In this in vitro investigation of a heterogeneous range of bio-functional and modulatory performances of mesenchymal stem cells (MSCs) grown on two plasma-deposited acrylic acid (pdAA) coatings, which differed in chemical characteristics, a view of their potential utility as hybrid scaffolds for bone tissue engineering was obtained. The in vitro behavior of MSCs was compared to that of MG-63 cells, an osteoblast-like cell line that is commonly used to test biocompatibility of materials intended for bone-tissue interface. The coatings exerted a greater stimulus on MSCs and on MG-63 cells in comparison with control cultures. Both studied coatings exhibited satisfactory compatibility and modulatory effects on MSCs, thus they may be suitable for use in 2D or 3D scaffolds for bone tissue reconstruction.
Huss, R. and Moosmann, S. (2002). The Co-Expression of CD117 (c-kit) and Osteocalcin in Activated Bone Marrow Stem Cells in Different Diseases , Brit. J. Haemathol., 118: 305-312 .
4.
Harris, C.T. and Cooper, L.F. (2004). Comparison of Bone Graft Matrices for Human Mesenchymal Stem Cell-Directed Osteogenesis , J Biomed. Mater. Res., 15(68 A): 747-755 .
5.
d’Agostino, R. (ed.) (1990). Plasma Deposition, Treatment and Etching of Polymers. In Plasma-Material Interaction Series, vol. 496. Academic Press, San Diego .
6.
d’Agostino, R. , Favia, P. and Fracassi, F. (1997). Plasma-Processing of Polymers, eds, Kluwer Academic Publisher , NATO ASI Series, E: Applied Sciences, Vol. 346.
7.
Ratner, B.D. , Chilchoti, A. and Lopez, G.P. (1990). Plasma Deposition and Treatment for Biomaterial Applications. In Plasma Deposition, Treatment and Etching of Polymers, d’Agostino, R. (ed.), p. 496, Academic Press, San Diego .
8.
Favia, P. , Sardella, E. , Gristina, R. , Milella, A. and d’Agostino, R. (2002). Fictionalization of Biomedical Polymers by Means of Plasma Processes: Plasma Treated Polymers with Limited Hydrophobic Recovery and PECVD of —COOH functional coatings , J. Photopolym. Sci. Tech.15: 341-350 .
9.
Favia P. , Sardella E. , Gristina R. and d’Agostino R. (2003). Novel plasma processes for biomaterials: micro-scale patterning of biomedical polymers . Surf. Coat. Tech., 169: 707-711 .
10.
Favia, P. and d’Agostino, R. (2002). Plasma Processed Surfaces for Biomaterials and Biomedical Devices: PEO-Like, Ag/PEO-Like and —COOH Functional Coatings, Micro-Patterned Surfaces. Le Vide, 203-1/4, 1.
11.
Palumbo, F. , Favia, P. , Rinaldi, A. , Vulpio, M. and d’Agostino, R. (1999). PE-CVD of Organic Thin Films with Controlled Surface Density of Carboxylic Groups , Plasmas and Polymers, 4: 133 .
12.
Detomaso, L. , Gristina, R. , Senesi, G.S. , d’Agostino, R. and Favia, P. (2005) PlasmA-Deposited Acrylic Acid: Characterization and Interaction with Human Fibroblasts . Biomaterials, 26: 3831-3841 .
13.
Anselme, K. (2000). Osteoblast Adhesion to Materials , Biomaterials, 21: 667-681 .
14.
Torricelli, P. , Fini, M. , Giavaresi, G. , Borsari, V. , Carpi, A. , Nicolini, A. and Giardino, R. (2003). Comparative Interspecies Investigation on Osteoblast Cultures: Data on Cell Viability and Synthetic Activity . Biomed. Pharmacother., 57(1): 57-62 .
15.
Suh, H. , Han, D.W. , Park, J.C. , Lee, D.H. , Lee, W.S. and Han, C.D. (2001). A Bone Replaceable Artificial Bone Substitute: Osteoinduction by Combining with Bone Inducing Agent . Artif. Organs, 25(6): 459-466 .
16.
Etienne-Manneville, S. , Hall, A. (2002). Rho GTPases in Cell Biology , Nature, 429: 629-635 .
17.
Hoffman, A.S. (1996). Biologically Functional Materials. In: Ratner, B.D. , Hoffman, A.S. , Shoen, F.J. and Lemons, J.E. (eds), Biomaterials Science: An Introduction to Materials in Medicine, pp. 124-130, Academic Press, New York .
18.
Beauvais-Jouneau, A. , Delouvee, A. , Craig, S.E. , Humphries, M.J. , Thiery, J.P. and Dufour, S. (1997). Direct Role of the Carboxy-Terminal Cell-Binding Domain of Fibronectin in Neural Crest Cell Motilità , Exp. Cell Res., 233: 1-10 .
19.
Pender, S.L. , Salmela, M.T. , Monteleone, G. , Schnapp, D. , McKenzie, C. , Spencer, J. , Fong, S. , Saarialho-Kere, U. and MacDonald, T.T. (2000). Ligation of Alpha4ss1 Integrin on Human Intestinal Mucosal Mesenchymal Cells Selectively Up-Regulates Membrane Type-1 Matrix Metalloproteinase and Confers a Migratory Phenotype , Am. J. Pathos., 157: 1955-1962 .
20.
Fazzi, R. , Galimberti, S. , Testi, R. , Pacini, S. , Trasciatti, S. , Rosini, S. and Petrini, M. (2002). Bone and Bone Marrow Interactions: Hematological Activity of Osteoblastic Growth Peptide (OGP)-Derived Carboxy-Terminal Pentapeptide. II. Action on Human Hematopoietic Stem Cells , Leuk. Res., 26: 839-848 .
21.
Montanaro, L. , Arciola, C.R. , Campoccia, D. and Cervellati, M. (2002). In Vitro Effects on MG63 Osteoblast-Like Cells Following Contact with Two Roughness-Differing Fluorohydroxyapatite-Coated Titanium Alloys , Biomaterials, 23: 3651-3659 .
22.
Zhang, Y. and Zhang, M. (2002). Three-Dimensional Macroporous Calcium Phosphate Bioceramics with Nested Chitosan Sponges for Load-Bearing Bone Implants , J. Biomed. Mater. Res., 61: 1-8 .
23.
Shin, H. , Zygourakis, K. , Farach-Carson, M.C. , Yaszemski, M.J. and Mikos, A.G. (2004). Modulation of Differentiation and Mineralization of Marrow Stromal Cells Cultured on Biomimetic Hydrogels Modified with Arg-Gly-Asp Containing Peptides , J. Biomed. Mater. Res., 69: 535-543 .
24.
Hardikar, A.A. , Marcus-Samuels, B. , Geras-Raaka, E. , Raaka, B.M. and Gershengorn, M.C. (2003). Human Pancreatic Precursor Cells Secrete FGF2 to Stimulate Clustering into Hormone-Expressing Islet-Like Cell Aggregates , Proc. Nat. Acad. Sci., 100: 7117-7122 .
25.
Shimazui, T. , Schalken, J.A. , Kawai, K. , Kawamoto, R. , van Bockhoven, A. , Oosterwijk, E. , Akaza, H. and Shimazui, T. (2004). Role of Complex Cadherins in Cell-Cell Adhesion Evaluated by Spheroid Formation in Renal Cell Carcinoma Cell Lines , Oncol. Rep., 118: 357-360 .
26.
Shen, H. , Tan, J. and Saltzman, W.M. (2004). Surface Mediated Gene Transfer from Nanocomposites of Controlled Texture , Nature Materials, 3: 569-574 .
27.
Ma, Z. , Gao, C. , Gong, Y. and Shen, J. (2005). Cartilage Tissue Engineering PLLA Scaffold with Surface Immobilised Collagen and Basic Fibro-blast Growth Factor , Biomaterials, 26: 1253-1259 .
