Poly(2-ethyl-(2-pyrrolidone) methacrylate) and hyaluronic acid–based hydrogels for the engineering of a cartilage-like tissue using bovine articular chondrocytes
Restricted accessResearch articleFirst published online November, 2014
Poly(2-ethyl-(2-pyrrolidone) methacrylate) and hyaluronic acid–based hydrogels for the engineering of a cartilage-like tissue using bovine articular chondrocytes
Poly(2-ethyl-(2-pyrrolidone)methacrylate)–hyaluronic acid hydrogels based on the free radical polymerization of 2-ethyl-(2-pyrrolidone)methacrylate combined with hyaluronic acid, using N,N′-methylenebisacrylamide or triethylene glycol dimethacrylate, as cross-linking agents, were considered for tissue engineering applications. Bovine articular chondrocytes were seeded onto the poly(2-ethyl-(2-pyrrolidone)methacrylate)–hyaluronic acid hydrogels, under orbital agitation, for a total of 40 days. The engineered cell-constructs were characterized according to cell proliferation, morphology and distribution as well as the biochemical composition of the tissue formed. The chondrocytes were found to be attached and presented a typical spherical morphology. Cells were able to proliferate and synthesize a hyaline-like matrix rich in glycosaminoglycans and collagen type II which were mainly located on the superficial area. Increased content of individual components poly(2-ethyl-(2-pyrrolidone)methacrylate) and hyaluronic acid, in triethylene glycol dimethacrylate–cross-linked networks led to enhanced cell distribution and total glycosaminoglycans content, supporting their potential application for the repair of cartilaginous tissues.
Ruiz-RomeroCBlancoFJ.The role of proteomics in osteoarthritis pathogenesis research. Curr Drug Targets2009; 10(6): 543–556.
2.
LawrenceRCFelsonDTHelmickCG. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II. Arthritis Rheum2008; 58(1): 26–35.
3.
FelsonDTLawrenceRCDieppePA. Osteoarthritis: new insights. Part 1: the disease and its risk factors. Ann Intern Med2000; 133(8): 635–646.
4.
RoosEM.Joint injury causes knee osteoarthritis in young adults. Curr Opin Rheumatol2005; 17(2): 195–200.
5.
LohmanderLSOstenbergAEnglundM. High prevalence of knee osteoarthritis, pain, and functional limitations in female soccer players twelve years after anterior cruciate ligament injury. Arthritis Rheum2004; 50(10): 3145–3152.
6.
LutznerJKastenPGuntherKP. Surgical options for patients with osteoarthritis of the knee. Nat Rev Rheumatol2009; 5(6): 309–316.
7.
BrittbergMLindahlANilssonA. Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation. N Engl J Med1994; 331(14): 889–895.
8.
HangodyLKishGKarpatiZ. Mosaicplasty for the treatment of articular cartilage defects: application in clinical practice. Orthopedics1998; 21(7): 751–756.
9.
MithoeferKMcAdamsTWilliamsRJ. Clinical efficacy of the microfracture technique for articular cartilage repair in the knee: an evidence-based systematic analysis. Am J Sports Med2009; 37(10): 2053–2063.
10.
BekkersJEInklaarMSarisDB.Treatment selection in articular cartilage lesions of the knee: a systematic review. Am J Sports Med2009; 37(Suppl. 1): 148S–155S.
11.
SteinertAFGhivizzaniSCRethwilmA. Major biological obstacles for persistent cell-based regeneration of articular cartilage. Arthritis Res Ther2007; 9(3): 213.
12.
SittingerMBurmesterGR.Can engineered cartilage transplants be used for treating rheumatic diseases?Nat Clin Pract Rheumatol2006; 2(4): 172–173.
13.
BuckwalterJAMowVCRatcliffeA.Restoration of injured or degenerated articular cartilage. J Am Acad Orthop Surg1994; 2(4): 192–201.
14.
MankinHJ.The response of articular cartilage to mechanical injury. J Bone Joint Surg Am1982; 64(3): 460–466.
15.
TemenoffJSMikosAG.Review: tissue engineering for regeneration of articular cartilage. Biomaterials2000; 21(5): 431–440.
DehneTKarlssonCRingeJ. Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix-associated autologous chondrocyte transplantation. Arthritis Res Ther2009; 11(5): R133.
18.
KreuzPCMullerSOssendorfC. Treatment of focal degenerative cartilage defects with polymer-based autologous chondrocyte grafts: four-year clinical results. Arthritis Res Ther2009; 11(2): R33.
19.
PodskubkaAPovysilCKubesR. Treatment of deep cartilage defects of the knee with autologous chondrocyte transplantation on a hyaluronic acid ester scaffolds (Hyalograft C). Acta Chir Orthop Traumatol Cech2006; 73(4): 251–263.
20.
AndereyaSMausUGavenisK. First clinical experiences with a novel 3D-collagen gel (CaReS) for the treatment of focal cartilage defects in the knee. Z Orthop ihre Grenzgeb2006; 144(3): 272–280.
21.
NehrerSDorotkaRDomayerS. Treatment of full-thickness chondral defects with hyalograft C in the knee: a prospective clinical case series with 2 to 7 years’ follow-up. Am J Sports Med2009; 37(Suppl. 1): 81S–87S.
22.
BartlettWSkinnerJAGoodingCR. Autologous chondrocyte implantation versus matrix-induced autologous chondrocyte implantation for osteochondral defects of the knee: a prospective, randomised study. J Bone Joint Surg Br2005; 87(5): 640–645.
23.
OssendorfCKapsCKreuzPC. Treatment of posttraumatic and focal osteoarthritic cartilage defects of the knee with autologous polymer-based three-dimensional chondrocyte grafts: 2-year clinical results. Arthritis Res Ther2007; 9(2): R41.
24.
KhanWSHardinghamTE.Cartilage tissue engineering approaches applicable in orthopaedic surgery: the past, the present, and the future. J Stem Cells2012; 7(2): 97–104.
25.
FakhariABerklandC.Applications and emerging trends of hyaluronic acid in tissue engineering, as a dermal filler and in osteoarthritis treatment. Acta Biomater2013; 9(7): 7081–7092.
26.
BorzacchielloAGloriaAMayolL. Natural/synthetic porous scaffold designs and properties for fibro-cartilaginous tissue engineering. J Bioact Compat Polym2011; 26(5): 437–451.
BurdickJAPrestwichGD.Hyaluronic acid hydrogels for biomedical applications. Adv Mater2011; 23(12): H41–H56.
29.
BalazsEADenlingerJL.Viscosupplementa-tion: a new concept in the treatment of osteoarthritis. J Rheumatol Suppl1993; 39: 3–9.
30.
BarbucciRLamponiSBorzacchielloA. Hyaluronic acid hydrogel in the treatment of osteoarthritis. Biomaterials2002; 23(23): 4503–4513.
31.
XuejunXNettiPAAmbrosioL. Preparation and characterization of a hydrogel from low-molecular weight hyaluronic acid. J Bioact Compat Polym2004; 19(1): 5–15.
32.
RenY-JZhouZ-YCuiF-Z. Hyaluronic acid/polylysine hydrogel as a transfer system for transplantation of neural stem cells. J Bioact Compat Polym2009; 24(1): 56–62.
33.
RochaPMSantoVEGomesME. Encapsulation of adipose-derived stem cells and transforming growth factor-β1 in carrageenan-based hydrogels for cartilage tissue engineering. J Bioact Compat Polym2011; 26(5): 493–507.
34.
WeiY-TSunX-DXueX. Hyaluronic acid hydrogel modified with Nogo-66 receptor antibody and poly(L-Lysine) enhancement of adherence and survival of primary hippocampal neurons. J Bioact Compat Polym2009; 24(3): 205–219.
35.
MagalhãesJSousaRAManoJF. Synthesis and characterization of sensitive hydrogels based on semi-interpenetrated networks of poly[2-ethyl-(2-pyrrolidone) methacrylate] and hyaluronic acid. J Biomed Mater Res A2013; 101(1): 157–166.
36.
SantosJRAlvesNMManoJF.New thermo-responsive hydrogels based on poly (N-isopropylacrylamide)/hyaluronic acid semi-interpenetrated polymer networks: swelling properties and drug release studies. J Bioact Compat Polym2010; 25(2): 169–184.
37.
CrawfordADickinsonSC. Methods in Molecular Biology: Biopolymer methods in tissue engineering. New York, USA: Humana Press, 2004.
38.
KafienahWSimsTJ. Methods in Molecular Biology: Biopolymer methods in tissue engineering. New York, USA: Humana Press, 2004.
39.
FarndaleRWButtleDJBarrettAJ.Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta1986; 883(2): 173–177.
MagalhãesJCrawfordAHattonPV. Mineralization of porous hydrogels based on semi-interpenetrated networks of poly[2-ethyl(2-pyrrolidone) methacrylate] and hyaluronic acid in simulated body fluid. J Bioact Compat Polym2013; 28(5): 468–480.
42.
AlmarzaAJAthanasiouKA.Design characteristics for the tissue engineering of cartilaginous tissues. Ann Biomed Eng2004; 32(1): 2–17.
43.
OliveiraJTCrawfordAMundyJM. A cartilage tissue engineering approach combining starch-polycaprolactone fibre mesh scaffolds with bovine articular chondrocytes. J Mater Sci Mater Med2007; 18(2): 295–302.
44.
PelttariKWixmertenAMartinI.Do we really need cartilage tissue engineering?Swiss Med Wkly2009; 139(41–42): 602–609.
45.
SenköüASimsekASahinFI. Interaction of cultured chondrocytes with chitosan scaffold. J Bioact Compat Polym2001; 16(2): 136–144.
46.
CorreiaCRMoreira-TeixeiraLSMoroniL. Chitosan scaffolds containing hyaluronic acid for cartilage tissue engineering. Tissue Eng Part C Methods2011; 17(7): 717–730.
47.
AlvesdaSilvaMLCrawfordAMundyJM. Chitosan/polyester-based scaffolds for cartilage tissue engineering: assessment of extracellular matrix formation. Acta Biomater2010; 6(3): 1149–1157.
48.
ZhangQLuHKawazoeN. Preparation of collagen scaffolds with controlled pore structures and improved mechanical property for cartilage tissue engineering. J Bioact Compat Polym2013; 28(5): 426–438.
49.
BryantSJAnsethKS.Hydrogel properties influence ECM production by chondrocytes photoencapsulated in poly(ethylene glycol) hydrogels. J Biomed Mater Res2002; 59(1): 63–72.
50.
CugginoJCIgarzabalCIARuedaJC. Synthesis and characterization of new hydrogels through copolymerization of N-acryloyl-tris-(hydroxymethyl)aminomethane and different crosslinking agents. Eur Polym J2008; 44(11): 3548–3555.
51.
KangJYChungCWSungJH. Novel porous matrix of hyaluronic acid for the three-dimensional culture of chondrocytes. Int J Pharm2009; 369(1–2): 114–120.
52.
Da SilvaMACrawfordAMundyJ. Evaluation of extracellular matrix formation in polycaprolactone and starch-compounded polycaprolactone nanofiber meshes when seeded with bovine articular chondrocytes. Tissue Eng Part A2009; 15(2): 377–385.
53.
KuttyJKChoESoo LeeJ. The effect of hyaluronic acid incorporation on fibroblast spreading and proliferation within PEG-diacrylate based semi-interpenetrating networks. Biomaterials2007; 28(33): 4928–4938.
54.
SpillerKLMaherSALowmanAM.Hydrogels for the repair of articular cartilage defects. Tissue Eng Part B Rev2011; 17(4): 281–299.
