 2010; 115: 1199–1207.

37.

Del Fatti

Vallée

Flytzanis

et al . Electron dynamics and surface plasmon resonance nonlinearities in metal nanoparticles. Chem Phys 2000; 251: 215–226.

38.

Lau

A study of blending and complexation of poly(acrylic acid)/poly(vinyl pyrrolidone). Polymer 2002; 43: 823–829.

39.

Thomas

Yallapu

Sreedhar

et al . Fabrication, characterization of chitosan/nanosilver film and its potential antibacterial application. J Biomater Sci Polym Ed 2009; 20: 2129–2144.

40.

Mandal

Ray

SK.

Swelling, diffusion, network parameters and adsorption properties of IPN hydrogel of chitosan and acrylic copolymer. Mater Sci Eng C Mater Biol Appl 2014; 44: 132–143.

41.

Okay

Sariisik

SB.

Swelling behavior of poly(acrylamide-co-sodium acrylate) hydrogels in aqueous salt solutions: theory versus experiments. Eur Polym J 2000; 36: 393–399.

42.

Liu

Kim

H-I.

Characterization and antibacterial properties of genipin-crosslinked chitosan/poly(ethylene glycol)/ZnO/Ag nanocomposites. Carbohydr Polym 2012; 89: 111–116.

43.

Giacomelli

Spinelli

Behavior of a Co-Cr-Mo biomaterial in simulated body fluid solutions studied by electrochemical and surface analysis techniques. J Brazil Chem Soc 2004; 15: 541–547.

44.

Hebeish

Hashem

El-Hady

MMA

et al . Development of CMC hydrogels loaded with silver nano-particles for medical applications. Carbohydr Polym 2013; 92: 407–413.

45.

Rai

Yadav

Sinha

et al . Development of cellulosic polymer based gel of novel ternary mixture of miconazole nitrate for buccal delivery. Carbohydr Polym 2014; 103: 126–133.

46.

Mura

Cirri

Mennini

et al . Polymeric mucoadhesive tablets for topical or systemic buccal delivery of clonazepam: effect of cyclodextrin complexation. Carbohydr Polym 2016; 152: 755–763.

47.

Madsen

Eberth

Smart

JD.

A rheological assessment of the nature of interactions between mucoadhesive polymers and a homogenised mucus gel. Biomaterials 1998; 19: 1083–1092.

48.

Yehia

El-Gazayerly

Basalious

. Design and in vitro/in vivo evaluation of novel mucoadhesive buccal discs of an antifungal drug: relationship between swelling, erosion, and drug release. AAPS PharmSciTech 2008; 9: 1207–1217.

49.

Peppas

Buri

PA.

Surface, interfacial and molecular aspects of polymer bioadhesion on soft tissues. J Control Release 1985; 2: 257–275.

50.

Lele

Hoffman

AS.

Mucoadhesive drug carriers based on complexes of poly(acrylic acid) and PEGylated drugs having hydrolysable PEG-anhydride-drug linkages. J Control Release 2000; 69: 237–248.

51.

Lee

Park

Robinson

JR.

Bioadhesive-based dosage forms: the next generation. J Pharm Sci 2000; 89: 850–866.

52.

Das

Gang

Nath

SS.

Preparation and antibacterial activity of silver nanoparticles. J Biomater Nanobiotechnol 2011; 2(4): 4.

53.

Korsmeyer

Gurny

Doelker

et al . Mechanisms of solute release from porous hydrophilic polymers. Int J Pharm 1983; 15: 25–35.

54.

Raafat

Abd-Allah

WM.

In vitro apatite forming ability and ketoprofen release of radiation synthesized (gelatin-polyvinyl alcohol)/bioglass composite scaffolds for bone tissue regeneration. Polym Composite. Epub ahead of print 12 March 2016. DOI: 10.1002/pc.23974.