Sage Journals: Discover world-class research

Abstract

To make an implantable bioartificial liver (IBL), a new biocompatible collagen/chitosan/heparin complex was prepared using a crosslinking agent. The X-ray photoelectron spectroscopy (XPS), mechanical strength and biocompatibility with whole blood and hepatocytes were measured. The collagen/chitosan/heparin complex resulted in a superior blood compatibility compared to 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) crosslinked collagen matrix. The morphology and behavior of the cells on the collagen/chitosan/heparin membrane were found to be different from those on the collagen and collagen/chitosan membranes. Cells on the collagen membrane formed smaller three-dimensional aggregates than those on the collagen/chitosan membrane, while on the collagen/chitosan/heparin membrane, a round shape with no junctions were manifested. No adverse effects were found on the viability and function of the hepatocytes on the collagen/chitosan/heparin membrane compared to the collagen and collagen/chitosan membranes. These results suggest that this collagen/chitosan/heparin matrix is a potential candidate for hepatic tissue engineering.

Keywords

collagen/chitosan/heparin complex biodegradable biocompatibility hepatocyte scaffold tissue engineering

Get full access to this article

View all access options for this article.

References

Mikos, A.G. and Temenoff, J.S. (2000). Formation of Highly Porous Biodegradable Scaffolds for Tissue Engineering , EJB Electronic Journal of Biotechnology, 3(2): 114-119 .

Langer, R.S. and Vacanti, J.P. (1999). Tissue Engineering: The Challenges Ahead , Sci. Am., 4: 86-89 .

Kaihara, S. , Borenstein, J. , Koka, R. , Lalan, S. , Ochoa, E.R. , Ravens, M. , Pien, H. , Cunningham, B. and Vacanti, J.P. (2000). Silicon Micromachining to Tissue Engineer Branched Vascular Channels for Liver Fabrication , Tissue Eng., 6(2): 105-117 .

Edelman, E.R. , Nathan, A. , Katada, M. , Gates, J. and Karnovsky, M.J. (2000). Perivascular Graft Heparin Delivery Using Biodegradable Polymer Wraps , Biomaterials, 21: 2279-2286 .

Schor, A.M. , Schor, S.L. and Kumar, S. (1979). Importance of a Collagen Substratum for Stimulation of Capillary Endothelial Cell Proliferation by Tumor Angiogenesis Factor , Int. J. Cancer, 24: 225 .

Mori, T. , Okumura, M. , Matsuura, M. , Ueno, K. , Tokura, S. , Okamoto, Y. , Minami, S. and Fujinaga T. (1997). Effect of Chitin and its Derivatives on the Proliferation and Cytokine Production of Fibroblasts In Vitro , Biomaterials, 18: 947-951 .

Stemberger, A. , Ascherl, R. and Kollagen, G.B. (1990). Ein Biomaterial in der Medizin , Hämostaseologie, 10: 164-176 .

Wang, X.H. , Li, D.P. , Wang, W.J. , Feng, Q.L. , Cui, F.Z. , Xu, Y.X. and Song, X.H. (2003). Crosslinked Collagen/Chitosan Matrix for Artificial Livers , Biomaterials, 24: 3213-3220 .

Wang, X.H. , Cui, F.Z. , Feng, Q.L. , Li, J.C. and Zhang, Y.H. (2003). Preparation and Characterization of Collagen/Chitosan Matrices as Potential Biomaterials , J. Bioact. and Comp. Polym., 18(6): 453-467 .

10.

Gilles, M.A. , Hudson, A.Q. and Borders, C.L. (1990). Stability of Water-Soluble Carbodiimides in Aqueous Solutions , Anal. Biochem., 184: 244-248 .

11.

Seglen, P.O. (1976). Preparation of Isolated Rat Liver Cells , Methods Biol., 13: 29 .

12.

Ingber, D. , Karpp, S. , Plopper, G. , Hansed, L. and Mooney, D. (1993). Physical Forces and the Mammalian Cell, Academic Press, New York .

13.

Hill, G.E. (1998). Cardiopulmonary Bypass-Induced Inflammation: Is it Important? J. Cardiothorac. Vasc. Anesth., 12: 21-25 .

14.

Wendel, H.P. and Ziemer, G. (1999). Coating-Techniques to Improve the Hemocompatibility of Artificial Surfaces Used for Extracorporeal Circulation , Eur. J. Cardiothorac. Surg., 16: 342-350 .

15.

Miura, Y. , Akimoto, T. , Kanazawa, H. and Yagi, K. (1986). Synthesis and Secretion of Protein by Hepatocytes Entrapped within Calcium Alginate , Artif. Org., 10: 460-465 .

16.

Yin, C. , Ying, L. , Zhang, P.C. , Zhuo, R.X. , Kang, E.T. , Leong, K.W. and Mao, H.Q. (2003). High Density of Immobilized Galactose Ligand Enhances Hepatocyte Attachment and Function , J. Biomed. Mater. Res., 67: 1093-1104 .

Collagen/Chitosan/Heparin Complex with Improved Biocompatibility for Hepatic Tissue Engineering

Abstract

Keywords

Get full access to this article

References