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Abstract

The effect of various surface modifications, in conjunction with the intrinsic surface features of expanded polytetrafluoroethylene (ePTFE) fibers on endothelialization, were investigated. A multi-step surface modification strategy was applied to commercial ePTFE sutures and then the effect of surface topography and surface chemistry involving cell adhesive and cell-resistant molecules was evaluated towards endothelial cell adhesion and its spreading. N-hepthylamine plasma polymer (HApp) was deposited onto the ePTFE fiber surface and then carboxy-methyl-dextran (CMD) was covalently attached. Subsequently, GRGDS and GRGES peptides were covalently grafted onto the CMD graft layer. The micrometric and nanometric features of ePTFE were qualitatively examined by atomic force microscopy, and scanning electron microscopy. Human umbilical vein endothelial cells (HUVECs) were used to evaluate the in vitro cell adhesion on nonmodified fibers and the multi-step surface coatings. Cell adhesive molecules (HApp and RGD) enhanced the cell adhesion while cell-resistant molecules (CMD and RGE) and nonmodified fibers resisted cell adhesion. Therefore, only surface features have no effect on the HUVEC adhesion and that the surface chemistry is dominant in modulating HUVEC adhesion on ePTFE fibers.

Keywords

ePTFE fiber surface modification plasma polymer deposition CMD grafting GRGDS grafting AFM and SEM analysis endothelial cell response HUVEC adhesion modified fibers.

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References

Li, J. , Ding, M. , Fu, Q. , Tan, H. , Xie, X. and Zhong, Y. ( 2008). A Novel Strategy to Graft RGD Peptide on Biomaterials Surfaces for Endothelization of Small-diamater Vascular Grafts and Tissue Engineering Blood Vessel, J. Mater. Sci. Mater. Med., 19: 2595-2603.

Crombez, M. , Chevallier, P. , Gaudreault, R.C. , Petitclerc, E. , Mantovani, D. and Laroche, G. ( 2005). Improving Arterial Prosthesis Neo-endothelialization: Application of a Proactive VEGF Construct onto PTFE Surfaces, Biomaterials, 26: 7402-7409.

Chevallier, P. , Janvier, R. , Mantovani, D. and Laroche, G. (2005). In Vitro Biological Performances of Phosphorylcholine-grafted ePTFE Prostheses through RFGD Plasma Techniques, Macromol. Biosci., 5: 829-839.

Hadjizadeh, A. , Doillon, C.J. and Vermette, P. (2007). Bioactive Polymer Fibers to Direct Endothelial Cell Growth in a Three-dimensional Environment, Biomacromolecules, 8: 864-873.

Curtis, A. and Wilkinson, C. ( 1997). Topographical Control of Cells, Biomaterials , 18: 1573-1583.

Andersson, A.S. , Backhed, F. , von Euler, A. , Richter-Dahlfors , A. , Sutherland, D. and Kasemo, B. (2003). Nanoscale Features Influence Epithelial Cell Morphology and Cytokine Production , Biomaterials, 24: 3427-3436.

Tan, J. and Saltzman, W.M. ( 2002). Topographical Control of Human Neutrophil Motility on Micropatterned Materials with Various Surface Chemistry, Biomaterials, 23: 3215-3225.

Flemming, R.G. , Murphy, C.J. , Abrams, G.A. , Goodman, S.L. and Nealey, P.F. (1999). Effects of Synthetic Micro- and Nano-structured Surfaces on Cell Behavior, Biomaterials , 20: 573-588.

Ibrahim, S. , Joddar, B. , Craps, M. and Ramamurthi, A. ( 2007). A Surface-tethered Model to Assess Size-specific Effects of Hyaluronan (HA) on Endothelial Cells, Biomaterials, 28: 825-835.

10.

Barbucci, R. , Lamponi, S. , Magnani, A. and Pasqui, D. ( 2002). Micropatterned Surfaces for the Control of Endothelial Cell Behaviour, Biomol. Eng., 19: 161-170.

11.

Li, Q.L. , Huang, N. , Chen, J. , Chen, C. , Chen, J. and Chen, H. ( 2009). Endothelial Cell and Platelet Behavior on Titanium Modified with a Mutilayer of Polyelectrolytes, J. Bioact. Compat. Polym. , 24: 129-150.

12.

Liu, Y. , Sun, S. , Singha, S. , Cho, M.R. and Gordon, R.J. ( 2005). 3D Femtosecond Laser Patterning of Collagen for Directed Cell Attachment, Biomaterials, 26: 4597-4605.

13.

Schnell, E. , Klinkhammer, K. , Balzer, S. , Brook, G. , Klee, D. , Dalton, P. et al. (2007). Guidance of Glial Cell Migration and Axonal Growth on Electrospun Nanofibers of Poly-epsilon-caprolactone and a Collagen/ Poly-epsilon-caprolactone Blend, Biomaterials , 28(19): 3012-3025.

14.

Senesi, G.S. , D’Aloia, E. , Gristina, R. , Favia, P. and d’Agostino , R. (2007). Surface Characterization of Plasma Deposited Nano-structured Fluorocarbon Coatings for Promoting In Vitro Cell Growth, Surf. Sci., 601: 1019-1025.

15.

Barbucci, R. , Lamponi, S. , Magnani, A. , Piras, F.M. , Rossi, A. and Weber, E. (2005). Role of the Hyal-Cu (II) Complex on Bovine Aortic and Lymphatic Endothelial Cells Behavior on Microstructured Surfaces, Biomacromolecules, 6: 212-219.

16.

Miller, D.C. , Thapa, A. aren Haberstroh , K.M. and Webster, T.J. (2004). Endothelial and Vascular Smooth Muscle Cell Function on Poly (lactic-co-glycolic acid) with Nano-structured Surface Features, Biomaterials , 25(1): 53-61.

17.

Chung, T.W. , Liu, D.Z. , Wang, S.Y. and Wang, S.S. (2003). Enhancement of the Growth of Human Endothelial Cells by Surface Roughness at Nanometer Scale , Biomaterials, 24: 4655-4661.

18.

Lofas, S. and Johnsson, B. ( 1990). A Novel Hydrogel Matrix on Gold Surfaces in Surface-plasmon Resonance Sensors for Fast and Efficient Covalent Immobilization of Ligands , J. Chem. Soc. Chem. Commun., 21: 1526-1528.

19.

Barbucci, R. , Pasqui, D. , Wirsen, A. , Affrossman, S. , Curtis, A. and Tetta, C. (2003). Micro and Nano-structured Surfaces, J. Mater. Sci. Mater. Med., 14: 721-725.

20.

Pasqui, D. , Rossi, A. , Barbucci, R. , Lamponi, S. , Gerli, R. and Weber, E. ( 2005). Hyaluronan and Sulphated Hyaluronan Micropatterns: Effect of Chemical and Topographic Cues on Lymphatic Endothelial Cell Alignment and Proliferation, Lymphology, 38: 50-65.

Endothelial Cell Responses Towards Surface-modified Expanded Polytetrafluoroethylene Fibers

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