In this study, chitosan/polyvinyl alcohol binary and tannic acid/chitosan/polyvinyl alcohol ternary composite nanofiber membranes from chitosan citric acid salt aqueous solutions without the use of toxic solvents through Forcespinning® technology were successfully mass produced. Chitosan is being widely evaluated as a wound dressing material based on its biocompatibility, biodegradability, and antibacterial properties. Tannic acid has been applied as a drug for the treatment of wounds due to its astringent, antioxidant, hemostatic, and antibacterial properties. The ternary composite nanofiber membranes were in situ crosslinked to become water stable, in order to be used as wound dressings. The morphology, structure, water stability, water absorption capability, and thermal and mechanical properties of the composite nanofibers were characterized. The antibacterial activity was evaluated by the viable cell-counting method. The ternary composite nanofibers demonstrated synergistic antibacterial activity against Escherichia coli. The ternary composite membranes were found to be beneficial to the adhesion and development of fibroblast cells. These composite nanofiber membranes have the potential to be used in wound dressing applications.
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