Microstructure,degradation and in vitro MG63 cells interactions of a new poly(ε-caprolactone),zein,and hydroxyapatite composite for bone tissue engineering
Restricted accessResearch articleFirst published online May, 2012
Microstructure,degradation and in vitro MG63 cells interactions of a new poly(ε-caprolactone),zein,and hydroxyapatite composite for bone tissue engineering
Novel biodegradable biomaterials were investigated for potential application in bone tissue engineering. The biomaterials were prepared by blending poly(ε-caprolactone) and thermoplastic zein, a corn protein, with or without the incorporation of hydroxyapatite particles. The biomaterials were characterized in vitro to assess the degradation in phosphate buffer saline for 56 days by monitoring weight change, morphology, wettability, and tensile properties. The interaction between the biomaterials and MG63 was evaluated by proliferation, morphological characterization, and osteogenic differentiation assays up to 28 days in in vitro cultures. The incorporation of thermoplastic zein within poly(ε-caprolactone) enhanced the hydrophilicity and degradability, while minor effects were observed after the inclusion of the hydroxyapatite particles. Compared to the neat poly(ε-caprolactone), the multiphase poly(ε-caprolactone)/thermoplastic zein–hydroxyapatite composite improved the osteogenic differentiation of MG63 cells and is being considered a candidate material for bone tissue engineering applications.
El KadyAMMohamedKREl-BassyouniGT. Fabrication, characterization and bioactivity evaluation of calcium pyrophosphate/polymeric biocomposites. Ceram Int2009; 35(7): 2933–2942.
2.
BoccacciniARErolMStarkWJ. Polymer/bioactive glass nanocomposites for biomedical applications: a review. Comput Sci Technol2010; 70(13): 1764–1776.
3.
ManoJFSilvaGAAzevedoHS. Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends. J R Soc Interface2007; 4(17): 999–1030.
4.
MuruganRRamakrishnaS. Development of nanocomposites for bone grafting. Comput Sci Technol2005; 65: 2385–2406.
5.
SantinMMorrisCStandenG. A new class of bioactive and biodegradable soybean-based bone fillers. Biomacromolecules2007; 8(9): 2706–2711.
6.
QuZWangHTangT. Evaluation of the zein/inorganics composite on biocompatibility and osteoblastic differentiation. Acta Biomater2008; 4: 1360–1368.
7.
TuJWangHLiH. The in vivo bone formation by mesenchymal stem cells in zein scaffolds. Biomaterials2009; 30(26): 4369–4376.
8.
LiuKHsiehF. Protein–protein interactions during high-moisture extrusion for fibrous meat analogues and comparison of protein solubility methods using different solvent systems. J Agric Food Chem2008; 56(8): 2681–2687.
9.
ZeinIHutmacherDWTanKC. Fused deposition modeling of novel scaffold architectures for tissue engineering applications. Biomaterials2002; 23(4): 1169–1185.
10.
PuppiDChielliniFPirasAM. Polymeric materials for bone and cartilage repair. Prog Polym Sci2010; 35(4): 403–440.
11.
ZhongZSunXS. Properties of soy protein isolate/polycaprolactone blends compatibilized by methylene diphenyl diisocyanate. Polymer2001; 42(16): 6961–6969.
12.
SalgadoAJSousaRAFragaJS. Effects of starch/polycaprolactone-based blends for spinal cord injury regeneration in neurons/glial cells viability and proliferation. J Bioact Compat Pol2009; 24(3): 235–248.
13.
SalernoAGuarnieriDIannoneM. Effect of micro- and macroporosity of bone tissue three-dimensional-poly(ε-caprolactone) scaffold on human mesenchymal stem cells invasion, proliferation, and differentiation in vitro. Tissue Eng Pt A2010; 16(8): 2661–2673.
14.
BorzacchielloAGloriaAMayolL. Natural/synthetic porous scaffold designs and properties for fibro-cartilaginous tissue engineering. J Bioact Compat Pol2011; 26(5): 437–451.
15.
SalernoAOlivieroMDi MaioE. Design of porous polymeric scaffolds by gas foaming of heterogeneous blends. J Mater Sci-Mater M2009; 20(10): 2043–2051.
16.
SalernoAOlivieroMDi MaioE. Design of novel three-phase PCL/TZ–HA biomaterials for use in bone regeneration applications. J Mater Sci-Mater M2010; 21(9): 2569–2581.
17.
RezwanKChenQZBlakerJJ. Biodegradable and bioactive porous polymer/inorganic composite scaffolds for bone tissue engineering. Biomaterials2006; 27(18): 3413–3431.
18.
TanPSTeohSH. Effect of stiffness of polycaprolactone (PCL) membrane on cell proliferation. Mat Sci Eng C-Biomim2007; 27(2): 304–308.
19.
ShiKKokiniJLHuangQ. Engineering zein films with controlled surface morphology and hydrophilicity. J Agric Food Chem2009; 57(6): 2186–2192.
20.
LeeJHRimNGJungHS. Control of osteogenic differentiation and mineralization of human mesenchymal stem cells on composite nanofibers containing poly[lactic-co-(glycolic acid)] and hydroxyapatite. Macromol Biosci2010; 10(2): 173–182.
21.
CorradiniEMattosoLHCGuedesCGF. Mechanical, thermal and morphological properties of poly(ε-caprolactone)/zein blends. Polym Advan Technol2004; 15(6): 340–345.
22.
WanYZLuoHHeF. Mechanical, moisture absorption, and biodegradation behaviours of bacterial cellulose fibre-reinforced starch biocomposites. Comput Sci Technol2009; 69(7–8): 1212–1217.
23.
ZhengZWeiYWangG. Surface properties of chitosan films modified with polycations and their effects on the behavior of PC12 cells. J Bioact Compat Pol2009; 24(1): 63–82.
24.
MeredithJCSormanaJKeselowskyBG. Combinatorial characterization of cell interactions with polymer surfaces. J Biomed Mater Res A2003; 66: 483–490.
25.
KunzlerTPDrobekTSchulerM. Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients. Biomaterials2007; 28(13): 2175–2182.
26.
NavarroMEngelEPlanellJA. Surface characterization and cell response of a PLA/CaP glass biodegradable composite material. J Biomed Mater Res A2008; 85(2): 477–486.
27.
SalernoAZeppetelliSDi MaioE. Processing/structure/property relationship of multi-scaled PCL and PCL–HA composite scaffolds prepared via gas foaming and NaCl reverse templating. Biotechnol Bioeng2011; 108(4): 963–976.
28.
DongJSunQWangJ. Basic study of corn protein, zein, as a biomaterial in tissue engineering, surface morphology and biocompatibility. Biomaterials2004; 25(19): 4691–4697.
29.
DeligianniDDKatsalaNDKoutsoukosPG. Effect of surface roughness of hydroxyapatite on human bone marrow cell adhesion, proliferation, differentiation and detachment strength. Biomaterials2001; 22(1): 87–96.
30.
BeşkardeşIGGümüşderelioğluM. Biomimetic apatite-coated PCL scaffolds: effect of surface nanotopography on cellular functions. J Bioact Compat Pol2009; 24(1): 63–82.
31.
ZhaoGRainesALWielandM. Requirement for both micron-and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography. Biomaterials2007; 28(18): 2821–2829.
