In this article, properties of thermosensitive chitosan hydrogels prepared with the use of chitosan chloride with β-glycerophosphate disodium salt pentahydrate enriched with calcium glycerophosphate are presented and compared with chitosan hydrogels with β-glycerophosphate disodium salt pentahydrate. The study is focused on the determination of hydrogel structure and biological testing of hydrogels with human osteoblasts line Saos-2. The structure of gels was visualized by scanning electron microscopy and was investigated by infrared spectroscopy. The crystallinity of gel structure was determined by X-ray diffraction analysis and thermal effects were determined using differential scanning calorimetry thermograms.
KavyaKCDixitRJayakumarR. Synthesis and characterization of chitosan/chondroitin sulfate/nano-SiO2 composite scaffold for bone tissue engineering. J Biomed Nanotechnol2012; 8: 149–160.
13.
VenkatesanJPallelaRBhatnagarI. Chitosan-amylopectin/hydroxyapatite and chitosan-chondroitin sulphate/hydroxyapatite composite scaffolds for bone tissue engineering. Int J Biol Macromol2012; 51: 1033–1042.
Il’inaAVVarlamovVP.Chitosan-based polyelectrolyte complexes: a review. Appl Biochem Microbiol2005; 41: 5–11.
16.
PiaiJFMartinsAda SilvaMA. Preparation and characterization of chitosan/chondroitin sulfate-based scaffold for cartilage regeneration. In: Proceedings of the XI meeting of SBPMat (Brazilian society of materials), Florianópolis, Brazil, 23–27 September 2012.
KimMSParkSJChunHJ. Thermosensitive hydrogels for tissue engineering. Tissue Eng Regen Med2011; 8: 117–123.
19.
ZhouHYJiangLJCaoPP. Glycerophosphate-based chitosan thermosensitive hydrogels and their biomedical applications. Carbohydr Polym2015; 117: 524–536.
20.
Thein-HanWWMisraRDK. Biomimetic chitosan–nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomater2009; 5: 1182–1197.
21.
LiBQHuQLWangM. Composite chitosan/nano-hydroxyapatite scaffolds induce osteocalcin production by osteoblasts in vitro and support bone formation in vivo. Tissue Eng Part A2009; 1, http://dx.doi.org/10.5772/48302
22.
MohamedKRBehereiHHEl-RashidyZM.In vitro study of nano-hydroxyapatite/chitosan-gelatin composites for bio-applications. J Adv Res2014; 5: 201–208.
23.
LeeYMParkYJLeeSJ. Tissue engineered bone formation using chitosan/tricalcium phosphate sponges. J Periodontol2000; 71: 410–417.
24.
YinYYeFCuiJ. Preparation and characterization of macroporous chitosan-gelatin/beta-tricalcium phosphate composite scaffolds for bone tissue engineering. J Biomed Mater Res A2003; 67: 844–855.
25.
MastrogiacomoMScaglioneSMartinettiR. Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics. Biomaterials2006; 27: 3230–3237.
26.
DessìMBorzacchielloAEl MassryTH. Novel biomimetic thermosensitive β-tricalcium phosphate/chitosan-based hydrogels for bone tissue engineering. J Biomed Mater Res A2013; 101: 2984–2993.
27.
NiranjanRKoushikCSaravananS. A novel injectable temperature-sensitive zinc doped chitosan/β-glycerophosphate hydrogel for bone tissue engineering. Int J Biol Macromol2013; 54: 24–29.
28.
SuWTChouWLChouCM.Osteoblastic differentiation of stem cells from human exfoliated deciduous teeth induced by thermosensitive hydrogels with strontium phosphate. Mater Sci Eng C Mater Biol Appl2015; 52: 46–53.
29.
DouglasTELSkwarczynskaAModrzejewskaZ. Acceleration of gelation and promotion of mineralization of chitosan hydrogels by alkaline phosphatase. Int J Biol Macromol2013; 56: 122–132.
30.
FoghJWrightWCLovelessJD.Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J Natl Cancer Inst1997; 58: 209–214.
31.
MosmannT.Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods1983; 65: 55–63.
32.
NociariMMShalevABeniasP. A novel one-step, highly sensitive fluorometric assay to evaluate cell-mediated cytotoxicity. J Immunol Methods1998; 213: 157–167.
33.
JiCKhademhosseiniADehghaniF.Enhancing cell penetration and proliferation in chitosan hydrogels for tissue engineering applications. Biomaterials2011; 32: 9719–9729.
