Use of salicylic acid polymers and bone morphogenetic protein-2 to promote bone regeneration in rabbit parietal bone defects

Abstract

The ability of bone defects to heal spontaneously is inversely related to the size of the defect, such that defects larger than a critical size will not heal without additional therapeutic intervention. Typically, large bone defects are filled with autologous bone harvested from another skeletal site, an osteoconductive bone graft material, treated with an osteoinductive factor such as bone morphogenetic protein-2, or by a combination of these approaches. Despite these interventions, unsatisfactory success and complication rates show that alternative treatment methods are needed. Here, we test whether salicylic acid polymers can be used as guided bone regeneration barriers in conjunction with bone morphogenetic protein-2 to treat 1-cm-diameter defects in rabbit parietal bones. Porous, 1-cm round polycaprolactone scaffolds were infused with calcium sulfate–containing bone morphogenetic protein-2 and then capped on one side with salicylic acid polymers. The polymers slowed resorption of calcium sulfate that was used as a carrier for bone morphogenetic protein-2, indicating that bone morphogenetic protein-2 release into the parietal bone defect was extended by the use of the salicylic acid polymer. Microcomputerized tomography and histomorphometric analysis of the parietal bones 8 weeks after implantation showed that the salicylic acid polymer did not impair bone formation in the defect. These observations indicate that salicylic polymers paired with bone morphogenetic protein-2 can be optimized for use in guided bone regeneration to help repair large bone defects.