Nanocomposite thermogel for controlled release of small proteins

Abstract

A nanocomposite thermogel composed of Pluronic®-based multiblock copolymer and laponite nanoclay was developed to sustain delivery of low-molecular-weight proteins. The rapid release of low-molecular-weight proteins from multiblock copolymer thermogels has been a problem for sustained delivery but was solved by using nanocomposite thermogel. Lysozyme (M_w = 14,700), a relatively low-molecular-weight protein, was successfully loaded into and released from nanocomposite thermogel. In addition, interactions among multiblock copolymer, laponite, and lysozyme were studied in terms of gelation, micellization, particle size, and zeta potential. Critical micellization temperatures and sol–gel transition temperatures of multiblock copolymer solutions were lowered with laponite addition. Positively charged lysozyme was adsorbed onto anionic surface of laponite, which increased with an increase in the lysozyme concentration. Particle size and zeta potential of the laponite–lysozyme complex were also dependent on the lysozyme concentration. The nanocomposite thermogel sustained lysozyme release to 40 days, whereas lysozyme release from multiblock copolymer thermogel lasted for only 18 days. The structural stability of released lysozyme was confirmed by circular dichroism spectroscopy and differential scanning calorimetry.